Stabilized Nanoscale Zerovalent Iron Mediated Cadmium

Aug 29, 2017 - Nanoparticles can be absorbed by plants, but their impacts on phytoremediation are not yet well understood. This study was carried out ...
2 downloads 11 Views 2MB Size
Subscriber access provided by UNIVERSITY OF ADELAIDE LIBRARIES

Article

Stabilized nanoscale zero-valent iron mediated cadmium accumulation and oxidative damage of Boehmeria nivea (L.) Gaudich cultivated in cadmium contaminated sediments Xiaomin Gong, Danlian Huang, Yunguo Liu, Guangming Zeng, Rongzhong Wang, Jia Wan, Chen Zhang, Min Cheng, Xiang Qin, and Wenjing Xue Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.7b03164 • Publication Date (Web): 29 Aug 2017 Downloaded from http://pubs.acs.org on August 31, 2017

Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a free service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are accessible to all readers and citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.

Environmental Science & Technology is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.

Page 1 of 40

Environmental Science & Technology

1

Stabilized nanoscale zero-valent iron mediated cadmium accumulation and

2

oxidative damage of Boehmeria nivea (L.) Gaudich cultivated in cadmium

3

contaminated sediments

4

Xiaomin Gong,†,‡ Danlian Huang,*,†,‡ Yunguo Liu,*,†,‡ Guangming Zeng,†,‡

5

Rongzhong Wang,†,‡ Jia Wan,†,‡ Chen Zhang†,‡ Min Cheng,†,‡ Xiang Qin,†,‡ Wenjing

6

Xue†,‡

7 8 9



College of Environmental Science and Engineering, Hunan University, Changsha

410082, China ‡

Key Laboratory of Environmental Biology and Pollution Control (Hunan

10

University), Ministry of Education, Changsha 410082, China

11

*

12

E-mail address: [email protected] (Dan-lian Huang)

13 14

Corresponding authors:

[email protected] (Yun-guo Liu) Tell: +86-13017295768; fax: +86-731-88823701

1

ACS Paragon Plus Environment

Environmental Science & Technology

15

ABSTRACT: Nanoparticles can be absorbed by plants, but their impacts on

16

phytoremediation are not yet well understood. This study was carried out to determine

17

the impacts of starch stabilized nanoscale zero-valent iron (S-nZVI) on the cadmium

18

(Cd) accumulation and the oxidative stress in Boehmeria nivea (L.) Gaudich (ramie).

19

Plants were cultivated in Cd-contaminated sediments amended with S-nZVI at 100,

20

500, and 1000 mg/kg, respectively. Results showed that S-nZVI promoted Cd

21

accumulation in ramie seedlings. The subcellular distribution result showed that Cd

22

content in cell wall of plants reduced, and its concentration in cell organelle and

23

soluble fractions increased at S-nZVI treatments, indicating the promotion of Cd

24

entering plant cells by S-nZVI. In addition, the 100 mg/kg S-nZVI alleviated the

25

oxidative damage to ramie under Cd-stress, while 500 and 1000 mg/kg S-nZVI

26

inhibited plant growth and aggravated the oxidative damage to plants. These findings

27

demonstrate that nanoparticles at low concentration can improve the efficiency of

28

phytoremediation. This study herein develops a promising novel technique by the

29

combined use of nanotechnology and phytoremediation in the remediation of heavy

30

metal contaminated sites.

2

ACS Paragon Plus Environment

Page 2 of 40

Page 3 of 40

31

Environmental Science & Technology

TOC/Abstract art:

32

3

ACS Paragon Plus Environment

Environmental Science & Technology

Page 4 of 40

33

INTRODUCTION

34

River sediments contaminated with heavy metals have become a widespread

35

environmental concern because it is toxic to aquatic organisms and likely to cause

36

water pollution. 1-3 Cadmium (Cd) contaminated sediments is becoming increasingly

37

common due to the wide application of Cd in electroplating, metallurgy, and

38

agriculture. Cd is a highly toxic heavy metal and can be bio-accumulated through

39

food chain, which poses potential risks to the natural ecosystem and human health. 4-6

40

Consequently,

41

Phytoremediation has been considered to be a cost-efficient technology for sediments

42

remediation since it can remove the toxic substances from sediments, especially in the

43

dry seasons of river. 7,8

remediation

of

Cd-contaminated

sediments

is

imperative.

44

Nanomaterials are extensively used, which will inevitably have potential impacts

45

on plants. For example, carbon nanotubes increased seeds germination rate and

46

promoted the seedlings growth of rice plants;

47

promoted by single-wall carbon nanotubes. 10 Some researchers reported the positive

48

effects as mentioned above, whereas others found the negative effects of

49

nanomaterials on plants. A study conducted by Lin and Xing

50

elongation of corn was reduced by 35% at alumina nanomaterials treatments.

51

Fullerene nanoparticles at high concentrations impeded plant development by

52

interfering the uptake of water and nutrients.

53

nanomaterials on plants certainly will affect the phytoremediation of the contaminated

54

environment.

9

12

plant photosynthetic activity was

11

found that root

The potential different effects of

4

ACS Paragon Plus Environment

Page 5 of 40

Environmental Science & Technology

55

Nanomaterials have been applied to decontaminate pollutants in the last few

56

years. 13-18 Recently, the combination of nanotechnology and phytoremediation to deal

57

with different kinds of soil pollution has gained increasing concerns. 19 A previous

58

study has shown that graphene oxide nanoparticles can serve as Cd carriers to

59

promote the uptake of Cd in Microcystis aeruginosa. 20 Panicum maximum growing in

60

the soil amended with 100, 500, and 1000 mg/kg nanoscale zero-valent iron (nZVI)

61

resulted in higher trinitrotoluene accumulation.

62

promoted polychlorinated biphenyls accumulation and facilitated plant growth of

63

Impatiens balsamina, thus enhancing the removal efficiency of soil contaminants. 22

21

nZVI at 750 and 1000 mg/kg

64

nZVI is a commonly used nanomaterial which has been widely applied for the

65

remediation of metal contaminated soils and sediments. nZVI has high capacities for

66

the removal/stabilization/degradation of environmental pollutants and can be

67

separated by an external magnetic field. 23-24 For example, nZVI immobilized Cd in

68

river sediments by transforming the mobile fraction of Cd into residual forms. 25 The

69

interactions between nZVI and metals probably will affect the bioavailability of

70

metals in plants. For example, nZVI at the dose of 1% and 10% were demonstrated to

71

reduce the availability of arsenic (As) in soil and decrease the uptake of As in

72

Hordeum vulgare L. cv. Pedrezuela;

73

immobilization and decreased its bioaccumulation in Chinese cabbage. 27 In addition,

74

previous reports 28,29 have confirmed the uptake of nZVI in plants. Since Cd can be

75

adsorbed on iron hydroxides through physical sorption and surface complexation, 30 it

76

is unclear whether nZVI can act as metal carriers to promote Cd accumulation and

26

stabilized nZVI enhanced Cr(VI)

5

ACS Paragon Plus Environment

Environmental Science & Technology

Page 6 of 40

77

translocation in plants or it will immobilize Cd and reduce its bioavailability to plants.

78

Reactive oxygen species (ROS) are generated in plants as byproducts of aerobic

79

metabolism (e.g., plant respiration and photosynthesis), which mainly include

80

hydrogen peroxide (H2O2), hydroxyl radicals (OH•) and superoxide radicals (O2•-).

81

The generation and scavenging of ROS in plants are balanced under general

82

environmental conditions. Heavy metals, such as Cd, will produce excess ROS in

83

plants, which may cause oxidative damage to proteins, DNA, and lipids. Previous

84

studies have shown that nZVI can produce ROS through iron oxidation or Fenton

85

reaction according to the following reactions: 31,32 𝐹𝑒 0 + 𝑂2 + 2𝐻 + = 𝐻2 𝑂2 + 𝐹𝑒 2+

(1)

𝐹𝑒 2+ + 𝐻2 𝑂2 = 𝑂𝐻• + 𝐹𝑒 3+ + 𝑂𝐻 −

(2)

𝐹𝑒 2+ + 𝑂2 = 𝑂2 •- + 𝐹𝑒 3+

(3)

86

In the presence of dissolved oxygen, iron is oxidized with the production of H2O2

87

(reaction 1). Furthermore, H2O2 converts to OH• through Fenton reaction (reaction 2).

88

The generation of O2•- can be achieved through ferrous ion oxidation (reaction 3).

89

The nZVI-induced ROS generation or conversion may modify the oxidative stress in

90

plants. However, it is unclear whether nZVI will aggravate Cd induced oxidative

91

stress in plants. Moreover, the over-production of ROS can be scavenged by

92

anti-oxidative enzymes and some antioxidants. Previous investigations revealed that

93

nanoparticles could modify the activities of enzymes related to the removal of ROS.

94

For instance, cerium oxide nanoparticles were found to increase catalase (CAT)

95

activity and reduce ascorbate peroxidase activity in cucumber. 33 nZVI changed the 6

ACS Paragon Plus Environment

Page 7 of 40

Environmental Science & Technology

96

activities of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) in

97

Escherichia coli cells. 34 Furthermore, a study conducted by Chaithawiwat et al. even

98

showed that nZVI particles performed roles in the expression of genes encoding

99

anti-oxidative enzymes. 35 Such nanoparticles induced modification may change the

100

anti-oxidative responses of plants exposed to heavy metals. To our knowledge, very

101

few studies have investigated the impacts of nZVI on the antioxidant defense systems

102

in plants under Cd-stress.

103

The current study focuses on the impacts of nZVI on the uptake and

104

translocation of Cd in plants. In addition, the oxidative damage and the anti-oxidative

105

defense of plants exposed to nZVI under Cd stress were investigated. Because of the

106

highly reactivity, nZVI tended to either be oxidized or agglomerate. Previous studies

107

have shown that starch can serve as stabilizer and dispersant to prevent nZVI from

108

agglomeration and oxidation. 36 Thus, starch stabilized nZVI (S-nZVI) was used as

109

the nanomaterial in the present experiments. In addition, our previous study revealed

110

that Bechmeria nivea (L.) Gaud. (Ramie) is a promising species for the

111

phytoremediation of Cd-contaminated environment due to its high Cd accumulation

112

ability and large biomass. 37 Therefore, ramie seedlings were chosen as the test plants.

113

In the present study, plant dry weight (DW), metal concentrations, ROS levels,

114

lipid peroxidation, anti-oxidative enzymes activities, reduced glutathione (GSH) and

115

oxidized glutathione (GSSG) contents in ramie were determined. Assessing the effects

116

of nanoparticles on plants under metal stress will provide new insights into the

117

combined use of nanotechnology and phytoremediation in the remediation of polluted 7

ACS Paragon Plus Environment

Environmental Science & Technology

118

environment, as well as the potential risks of nanoparticles to plants under metal

119

stress.

120

EXPERIMENTAL SECTION

121 122

Sediments Characterization. The preparation and characterization of sediments are presented in the Supporting Information.

123

Nanoparticles Synthesis and Characterization. nZVI and S-nZVI were

124

synthesized by the borohydride reduction method (laboratory preparation and

125

characterization of nanomaterials are described in the Supporting Information). 38

126

Plant Growth. Ramie seedlings were obtained from the Ramie Institute of

127

Hunan agriculture university, China. Before transplanting, Cd-contaminated

128

sediments were mixed with nZVI and S-nZVI thoroughly to obtain 0, 100, 500 and

129

1000 mg/kg nanoparticles contained Cd-contaminated sediments. Purified water was

130

added to the sediments to maintain sediments moisture of 80% field capacity. Then

131

ramie seedlings were transplanted into the above substrates and cultivated in a growth

132

chamber at 14 hours photoperiod (6:00 to 20:00), 24 ± 5 °C and 18 ± 3 °C day

133

and night temperature, and 65 ± 5% relative humidity. The selected nanoparticles

134

contents were based on the results from our pre-experiments, which showed that

135

concentrations1000 mg/kg significantly inhibited ramie seedlings growth.

137

Ramie seedlings were cultivated in the growth chamber for one month. Plants

138

grown in the only Cd-contaminated sediments without nanoparticles were used as the

139

control. All the treatments were performed in 4 replicates. After one month cultivation, 8

ACS Paragon Plus Environment

Page 8 of 40

Page 9 of 40

Environmental Science & Technology

140

plants were harvested. To determine the effects of nanoparticles on plant growth under

141

metal stress, ramie leaves, stems and roots were collected separately and dried in oven

142

at 70 °C till constant weight to record the biomass.

143

For nZVI treated plants, plant biomass and Cd concentration were determined.

144

Metal Analysis. To assess Cd and Fe contents and their translocation in plants,

145

ramie leaves, stems and roots were harvested separately and washed with purified

146

water thoroughly. For ramie roots, after washing with purified water, they were

147

soaked in 10 mM Na2EDTA (ethylenediaminetetraacetic acid disodium salt) for 15

148

min to remove metal ions (especially Cd and Fe ions) adhering to the root surface, 39,40

149

and then rinsed 3 times with purified water. All the washed tissues were dried till

150

constant weight, and then digested with a mixture of 10 mL HNO3 and 3 mL HClO4

151

using a graphite digestion apparatus (DS-360). Cd and Fe contents in different plant

152

tissues were analyzed by an atomic absorption spectrometer (AAS, PEAA700,

153

PerkinElmer, U.S.A.). The amount of Cd accumulated in each plant (A-Cd) and total

154

Cd concentration (T-Cd) in ramie are calculated as follows:

A-Cd = 𝐶𝑑𝐿 × 𝐵𝐿 + 𝐶𝑑𝑆 × 𝐵𝑆 + 𝐶𝑑𝑅 × 𝐵𝑅 T-Cd=

A-Cd

(4) (5)

𝐵𝐿 + 𝐵𝑆 + 𝐵𝑅

155

where 𝐶𝑑𝐿 , 𝐶𝑑𝑆 , and 𝐶𝑑𝑅 are the concentration of Cd in leaves, stems and roots,

156

respectively. 𝐵𝐿 , 𝐵𝑆 , and 𝐵𝑅 represent ramie dry biomass in leaves, stems and roots,

157

respectively.

158 159

Besides Cd and Fe contents, the subcellular distribution of Cd in different plant tissues was measured (detection method is described in the Supporting Information). 9

ACS Paragon Plus Environment

Environmental Science & Technology

160

Oxidative

Stress

Estimation.

H2O2

and

Malondialdehyde

Page 10 of 40

(MDA)

161

concentrations were detected following the published methods (detection methods are

162

described in the Supporting Information). 37

163

Antioxidants Contents Analysis. To investigate whether plant antioxidant

164

systems under Cd-stress were modified by nanoparticles, antioxidant enzymes

165

activities and specific antioxidants contents were measured. The activities of CAT,

166

guaiacol peroxidase (POD), SOD, and the contents of GSH and GSSG in ramie

167

seedlings were assayed using commercial reagent kits purchased from Nanjing

168

JianCheng Bioengineering Institute, China (detection methods are described in the

169

Supporting Information).

170

Statistical Analysis. The results are presented as means ± standard errors of 4

171

replicates. Data were analyzed using one-way Analysis of Variance (ANOVA),

172

followed by Duncan test at a probability level of P < 0.05, performed by Statistic

173

Package for Social Science.

174

RESULTS AND DISCUSSION

175 176

Materials Characterization. The characterization of nZVI and S-nZVI are described in the Supporting Information.

177

nZVI and S-nZVI Treatments. The effects of nZVI and S-nZVI on biomass

178

production and Cd accumulation in ramie seedlings are presented in the Supporting

179

Information.

180

Effects of S-nZVI on Seedlings Growth. To investigate the effects of

181

nanoparticles on plants under metal stress, the plant growth of ramie was determined. 10

ACS Paragon Plus Environment

Page 11 of 40

Environmental Science & Technology

182

Plant dry biomass of different tissues is shown in Figure 1. The dry weight decreased

183

from 0.89 to 0.55 g/plant in leaves, from 1.38 to 1.14 g/plant in stems and from 2.02

184

to 1.53 g/plant in roots with increased S-nZVI concentrations, along with the dry

185

biomass being 0.89, 1.44 and 2.04 g/plant in the leaves, stems and roots of plants

186

cultivated in the only Cd-contaminated sediments, respectively. As shown in Figure 1,

187

100 and 500 mg/kg S-nZVI had no significant influence on plant growth under Cd

188

stress, except in the roots exposed to 500 mg/kg S-nZVI, where there is a pronounced

189

decrease of the dry weights (19.63%). Significant growth inhibition of ramie was

190

observed at 1000 mg/kg S-nZVI treatment, indicating the nanotoxicity of S-nZVI and

191

an aggravation of Cd toxicity in plants. As previously reported, high concentrations of

192

nZVI are toxic to plants.

193

concentration could be used without detrimental effects on plants, while the shoot

194

growth reduction and seeds germination inhibition were observed in ryegrass, flax and

195

barley exposed to high dosages of nZVI. Plant growth inhibition may be due to the

196

coated nZVI on the root surface thus preventing nutrients uptake into plants.

41

El-Temsah and Joner

42

found that nZVI at low

197

Cd Accumulation and Translocation in S-nZVI Treated Seedlings. To

198

investigate the effects of S-nZVI on Cd accumulation, Cd concentration in ramie

199

leaves, stems and roots were determined (Figure 2). As shown in Figure 2, Cd

200

concentration in the control plants was 2.89, 3.49 and 7.83 mg/kg DW in the leaves,

201

stems and roots, respectively. This is similar to what was observed in a previous study,

202

wherein Cd content was 0.6 mg/kg in the shoots, and 5.5 mg/kg in the roots of Acer

203

pseudoplatanus cultivated in 14.3 mg/kg Cd polluted soils. 11

ACS Paragon Plus Environment

43

The present results

Environmental Science & Technology

204

showed that Cd could be absorbed by ramie roots and translocated to the aboveground

205

parts, as reported previously. 44,45 Plants roots accumulated higher Cd when compared

206

with the stems and leaves, which may be due to the protective reaction of the

207

aboveground parts and the direct contact of roots with contaminant. Plants treated

208

with S-nZVI had significantly more Cd than the control plants. Relative to the control,

209

S-nZVI increased Cd concentration in ramie roots, stems and leaves by 16-50%,

210

29-52% and 31-73%, respectively, suggesting that enhanced Cd accumulation by

211

S-nZVI was more pronounced in plant aboveground parts. Cd concentration in ramie

212

tissues increased with increasing S-nZVI application concentrations. The highest Cd

213

content was observed in ramie treated with 1000 mg/kg S-nZVI. Because the

214

accumulation of Cd in plants not only depends on the Cd content in different tissues

215

but also relates to plant biomass, the A-Cd was calculated (Figure S5). Relative to the

216

control plants, A-Cd in ramie treated with S-nZVI increased by 14%-20%. The higher

217

Cd accumulation in ramie was not found in the treatment of 1000 mg/kg S-nZVI, but

218

was observed in the treatments of 100 and 500 mg/kg S-nZVI. Compared with the Cd

219

accumulation in the control plants, Cd uptake in the whole plants was approximately

220

1.18 and 1.20 times higher in the 100 and 500 mg/kg S-nZVI treatments respectively,

221

whereas plants treated with 1000 mg/kg S-nZVI only had a slight increase of the Cd

222

accumulation. These results were likely due to the nanotoxicity of S-nZVI to plants at

223

high concentrations, which inhibited plant growth, thus leading to a relative lower

224

amount of Cd accumulated in the 1000 mg/kg S-nZVI treated plants compared with

225

that in plants exposed to other concentrations of S-nZVI. The translocation of Cd in 12

ACS Paragon Plus Environment

Page 12 of 40

Page 13 of 40

Environmental Science & Technology

226

ramie was estimated using the translocation factor (TF), which is defined as the ratio

227

of Cd in the aerial parts to that in roots. As shown in Figure 2, all the S-nZVI

228

treatments, especially 100 mg/kg S-nZVI, increased the TF value of Cd in ramie

229

seedlings. These results showed for the first time that nZVI promoted Cd

230

accumulation and translocation in the plants. Similar to what was observed in our

231

study, nano-titanium dioxide with the concentration range from 100 to 300 mg/kg was

232

found to increase the uptake of Cd in Glycine max (L.) Merr. 46 However, different

233

from what was observed in the present study, the accumulation of Cd and Cr in wheat

234

plants exposed to iron-contained nanoparticles at concentration of 1000 mg/kg was

235

considerably decreased. 47 Gil-Díaz et al. 26 also found that nZVI decreased the uptake

236

and translocation of As in barley plants. Thus, it was speculated that the effects of

237

nanoparticles on metal accumulation in plants depended on many factors, such as

238

nanomaterials kinds, plant species, metal types and so forth.

239

Influence of S-nZVI on Cd Subcellular Distribution. In order to investigate

240

the effects of S-nZVI on Cd uptake in ramie at cell level, the subcellular distribution

241

of Cd in different plant tissues was measured. As shown in Figure 3, Cd was mainly

242

distributed in the cell wall and soluble fraction with the proportion of Cd being 48-66%

243

and 31-46%, respectively. These findings are consistent with previously published

244

studies. For example, Ramos et al. 48 demonstrated that in the leaves cells of Lactuca

245

sp., approximately 64% of Cd was in the cell wall fraction; over 70% of the total Cd

246

was found in the soluble fraction of root cells in peanut exposed to 200 mM CdCl2. 49

247

Plant cell wall acts the first barrier against entry of extracellular substances into the 13

ACS Paragon Plus Environment

Environmental Science & Technology

248

cell which is composed of polysaccharides, proteins, enzymes, and other molecules.

249

The negative charge of cell wall and S-containing proteins and other function groups,

250

such as amino group, hydroxyl and carboxyl, provide preferable conditions for Cd

251

binding and detoxification, resulting in a large proportion of Cd in cell wall. Cell

252

soluble fraction also occupied a majority percentage of Cd in ramie. This could be due

253

to the vacuolar compartmentation of Cd in the cell soluble fraction. S-nZVI treated

254

ramie seedlings showed a dose-dependent decrease in the proportion of Cd in cell

255

wall compared with the control, whereas the accumulation of Cd in soluble fraction

256

increased with increased S-nZVI concentrations. These results indicated that S-nZVI

257

enhanced the penetration of Cd into plant cells. It seems that the increased Cd

258

concentration in the soluble fraction of root cells resulted in the enhancement of Cd

259

accumulation in plants. It has been reported that iron nanoparticles could trigger cell

260

wall loosening, reduce cell wall thickness and enhance endocytosis in plants, 50 which

261

could explain the promotion of Cd entering plant cells by S-nZVI. Moreover, the

262

production of new pores and enlargement of existing pores in cell wall by

263

nanoparticles have been confirmed by recent reports, 51 and these might account for

264

the enhancement of Cd uptake and migration in plant cells exposed to S-nZVI. In

265

addition, Cd concentration in the organelle fraction increased by S-nZVI, except in

266

the 100 mg/kg S-nZVI treatment, where there was a slight decrease in the percentage

267

of Cd in the organelle fraction of ramie roots and stems. The 100 mg/kg S-nZVI

268

treatment significantly increased the T-Cd and A-Cd in ramie seedlings. However, the

269

results on plant biomass production (Figure 1) revealed that 100 mg/kg S-nZVI did 14

ACS Paragon Plus Environment

Page 14 of 40

Page 15 of 40

Environmental Science & Technology

270

not cause statistically negative impact on ramie growth and development. This is

271

probably due to the alleviation of Cd induced toxicity in plants by decreasing the

272

proportion of Cd in the cell organelle fraction at 100 mg/kg S-nZVI treatments.

273

Effects of S-nZVI on Fe Uptake. The uptake and translocation of nanoparticles

274

in plants have been demonstrated in a variety of reports, and this may provide an

275

effective means of increasing metal accumulation in plants. For a better assessment of

276

the effects of S-nZVI on Cd accumulation, Fe content in different plant tissues was

277

measured. Since Fe is an essential nutrient, some Fe was observed in the different

278

plant tissues. As shown in Figure 4, S-nZVI increased Fe content in ramie tissues and

279

this enhancement was more remarkable as S-nZVI concentrations increased. Although

280

the speciation of Fe in plant tissues was not measured, previously published studies

281

have confirmed that nZVI could be absorbed by plants. Ma et al. 28 reported that nZVI

282

could adhere on root surface and penetrate into epidermal cells of hybrid poplars. The

283

existence of nZVI in Sinapis alba and Sorghum saccharatum has been observed by

284

bright-field micrographs and DIC images, which certified the uptake of nZVI into

285

plant seedlings.

286

determined in this study and has not yet been reported. However, according to the

287

increased uptake of Fe observed in ramie seedlings exposed to S-nZVI and some

288

previous investigations, it is hypothesized that S-nZVI could be absorbed by ramie

289

seedlings. In addition, S-nZVI treatments increased Fe content in leaves by 51%-207%

290

and in stems by 27%-162%, respectively. It is expected that S-nZVI could penetrate

291

the root surface and some of them were likely to be transported to the aboveground

29

The chemical transformation of S-nZVI in ramie was not

15

ACS Paragon Plus Environment

Environmental Science & Technology

292

parts of plants. Further studies are required to determine the speciation of Fe and the

293

biotransformation of iron nanomaterials in plants.

294

nZVI has been demonstrated to immobilize Cd through absorption and surface 52

295

complexation.

In the present study, Cd accumulation and Fe uptake in ramie

296

seedlings for all the S-nZVI treatments were quite similar. Therefore, we

297

hypothesized that S-nZVI might serve as a Cd carrier to promote Cd uptake into plant

298

seedlings. As reported by Tang et al., internalization of graphene oxide (GO) occurred

299

in Microcystis aeruginosa, and the absorbed Cd on GO could easily enter this algae

300

cells, thus increasing Cd uptake into plants. 19 Besides, Cd is a non-essential metal and

301

it has no beneficial effects to plant growth and development to our knowledge. As

302

reported previously, there are no confirmed specific transport channels for Cd in

303

plants. Cd was demonstrated to get access into plants through the channels and

304

transporters for essential elements, such as calcium, manganese and potassium. 53-55

305

Specifically, Connolly et al. found that the overexpression of IRT1, a Fe transporter, in

306

Arabidopsis thaliana resulted in a higher level of Cd accumulation. 56 In the present

307

work, the addition of S-nZVI to the contaminated sediments probably will stimulate

308

the channels and transporters for Fe uptake, which sequentially give rise to the

309

promotion of Cd accumulation in the plants.

310

In the present study, results showed that Fe content in ramie seedlings increased

311

as the external S-nZVI concentrations increased. However, relative to the control,

312

1000 mg/kg S-nZVI did not show statistically significant increase of A-Cd in ramie

313

seedlings (Figure S5). Similar to previous reports in sunflower plants grown in 16

ACS Paragon Plus Environment

Page 16 of 40

Page 17 of 40

Environmental Science & Technology

314

Cd-polluted soil, Fe content was by far greater in the plants exposed to Streptomyces

315

tendae F4, while Cd accumulation in these plants only increased slightly.

316

addition, previous investigations revealed that Fe at relatively high level suppressed

317

Cd uptake in plants. 58 These results indicate that the influences of Fe on Cd uptake

318

are largely concentration-dependent. Considering the Fe content in plants was

319

changed by the external S-nZVI concentrations, the effects of S-nZVI on metal

320

accumulation are concentration-dependent.

57

In

321

However, there were no statistical differences in Fe content between the plants

322

treated with 100 mg/kg S-nZVI and the control plants. Since plants exposed to 100

323

mg/kg S-nZVI accumulated significantly more Cd in ramie compared to the control,

324

the result indicate that enhanced uptake of nanoparticles is only partly the cause of the

325

promoted Cd accumulation. Further studies are needed to explore the exact

326

mechanisms by which nanoparticles affect heavy metals uptake and translocation in

327

the plants.

328

Influence of S-nZVI on oxidative stress. To evaluate the effects of nZVI on the

329

oxidative damage in plants under Cd stress, the concentrations of MDA and H2O2 in

330

ramie seedlings were measured. MDA is a product of peroxidation processes, which

331

can react with DNA and is mutagenic to organisms. 59 MDA can be typically used as

332

the indicator of lipid peroxidation in plants, which has been certified by many

333

previous studies. 60,61 H2O2 is a representative ROS, and its excessive accumulation

334

indicates oxidative damage in plants. The overproduction of H2O2 can cause oxidative

335

injury to proteins, DNA, and lipids. 62 As shown in Figure S6, 500 and 1000 mg/kg 17

ACS Paragon Plus Environment

Environmental Science & Technology

336

S-nZVI increased MDA concentration by 30-44%, whereas the 100 mg/kg S-nZVI did

337

not significantly change the MDA content. The highest (292.22 nmol/g FW) and the

338

lowest (123.35 nmol/g FW) H2O2 content were observed in ramie seedlings treated

339

with 1000 and 100 mg/kg S-nZVI (Figure S7), respectively. The responses of MDA

340

and H2O2 to the 500 and 1000 mg/kg S-nZVI followed the same pattern: significantly

341

increased, relative to the non-nanomaterial treatment. Since MDA and H2O2 are

342

indicators of oxidative stress in plants, the results indicate that some concentrations of

343

S-nZVI aggravated the oxidative damage in ramie under Cd stress which may be due

344

to the increased uptake of Cd in these treatments. However, there were no significant

345

differences in Cd accumulation between the 500 and 1000 mg/kg S-nZVI treatments,

346

but H2O2 content significantly increased in ramie exposed to 1000 mg/kg S-nZVI and

347

a slight increase in MDA was also observed in this treatment. Therefore, we

348

hypothesized that the aggravated oxidative stress at high level of nanoparticles

349

treatments was due to the promoted Cd uptake and the nanotoxicity of nZVI. In the

350

presence of oxygen, nZVI has H2O2 production ability (reaction 1). 63 Kim et al. 50

351

demonstrated that nZVI promoted H2O2 generation and release in the plant medium.

352

The oxidative stress was observed in human bronchial epithelial cells exposed to

353

nZVI. 64 It is hypothesized that nZVI might induce oxidative damage to plants through

354

ROS generation or conversion (reaction 1-3). 59 Specifically, the increase of MDA in

355

ramie seedlings treated with higher level of S-nZVI indicate the aggravation of lipid

356

peroxidation in plants, which is probably due to the attack of nZVI on proteins and

357

polyunsaturated fatty acid residues in phospholipids.

65

18

ACS Paragon Plus Environment

Moreover, among all the

Page 18 of 40

Page 19 of 40

Environmental Science & Technology

358

treatments, H2O2 and MDA contents were the lowest in the 100 mg/kg S-nZVI

359

treatment, indicating that S-nZVI could alleviate Cd-induced oxidative stress in ramie

360

under certain conditions. The different results obtained in all the treatments suggest

361

that the effects of nanoparticles on plant oxidative stress depend on their

362

concentrations. These results are in agreements with those observed in rice seedlings,

363

wherein cerium oxide nanoparticles at 62.5 and 125 mg/L significantly reduced H2O2

364

production and 500 mg/L nanoparticles enhanced the lipid peroxidation and the

365

electrolyte leakage. 66 In addition, the aggravated oxidative damage by S-nZVI at high

366

concentrations could have resulted in the growth inhibition of ramie exposed to Cd.

367

Effects of S-nZVI on the Antioxidant Defense System. Plants have evolved

368

well-equipped strategies consisting of different enzymes, such as SOD, CAT and POD,

369

to fight against oxidative damage. SOD catalyzes the reaction of O2•- to H2O2 while

370

CAT and POD promote the conversion of H2O2 to H2O. The changes of these

371

anti-oxidative enzymes activities in ramie by S-nZVI under Cd stress are shown in

372

Figure 5A. Relative to the control, anti-oxidative enzymes activities in ramie treated

373

with 100 mg/kg S-nZVI increased. The responses of SOD and POD in ramie exposed

374

to 500 mg/kg S-nZVI followed similar pattern: significantly decreased, compared

375

with the control. The decrease was more remarkable at 1000 mg/kg S-nZVI

376

treatments. CAT activity did not change significantly by S-nZVI, except in the 1000

377

mg/kg S-nZVI treatment, wherein CAT activity decreased by 21% compared with the

378

control.

379

Non-enzymatic anti-oxidative systems composed of antioxidants also perform 19

ACS Paragon Plus Environment

Environmental Science & Technology

380

roles in the scavenging of over-produced ROS. GSH is a low molecular weight

381

antioxidant which is capable of scavenging ROS. In the ascorbate-glutathione cycle,

382

H2O2 is reduced to water accompanied with the transformation of GSH to GSSG. The

383

ratio of GSSH/GSH is the indicator of cellular redox status. In the present study, GSH

384

and GSSG were differentially affected by the concentrations of S-nZVI (Figure 5B).

385

Compared with the control, 100 mg/kg S-nZVI increased GSH content by 17%, while

386

the 500 and 1000 mg/kg S-nZVI significantly reduced its concentration in ramie

387

seedlings. On the contrary, GSSH content was the lowest in the 100 mg/kg S-nZVI

388

treatment, and it increased remarkably with increased S-nZVI concentrations. As for

389

the ratio of GSSG/GSH, the 500 and 1000 mg/kg S-nZVI increased it by 90% and

390

160%, respectively, whereas the 100 mg/kg S-nZVI did not significantly change this

391

ratio in ramie seedlings.

392

The different antioxidant responses in ramie seedlings exposed to S-nZVI

393

suggest that nZVI can modify the anti-oxidative defense systems in plants. The

394

increased activities of SOD, POD, and CAT, and the enhanced content of GSH in

395

ramie seedlings treated with 100 mg/kg S-nZVI suggest that S-nZVI could improve

396

the anti-oxidative capacity of ramie under Cd-stress at this concentration. Fe is a

397

cofactor or constituent for many enzymes, such as CAT, ascorbate peroxidase and

398

GSH peroxidase. 67 Specifically, Fe is required as the metal active site for one type of

399

SOD (Fe-SOD), and it can modify the activities of many anti-oxidative enzymes in

400

plants under metal stress. 68 For example, a study conducted by Liu et al. showed that

401

the interaction between Fe and Cd increased the activities of SOD and CAT in rice. 69 20

ACS Paragon Plus Environment

Page 20 of 40

Page 21 of 40

Environmental Science & Technology

402

It seems that the increased Fe content at 100 mg/kg S-nZVI treatment result in the

403

enhanced activities of SOD, POD and CAT in the present study. As mentioned above,

404

H2O2 content in ramie treated with 100 mg/kg S-nZVI was remarkable lower

405

compared with the control. Thus, this could have resulted from the changes in the

406

anti-oxidative enzymes activities and antioxidant contents of ramie treated with this

407

concentration of S-nZVI. These findings are consistent with previous reports. Wu et al.

408

70

409

cultivated in polybrominated diphenyl ethers contaminated soils through changing the

410

activities of SOD, CAT and POD. Tripathi et al. 71 found that in Pisum sativum (L.)

411

seedlings, silicon nanoparticles protected plants against Cr(VI) phytotoxicity via

412

up-regulating the antioxidant defense systems. Therefore, we hypothesized that low

413

level of iron nanoparticles (≤100 mg/kg) could counteract metal-induced oxidative

414

stress in plants via improving the activities of anti-oxidative enzymes and enhancing

415

the concentrations of some antioxidants. On the other hand, the increased GSSH

416

content and the raised ratio of GSSG/GSH in ramie seedlings exposed to higher level

417

of S-nZVI indicated a gradual shift to more oxidized cellular redox status in plants.

418

These changes were in accordance with the increased contents of H2O2 and MDA.

419

The over production of ROS in plants could lead to an increase in the activities of

420

anti-oxidative enzymes and the contents of antioxidants; however, the results were

421

contrary to the expectation in our experiments. The decreased activities of SOD, CAT

422

and POD and the reduced content of GSH in higher level of S-nZVI treatments

423

presumably were because of the phytotoxicity of nanoparticles. As indicated by Hong

reported that Ni/Fe nanoparticles reduced the oxidative damage to Chinese cabbage

21

ACS Paragon Plus Environment

Environmental Science & Technology

Page 22 of 40

424

et al., 72 nano ceria caused toxicity in Cucumis sativus by modifying the activities of

425

CAT, ascorbate peroxidase and dehydroascorbate reductase; silver nanoparticles

426

induced oxidative stress in Triticum aestivum L. by the accumulation of GSSG. 73 In

427

addition, the promoted Cd uptake in plants treated with 500 and 1000 mg/kg S-nZVI

428

perhaps also account for the decreased anti-oxidative enzymes activities. Overall,

429

higher level of S-nZVI blocked the anti-oxidative defense of plants under Cd-stress by

430

enhancing anti-oxidative enzymes activities and reducing antioxidant concentrations.

431

In summary, the findings of the present study demonstrate that S-nZVI promote

432

the uptake and translocation of Cd in ramie seedlings. Moreover, the oxidative stress

433

studies demonstrate that S-nZVI can modify the oxidative damage and the

434

anti-oxidative responses of plants under Cd-stress. High contents of S-nZVI

435

aggravated the oxidative damage in plants under Cd-stress resulting from the

436

promoted Cd accumulation and the phytotoxicity of nanoparticles. Although 100

437

mg/kg S-nZVI promoted Cd uptake, it alleviated Cd-induced oxidative damage in

438

plants by scavenging ROS, increasing anti-oxidative enzymes activities and

439

enhancing the antioxidant contents. These results suggest that proper concentrations

440

of nanoparticles are beneficial to promote Cd accumulation and alleviate

441

metal-induced

442

nanotechnology and phytoremediation can be regarded as a promising feasible

443

technique for the remediation of metal contaminated environment. While the

444

application of nanoparticles to facilitate soil phytoremediation is only starting to be

445

realized, further studies on the mechanisms of metal uptake and translocation, as well

toxicity

in

plants.

Therefore,

the

22

ACS Paragon Plus Environment

combined

utilization

of

Page 23 of 40

Environmental Science & Technology

446

as plant toxicity influenced by nanoparticles, are needed.

447

ASSOCIATED CONTENT

448

Supporting Information. Supplemental methods, additional results and discussion,

449

table, and figures as mentioned in the present text. This material is available free of

450

charge via the Internet at http://pubs.acs.org.

451

AUTHOR INFORMATION

452

Corresponding Author

453

* Telephone: +86-13017295768; fax: +86-731-88823701

454

E-mail: [email protected]; [email protected]

455

Notes

456

The authors declare no competing financial interest.

457

ACKNOWLEDGMENT

458

This study was financially supported by the Program for the National Natural Science

459

Foundation of China (51579098, 51408206, 51521006, 51278176, 51378190), the

460

National Program for Support of Top–Notch Young Professionals of China (2014), the

461

Program for New Century Excellent Talents in University (NCET-13-0186), the

462

Program for Changjiang Scholars and Innovative Research Team in University

463

(IRT-13R17), and Hunan Provincial Science and Technology Plan Project

464

(No.2016RS3026).

465

Reference

466

(1) Huang, D.; Liu, L.; Zeng, G.; Xu, P.; Huang, C.; Deng, L.; Wang, R.; Wan, J.

467

The effects of rice straw biochar on indigenous microbial community and enzymes 23

ACS Paragon Plus Environment

Environmental Science & Technology

468

activity in heavy metal-contaminated sediment. Chemosphere 2017, 174, 545-553.

469

(2) Seidel, H.; Löser, C.; Zehnsdorf, A.; Hoffmann, P.; Schmerold, R.

470

Bioremediation process for sediments contaminated by heavy metals: feasibility study

471

on a pilot scale. Environ. Sci. Technol. 2004, 38, (5), 1582.

472

(3) Huang, D.; Zeng, G.; Feng, C.; Hu, S.; Jiang, X.; Tang, L.; Su, F.; Zhang, Y.;

473

Zeng, W.; Liu, H. Degradation of lead-contaminated lignocellulosic waste by

474

Phanerochaete chrysosporium and the reduction of lead toxicity. Environ. Sci. Technol.

475

2008, 42, (13), 4946.

476

(4) Liang, J.; Li, X.; Yu, Z.; Zeng, G.; Luo, Y.; Jiang, L.; Yang, Z.; Qian, Y.; Wu, H.

477

Amorphous MnO2 modified biochar derived from aerobically composted swine

478

manure for adsorption of Pb (II) and Cd (II). Acs Sustain. Chem. Eng. 2017, 5, (6),

479

5049-5058.

480

(5) Jie, L.; Feng, C.; Zeng, G.; Xiang, G.; Zhong, M.; Li, X.; Xin, L.; He, X.; Fang,

481

Y. Spatial distribution and source identification of heavy metals in surface soils in a

482

typical coal mine city, Lianyuan, China. Environ. Pollut. 2017, 225, 681-690.

483

(6) Liang, J.; Zhong, M.; Zeng, G.; Chen, G.; Hua, S.; Li, X.; Yuan, Y.; Wu, H.; Gao,

484

X. Risk management for optimal land use planning integrating ecosystem services

485

values: A case study in Changsha, Middle China. Sci. Total Environ. 2016, 579,

486

1675-1682.

487

(7) Li, H. X.; Zhao, X. H.; Ma, W. F.; Wang, X. D. Phytoremediation of Complex

488

Contaminants in Sewage River Sediment by Maize. Environ. Sci. 2008, 29, (3), 709.

489

(in Chinese) 24

ACS Paragon Plus Environment

Page 24 of 40

Page 25 of 40

Environmental Science & Technology

490

(8) Huang, D.; Gong, X.; Liu, Y.; Zeng, G.; Lai, C.; Bashir, H.; Zhou, L.; Wang, D.;

491

Xu, P.; Cheng, M. Effects of calcium at toxic concentrations of cadmium in plants.

492

Planta 2017, 245, (5), 863.

493

(9) Nair, R.; Mohamed, M. S.; Gao, W.; Maekawa, T.; Yoshida, Y.; Ajayan, P. M.;

494

Kumar, D. S. Effect of carbon nanomaterials on the germination and growth of rice

495

plants. J. Nanosci. Nanotechno. 2012, 12, (3), 2212.

496

(10) Giraldo, J. P.; Landry, M. P.; Faltermeier, S. M.; Mcnicholas, T. P.; Iverson, N.

497

M.; Boghossian, A. A.; Reuel, N. F.; Hilmer, A. J.; Sen, F.; Brew, J. A. Plant

498

nanobionics approach to augment photosynthesis and biochemical sensing. Nat. Mater.

499

2014, 13, (4), 400-408.

500 501 502

(11) Lin, D.; Xing, B. Phytotoxicity of nanoparticles: inhibition of seed germination and root growth. Environ. Pollut. 2007, 150, (2), 243-250. (12) Lin, S.; Reppert, J.; Hu, Q.; Hudson, J. S.; Reid, M. L.; Ratnikova, T. A.; Rao, A.

503

M.; Luo, H.; Ke, P. C. Uptake, translocation, and transmission of carbon

504

nanomaterials in rice plants. Small 2009, 5, (10), 1128-32.

505

(13) Tang, W.; Kovalsky, P.; He, D.; Waite, T. D. Fluoride and nitrate removal from

506

brackish groundwaters by batch-mode capacitive deionization. Water Res. 2015, 84,

507

342-350.

508

(14) Tang, W. W.; Zeng, G. M.; Gong, J. L.; Liang, J.; Xu, P.; Zhang, C.; Huang, B.

509

B. Impact of humic/fulvic acid on the removal of heavy metals from aqueous

510

solutions using nanomaterials: a review. Sci. Total Environ. 2014, 468-469,

511

1014-1027. 25

ACS Paragon Plus Environment

Environmental Science & Technology

512

(15) Tang, W. W.; Zeng, G. M.; Gong, J. L.; Liu, Y.; Wang, X. Y.; Liu, Y. Y.; Liu, Z.

513

F.; Chen, L.; Zhang, X. R.; Tu, D. Z. Simultaneous adsorption of atrazine and Cu (II)

514

from wastewater by magnetic multi-walled carbon nanotube. Chem. Eng. J. 2012, s

515

211–212, (22), 470-478.

516

(16) Wang, R.; Huang, D.; Liu, Y.; Peng, Z.; Zeng, G.; Lai, C.; Xu, P.; Huang, C.;

517

Zhang, C.; Gong, X. Selective removal of BPA from aqueous solution using

518

molecularly imprinted polymers based on magnetic graphene oxide. Rsc Adv. 2016, 6,

519

(108), 106201-106210.

520

(17) Lai, C.; Wang, M.; Zeng, G.; Liu, Y.; Huang, D.; Zhang, C.; Wang, R.; Xu, P.;

521

Cheng, M.; Huang, C. Synthesis of surface molecular imprinted TiO2/graphene

522

photocatalyst and its highly efficient photocatalytic degradation of target pollutant

523

under visible light irradiation. Appl. Surf. Sci. 2016, 390, 368-376.

524

(18) Jiang, L.; Liu, Y.; Liu, S.; Zeng, G.; Hu, X.; Xi, H.; Zhi, G.; Tan, X.; Wang, L.;

525

Wu, Z. Adsorption of estrogen contaminants by graphene nanomaterials under NOM

526

preloading: Comparison with carbon nanotube, biochar and activated carbon. Environ.

527

Sci. Technol. 2017, 51, (11), 6352-6359.

528

(19) Ma, X. M.; Chen, W. Fullerene nanoparticles affect the fate and uptake of

529

trichloroethylene in phytoremediation systems. Environ Eng Sci 2010, 27, (11),

530

989-992.

531

(20) Tang, Y.; Tian, J.; Li, S.; Xue, C.; Xue, Z.; Yin, D.; Yu, S. Combined effects of

532

graphene oxide and Cd on the photosynthetic capacity and survival of Microcystis

533

aeruginosa. Sci. Total Environ. 2015, 532, 154-161. 26

ACS Paragon Plus Environment

Page 26 of 40

Page 27 of 40

534

Environmental Science & Technology

(21) Jiamjitrpanich, W.; Parkpian, P.; Polprasert, C.; Kosanlavit, R. Trinitrotoluene

535

and

Its

Metabolites

in

Shoots

and

Roots

of

Panicum

536

Nano-Phytoremediation. Int. J. Environ. Sci. Dev. 2013, 4, (1), 7-10.

maximum

in

537

(22) Gao, Y. Y.; Zhou, Q. X. Application of Nanoscale Zero Valent Iron Combined

538

with Impatiens Balsamina to Remediation of E-Waste Contaminated Soils. Adv. Mater.

539

Res. 2013, 790, 73-76.

540

(23) Liu, Q. Y.; Bei, Y. L.; Feng, Z. Removal of lead(II) from aqueous solution with

541

amino-functionalized nanoscale zero-valent iron. Cent. Eur. J. Chem. 2009, 7, (1),

542

79-82.

543

(24) Singh, R.; Misra, V.; Singh, R. P. Removal of Cr(VI) by Nanoscale Zero-valent

544

Iron (nZVI) From Soil Contaminated with Tannery Wastes. B. Environ. Contam. Tox.

545

2012, 88, (2), 210-214.

546

(25) Huang, D.; Xue, W.; Zeng, G.; Wan, J.; Chen, G.; Huang, C.; Zhang, C.; Cheng,

547

M.; Xu, P. Immobilization of Cd in river sediments by sodium alginate modified

548

nanoscale zero-valent iron: Impact on enzyme activities and microbial community

549

diversity. Water Res. 2016, 106, 15–25.

550

(26) Gildíaz, M.; Diezpascual, S.; González, A.; Alonso, J.; Rodrí guezvaldés, E.;

551

Gallego, J. R.; Lobo, M. C. A nanoremediation strategy for the recovery of an

552

As-polluted soil. Chemosphere 2016, 149, 137-145.

553

(27) Wang, Y.; Fang, Z.; Kang, Y.; Tsang, E. P. Immobilization and phytotoxicity of

554

chromium in contaminated soil remediated by CMC-stabilized nZVI. J. Hazard.

555

Mater. 2014, 275, (2), 230-237. 27

ACS Paragon Plus Environment

Environmental Science & Technology

556 557

(28) Ma, X.; Gurung, A.; Deng, Y. Phytotoxicity and uptake of nanoscale zero-valent iron (nZVI) by two plant species. Sci. Total Environ. 2013, 443, (3), 844-849.

558

(29) Libralato, G.; Costa, D. A.; Zanella, M.; Sabbioni, E.; Mičetić, I.; Manodori, L.;

559

Pigozzo, A.; Manenti, S.; Groppi, F.; Volpi, G. A. Phytotoxicity of ionic, micro- and

560

nano-sized iron in three plant species. Ecotox. Environ. Safe. 2015, 123, 81-88.

561

(30) Zhang, Y.; Li, Y.; Dai, C.; Zhou, X.; Zhang, W. Sequestration of Cd(II) with

562

nanoscale zero-valent iron (nZVI): Characterization and test in a two-stage system.

563

Chem. Eng. J. 2014, 244, (244), 218-226.

564

(31) A, Š.; Eltemsah, Y. S.; Joner, E. J.; M, Č. Oxidative stress induced in

565

microorganisms by zero-valent iron nanoparticles. Microbes Environ. 2011, 26, (4),

566

271-281.

567

(32) Huang, D.; Hu, C.; Zeng, G.; Min, C.; Xu, P.; Gong, X.; Wang, R.; Xue, W.

568

Combination of Fenton processes and biotreatment for wastewater treatment and soil

569

remediation. Sci. Total Environ. 2017, 574, 1599-1610.

570

(33) Hong, J.; Peralta-Videa, J. R.; Rico, C.; Sahi, S.; Viveros, M. N.; Bartonjo, J.;

571

Zhao, L.; Gardea-Torresdey, J. L. Evidence of translocation and physiological impacts

572

of foliar applied CeO2 nanoparticles on cucumber (Cucumis sativus) plants. Environ.

573

Sci. Technol. 2014, 48, (8), 4376-85.

574

(34) Wang, X.; Dong, M. Y.; Liu, L.; Liu, Y.; Jin, Z. H.; Li, T. L. Study on

575

Cytotoxicity of Nanoscale Zero Valent Iron Particles. Mater. Sci. Forum 2011, 694,

576

224-228.

577

(35) Chaithawiwat, K.; Vangnai, A.; Mcevoy, J. M.; Pruess, B.; Krajangpan, S.; 28

ACS Paragon Plus Environment

Page 28 of 40

Page 29 of 40

Environmental Science & Technology

578

Khan, E. Role of oxidative stress in inactivation of Escherichia coli BW25113 by

579

nanoscale zero-valent iron. Sci. Total Environ. 2016, 565, 857-862.

580

(36) Alidokht, L.; Khataee, A. R.; Reyhanitabar, A.; Oustan, S. Reductive removal of

581

Cr(VI) by starch-stabilized Fe0 nanoparticles in aqueous solution. Desalination 2011,

582

270, (1–3), 105-110.

583

(37) Gong, X.; Liu, Y.; Huang, D.; Zeng, G.; Liu, S.; Tang, H.; Zhou, L.; Hu, X.;

584

Zhou, Y.; Tan, X. Effects of exogenous calcium and spermidine on cadmium stress

585

moderation and metal accumulation in Boehmeria nivea (L.) Gaudich. Environ. Sci.

586

Pollut. R. 2016, 23, (9), 8699-8708.

587

(38) Huang, D.; Chen, G.; Zeng, G.; Xu, P.; Yan, M.; Lai, C.; Zhang, C.; Li, N.;

588

Cheng, M.; He, X. Synthesis and Application of Modified Zero-Valent Iron

589

Nanoparticles for Removal of Hexavalent Chromium from Wastewater. Water Air Soil

590

Poll. 2015, 226, (11), 1-14.

591

(39) Ji, B. X.; Mahmood, Q.; Min, Y. The potential of Sedum alfredii Hance for the

592

biosorption of some metals from synthetic wastewater. Desalination 2011, 267, (2–3),

593

154-159.

594

(40) Yang, X.; Baligar, V. C.; Martens, D. C.; Clark, R. B. Cadmium effects on influx

595

and transport of mineral nutrients in plant species. J. Plant Nutr. 2008, 19, (3-4),

596

291-314.

597

(41) Wang, J.; Fang, Z.; Cheng, W.; Yan, X.; Tsang, P. E.; Zhao, D. Higher

598

concentrations of nanoscale zero-valent iron (nZVI) in soil induced rice chlorosis due

599

to inhibited active iron transportation. Environ. Pollut. 2016, 210, 338-345. 29

ACS Paragon Plus Environment

Environmental Science & Technology

600

(42) El‐Temsah, Y.; Joner, E. Impact of Fe and Ag nanoparticles on seed germination

601

and differences in bioavailability during exposure in aqueous suspension and soil.

602

Environ. Toxicol. 2012, 27, (1), 42-50.

603

(43) Quezada-Hinojosa, R.; Föllmi, K. B.; Gillet, F.; Matera, V. Cadmium

604

accumulation in six common plant species associated with soils containing high

605

geogenic cadmium concentrations at Le Gurnigel, Swiss Jura Mountains. Catena

606

2015, 124, (124), 85-96.

607

(44) Liu, Y.; Wang, X.; Zeng, G.; Qu, D.; Gu, J.; Zhou, M.; Chai, L.

608

Cadmium-induced oxidative stress and response of the ascorbate–glutathione cycle in

609

Bechmeria nivea (L.) Gaud. Chemosphere 2007, 69, (1), 99-107.

610

(45) Gong, X.; Liu, Y.; Huang, D.; Zeng, G.; Liu, S.; Hui, T.; Lu, Z.; Xi, H.; Zhou, Y.;

611

Tan, X. Effects of exogenous calcium and spermidine on cadmium stress moderation

612

and metal accumulation in Boehmeria nivea (L.) Gaudich. Environ. Sci. Pollut. R.

613

2016, 23, (9), 8699-8708.

614

(46) Singh, J.; Lee, B. K. Influence of nano-TiO2 particles on the bioaccumulation of

615

Cd in soybean plants (Glycine max): A possible mechanism for the removal of Cd

616

from the contaminated soil. J. Environ. Manage. 2016, 170, 88-96.

617

(47) Lópezluna, J.; Silvasilva, M. J.; Martinezvargas, S.; Mijangosricardez, O. F.;

618

Gonzálezchávez, M. C.; Solísdomínguez, F. A.; Cuevasdíaz, M. C. Magnetite

619

nanoparticle (NP) uptake by wheat plants and its effect on cadmium and chromium

620

toxicological behavior. Sci. Total Environ. 2016, 565, 941-950.

621

(48) Ramos, I.; Esteban, E.; Lucena, J. J.; Gárate, A. N. Cadmium uptake and 30

ACS Paragon Plus Environment

Page 30 of 40

Page 31 of 40

Environmental Science & Technology

622

subcellular distribution in plants of Lactuca sp. Cd–Mn interaction. Plant Sci. 2002,

623

162, (5), 761-767.

624

(49) Su, Y.; Liu, J.; Lu, Z.; Wang, X.; Zhang, Z.; Shi, G. Effects of iron deficiency on

625

subcellular distribution and chemical forms of cadmium in peanut roots in relation to

626

its translocation. Environ. Exp. Bot. 2014, 97, (1), 40-48.

627

(50) Kim, J. H.; Lee, Y.; Kim, E. J.; Gu, S.; Sohn, E. J.; Seo, Y. S.; An, H. J.; Chang,

628

Y. S. Exposure of iron nanoparticles to Arabidopsis thaliana enhances root elongation

629

by triggering cell wall loosening. Environ. Sci. Technol. 2014, 48, (6), 3477-85.

630 631

(51) Nair, R.; Varghese, S. H.; Nair, B. G.; Maekawa, T.; Yoshida, Y.; Kumar, D. S. Nanoparticulate material delivery to plants. Plant Sci. 2010, 179, (3), 154-163.

632

(52) Zhang, Y.; Li, Y.; Dai, C.; Zhou, X.; Zhang, W. Sequestration of Cd(II) with

633

nanoscale zero-valent iron (nZVI): Characterization and test in a two-stage system.

634

Chem. Eng. J. 2014, 244, (244), 218–226.

635

(53) Pilonsmits, E. Increased accumulation of cadmium and lead under Ca and Fe

636

deficiency in Typha latifolia: A study of two pore channel (TPC1) gene responses.

637

Environ. Exp. Bot. 2015, 115, (6), 38-48.

638

(54) Sasaki, A.; Yamaji, N.; Yokosho, K.; Ma, J. F. Nramp5 Is a Major Transporter

639

Responsible for Manganese and Cadmium Uptake in Rice. Plant cell 2012, 24, (5),

640

2155-67.

641

(55) Mleczek, M.; Kozlowska, M.; Kaczmarek, Z.; Chadzinikolau, T.; Golinski, P.

642

Influence of Ca/Mg ratio on phytoextraction properties of Salix viminalis I. The

643

effectiveness of Cd, Cu, Pb, and Zn bioaccumulation and plant growth. Int. J. 31

ACS Paragon Plus Environment

Environmental Science & Technology

644

Phytoremediat. 2012, 14, (1), 75-88.

645

(56) Connolly, E. L.; Fett, J. P.; Guerinot, M. L. Expression of the IRT1 metal

646

transporter is controlled by metals at the levels of transcript and protein accumulation.

647

Plant Cell 2002, 14, (6), 1347-57.

648

(57) Dimkpa, C. O.; Merten, D.; Svato, A.; Büchel, G.; Kothe, E. Siderophores

649

mediate reduced and increased uptake of cadmium by Streptomyces tendae F4 and

650

sunflower (Helianthus annuus), respectively. J. Appl. Microbiol. 2009, 107, (5),

651

1687-1696.

652

(58) Shao, G.; Chen, M.; Wang, W.; Mou, R.; Zhang, G. Iron nutrition affects

653

cadmium accumulation and toxicity in rice plants. Plant Growth Regul. 2007, 53, (1),

654

33-42.

655 656 657 658

(59) Valko, M.; Morris, H.; Cronin, M. T. Metals, toxicity and oxidative stress. Curr. Med. Chem. 2005, 12, (10), 1161-1208. (60) Gaweł, S.; Wardas, M.; Niedworok, E.; Wardas, P. Malondialdehyde (MDA) as a lipid peroxidation marker. Wiadomosci Lekarskie 2004, 57, (9-10), 453-458.

659

(61) Davey, M. W.; Stals, E.; Panis, B.; Keulemans, J.; Swennen, R. L.

660

High-throughput determination of malondialdehyde in plant tissues. Anal. Biochem.

661

2005, 347, (2), 201-207.

662 663

(62) Apel, K.; Hirt, H. Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu. Rev. Plant Biol. 2004, 55, (1), 373-399.

664

(63) Zou, Y.; Wang, X.; Khan, A.; Wang, P.; Liu, Y. H.; Alsaedi, A.; Hayat, T.; Wang,

665

X. Environmental Remediation and Application of Nanoscale Zero-Valent Iron and Its 32

ACS Paragon Plus Environment

Page 32 of 40

Page 33 of 40

Environmental Science & Technology

666

Composites for the Removal of Heavy Metal Ions: A Review. Environ. Sci. Technol.

667

2016, 50, (14), 7290-7304.

668

(64) Keenan, C. R.; Goth-Goldstein, R.; Lucas, D.; Sedlak, D. L. Oxidative stress

669

induced by zero-valent iron nanoparticles and Fe(II) in human bronchial epithelial

670

cells. Environ. Sci. Technol. 2009, 43, (12), 4555-4560.

671

(65) Dwivedi, A. D.; Ma, L. Q. Biocatalytic Synthesis Pathways, Transformation,

672

and Toxicity of Nanoparticles in the Environment. Crit. Rev. Env. Sci. Tec. 2014, 44,

673

(15), 1679-1739.

674

(66) Rico, C. M.; Hong, J.; Morales, M. I.; Zhao, L.; Barrios, A. C.; Zhang, J. Y.;

675

Peralta-Videa, J. R.; Gardea-Torresdey, J. L. Effect of cerium oxide nanoparticles on

676

rice: a study involving the antioxidant defense system and in vivo fluorescence

677

imaging. Environ. Sci. Technol. 2013, 47, (11), 5635-42.

678

(67) Sharma, S. S.; Kaul, S.; Metwally, A.; Goyal, K. C.; Finkemeier, I.; Dietz, K. J.

679

Cadmium toxicity to barley (Hordeum vulgare) as affected by varying Fe nutritional

680

status. Plant Sci. 2004, 166, (5), 1287-1295.

681

(68) Mohasseli, V.; Khoshgoftarmanesh, A. H.; Shariatmadari, H. The Effect of Air

682

Pollution on Leaf Iron (Fe) Concentration and Activity of Fe-Dependent Antioxidant

683

Enzymes in Maple. Water Air Soil Poll. 2016, 227, (1), 12-22.

684

(69) Liu, H.; Zhang, C.; Wang, J.; Zhou, C.; Feng, H.; Mahajan, M. D.; Han, X.

685

Influence and interaction of iron and cadmium on photosynthesis and antioxidative

686

enzymes in two rice cultivars. Chemosphere 2017, 171, 240-247.

687

(70) Wu, J.; Xie, Y.; Fang, Z.; Cheng, W.; Tsang, P. E. Effects of Ni/Fe bimetallic 33

ACS Paragon Plus Environment

Environmental Science & Technology

688

nanoparticles on phytotoxicity and translocation of polybrominated diphenyl ethers in

689

contaminated soil. Chemosphere 2016, 162, 235-242.

690

(71) Tripathi, D. K.; Singh, V. P.; Prasad, S. M.; Chauhan, D. K.; Dubey, N. K.

691

Silicon nanoparticles (SiNp) alleviate chromium (VI) phytotoxicity in Pisum sativum

692

(L.) seedlings. Plant Physiol. Bioch. 2015, 96, 189-198.

693

(72) Hong, J.; Peraltavidea, J. R.; Rico, C.; Sahi, S.; Viveros, M. N.; Bartonjo, J.;

694

Zhao, L.; Gardeatorresdey, J. L. Evidence of translocation and physiological impacts

695

of foliar applied CeO2 nanoparticles on cucumber (Cucumis sativus) plants. Environ.

696

Sci. Technol. 2014, 48, (8), 4376-85.

697

(73) Dimkpa, C. O.; Mclean, J. E.; Martineau, N.; Britt, D. W.; Haverkamp, R.;

698

Anderson, A. J. Silver nanoparticles disrupt wheat (Triticum aestivum L.) growth in a

699

sand matrix. Environ. Sci. Technol. 2013, 47, (2), 1082-1090

34

ACS Paragon Plus Environment

Page 34 of 40

Page 35 of 40

Environmental Science & Technology

700 701

Figure 1. Dry biomass of the leaves, stems and roots of ramie seedlings treated with

702

different concentrations of S-nZVI. Control, S-nZVI1, S-nZVI5 and S-nZVI10 denote

703

0, 100, 500 and 1000 mg/kg S-nZVI treatments. Data are means ± standard error of

704

four replicates. Different letters (a, b, c) above the error bars indicate significant

705

differences among different treatments (p < 0.05) using one-way ANOVA followed by

706

Duncan test.

707

35

ACS Paragon Plus Environment

Environmental Science & Technology

708 709

Figure 2. Cd accumulation and translocation in ramie seedlings treated with different

710

concentrations of S-nZVI. Control, S-nZVI1, S-nZVI5 and S-nZVI10 denote 0, 100,

711

500 and 1000 mg/kg S-nZVI treatments. Data are means ± standard error of four

712

replicates. Different letters (a, b, c) above the error bars indicate significant

713

differences among different treatments (p < 0.05) using one-way ANOVA followed by

714

Duncan test.

36

ACS Paragon Plus Environment

Page 36 of 40

Page 37 of 40

Environmental Science & Technology

715 716

Figure 3. Subcellular distribution of Cd in the leaves, stems and roots of ramie

717

seedlings treated with different concentrations of S-nZVI. Control, S-nZVI1, S-nZVI5

718

and S-nZVI10 denote 0, 100, 500 and 1000 mg/kg S-nZVI treatments.

37

ACS Paragon Plus Environment

Environmental Science & Technology

719 720

Figure 4. Fe uptake in the leaves, stems and roots of ramie seedlings treated with

721

different concentrations of S-nZVI. Control, S-nZVI1, S-nZVI5 and S-nZVI10 denote

722

0, 100, 500 and 1000 mg/kg S-nZVI treatments. Data are means ± standard error of

723

four replicates. Different letters (a, b, c) above the error bars indicate significant

724

differences in the accumulation of Fe in leaves, stems and roots, respectively, among

725

the treatments (p < 0.05).

38

ACS Paragon Plus Environment

Page 38 of 40

Page 39 of 40

Environmental Science & Technology

726 727

728 729

Figure 5. Antioxidant substances concentrations in ramie seedlings treated with

730

different concentrations of S-nZVI. A corresponds to the activities of CAT, SOD, and

731

POD in ramie seedlings. B corresponds to the contents of GSSH and GSH, and the 39

ACS Paragon Plus Environment

Environmental Science & Technology

732

ratio of GSSG/GSH in ramie seedlings. Control, S-nZVI1, S-nZVI5 and S-nZVI10

733

denote 0, 100, 500 and 1000 mg/kg S-nZVI treatments. Data are means ± standard

734

error of four replicates. Different letters (a, b, c) above the error bars indicate

735

significant differences among different treatments (p < 0.05) using one-way ANOVA

736

followed by Duncan test.

40

ACS Paragon Plus Environment

Page 40 of 40