Structural Analysis of 2D-Gel-Separated Glycoproteins from Human Cerebrospinal Fluid by Tandem High-Resolution Mass Spectrometry Kristina Håkansson,† Mark R. Emmett,†,‡ Alan G. Marshall,†,‡ Pia Davidsson,§ and Carol L. Nilsson*,| Ion Cyclotron Resonance Program, National High Magnetic Field Laboratory, Florida State University, 1800 East Paul Dirac Drive, Tallahassee, Florida 32310-3706, Department of Chemistry and Biochemistry, Florida State University, Tallahassee, Florida 32306-4390, Institute of Clinical Neuroscience, Sahlgrenska University Hospital, Go¨teborg University, SE-431 80 Mo¨lndal, Sweden, and Institute of Medical Biochemistry, Go¨teborg University, Box 440, SE-405 30 Go¨teborg, Sweden Received April 9, 2003
The feasibility of global glycoprotein analysis by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry and infrared multiphoton dissociation (IRMPD) tandem mass spectrometry is demonstrated. Combined 2D gel glycoprotein separation and visualization, in-gel digestion, and accurate (
