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Structure of the Escherichia coli K2 Capsular Antigen. Stereochemical Configuration of the Glycerophosphate and Distribution of Galactopyranosyl and Galactofuranosyl Residues? Werner Fischer,* M. Alexander Schmidt, Barbara Jann, and Klaus Jann
ABSTRACT:
The Escherichia coli K2 capsular antigen is known to be composed of a-D-galactopyranosyl( l-2)glycerophosphate and a-D-galactofuranosyl( 1-2)glycerophosphate units which are connected by phosphodiester bonds to C-4 of the galactopyranosyl and C-5 or C-6 of the galactofuranosyl moieties. In the present study the glycerophosphates were released by two different procedures and shown to have the sn-glycero3-phosphate stereochemical configuration. In the first, the chain was fragmented by Smith degradation to glycerophosphothreitol from which the glycerophosphatewas released by alkali hydrolysis. The structure-dependent low recovery of a-glycerophosphate (
