LETTER pubs.acs.org/JPCL
Structural Dynamics of EcDOS Heme Domain Revealed by Time-Resolved Ultraviolet Resonance Raman Spectroscopy Samir F. El-Mashtoly,*,† Minoru Kubo,‡ Satoru Nakashima,‡ Toru Shimizu,§ and Teizo Kitagawa*,‡ †
Lehrstuhl f€ur Biophysik, Ruhr-Universit€at Bochum, Bochum, Germany Picobiology Institute, Graduate School of Life Science, University of Hyogo, Hyogo, Japan § Institute of Multidisciplinary Research for Advanced Material, Tohoku University, Sendai, Japan ‡
bS Supporting Information ABSTRACT: Protein dynamics on the subnanosecond to microsecond time scale was investigated for the isolated heme domain of a gas sensor protein, EcDOS, with time-resolved ultraviolet resonance Raman (UVRR) spectroscopy. Rapid structural changes (