Structural Dynamics of EcDOS Heme Domain Revealed by Time

Chem. Lett. , 2011, 2 (17), pp 2212–2217. DOI: 10.1021/jz201017y. Publication Date (Web): August 17, 2011. Copyright © 2011 American Chemical Socie...
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Structural Dynamics of EcDOS Heme Domain Revealed by Time-Resolved Ultraviolet Resonance Raman Spectroscopy Samir F. El-Mashtoly,*,† Minoru Kubo,‡ Satoru Nakashima,‡ Toru Shimizu,§ and Teizo Kitagawa*,‡ †

Lehrstuhl f€ur Biophysik, Ruhr-Universit€at Bochum, Bochum, Germany Picobiology Institute, Graduate School of Life Science, University of Hyogo, Hyogo, Japan § Institute of Multidisciplinary Research for Advanced Material, Tohoku University, Sendai, Japan ‡

bS Supporting Information ABSTRACT: Protein dynamics on the subnanosecond to microsecond time scale was investigated for the isolated heme domain of a gas sensor protein, EcDOS, with time-resolved ultraviolet resonance Raman (UVRR) spectroscopy. Rapid structural changes (