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CHAUNCEY,
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Circle No. 158 on Readers' Service Card
NEW BOOKS ceedingly brief or somewhat overbear ing. The authors compulsively stray into spheres that are far afield from the realm of biochemical applications. The first and the most extensive chapter of the text entitled "General Tech niques, Conventions and Instrumenta tion" represents an ambitious attempt to present a condensed version of cer tain major aspects of the theory and practice of gas chromatography. In several places it is woefully out of date. The majority of chapters that are concerned primarily with the applica tions of the technique are superbly pre sented. They are written with a degree of clarity and organization rarely seen in other books of this type. Editorial comment is generously interspersed with a wealth of information extracted from the pertinent scientific literature. Undoubtedly to the knowledgeable sci entist in the field this text will be of great service as an exceedingly handy source1 of reference material. The be ginner in gas chromatography however may do well to first approach a more condensed primer before moving on to a text of this type. Under these cir cumstances he is less likely to be over whelmed by the enormity of it all and will undoubtedly be in a better position to appreciate the valuable contribution made by Drs. Burchfield and Storrs. Fluorescence Assay in Biology and Medicine. Sidney Udenfriend. χ -+505 pages. Academic Press, Inc., Ill Fifth Ave., New York 3, Ν. Υ. 1962.
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The past decade has seen a tremen dous increase in the application of fluorometric assay methods in the bio logical and medical sciences. This has been due not only to the extraordinary sensitivity of such methods, but also to the recent development of accurate and reliable instruments which are available commercially. Unfortunately, as Dr. Udenfriend points out r biologists (in the broad sense) are often ill-equipped to know the pitfalls inherent in fluoro metric analysis, to select the most ap propriate methods, and to recognize all the theoretical implications of their findings. This book is intended as a practical guide through such problems. The first two chapters present a brief introduction to the terminology and theoretical basis of fluorometric analysis, including such topics as elec tronic transitions, Raman effect, fluo rescence efficiency, and molecular struc tural basis of fluorescence. Portions of other chapters deal with theoretical
