Structural Analysis of the Glycosylated Intact HIV-1 gp120–b12

Jan 19, 2017 - ... Cheng Cheng , Guillaume B.E. Stewart-Jones , Gwo-Yu Chuang ... Diana G. Scorpio , Lawrence Shapiro , Adrian B. McDermott , John R...
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Structural Analysis of Glycosylated Intact HIV-1 gp120-b12 Antibody Complex using Hydroxyl Radical Protein Footprinting Xiaoyan Li, Oliver C. Grant, Keigo Ito, Aaron Wallace, Shixia Wang, Peng Zhao, Lance Wells, Shan Lu, Robert J. Woods, and Joshua S Sharp Biochemistry, Just Accepted Manuscript • DOI: 10.1021/acs.biochem.6b00888 • Publication Date (Web): 19 Jan 2017 Downloaded from http://pubs.acs.org on January 26, 2017

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Biochemistry

Structural Analysis of Glycosylated Intact HIV-1 gp120-b12 Antibody Complex using Hydroxyl Radical Protein Footprinting Xiaoyan Li1†, Oliver C. Grant1†, Keigo Ito1, Aaron Wallace2, Shixia Wang2, Peng Zhao1, Lance Wells1, Shan Lu2, Robert J. Woods1, and Joshua S. Sharp3* 1

Complex Carbohydrate Research Center, University of Georgia, Athens, GA, 30602,

USA 2

Department of Medicine, University of Massachusetts Medical School, Worcester, MA,

01605, USA 3

Department of BioMolecular Sciences, University of Mississippi, University, MS 38677



Authors contributed equally to the manuscript

*Correspondence to: Joshua S. Sharp; email: [email protected] Department of BioMolecular Sciences P.O. Box 1848, University, MS 38677-1848 Phone: (662) 915-1758 Fax: (662) 915-5638

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ABSTRACT Glycoprotein gp120 is a surface antigen and virulence factor of the human immunodeficiency virus-1 (HIV-1). Broadly neutralizing antibodies (bNAbs) that are reactive to gp120 from a variety of HIV isolates offer hope for the development of broadly effective immunogens for vaccination purposes, if the interactions between gp120 and bNAbs can be understood. From a structural perspective, gp120 is a particularly difficult system due to its size, the presence of multiple flexible regions, and the large amount of glycosylation, all of which are important in gp120-bNAb interactions. Here, the interaction of full-length, glycosylated gp120 with the bNAb b12 is probed using high resolution hydroxyl radical protein footprinting (HR-HRPF) by fast photochemical oxidation of proteins (FPOP). HR-HRPF allows for the measurement in changes of average solvent accessible surface area of multiple amino acids without the need for measures that might alter the protein conformation, such as mutagenesis. HRHRPF of the gp120-b12 complex coupled with computational modeling shows a novel extensive interaction of the V1/V2 domain, probably with the light chain of b12. Our data also reveal HR-HRPF protection in the C3 domain due to interaction of the N330 glycan with the b12 light chain. In addition to providing information on the interactions of full-length, glycosylated gp120 with b12, this work serves as a template for the structural interrogation of full-length glycosylated gp120 with other bNAbs to better characterize the interactions that drive the broad specificity of the bNAb.

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The human immunodeficiency virus-1 (HIV-1) gp120 envelope glycoprotein is the major target for neutralizing antibodies

1, 2

. The gp120 molecule consists of a polypeptide core

of roughly 60 kDa. Extensive modification by N-linked glycosylation increases the molecular weight of the molecule to ~120 kDa 3. The amino acid sequence of gp120 is composed of five conserved regions (C1-C5) and five variable regions (V1-V5), many of which are highly flexible. The majority of antibodies raised against gp120 have very narrow ranges of effectiveness and are eventually evaded by the virus. However, a subset of raised antibodies have been found to be effective against a broader array of isolates. The development of a vaccine immunogen which elicits these broadly neutralizing antibodies (bNAbs) and confers protective immunity still remains a challenge. Improved knowledge of the Env structure and what constitutes a full neutralization epitope will help rational immunogen design in order to elicit potent bNAbs. However, gp120 is a very challenging molecule for structural biology. The extensive glycosylation, diversity of isoforms and broad conformational flexibility of gp120 pose formidable barriers for crystallization. To surmount these difficulties and construct a crystal structure for gp120, sources of likely conformational heterogeneity such as N-linked carbohydrates, flexible or mobile N and C termini, and variable internal loops (like V1/V2, and/or V3) are often reduced or eliminated, and ligands such as CD4 are used to restrict conformational mobility as well as to alter the crystallization surface 4-12. These stabilized structures give valuable information at high resolution, but at the cost of eliminating regions which have been shown to be important for many gp120-antibody interactions 13. The first broadly neutralizing human monoclonal antibody (mAb) b12 was isolated from clade B infected patients and binds to gp120 at and near its CD4 binding site (CD4bs) 7, 10,

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. Binding of b12 to the surface of gp120 blocks attachment of CD4 and thus prevents

the entry of HIV-1 into a target cell

7, 10

, Therefore, gp120 appears to present the b12

epitope in conjunction with several other weakly neutralizing and overlapping epitopes. However, while several other CD4bs antibodies have been discovered since with greater potency and breadth than b12, b12 remains a valuable model for anti-CD4bs bNAbs due to its history of experimental study

15-17

. A crystal structure of b12 in complex with a

truncated, deglycosylated, and mutationally stabilized gp120 core (PDBID 2NY7) has revealed that the contacts between b12 and gp120 are centered around the CD4-binding loop spanning residues 364–373, but involves many other residues

10

. The truncated,

deglycosylated, and mutationally stabilized gp120 core has important differences from its mature counterpart, including a fully truncated V1/V2 domain. It was found that the removal of V1/V2 loops significantly decreases the binding of b12 to gp120

17

. The

removal of a single N-linked glycosylation site at the V3 loop increased the neutralization sensitivity by CD4bs-antibodies

18

. Because of their absence in the crystal structure of

gp120 core in complex with b12, it remains unclear how the V1/V2 and V3 loops interact with b12. The characterization of the contact sites between mature gp120 and b12 will provide a better understanding of the specific broadly neutralization activity of b12 against gp120. As there is no crystal structure available of an intact, glycosylated gp120 in complex with b12, molecular modeling has been used to predict the interface between b12 and gp120 core at the truncated area by using available crystal structures as the basis for homology modeling

5, 7, 19

. However, such molecular modeling has limitations in

accuracy and reliability, and should ideally be coupled with experimental testing and constraints 20.

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In this study, we used high resolution hydroxyl radical protein footprinting (HR-HRPF) by fast photochemical oxidation of proteins (FPOP) coupled with mass spectrometry (MS) to characterize the binding interface between mature HIV-1 JR-FL gp120 and b12. Hydroxyl radical protein footprinting is a relatively new method for probing changes in the topography of a protein. The measured rate of the reaction of hydroxyl radicals at any particular amino acid side chain depends primarily upon two factors: the chemical reactivity of the side chain (which is invariant between conformations) and the accessibility of the side chain to the radical. Therefore, by monitoring changes in the HRHRPF reactivity, we can determine changes in average solvent accessibility due to changes in conformation and/or dynamics or direct shielding by protein-protein or protein-ligand binding. MS-based hydroxyl radical protein footprinting has shown great promise in the measurement of protein conformational changes, protein-protein interactions and protein-ligand binding events including FPOP

28

21-27

. HR-HRPF by certain techniques,

and electron accelerator radiolysis

29

, stably modifies solvent

accessible amino acid side chains without deforming the protein structure during the timescale of modification , allowing for heavy surface labeling of the native structure. In order to measure changes in HR-HRPF reactivity, the stable modifications to the protein side chains are analyzed by liquid chromatography-mass spectrometry (LC-MS), relatively quantifying modified and unmodified peptides, and the MS signal of the oxidized version(s) of each peptide is compared to the MS signal of the unoxidized version of the same peptide to quantify oxidation at the peptide level. By measuring the rate of reaction of each amino acid side chain of a protein under two different structural conditions (in this case, b12-bound vs. free gp120), relative changes in the accessibility

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of each amino acid can be measured

25

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. Previous work from our group has shown that,

using ETD-based methods for quantifying multiple adjacent sites of isomeric oxidation products, we can accurately quantify changes in the hydroxyl radical footprint down to single amino acid spatial resolution

30, 31

spatial resolution and more accuracy.

, providing structural information with higher This method offers an important alternative

method for characterizing protein-protein and protein-ligand interactions in cases in which it is not possible to determine high resolution three-dimensional structures of complexes due to heterogeneity, flexibility, and/or size of the target(s). A glycosylated, full-length homology model of JR-FL gp120 was constructed and a 500 ns molecular dynamics simulation was performed. The resulting simulation was aligned to the available structure of gp120 HXBc2 in complex with b12 allowing an estimation of the contact area between b12 and glycosylated JR-FL. This provided a structure-based rationalization of the experimentally determined protection sites as well as detailed insights into the regions of JR-FL affected by complexation with b12.

Materials and Methods Materials and Reagents. Hydrogen peroxide (30%) was purchased from J.T. Baker (Phillipsburg, NJ). Dithiothreitol (DTT), HPLC-grade acetonitrile were purchased from Fisher Scientific (Fair Lawn, NJ). Methionine amide was purchased from Bachem (Torrace, CA, USA). Catalase, adenine, formic acid, L-glutamine and phosphate buffer saline (PBS) were obtained from the Sigma-Aldrich Corporation (St. Louis, MO). Sequencing-grade modified trypsin and Glu-C were purchased from Promega Corporation (Madison, WI, USA). PNGase F (500,000 U/mL) was purchased from New England Biolabs (Ipswich, MA). All reagents were used without further purification.

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Purified water (18 MΩ) was obtained from an in-house Milli-Q Synthesis system (Millipore, Billerica, MA). Construction, Expression and Purification of Gp120 and Human mAbs IgG1 b12. Recombinant HIV-1 gp120 protein vaccine was produced from gp120-JR-FL DNA vaccine transfected 293F cells.

Codon optimized gp120-JR-FL DNA vaccine was

constructed into the pJW4303 DNA vaccine vector as previously reported 32. At 72 hours after the transient transfection of 293F cells, the culture supernatant was harvested and the secreted gp120 protein from were purified using a lectin column. The purified gp120 protein were verified and analyzed by SDS-PAGE and Western blot analysis (data not shown).

The monoclonal antibody (mAb) IgG1 b12, specific for gp120 CD4 binding

site (CD4bs), was purchased from Polymun Scientific (Austria). HR-HRPF by FPOP. 6 µL gp120 JR-FL (25 µM) was incubated with 6 µL PBS either with or without 25 µM b12 at 37oC for 1 h. HR-HRPF by FPOP was then performed in triplicate as previously described . Briefly, 4 µL glutamine (100 mM) and 2 µL adenine (200 µg/mL) were mixed with the 12 µL gp120 alone or gp120-b12 reaction solution. Adenine was added as a radical dosimeter to monitor the available radical dose in each sample. 2 µL of 3% hydrogen peroxide (1 M, freshly prepared) was added into the mixture immediately prior to irradiation. The FPOP labeling of the total volume of 20 µL mixture was immediately performed The KrF excimer laser power (GAM Laser Inc., Orlando, FL) was adjusted to 75 mJ/pulse and its pulse frequency set to 5 Hz. The flow rate was set to 12.19 µL/min to ensure a 10% exclusion volume to avoid repeat hydroxyl radical exposure

28

. Each replicate was collected in a microcentrifuge tube containing a

quench mixture 10 µL of 140 mM H-Met-NH2 and 10 µL of 2 µM catalase to eliminate

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secondary oxidants such as remaining hydrogen peroxide, protein peroxides, superoxide, etc. The samples were incubated in the quench solution for 30 min at room temperature with pipette mixing. Control samples were handled in the same manner as those submitted to FPOP, but they were not laser irradiated; instead, they were incubated for 2 min with H2O2, after which the solution was added to the collection solution containing catalase and H-Met-NH2. All experiments were performed in triplicate for statistical analysis. Following irradiation, 2 µL of the sample (40 µL) was analyzed to determine the oxidation of adenine after HR-HRPF by UV at 260 nm (Thermo Scientific NanoDrop 2000c UV-Vis spectrophotometer) to ensure comparable amounts of available hydroxyl radical for oxidizing the analyte, as previously reported

33

. The adenine oxidation

amounts are similar among all tested samples with an average of around 53% oxidized adenine and CV lower than 5% (data not shown), which indicates the same level of hydroxyl radical available among all samples. The lyophilized powder was resuspended in 50 µL of 100 mM ammonium bicarbonate buffer. DTT (10 µL, 50 mM) was added and incubated at 65°C for 30 min to denature and reduce the protein. After cooling to room temperature, a 1:20 weight ratio of GluC:protein was added to the protein sample and incubated at room temperature for 2 hrs. Glu-C was deactivated by heating the sample to 95oC for 10 min, then after cooling the samples to room temperature a 1:20 weight ratio of trypsin was added to the samples for overnight digestion at 37 oC while rotating. Digestion was terminated by adding 12 µL DTT (50 mM) and heating the samples to 95oC for 10 min. Finally, when the samples were cooled to room temperature, 150 units of PNGase F was added into the digested

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Biochemistry

samples and incubated at 37 oC for 16 hrs. The reaction was terminated by adding 12 µL DTT (50 mM) and heating the sample to 95oC for 10 min. Samples were stored at -20°C until nanoLC-MS/MS analysis. HR-HRPF samples were analyzed on a LTQ Orbitrap XL (Thermo Fisher Scientific) controlled by Xcalibur 2.0.7 software (Thermo Fisher, San Jose, CA). Samples were loaded on a 10 cm column with a PicoFrit tip, bomb-packed with C18 reverse phase material (Magic, 0.075 mm × 100 mm, 5 µm, 300 Å, Michrom, Auburn, CA). Chromatography was performed using mobile phase A (0.1% formic acid in water) and B (0.1% formic acid in 80% acetonitrile) with a 160-min gradient consisting of 0-60% solvent B over 60 min at 135 µL/min, ramped to 100% B over 20 min, hold for 9 min, then returned to 0% B over 1 min and hold for 70 min. Peptides were eluted directly into the nanospray source of an LTQ Orbitrap XL using the bomb-packed column as the nanospray emitter. All data were acquired in positive ion mode. CID (collision induced dissociation) and/or ETD (electron transfer dissociation) were used to fragment peptides. The spray voltage was set to 2.0 kV and the temperature of the heated capillary was set to 200oC. In CID mode, full MS scans were acquired from m/z 400 to 1500 followed by eight subsequent MS2 scans on the top eight most abundant peptide ions. In ETD mode, the parent ions of all identified peptides under CID mode were listed in the parent ion mass list. ETD-based precursor activation was carried out for 100 ms, including chargestate-dependent with supplemental activation enabled. Precursor ions were isolated with width of 3 m/z units. HR-HRPF Data Analysis. Unoxidized gp120 controls, oxidized gp120 and oxidized gp120-b12 complex peptide sequences were initially identified using ByOnic V1.2-250

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software (Protein Metrics, CA, USA). The enzyme was defined to cleave the protein after lysine, arginine, asparatic acid and glutamine. Deamination on both asparagine and glutamine and all possible major oxidation modifications

25

were included as variable

modification for database searches. All tandem mass spectra assignments and sites of oxidation were verified manually due to peptide modification complexity. The LC-MS peak intensity of the digested peptides and corresponding oxidation products were used to calculate the average oxidation events per peptide in the sample. Peptide level quantitation is calculated by summing the ion intensities of all the oxidized peptide multiplied by the number of oxidation events required for the mass shift (e.g., one event for +16, two events for +32), then divided by the sum of the ion intensities of all unoxidized and oxidized peptide masses (eq 1):

=

(+16)  ∗ 1 + (+32)  ∗ 2 + (+48)  ∗ 3 + ⋯ (1)   + (+16)  + (+32)  + (+48)  + ⋯

where P denotes the oxidation events at peptide level and I donates the peak intensities of oxidized and unoxidized peptides. Gp120 control samples unilluminated by the UV laser, but otherwise identical to the experimental samples, were analyzed to ensure that background oxidation would not be intense enough to interfere with HR-HRPF data. Illumination-free controls showed less than 10% of the oxidation extent of gp120 sample with UV laser for peptides 19-31, 3763, 64-69, 178-197, 261-274, 386-396 and 397-404 (data not shown). The small amount of oxidation happening occurred at M or C due to sample preparation process (especially for residue M), protein expression and purification. The interference of this background

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Biochemistry

is not significant enough to affect the comparison of the oxidation extent between gp120 and gp120-b12 samples, and was subtracted from experimental samples. In cases where oxidation at specific sites can be identified either based on the mass differences between non-isomeric oxidation products or the presence of only a single oxidation site within a peptide, residue level quantitation is calculated from the LC-MS signal intensities of each peptide containing a specific oxidized amino acid (Ioxidized), relative to the total of all intensities associated with that peptide sequence (Ioxidized + Iunoxidized) using the following straightforward relationship24:

oxidation/residue $ =

∑   (2) ∑   + ∑  

In cases where the peptide isomers with oxidation at multiple adjacent sites in a single sequence that results in an identical mass shift, residue level quantitation is calculated from the fragment ion intensities from ETD to determine oxidation extent at specific residue site based on our previous studies

30, 31

. Briefly, oxidized peptide with multiple

sites of oxidation can generate both oxidized and unoxidized sequence ions in its tandem mass spectrum. The fractional oxidation of a given sequence ion is defined as the ratio between the oxidized sequence ion intensity to the sum of the intensity of the corresponding oxidized and unoxidized sequence ion (eq 3).

&(' ) =

(' )  (3) (' )  + (' ) 

Where &(' ) denotes the fractional oxidization of sequence ion $ (e.g., oxidized C3 ions generated by ETD), and (' ) denotes the intensity of the sequence ion i, whether the

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oxidized and unoxidized form. The absolute level of oxidation for a given amino acid residue $ is based on both the average oxidation event of peptide and the fractional oxidation of the corresponding sequence ions, as shown in eq 4:

()$*+,$(-⁄./0$*1/ $ = 3&(' ) − &(' 56 )7 (4)

Where P is the average oxidation events per peptide as derived from eq 1, and the term in square brackets is the fractional oxidation difference of two adjacent sequence ions $ and $-1. Multiplying the average number of oxidations per peptide by the fraction of that oxidation that occurs on a given amino acid residue yields the average oxidation events per residue. In cases where ETD did not yield sufficient product ions for quantification, CID was used to provide semi-quantitative information on changes in footprint at a residue level .

The protection by b12 binding is defined as the ratio of the difference of oxidation extent between gp120 sample alone and gp120-b12 binding sample to the oxidation extent of gp120 sample alone, as shown in eq 5: %Protection =

()$*+,$(-⁄./0$*1/ $; − ()$*+,$(-⁄./0$*1/ $;5?6= ()$*+,$(-⁄./0$*1/ $;

× 100 (5)

Glycosylation Analysis. An aliquot of each sample was denatured by incubating with 10 mM of dithiothreitol at 56 °C for an hour and alkylated by 55 mM of iodoacetamide for 45 minutes in dark prior to digestion with proteases optimized based on amino acid sequence of each target protein. Following digestion, the samples were deglycosylated by PNGaseF in the presence of O18-water which covert Asn residues carrying N-linked glycans to O18-Asp residues. The resulting peptides were separated on a 75 µm (I.D.) x 12 ACS Paragon Plus Environment

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15 cm C18 capillary column (packed in house, YMC GEL ODS-AQ120ǺS-5, Waters) and eluted into the nano-electrospray ion source of an Orbitrap Fusion™ Tribrid™ mass spectrometer (Thermo Fisher Scientific) with a 180-min linear gradient consisting of 0.5100% solvent B over 150 min at a flow rate of 200 nL/min. The spray voltage was set to 2.2 kV and the temperature of the heated capillary was set to 280 °C. Full MS scans were acquired from m/z 300 to 2000 at 120k resolution, and MS2 scans following collisioninduced fragmentation were collected in the ion trap for the most intense ions in the TopSpeed mode within a 3-sec cycle using Fusion instrument software (v1.1, Thermo Fisher Scientific). The resulting spectra were analyzed using SEQUEST (Proteome Discoverer 1.4, Thermo Fisher Scientific) with full MS peptide tolerance of 20 ppm and MS2 peptide fragment tolerance of 0.5 Da, and filtered using ProteoIQ (v2.7, Premier Biosoft) at the protein level to generate a 1% false discovery rate for protein assignments. Site occupancy was calculated using spectral counts of O18-Asp peptides to the identical peptide containing Asn. The sole exception is N88, which was wholly unobserved as glycosylated or non-glycosylated by this method. Glycosylation of this site was assigned based on the high abundance of the N→D substitution of this site after PNGase F treatment during HR-HRPF data acquisition and analysis, as detailed above. A site was assigned as occupied for the purposes of modeling if the site was predominantly glycosylated across all peptides in which the site was observed. Notably, sites N241 and N262, which are co-resident on a single peptide and were detected as individually glycosylated 56% of the time, but simultaneous glycosylation was detected for only 6% of observed peptides. Thus, the predominant state was for only one site to be

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glycosylated. Given their equal rate of occupancy, we arbitrarily chose the N241 for glycosylation in the model. Model Building. Blastp 34 was used to find 3D structures from the protein data bank (PDB) with sequences similar to JR-FL. A modified JR-FL has been crystallized previously (PDB 2B4C), however, the N- and C-termini were truncated and the V1/V2 region was substituted with a GAG tripeptide. The model used here is based on 2B4C and employs additional crystal structures (PDBID 4NCO, 3JWD, 4HPO, 4R4H, 4TVP, and 9B4C) to model a complete JR-FL. Modeller 35 incorporated each of these structures (see alignments in Supplementary Material, Figure S1) to generate five models of the complete gp120. Model Glycosylation. Previous work revealed that expression of gp120 from Ba-L (a Clade B isolate) in 293F cells resulted in a mixture of complex and high mannose glycans, and that interaction of the B-clade gp120 with the b12 bNAb was increased by expression in cells that increased high mannose glycosylation 36. Since the full diversity of possible glycan structures are impractical to simulate even for individual compositions of complex glycans, Man9GlcNAc2 structures were modeled into all sites assigned as glycosylated, as this glycan is a single, biologically relevant structure that explores the extended reach of glycans in examining both glycan-antibody interactions and glycan shielding of the antibody binding surface. GLYCAM-Web (www.glycam.org) was used to generate 3D structures of the common rotamers of Man9GlcNAc2. These structures were superimposed onto the Asn side chain at each assigned glycosylation site. The glycan structure was adapted to the local protein environment and any previous glycans in the following manner: the four most populated conformations of N-linked Asn side chains 14 ACS Paragon Plus Environment

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found in the PDB were assessed in series by altering the chi, phi and psi angles of the Nlinked glycan. Any vdW overlaps between the glycan and the glycoprotein were minimized by altering the torsion angles of the Asn-GlcNAc and interglycosidic linkages by ±20° in 5° increments. The first glycan rotamer and Asn side chain conformer which allowed the glycan to fit without large vdW overlaps was added to the glycoprotein structure. Model Selection. A single model of JR-FL was chosen based on the number of experimentally determined glycosylation sites that were accessible for glycosylation and the DOPE score provided by Modeller. A recent crystal structure of a complete JR-FL (PDBID 5FYK)

37

, released during manuscript preparation, allowed validation of the

model generated here. A Needleman-Wunsch alignment using a BLOSUM-62 matrix

38

gave a relatively large RMSD of 5.2 Å over 450 atom pairs. However, most of the deviation is in the flexible N and C-terminal domains that are ordered by the presence of the gp41 in the crystal structure. When these regions are discounted the RMSD drops to 1.0 Å over 303 atom pairs indicating that the homology model successfully predicted the remaining 3D structure. Molecular Dynamics Simulation. All simulations were performed with the CUDA implementation of PMEMD

39, 40

in the Amber14 software suite

41

. The carbohydrate

was modeled using the GLYCAM06j-1 force field 42, while the Amber ff14SB force field 43

was employed for the protein. A Berendsen barostat with a time constant of 1 ps was

employed for pressure regulation, while a Langevin thermostat with a collision frequency of 2 ps-1 was employed for temperature regulation. A nonbonded interaction cutoff of 8 Å was employed. Long-range electrostatics were treated with the particle-mesh Ewald 15 ACS Paragon Plus Environment

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(PME) method

44

.

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Covalent bonds involving hydrogen were constrained with the

SHAKE algorithm allowing a time step of 2 fs 45. Each system was placed in a cubic box of TIP5P water

46

with an 8 Å buffer between

solute and the periodic boundary. Five sodium ions were added to neutralize the overall charge. Energy minimization was performed for 20,000 steps (10,000 steepest decent, followed by 10,000 conjugant gradient) with Cartesian restraints (5 kcal/mol-Å2) on solute heavy atoms. This was followed by a 400 ps solvent equilibration phase at 300 K under nPT conditions. Cartesian restraints (5 kcal/mol-Å2) were employed on the Cα atoms of the first and last three residues (N- and C-terminal) during a 1 ns structural equilibration phase and 500 ns production phase (nPT). Structure of gp120 (JR-FL) in Complex with the b12 Antibody. A structure-based sequence alignment of gp120 (HXBc2) in complex with the b12 Ab (PDB 2NY7) to 100 snapshots from the MD simulation of JR-FL was performed using Sequoia

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, with an

RMSD of 4.14 Å for 245 equivalent matches based on aligning to the original model, with a Sequoia alignment score of 0.08 Based on each alignment, an estimation of the contact area between b12 and JR-FL was obtained by averaging the percentage change in the respective solvent accessibilities

48

for each snapshot in the presence and absence of

the b12 structure.

RESULTS Glycosylation analysis of gp120 and the gp120-b12 complex.

The recombinant

glycoprotein gp120 used in this study was expressed as a 477-amino acid mature protein containing intact N and C termini, five variable regions (V1/V2, V3, V4, and V5) and five conserved regions (C1 to C5). Following Glu-C and trypsin digests of gp120, thirty-

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two unique peptides were identified by CID based MS/MS data, covering 72% of the overall sequence. Eighteen highly occupied N-linked glycosylation sites were identified. The details of the oxidation sites and glycosylation sites are listed in Figure 1. HR-HRPF of gp120-b12 Complex. Twenty-nine peptides were seen to be modified by FPOP footprinting. Sixty-one oxidized residues were identified in these modified peptides based on Byonic database search and verified manually from the CID and/or ETD MS/MS spectra. Almost one in five residues in the peptides detected was modified, indicating a high yield of protein labeling though the short FPOP reaction window. The oxidation extent of the modified twenty-nine peptides was determined at the peptide level by measuring the intensity of the oxidized peptide ion compared to the sum of oxidized and unoxidized peptide ions as calculated by Eq 1. The side-by-side comparison of the oxidation extent of sequenced peptides in gp120 and gp120-b12 samples was shown in Figure 2. The overall number of average oxidations per peptide for different peptides varies significantly from 0.003 for peptide 179-192 to 3 for peptide 198-207. Most peptides show comparable amounts of oxidation between binding and non-binding samples. Eleven peptides including 92-97, 98-106, 156-168, 173-178, 179-192, 316-327, 336-343, 358-370, 410-419, 433-440, and 467-476 showed a significant decrease in oxidation extent upon b12 binding, indicating a structural event that shielded the sites from the radical. To investigate structural changes upon binding between gp120 and b12 at a high spatial resolution, the CID- and ETD-based tandem mass spectrum of these eleven peptides were analyzed. A representative ETD spectrum is shown in Figure 3. Based on the CID/ETD

JRFL

---------- ---------- ---------- VEKLWVTVYY GVPVWKEATT

50

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TLFCASDAKA YDTEVHNVWA THACVPTDPN PQEVVLENVT EHFNMWKNNM V1V2-> VEQMQEDIIS LWDQSLKPCV KLTPLCVTLN CKDVNATNTT NDSEGTMERG PVVSTQLLLN GSLAEEEVVI RSDNFTNNAK TIIVQLKESV EINCTRPNNN ENKTIVFNHS SGGDPEIVMH SFNCGGEFFY CNSTQLFNST WNNNTEGSNN 80% protection from HR-HRPF upon b12 binding are colored red; residues that showed between 40% and 80% protection from HR-HRPF upon b12 binding are colored orange; residues that showed statistically significant protection from HRHRPF 80% protection from HR-HRPF upon b12 binding are colored red; residues that showed between 40% and 80% protection from HR-HRPF upon b12 binding are colored orange; residues that showed statistically significant protection from HR-HRPF 80% protection from HR-HRPF upon b12 binding are colored red; residues that showed between 40% and 80% protection from HR-HRPF upon b12 binding are colored orange; residues that showed statistically significant protection from HR-HRPF 80% protection from HR-HRPF upon b12 binding are colored red; residues that showed between 40% and 80% protection from HR-HRPF upon b12 binding are colored orange. Residues showing no protection from HR-HRPF upon b12 binding are colored blue. Glycans are labeled and shown as 3DSNFG symbols, which are positioned at each residue’s ring center.

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α2 helix, as well as T450 in the center of the β22/β23 strand both showed a greatly decreased affinity for the b12 antibody 50, supporting a potential impact of b12 binding on this region. It is also possible that the N339 glycan is involved in interactions that are not properly captured by our model. Interestingly, the CATNAP database56 shows a strong positive correlation between an intact N339 glycosylation sequon and b12 binding, suggesting that glycosylation of N339 is involved, directly or indirectly, in the energetics of b12 binding. Previous deglycosylation studies by Koch and co-workers did not examine elimination of the N339 or the N362 glycosylation sites 18. While it is not apparent how the N339 glycan could interact directly with the b12 antibody, perhaps the N339 glycan contributes indirectly via another glycan, which interacts directly with b12. Unprotected Domains. The C2 and V4 domains both had at least one residue probed by HR-HRPF, but no residues that showed protection upon b12 binding. For the C2 domain, eight residues were probed by HR-HRPF (C205, C218, C239, S274, D275, F277, L288, and K289), including three cysteines that are highly sensitive to changes in solvent accessibility. This domain is distal to the b12 antibody binding interface, and is largely made up of flexible loops and small anti-parallel β-sheets. All of the HR-HRPF probed residues are found on regions lacking secondary structure in both the MD model and the X-ray crystal structure of the stabilized gp120-b12 complex, indicating that the binding of b12 does not appreciably change the structure and dynamics of the loops. The residues in the β-sheets were silent in this HR-HRPF experiment. Interestingly, several residues in the C2 domain were found by mutagenesis to affect b12 binding affinity, mostly among residues that are conserved among most gp120 sequences 50. Unfortunately, in no

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case did we receive HR-HRPF data directly on a residue mutated by Pantophlet and coworkers, and the pattern of mutations that changed binding affinity is by no means clear; several of the mutations that decreased b12 binding affinity occurred on the 223-239 stretch of the C2 domain that was unoxidized by HR-HRPF (Figure 2). The V4 domain had one residue at its extreme C-terminus probed by HR-HRPF: I414, which can be seen near the N-terminal end of the β19 strand in Figure 7a.

The

interaction with b12 occurs at the C-terminal end of the long β19 strand, and the lack of HR-HRPF protection of I414 upon b12 binding suggests that the N-terminal end of this strand remains stable in the absence of b12 binding. No mutagenesis data for this residue was reported, and CATNAP database analysis showed no correlation between this amino acid identity and b12 affinity.

Conclusion We demonstrate the ability of the hydroxyl radical-mediated protein footprinting combined with molecular modeling to examine the protein–protein interactions between a highly glycosylated mature gp120 and its bNAb b12. The data presented here identified the highly occupied glycosylation sites and probed the solvent accessibility of sixty-one residues in full-length, glycosylated JR-FL gp120, and identify 23 residues protected upon b12 binding. Analysis of our results in the context of a model of full-length, glycosylated gp120 indicate a prominent role of V1/V2 interactions with the light chain of the b12 antibody, as well as potential roles for the N362 glycan in interacting directly with b12. HR-HRPF was able to detect both direct interactions and conformational changes upon b12 binding without the need to introduce mutations or site-specific chemical labels that could alter the native conformation we seek to probe.

The 37

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combination of HR-HRPF for providing biophysical data on the full-length glycosylated gp120 protein, combined with molecular modeling to provide a context to interpret changes in HR-HRPF protection, is a powerful combination. Further studies examining the interactions of gp120 with other bNAbs are underway.

Associated Content Supporting Information Supporting Information available includes a tabulation of glycosylation sequon occupancy for gp120 samples used for this study, and a figure showing the gp120 sequence alignments used to by Modeller to generate the homology model.

Funding Information This research is supported by the National Institute of General Medical Sciences (1R01GM096049-01A1 and R01GM100058). Instrumentation used for analysis were funded in part by the National Institute of General Medical Sciences-funded "Research Resource for Integrated Glycotechnology" (P41 GM103390) from the National Institutes of Health. Glycosylation occupancy identification by L.W. was supported in part by the National Center for Biomedical Glycomics (P41 GM103490). S.L. acknowledges funding from a Gates Foundation grant (OPP1033112) and the National Institute of Allergy and Infectious Diseases (AI082274 and AI082676). R.J.W was supported by the National Institutes of Health (U01 CA207824 and P41 GM103390).

Acknowledgements

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Figures depicting 3D structures were created using Visual Molecular Dynamics (VMD) 57

. J.S.S. discloses a significant ownership share of Photochem Technologies, LLC, a

small company that is active in the area of hydroxyl radical protein footprinting.

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TOC Graphic b12 Light Chain

V1/V2

V3

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