Steroids From Solanum jasminoides - Journal of Natural Products

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~ I A D HJAIS, L - -1.K. R.ITHORE*and 5.C. J I I S D e p a r t m e n t qf Botany, L-nizersity of Rqjiisthan, Jilipur, Indzo t h e characteristic colors developed 118). Five spots were labelled (-1 t o E ) in order of decreasing R f value. I n a parallel chromatogram spots C, and E in the flowers and roots corresponded t o sitosterol, cho1es;erol and diosgenin respectiwly, but in t h e aerial p a r t s spots D and E coincided with sitosterol and cholesterol. Bands C , D , and E were separated by preparative tlc on air-dried silica gel G layers (wet thickness 500 mp'i by using two-fold development in hexane-ethyl acetate t3:11 and spraying with anisaldehyde reagent. Bands coinciding m-ith sitosterol, cholesterol, and diosgenin were marked, scraped from unsprayed plates, eluted with chloroform, tested by tlc for their purity, eluted, and later crystallized (19). Spots d and B in t h e flow-ers and roots, and spots -1,B, and C in t h e aerial p a r t s could not be identified due t o insrifficient quantit?-. F o r the isolated dioseenin we determined: mp 203-201", mnip undepressed, acetate nip 191-195', :a:25D-129', and t h e i r spectrum (characteristic peaks a t 984, 922, 901 and 806 em-'). Sitosterol and cholesterol were identified on t h e basis of t h e LieherniannBurchard t e s t , tlc in various solvents, color reactions, mp, and i r spectral studies. Diosgenin was estimated quantitative1)- in t h e isolates of flowers and roots.

The occurrence of dioagenin has been reported in various Solaiiiim species (1-14). but it has not been reported from s. jasminoities Past. The presence of solasonine. solamargine, and the hydrolytic product solasodine ha.; been investigated in different plant parts of this species (15IG). In continuation of earlier findings. n-e report here the isolation and identification of steroids from flower roots: and aerial parts of S. jasnii-

n,

iioides.

EXPERINESThL PL.LST~IITERI.~L.--.S.jiisniinoides P a s t . plants were collected during October 1 9 i i a t the Botanical Garden of Maharaja's College, Jaipur: India. -1voucher specimen of this collection has been deposited with t h e herbarium of t h e Botany Department, Cniversit)- of Rajasthan, Jaipur, India.

ESTR-ICTIOSOF STEROIDS.-Dried and powdered flom-ers, roots, and aerial p a r t s without flowers 110 g each) of S j m n i i n o i d e s were separately defatted in succession with 100 ml of petroleum e t h e r (24 h r ) and benzene 116 h r ) . T h e defatted plant samples, obtained after filtration, were air-dried, repon-dered and separate1)- refluxed for 4 h r with 20 ml g of 5s HC1 in 7 0 7 ethanol. Each hydrolyzate solution (approximately 200 m l ) obtained after filtration was repeatedly estracted with e t h y l acetate i l i ) for complete extraction of sapogenins. 1-arioils fractions were pooled, dried in i'ilcuo and taken up in chloroform for further analyses.

ISOLITIOS .LSD IDESTIFIC.ITIOS

COLORIMETRIC ESTIXITIOS OF DIOSGESIS .Diosgenin was quantitatively estimated by t h e method of Sanchez et ill. (20) after tlc on silica gel G with n-hesane-ethyl acetate (3:l). -1bsorbances measured with a spectrophotometer (Carl Zeiss, ,Jena D D R , T-SU-2 P) set a t 405 nni against a blank m-ere referred t o a calibration curve which followed t h e Beer's law. T h e concentrations of t h e two extracts (flowers and roots) were determined by taking t h e mean values of 15 replicates.

OF S T E R O I D S .

RESULTS Diosgenin and tn-o sterols (sitosterol and cholesterol) not knon-n to occur in the plant species under investigation have been isolated and identified both from flon-ers and roots. but the other aerial parte n-ere found t o lack diosgenin. Higher diosgenin content \\-as pre.sent in roots (0.33% dry weight ) as compared to flon-ere (0.1SX ). Since the fruit

-Each crude steroid extract was examined b>- two-dimensional tlc on air-dried silica gel G iwet thickness 250 nip) using dichloromethane-methanol-formamide(93:G:l'i i n t h e first direction and cyclohesane-ethyl acetate-water (Ci00:400:1) in t h e second direction 118). T h e steroidal compounds were located by spraying with 5 0 5 sulfuric acid and, also separate1)- TT-ith anisaldehyde reagent 10.5ml of anisaldehyde in 50 ml of glacial acetic acid and 1 nil of concentrated sulfuric acid) and then heating at 100" until *Ilepartnient of Horticnltiire, Univer.sit>of Udaipiir, Udaipiir. India.

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setting is very 10~1-in the plant under cultivation, the occurrence of diosgenin in the flowers is noteworthy. ACKNOWLEDGMESTS We wish t o thank the University Grants Commission, New Delhi, India, for financial assistance granted to one of u s (SCJ) and t o thank Dr. (hliss) Pushpa Khanna for valuable suggestions. Recekled 98 4 u g z i s t 1978. 1.

2. 3.

4. 5.

6.

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[VOL.

42, so. 4

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