Synthesis and biological evaluation of bolaamphiphilic sophorolipids

May 21, 2018 - New synthetic pathways are proposed for the synthesis of a new set of bolaamphiphilic derivatives starting from microbiologically produ...
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Synthesis and biological evaluation of bolaamphiphilic sophorolipids Elisabeth I. P. Delbeke, Jonas Everaert, Olivier Lozach, Tony Le Gall, Mathieu Berchel, Tristan Montier, Paul-Alain Jaffrès, Petra Rigole, Tom Coenye, Martha Brennich, Niki Baccile, Sophie Roelants, Wim Soetaert, Inge N. A. Van Bogaert, Kevin Marcel Van Geem, and Christian Victor Stevens ACS Sustainable Chem. Eng., Just Accepted Manuscript • DOI: 10.1021/ acssuschemeng.8b01354 • Publication Date (Web): 21 May 2018 Downloaded from http://pubs.acs.org on May 21, 2018

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Synthesis and biological evaluation of bolaamphiphilic sophorolipids Elisabeth I. P. Delbekea,b, Jonas Everaerta,c, Olivier Lozachd, Tony Le Galle,f, Mathieu Bercheld,e, Tristan Montiere,f,g,h, Paul-Alain Jaffrèsd,e, Petra Rigolei, Tom Coenyei, Martha Brennichj,k, Niki Baccilel, Sophie L. K. W. Roelantsc,m, Wim Soetaertc,m, Inge N. A. Van Bogaertn, Kevin M. Van Geemb, Christian V. Stevensa* a

SynBioC, Department of Green Chemistry and Technology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium. E-mail: [email protected] b LCT, Department of Materials, Textiles and Chemical Engineering, Ghent University, Technologiepark 914, 9052 Ghent, Belgium. c InBio, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium. d Université de Brest, CEMCA, CNRS UMR 6521, IBSAM, 6 avenue Le Gorgeu, 29238 Brest, France. e IBiSA SynNanoVect platform, IBSAM, Université de Brest, Faculté de médecine Morvan, avenue Camille Desmoulins, 46 rue Félix Le Dantec, CS 51819, 29218 Brest Cedex 2, France. f Université de Brest, INSERM UMR 1078, IBSAM, Faculté de médecine Morvan, avenue Camille Desmoulins, 46 rue Félix Le Dantec, CS 51819, 29218 Brest Cedex 2, France. g CHRU de Brest, Service de Génétique Moléculaire et d’Histocompatibilité, 5 avenue de Maréchal Foch, 29609 Brest cedex, France. h DUMG, Faculté de Médecine et des Sciences de la Santé, 22 avenue Camille Desmoulins, 29328 Brest, France. i Laboratory of Pharmaceutical Microbiology, Ghent University, Ottergemsesteenweg 460, 9000 Ghent, Belgium. j European Molecular Biology Laboratory, Synchrotron Crystallography Group, 71 Avenue des Martyrs, 38042 Grenoble, France. k Institut Laue-Langevin, Grenoble, Beamline D16, Cedex 9, 38042, Grenoble, France. l Sorbonne Université, UPMC Univ Paris 06, CNRS, Collège de France, Laboratoire de Chimie de la Matière Condensée de Paris, UMR 7574, F-75005 Paris, France. m Bio Base Europe Pilot Plant (BBEU), Rodenhuizenkaai 1, 9042 Ghent (Desteldonk), Belgium. n Centre for Synthetic Biology, Department of Biotechnology, Ghent University, Coupure Links 653, 9000 Ghent, Belgium.

Abstract New synthetic pathways are proposed for the synthesis of a new set of bolaamphiphilic derivatives starting from microbiologically produced sophorolipids. A total set of 43 new derivatives was synthesized via reductive amination of a previously synthesized sophorolipid aldehyde with diamines and primary amines. The new derivatives were evaluated for their antimicrobial activity against Gramnegative and Gram-positive bacteria. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) values were determined for the active compounds. Transfection efficiencies were also evaluated for some of the deprotected derivatives via the assessment of their capacity to transfect three different eukaryotic cell lines in vitro. Finally, the self-assembly properties were evaluated for the deprotected derivatives. Antimicrobial activities were mostly observed for the peracetylated mono- or dicationic bolaamphiphiles and only the deprotected monocationic bolaamphiphile with an octadecyl chain on the nitrogen atom was eligible for the evaluation of its transfection properties. Evaluation of the self-assembly properties indicated that the presence of an octadecyl chain was necessary for micelle formation. Both micelle formation and the net charge of the compounds seem to have an influence on the antimicrobial activity and transfection efficiency. These results are promising for use of bolaamphiphilic sophorolipids in medical and self-assembly applications. Keywords Sophorolipids, chemical modification, bolaamphiphiles, antimicrobial activity, transfection efficiency, quaternary ammonium salt, self-assembly 1 ACS Paragon Plus Environment

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Synopsis Sophorolipids, renewable biosurfactants, are used for the synthesis of innovative bolaamphiphilic compounds for application in the medical sector. Introduction Bolaamphiphiles possess not one but two hydrophilic heads, coupled by a hydrophobic linker. Currently, there is a lot of interest in synthetic bolaamphiphiles because of the unusual architectures created by such molecules.1 They form monolayer membranes which can organize into micelles, vesicles, nanotubuli, etc. These supramolecular features often arise spontaneously, they are so-called self-organizing structures. The best known natural examples of bolaamphiphiles are the tetraether lipid membranes of Archaea which are able to grow under very extreme temperatures and at very high salt concentrations. In this context bolaamphiphiles likely contribute to the stabilization of membranes.1-2 As a result, they can for example be used in drug or nucleic acid delivery applications to form or stabilize vesicles.3-4 After all, most liposomes currently used for this purpose face stability problems such as leakage of the content before reaching the target location.5 Moreover, bolaamphiphiles can be used as membrane-spanning linkers in biosensors to detect proteins, antibodies, viruses, etc.6 They can also serve as channels for ion transport.1 Fluorescent bolaamphiphiles can be used as transmembrane probes for lipid imaging to visualize for example ether lipids in the brain.7 In view of the growing ecological awareness and the necessity to develop alternatives for fossil resources, the production of amphiphiles from renewable resources is becoming increasingly important. However, among the synthetic bolaamphiphiles which are described in literature, only a few are produced from renewable resources.8 These renewable based bolaamphiphiles are mostly fatty acid - for example derived from vernonia and castor oil - and sugar based derivatives.9-19 The use of glutamic acid, proline and vitamin C was also reported for the synthesis of bolaamphiphilic compounds.20-22 Examples of naturally occurring bolaamphiphiles are the carotenoid glycoside crocin 1, present in saffron up to 25%, and sophorolipid acid 2 (Figure 1).23-25 Sophorolipids are a class of glycolipid biosurfactants which are produced by different yeast species, mainly Starmerella bombicola, from renewable resources. They consist of a hydrophilic sophorose head and a hydrophobic fatty acid tail. The main sophorolipid fermentation products are C18:1 sophorolipid acid 2 and diacetylated C18:1 sophorolipid lactone 3 which both include oleic acid in their structure (Figure 1). Natural sophorolipids feature interesting biological activities such as anti-cancer, antimicrobial, dermatological, immunoregulatory, spermicidal and antiviral activity.26-27 Moreover, they possess self-assembly properties with a high variety in the type of nanostructures formed from different sophorolipid derivatives.28-32 For example, C18:1 sophorolipid acid 2 forms micelles with the charge dependent on the pH, while its saturated derivative forms nanoscale ribbons with supramolecular chirality.31 Recently, the fermentative production of sophorolipid based bolaamphiphiles with two sugar heads was also reported.33-34 In this work, the synthesis of a new class of bolaamphiphilic sophorolipids is described starting from the major microbial fermentation product, i.e. diacetylated sophorolipid lactone 3. A modification pathway previously developed in our group is used for the synthesis of an intermediate sophorolipid aldehyde 6 (Scheme 1).35 This aldehyde intermediate is then further modified towards different nitrogen containing bolaamphiphilic sophorolipids. With this procedure, the length of the linker can be customized and optimized, which is not the case for derivatives produced via fermentation. The new bolaamphiphilic derivatives were evaluated regarding their antimicrobial, transfection and selfassembly properties. Previously synthesized quaternary ammonium sophorolipids proved to have good antimicrobial and transfection properties when an octadecyl chain was present on the nitrogen atom.35-36 With the 2 ACS Paragon Plus Environment

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described reaction procedures, an overall yield of 23% was obtained for the quaternary ammonium sophorolipids with the ozonolysis and reductive amination being the crucial steps.37 If the yields of these reaction steps could be heightened towards 90% in an industrial setting by avoiding automated chromatography purification, the overall yield of the reaction pathway could be raised to 71%. The carbon efficiency and atom economy were respectively 69% and 62% for the sophorolipid aldehyde 3 and respectively 70% and 48% for the quaternary ammonium sophorolipids. The valorization of the methyl 9-oxononanoate byproduct resulting from the ozonolysis step is taken into account. For all reaction steps, the use of green solvents was aimed at, resulting in the replacement of dichloromethane with methanol for the ozonolysis step.38

Figure 1. Crocin 1, sophorolipid acid 2 and sophorolipid lactone 3

Scheme 1. Modification pathway towards sophorolipid aldehyde 6

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Experimental section Determination of the antimicrobial activity. Antimicrobial activity of all bolaamphiphilic sophorolipids against E. coli LMG 8063, K. pneumoniae LMG 2095, P. aeruginosa PAO1, S. aureus ATCC 6538 and S. aureus Mu50 was assessed by a broth microdilution method (CLSI, 2012).39 Strains with LMG designation were obtained from the BCCM/LMG Bacteria Collection (Ghent, Belgium), while strain ATCC 6538 was obtained from the American Type Culture Collection (Manassas, VA). S. aureus strain Mu50 was a kind gift of P. Vandamme (Ghent, Belgium). All strains were grown aerobically at 37 °C on Mueller Hinton agar (LabM, Heywood, UK). The minimal inhibitory concentration (MIC) that inhibited growth completely compared to the untreated control and the minimal bactericidal concentration (MBC) at which no surviving organisms can be recovered were used as a measure of activity. MIC and MBC values were determined using flat-bottomed 96-well microtiter plates (TPP, Trasadingen, Switzerland). Concentrations of compounds tested ranged from 1.22 to 2500 µg/mL in Mueller Hinton Broth (LabM). The inoculum was standardized at approximately 5 x 104 colony forming units/mL. The plates were incubated at 37 °C for 24 h, and the optical density was determined at 590 nm using a multilabel microtiter plate reader (Envision Xcite, PerkinElmer LAS, Waltham, MA). Liposome formulation. The liposomal solutions were prepared by the lipid film hydration method. A 1.5 mM solution (in 1 mL) of each compound was prepared in chloroform, formulated with or without DOPE (1:1 compound/DOPE) and evaporated under reduced pressure to produce a thin lipid film. Water (1 mL) was added to rehydrate this lipid film in a time period of 7 days at room temperature. The solution was vortexed (10 s) and sonicated (30 min at 50 °C) at 45 kHz using a VWR ultrasonic bath. The size and zeta potential were determined for each liposomal formulation. DNA complexation. Lipoplexes were prepared by mixing pDNA (pEGFP-Luc, Clontech) with each liposomal solution in OptiMEM (Gibco). Addition of pDNA to the liposomal solutions was performed at concentrations corresponding to CR ranging from 1 to 4. The obtained mixtures were incubated at room temperature for 1 h before being subjected to electrophoresis in a 0.8% agarose gel at 100 V, 90 mA. The gel was stained with SYBRgold nucleic acid gel staining (Life Technologies) and visualized under UV light using a UV trans-illuminator (Fischer Bioblock). Transfection efficiency. The in vitro reporter gene assay via luciferase measurement was carried out as reported previously.40-41 Data were expressed as relative light units (RLU) per milligram of total proteins (means ± SD with n=3). Lipofectamine 3000 (Invitrogene) was used as standard. Cell viability. The Vialight kit (Lonza) was used to estimate the viability of the cells following transfection. For this purpose, 48 h after exposition to the lipoplexes to evaluate, cells were lysed and their ATP content was determined, as recommended by the manufacturer. Non-transfected cells were used to express viability results in the form of percentages (% of the reference). Small Angle X-ray Scattering (SAXS). SAXS experiments are performed at 25 °C immediately after sample preparation on the BioSAXS BM29 beamline at the ESRF synchrotron facility (Grenoble, France) using 12.5 keV energy and a sample-to-detector distance of 2.867 m, imposed by the beamline standard configuration. The energy is calibrated by measuring the LI and LIII edges of platinum and the sample-to-detector distance is determined using silver behenate (dref = 58.38 Å). (http://www.esrf.eu/home/UsersAndScience/Experiments/MX/About_our_beamlines/bm29.html).42 For this experiment, the automatic samples changer for liquids was employed using the 96-well plates 4 ACS Paragon Plus Environment

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and about 100 µL of each sample.43 The liquid sample is automatically loaded into a 1.8 mm quartz glass capillary and ten acquisitions of 1 s each are taken as the sample passes the beam. Individual frames are manually controlled for systematic changes and averaged for better statistics if none are found. Eventual changes can be either due to intrinsic sample heterogeneity or radiation damage. The signal of the Pilatus 1M 2D detector, used to record the data, is integrated azimuthally with PyFAI to obtain the I(q) vs. q spectrum ( =

  , 

, where 2θ is the scattering angle) after masking

systematically wrong pixels and the beam stop shadow.44 Absolute intensity units were determined by measuring the scattering signal of water (0.0163 cm-1). Radii of gyration, Rg, have been calculated by the Guinier analysis of the SAXS data using the SasView software, available free of charge at the developer’s website (http://www.sasview.org). Results and discussion Synthesis of the new derivatives A first set of bolaamphiphilic sophorolipid derivatives was synthesized via reductive amination of sophorolipid aldehyde 6 with several diamines. A set of secondary diamines 7 with an ethylene-, hexamethylene, or o-phenylenelinker and methyl, butyl or octadecyl groups was selected for the synthesis of the desired bolaamphiphilic sophorolipids (Figure 2). With this set of amines, it was envisioned to assess the influence of the length of the alkyl linker, the presence of an aromatic group in the linker and the length of the substituents on the properties of the derivatives. Since only N,N’-dimethylethylenediamine 7a and N,N’-dimethylhexamethylenediamine 7b are commercially available, other diamines had to be synthesized.

Figure 2. N,N'-dialkyldiamines 7 as substrates for the reductive amination towards bolaamphiphilic sophorolipids

The intermediate diamides 10 were synthesized via a Schotten-Bauman reaction with acid chlorides 8 and diamines 9 (Scheme 2). Subsequently, these intermediate diamides were reduced with a borane tetrahydrofuran complex towards the desired N,N’-dialkyldiamines 7. With this procedure, a total set of eight different N,N’-dialkyldiamines 7 was available, including the commercially available N,N’-dimethylethylenediamine 7a and N,N’-dimethylhexamethylenediamine 7b. These intermediate diamines were not fully characterized and therefore reaction yields were not determined. The synthesis of the desired bolaamphiphilic sophorolipids 11 was performed according to the reaction conditions described for the previously synthesized sophorolipid amines (Scheme 2).35 The reductive amination of sophorolipid aldehyde 6 with N,N’-dialkyldiamines 7a-d resulted in the synthesis of the desired bolaamphiphilic sophorolipids 11a-d. However, reductive amination with N,N’-dialkyldiamines 7e-h was not successful. In the case of N,N’-dialkyldiamines 7e, 7f and 7h, poor solubility of the diamines in the reaction solvent prevented the synthesis of the desired bolaamphiphilic sophorolipids. When tetrahydrofuran was evaluated as solvent instead of methanol, no reductive amination occurred either. In the case of N,N’-dialkyldiamine7g, NMR-analysis indicated 5 ACS Paragon Plus Environment

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that the reductive amination did occur partially, but no complete conversion could be obtained due to the steric hindrance caused by the close proximity of the two butyl groups.

Scheme 2. Synthesis of N,N'-dialkyldiamines 7 and reductive amination towards bolaamphiphilic sophorolipids 11

The formation of another class of bolaamphiphilic sophorolipids was evidenced in the course of the evaluation of the reductive amination of sophorolipid aldehyde 6 with primary amines. When the same reaction conditions were applied as for the reductive amination with secondary amines, a mixture of secondary sophorolipid amines and bolaamphiphilic sophorolipid amines was obtained. The selective formation of N-alkyl bolaamphiphilic sophorolipid amines 12 was accomplished by using only 0.5 equivalents of primary amine and applying the same reaction conditions as described for the N,N’dialkyl bolaamphiphilic sophorolipids 11 (Scheme 3).

Scheme 3. Reductive amination with primary amines towards bolaamphiphilic sophorolipids 12

The set of four N,N’-dialkyl bolaamphiphilic sophorolipids 11 was transformed into the deprotected N,N’-dialkyl bolaamphiphilic sophorolipids 13, peracetylated dicationic bolaamphiphilic sophorolipids 14 and deprotected dicationic bolaamphiphilic sophorolipids 15 (Scheme 4). The work of De Vos, De Coen et al. showed that the double quaternization of a bis(7-azabicyclo[2.2.1]heptane) derivative with an ethylene linker to a dicationic compound was unsuccessful, even upon reaction with 20 equivalents of methyl iodide at room temperature.45-46 Therefore, it could be anticipated that the large charge repulsion on the small ethylene linker could also inhibit the formation of the dicationic

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bolaamphiphilic sophorolipids 14a and 14c. However, NMR-analysis clearly confirmed the successful synthesis of both compounds.

Scheme 4. Modification of N,N'-dialkyl bolaamphiphilic sophorolipids 11 via quaternization and deprotection

Alternative procedures were evaluated for the synthesis of N,N’-dialkyl bolaamphiphilic sophorolipids with octadecyl groups on the nitrogen atom. At first, the introduction of the octadecyl groups was attempted via quaternization of the N,N’-dimethyl bolaamphiphilic sophorolipids 11a and 11b with octadecyl iodide. However, no quaternization occurred in acetonitrile or toluene as solvent after 72 h. An alternative procedure was designed which comprised the formation of secondary bolaamphiphilic sophorolipid amines as intermediates. To prevent similar overalkylations which resulted in the formation of the N-alkyl bolaamphiphilic sophorolipid amines 12, the mixture of sophorolipid aldehyde 6 and diamine was stirred for 1 h at room temperature prior to the addition of sodium cyanoborohydride and acetic acid. Attempts to alkylate these secondary bolaamphiphilic sophorolipid amines with octadecyl iodide were unsuccessful. Also reductive amination of these secondary bolaamphiphilic sophorolipid amines with octadecanal failed, although this dual reductive amination procedure proved to work for the synthesis of N,N’-dibutyl bolaamphiphilic sophorolipid 11c. The set of four N-alkyl bolaamphiphilic sophorolipids 12 was also transformed into the deprotected N-alkyl bolaamphiphilic sophorolipids 16, peracetylated monocationic bolaamphiphilic sophorolipids 17 and deprotected monocationic bolaamphiphilic sophorolipids 18 (Scheme 5). To extend the set of N,N’-dialkyl bolaamphiphilic sophorolipids 11, reductive amination of a C12 sophorolipid aldehyde 20 was performed with N,N’-alkyldiamines 7a, 7b and 7d (Scheme 6). This C12 sophorolipid aldehyde was obtained via incorporation of petroselinic acid into the sophorolipid structure, resulting in an aldehyde intermediate with a longer lipid chain after the ozonolysis reaction.47 The resulting N,N’-dialkyl bolaamphiphilic sophorolipids 11i, 11j and 11k were subsequently quaternized with methyl or butyl iodide towards the peracetylated dicationic bolaamphiphilic sophorolipids 14e-h. In view of the antimicrobial activities for the C9 sophorolipid aldehyde derived bolaamphiphilic compounds (vide infra), only the peracetylated dicationic bolaamphiphilic sophorolipids 14e-h were deprotected towards the dicationic bolaamphiphilic sophorolipids 15e-h.

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Scheme 5. Modification of N-alkyl bolaamphiphilic sophorolipids 12 via quaternization and deprotection

Scheme 6. Modification of C12 sophorolipid aldehyde 19 towards N,N'-dialkyl bolaamphiphilic sophorolipipds

Different sustainable chemistry metrics were evaluated for the synthesis of the bolaamphiphilic sophorolipid derivatives (Table 1). Overall reaction yields ranging from 8 to 33% were obtained for the new derivatives, with the ozonolysis and reductive amination being the crucial steps.37 If the yields of these reaction steps could be heightened towards 90% in an industrial setting by avoiding automated chromatography purification, the overall yield of the reaction pathways could be raised to 71%. For the calculation of the carbon efficiency (CE) and the atom economy (AE), the valorization of the methyl 9-oxononanoate byproduct resulting from the ozonolysis step is taken into account. The CE estimates the percentage of carbon from the reactants that is contained in the final product. This metric does not take into account the amount of solvent used and waste generated during the process. The AE estimates the total amount of reactants that are incorporated into the final product. Also here, only the reactants which are incorporated into the final product are taken into account. Solvent use and waste 8 ACS Paragon Plus Environment

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generation are not quantified. Metrics which do take the solvent use and waste generation into account are not qualified here since these largely depend on the scale at which the reactions are performed and scale-up of the reaction procedures was out of the scope of this work. For all reaction steps, the use of green solvents was aimed at. Table 1. Overview of the sustainable chemistry metrics for the production of the bolaamphiphilic sophorolipid derivatives. ε = reaction yield, CE = carbon efficiency, AE = atom economy.

CE (%) AE (%) ε (%) Peracetylated N,N’-dialkyl bolaamphiphilic SL 19 69 60 11a 19 70 61 11b 24 70 61 11c 16 71 62 11d 32 69 60 11i 31 70 61 11j 15 71 62 11k Peracetylated N-alkyl bolaamphiphilic SL 33 69 60 12a 9 70 61 12b 23 70 61 12c 11 72 63 12d Peracetylated Dicationic bolaamphiphilic SL 18 70 63 14a 18 70 64 14b 23 71 64 14c 15 72 64 14d 32 70 63 14e 31 70 64 14f 15 72 64 14g 15 71 65 14h Peracetylated Monocationc bolaamphiphilic SL 31 69 62 17a 9 70 63 17b 22 71 63 17c 10 73 65 17d

CE (%) AE (%) ε (%) Deprotected N,N’-dialkyl bolaamphiphilic SL 17 44 37 13a 18 45 38 13b 22 46 38 13c 24 48 39 13d

Deprotected N-alkyl bolaamphiphilic SL 33 43 37 16a 8 44 37 16b 19 45 38 16c 11 49 41 16d Deprotected Dicationic bolaamphiphilic SL 18 44 42 15a 18 46 42 15b 18 47 43 15c 14 48 43 15d 29 45 41 15e 28 46 42 15f 15 48 43 15g 14 49 45 15h Deprotected Monocationc bolaamphiphilic SL 25 44 39 18a 8 45 40 18b 21 46 40 18c 10 49 43 18d

Evaluation of the antimicrobial activity The antimicrobial activity of the peracetylated and deprotected N,N’-dialkyl bolaamphiphilic sophorolipids 11 and 13, peracetylated and deprotected dicationic bolaamphiphilic sophorolipids 14 and 15, peracetylated and deprotected N-alkyl bolaamphiphilic sophorolipids 12 and 16, and peracetylated and deprotected monocationic bolaamphiphilic sophorolipids 17 and 18 was evaluated. As reference compounds, the previously synthesized quaternary ammonium sophorolipids 19a and 19b with an octadecyl chain on the nitrogen atom were taken into account (Figure 3).

Figure 3. Quaternary ammonium sophorolipids 19a and 19b having demonstrated high transfection efficiencies36

The Gram-negative bacteria Escherichia coli LMG 8063, Klebsiella pneumoniae LMG 2095 and Pseudomonas aeruginosa PAO1, and the Gram-positive bacteria Staphylococcus aureus ATCC 6538 9 ACS Paragon Plus Environment

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and Staphylococcus aureus Mu50 were chosen as test organisms because of their clinical relevance. This set includes bacteria of high medical concern, some being part of the WHO priority pathogen list for the development of new antibiotic alternatives.48 The bioassay was carried out in 96-well plates in a concentration series ranging from 2500 to 1.22 µg/mL of test compound and approximately 5 x 104 bacteria in a final volume of 200 µL. None of the evaluated compounds inhibited growth of any of the three Gram-negative bacteria tested. A set of nine bolaamphiphilic sophorolipids showed activity against one or both S. aureus strains. For all nine compounds, both the minimal inhibitory concentration (MIC, i.e. the lowest concentration that inhibits growth) and minimal bactericidal concentration (MBC, i.e. the lowest concentration that kills cells) values were determined against the two S. aureus strains (Table S1 ESI). All activities were determined in duplicate or triplicate. Results are considered reproducible when the repetitions don’t differ more than one dilution. In case a higher difference is obtained, the highest value is reported. Within this set of bolaamphiphilic sophorolipid derivatives, the peracetylated dicationic and monocationic congeners 14 and 17 proved to be the most active groups of derivatives. The highest activity was obtained for peracetylated N,N’-dibutyl-N,N’-dimethylethylene bolaamphiphilic sophorolipid diammonium di-iodide 14c with MIC and MBC values against S. aureus ATCC 6538 of 39 and 312 µg/mL (=20 and 158 µM), respectively, and MIC and MBC values against S. aureus Mu50 of 156 and 312 µg/mL (=79 and 158 µM), respectively. For a better comparison of the active bolaamphiphilic derivatives with each other and with the previously synthesized quaternary ammonium sophorolipids, the MIC and MBC values were converted based on their molecular weight (Table 2). On this basis, N,N’-dibutyl-N,N’-dimethylethylene bolaamphiphilic sophorolipid diammonium diodide 14c still has the highest activity among all bolaamphiphilic derivatives. However, these MIC values are still fourfold higher than the ones obtained for the deprotected quaternary ammonium sophorolipids 19 with an octadecyl chain on the nitrogen atom. Table 2. MIC and MBC values (µM) for the active compounds against S. aureus ATCC 6538 and S. aureus Mu50. The best results for the new derivatives are highlighted in bold. For comparison, the results

(µM) S. aureus ATCC 6538 S. aureus Mu50

11b 375 MIC MBC >1501 MIC 1501 MBC 1501

14a 165 330 660 1320

14b 80 321 321 321

14c 20 158 79 158

14d 38 153 153 614

17a 369 1476 185 1476

17b 360 720 360 1440

17c 18d 353 116 >1412 233 176 233 706 >1861

19a 2.18 8.76 4.37 17.5

Antimicrobial activities were also evaluated for the petroselinic acid based peracetylated and deprotected dicationic bolaamphiphilic sophorolipids (Table 3, Table S2 ESI). Low activities were observed against the Gram-negative bacteria, but five of the derivatives showed moderate to high activity against the Gram-positive bacteria. The highest activity was obtained for the peracetylated N,N’-dibutyl,-N,N’-dimethylhexamethylene bolaamphiphilic sophorolipid diammonium di-iodide 14g and N,N,N’,N’-tetrabutylhexamethylene bolaamphiphilic sophorolipid diammonium di-iodide 14h, which are almost in the same range as for the previously synthesized deprotected quaternary ammonium sophorolipids with an octadecyl chain on the nitrogen atom. The natural sophorolipid lactone and acid were also evaluated in the antimicrobial analysis. The activity for oleic acid based sophorolipid acid 2 and lactone 3 are displayed in Table 3. These results illustrate that the chemical modification enables an increase in the antimicrobial activity by a factor 100. In the evaluation, an S. aureus Mu50 strain is included, which is a methicillin-resistant Staphylococcus aureus (MRSA) strain with vancomycin resistance. MIC values for activity of vancomycin and clindamycin against this S. aureus Mu50 were reported to be 8 µg/mL (=6 µM) and 512 µg/mL (=1205 µM), respectively.49 Therefore, the activities obtained with compounds 14g and 14h against both S. aureus strains are reasonably good. However, it should be taken into account that these results 10 ACS Paragon Plus Environment

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are obtained with in vitro testing and that further in vivo testing is necessary to determine the actual antibiotic potential of these compounds. In a next step, toxicity on eukaryotic cells should be evaluated to know whether the antimicrobial activity occurs at a lower concentration. Table 3. MIC and MBC values (µM) for the active compounds against E. coli LMG 8063, K. pneumoniae LMG 2095, S. aureus ATCC 6538 and S. aureus Mu50. ND = not determined.

(µM) E. coli LMG 8063 K. pneumoniae LMG 2095 S. aureus ATCC 6538 S. aureus Mu50

MIC MBC MIC MBC MIC MBC MIC MBC

2 3 14e 14f 14g >1607 >1453 >506 492 472 ND ND ND ND ND >1607 >1453 253 246 236 ND ND ND ND ND >1607 182 63.2 15.3 3.69 ND 363 >506 30.7 7.37 >1607 363 63.2 15.3 7.37 ND 1453 >506 246 14.8

14h 15h 19a 19b 454 >620 >1122 >1071 ND ND ND ND 227 >620 >1122 >1017 ND ND ND ND 3.55 620 2.18 2.09 3.55 ND 8.76 2.09 7.09 310 4.37 4.18 7.09 ND 17.5 16.7

Evaluation of the transfection efficiency Transfection efficiencies for the bolaamphiphilic sophorolipids were evaluated in a similar way as for the previously synthesized quaternary ammonium sophorolipids.36 High transfection results were obtained for the two derivatives having an octadecyl chain on the nitrogen atom (Figure 3). Lipofectamine 3000 (LFM), a commercial lipofection reagent, was used as a reference compound in the transfection experiments. The application of quaternary ammonium derivatives for the compaction and delivery of plasmid DNA (pDNA) and nucleic acids to different cell lines, both in vitro and in vivo, is already widely known.50-54 Renewable based gene delivery vectors are of special interest since they are expected to have an increased biocompatibility.40, 55-57 From the set of thirty-two bolaamphiphilic sophorolipids, three cationic compounds (15b, 15d and 18d) were evaluated for their ability to form supramolecular aggregates in water solution by using the lipid film hydration method. For all liposomal solutions, the size of the particles and their surface charge were determined via Dynamic Light Scattering (DLS) and zeta measurements, respectively. The formation of a homogeneous formulation is a necessary prerequisite for the evaluation of the suitability of quaternary ammonium salts as vectors for gene delivery. The compounds were formulated with or without 1,2-dioleyl-sn-glycero-3-phosphoethanolamine (DOPE). Formulation with DOPE proved to be necessary to obtain homogenous formulations (Table 4), as was previously reported for similar compounds.36 For the monocationic bolaamphiphilic sophorolipid 18d, a positive zeta potential was obtained as is expected for liposomes generated from cationic lipid derivatives. However, for both dicationic bolaamphiphilic sophorolipids 15b and 15d, negative zeta potentials were obtained for formulations both with and without DOPE. Therefore, only compound 18d was considered for further evaluation of the transfection efficiency. Table 4. Size and zeta potential measurements of liposomal solutions prepared at 1.5 mM after 2 days of hydration without (left) or with (right) DOPE. ND = not determined.

15b 15d 18d

Size (nm)

Polydisp. index

Zeta (mV)

Size (nm)

ND 73 ± 11 ND

ND 1 ND

ND -0.39 ND

432 ± 15 175 ± 0.1 103 ± 1

Polydisp. index + DOPE 0.41 0.13 0.28

Zeta (mV) -20.1 -24.1 44

The capacity to compact pDNA was evaluated for the liposomal formulation of compound 18d with DOPE by pDNA retardation assays on agarose gel electrophoresis (Figure 4). This was performed at different charge ratios (CR) in a similar way as for the previously synthesized quaternary ammonium 11 ACS Paragon Plus Environment

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sophorolipids 19a-b. CR is defined as the number of positive charges provided by the cationic lipid derivative divided by the number of negative charges carried by the pDNA. The previously synthesized quaternary ammonium sophorolipids 19a-b show a low capacity to complex pDNA at each CR. A similar behavior was observed for compound 18d.

Figure 4. DNA complexation ability of compound 18d (formulated with DOPE) as determined via the agarose gel retardation assay for different charge ratios. Previously synthesized quaternary ammonium sophorolipids 19a and 19b, naked DNA and LFM (Lipofectamine 3000) were used as controls.

In the transfection assay, compound 18d was evaluated considering its ability to deliver DNA to three human-derived cell lines, namely melanoma cells (SKMEL28) and two airway epithelial cells i.e. (i) lung carcinoma (A549) and (ii) normal bronchial (16HBE) cells. A reporter (luciferase-encoding) pDNA was used that allowed the determination of the transfection efficiency via highly sensitive luminescence measurements. The formulation of compound 18d with DOPE was evaluated at different CR. Compared with the previously synthesized quaternary ammonium sophorolipids 19a-b and the control LFM, compound 18d was almost ineffective for the transfection of the three cell lines (Figure 5). No real toxicity was observed towards any cell line (Figure 6).

Figure 5. Transfection efficacies (TE) of compound 18d formulated with DOPE on three cell lines (A549, 16HBE and SKMEL28) using luciferase-encoding pDNA (DNA). TE are expressed in RLU per mg of proteins (n = 3). Lipofectamine (LFM) and naked (uncomplexed) pDNA were used as positive and negative controls, respectively.

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Figure 6. Cell viability determined 48 h after incubation of the cells with lipoplexes prepared with compound 18d formulated with DOPE. Naked pDNA (DNA) was used as the negative control. Values are expressed as a percentage of the viability determined with untransfected cells.

Evaluation of the self-assembly properties In an attempt to correlate the antimicrobial and transfection data, the self-assembly behavior in water of the bolaamphiphilic sophorolipids was evaluated via Small-Angle X-ray Scattering (SAXS) analysis. All samples were analyzed in milli-Q grade water within 24 h after sample preparation in a broad concentration range (0.78-100 mg/mL). In the following discussion, we only report the SAXS data at 6.25 mg/mL (=3.88-4.79 mM) for the dicationic bolaamphiphilic sophorolipids 15, at 10 mg/mL (=8.32-10.38 mM) for the N-alkyl bolaamphiphilic sophorolipids 16 and at 12.5 mg/mL (=9.30-11.31 mM) for the monocationic bolaamphiphilic sophorolipids 18. The choice to show this set of concentration values is a good compromise between a scattering signal with a satisfactory signal-tonoise ratio and the range of concentrations used in the previous experiments. Figures 7-9 show the SAXS data of only deprotected dicationic bolaamphiphilic sophorolipids 15a-15g, N-alkyl bolaamphiphilic sophorolipids 16a-16d and monocationic sophorolipids 18a-18d, because the peracetylated derivatives are poorly soluble in water. According to the SAXS data, the self-assembly properties of the quaternary ammonium bolaamphihilic sophorolipids are neither uniform nor straightforward to interpret. The group of compounds 15 (a, e-h) shows a poor, although clearly present, scattering signal. A qualitative analysis of the data using a Guinier approach provides a set of radii of gyration (Rg) below 1 nm (Table 4).58 By using the classical Tanford formula lc (Å) = 1.5 + 1.265nc (1) (with lc and nc being the hydrocarbon chain length and the number of carbon atoms in the chain respectively) to estimate the length of the fully extended hydrocarbon chain, one can estimate the approximate overall size of any of these compounds to range between 5 and 6 nm.59 Under these circumstances, the aggregates probed by SAXS must be composed of few, folded, bolaform compounds 15 (a,e-h). The set of compounds 15b-d, of which the Rg is difficult to estimate, seems to be composed of larger self-assembled objects of undefined morphology. The value of the slope in the log(I)-log(q) profiles can help, in some cases, identifying the qualitative nature of the morphology, or the fractional dimension.58 In the present case, compounds 15c and 15d have slopes close to -1 (Table 4), which could indicate the presence of elongated, cylindrical objects although compound 15d has a more complex profile. The value of the slope found for compound 15b (-1.79 ± 0.03) and its poor overall scattering signal do not allow a precise interpretation although values between -1 and -2 have been reported for flexible cylinders.

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Figure 7. SAXS data of oleic acid based dicationic bolaamphiphilic sophorolipids 15a-15d at a concentration of 6.25 mg/mL (=4.33-4.79 mM).

In the family of compounds 16 and 18, one can find strong similarities in the self-assembly behavior. Compounds 16a, 16b, 18a and 18b are all characterized by a scattering profile providing an estimated Rg < 1 and, above all, an important low-q scattering signal indicating the presence of larger objects. For compounds 18a and 18b, the slopes can be evaluated in the vicinity of -1.6 (Table 5), which is a value observed for elongated wormlike micelles.28, 60-62 Compounds 16c and 18c have typical scattering profiles of crystalline flat fibers, characterized by a -2 slope in the low-q range and a diffraction peak, here localized at q = 2.28 nm-1, and indicative of a repeating distance between the bolaform lipid planes of 2π/q = 2.75 nm. Similar data have been reported for several sophorolipidbased systems forming twisted nanoscale fibers.31, 63 Finally, compounds 16d and 18d have the typical profile of spheroidal micelles in the presence of repulsive interactions, which can be recognized by the flattened low-q signal for 16d and the broad interaction peak for 18d. Similar data have been reported for acidic sophorolipids and acidic sophorolipids mixed with sodium dodecyl sulfate.30, 64 As a general comment, irrespective of the mono- and diammonium linker in the bolaform sophorolipids, the nature of the substitute strongly impacts the self-assembly behavior of these molecules. In the case of methyl or butyl groups (compounds 15a-h, 16a-b and 18a-b), the selfassembly properties are rather poor. In some cases, small molecular aggregates are formed in the presence of larger objects of which the morphology is rather unclear without further microscopy studies, which are out of the scope of this work. On the contrary, the octadecyl hydrocarbon group on samples 16d and 18d drives the self-assembly of the bolaform sophorolipids into micelles, most likely through the hydrophobic interaction which is a well-known fact in the theory of the self-assembly of amphiphiles.59, 65-66 Similar results have been observed on several long-chained quaternary ammonium 14 ACS Paragon Plus Environment

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sophorolipids such as compounds 19a and 19b (unpublished results). Intermicellar repulsive interactions characterize these systems and the strength of the interaction depends on the charge localized on the nitrogen. An ammonium group (18d) favors electrostatic interactions (well-defined, broad scattering peak) which were previously described also for sophorolipid systems, while an amino group favors less pronounced, excluded volume, interactions (flattened low-q signal).30, 64 Finally, the presence of an aryl group favors π-π stacking which is known to drive low molecular weight molecules, including bolaform glycolipids, towards the formation of self-assembled fibers.67

Figure 8. SAXS data of petroselinic acid based dicationic bolaamphiphilic sophorolipids 15e-15h at a concentration of 6.25 mg/mL (=3.88-4.50 mM).

Among all non-acetylated molecules, monocationic bolaamphiphilic sophorolipid 18d, having an octadecyl substituent, was the only compound which proved to be interesting for the evaluation of the transfection properties. However, this compound was much less efficient in comparison to the previously synthesized compounds 19a-b, and the reference lipofection reagent Lipofectamine. Interestingly, 18d was the only non-acetylated bolaamphiphilic compound for which antimicrobial activity could actually be observed. Also here, the activity was much lower compared to the previously synthesized compounds 19a-b. Recent work on several long-chained quaternary ammonium sophorolipids, such as compounds 19a and 19b, demonstrates a strong correlation between the antimicrobial or transfection efficiencies and the self-assembly properties of the compounds (submitted results). The explanation is not straightforward for the relationship between self-assembly and transfection efficiency. Different experimental conditions were applied for the measurement of the transfection and self-assembly properties. Self-assembly properties were evaluated with compounds independently solubilized in water, whereas sophorolipids are associated with DOPE (1:1 ratio) to form liposomes that are subsequently mixed with pDNA for the transfection experiments. In consequence, the SAXS results are worth only for the sophorolipid derivatives placed in water but 15 ACS Paragon Plus Environment

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cannot be extended to the liposomal solutions where DOPE likely plays an important role for the supramolecular structuration. Mixtures with DOPE are not included in the self-assembly analysis because of the overlap between the signal of micelles and liposomes in the 0.8 nm-1 < q < -3 nm-1 region. In the current case, self-assembly confirms that compound 18d is better suited for transfection and antimicrobial action with respect to the other non-acetylated compounds. However, if micelle formations seems to be a necessary condition for one of the above-tested non-acetylated compounds to display antimicrobial and transfection properties, it is definitely not sufficient to guarantee a high performance, which can depend on the interaction between the compound and DOPE, for transfection, and between the molecule and the bacterial strain, for the antimicrobial activity. Table 5. Radii of gyration, Rg, low-q slopes and position of diffraction peaks obtained from the SAXS data presented in Figure 7, Figure 8 and Figure 9. Values of Rg have been determined using the Guinier treatment.

15a 15b 15c

Rg (nm)

Slope

0.67

-3.12 ± 0.09 -1.79 ± 0.03 -0.94 ± 0.01 -0.82 ± 0.03 (q> 0.3) -1.40 ± 0.01 (q< 0.15)

15d 15e 15f 15g 15h 16a 16b 16c 16d 18a 18b 18c 18d

Peak (nm-1)

0.67 0.66 0.69 0.82 0.67 0.51 3.80 0.74 0.75

-1.90 ± 0.01

2.28

-1.67 ± 0.01 (q< 0.20) -1.72 ± 0.02 (q< 0.20) -2.04 ± 0.02

2.28

3.20

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Figure 9. A) SAXS data of N-alkyl bolaamphiphilic sophorolipids 16a-16d at a concentration of 10 mg/mL (=8.32-10.38 mM) and pH 7. B) SAXS data of monocationic bolaamphiphilic sophorolipids 18a-18d at a concentration of 12.5 mg/mL (=9.30-11.31 mM).

Conclusions A new set of sophorolipid derivatives, i.e. bolaamphiphilic sophorolipids, was synthesized starting from a sophorolipid aldehyde intermediate via reductive amination with diamines or primary amines. Attempts to introduce octadecyl substituents or an aromatic linker on the diamine bolaamphiphilic sophorolipids were unsuccessful, probably due to the poor solubility of the N,N’-octadecyldiamines and steric hindrance induced by the aromatic linker. The new bolaamphiphilic sophorolipids were evaluated for their antimicrobial, transfection and self-assembly properties. The antimicrobial activity was evaluated against the Gram-positive bacteria S. aureus ATCC 6538 and S. aureus Mu50, and against the Gram-negative bacteria E. coli LMG 8063, K. pneumoniae LMG 2095 and P. aeruginosa PAO1. Fourteen of the derivatives were active against the Gram-positive bacteria tested, while five of them also showed low activity against the Gram-negative bacteria. The peracetylated dicationic and monocationic congeners 14 and 17 proved to be the most active groups of derivatives. The best results were obtained for the peracetylated N,N’-dibutyl-N,N’-dimethylhexamethylene bolaamphiphilic sophorolipid diammonium di-iodide 14g and N,N,N’,N’-tetrabutylhexamethylene bolaamphiphilic sophorolipid diammonium di-iodide 14h, with activities almost in the same range as for the previously synthesized deprotected quaternary ammonium sophorolipids with an octadecyl chain on the nitrogen atom. Transfection efficiencies were evaluated for three cationic bolaamphiphilic sophorolipids, namely 15b, 15c an 18d. Formulation with DOPE was required to obtain homogeneous liposomal solutions and a positive zeta potential (as expected for liposomes with cationic lipid derivatives) was only obtained for the N-octadecyl bolaamphiphilic sophorolipid ammonium iodide 18d. Then, the 17 ACS Paragon Plus Environment

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ability of the liposomal formulation with compound 18d to compact pDNA was assessed. A low compaction was observed for compound 18d, as was also the case for the previously synthesized quaternary ammonium sophorolipids. The capacity of compound 18d to achieve transfection of eukaryotic cells was assessed on three cell lines (A549, 16HBE and SKMEL28). Compared with the previously synthesized quaternary ammonium sophorolipids and the control LFM, compound 18d was almost inefficient for the transfection of the three cell lines and no real toxicity was observed towards any of these cell lines. Evaluation of the self-assembly properties indicated that the presence of an octadecyl chain was necessary for the compounds to self-assemble as micelles. The molecular selfassembly and the net charge of the compounds seem to have an influence on the antimicrobial activity and transfection efficiency. These results are promising to further extend the existing set of bolaamphiphilic sophorolipids and their use for medical and self-assembly applications. Acknowledgements The research leading to these results has received funding from the Long Term Structural Methusalem funding by the Flemish Government (grant number BOF09/01M00409). This work received financial support by the European Synchrotron Radiation Facility (ESRF), Grenoble, France, under the experiment number MX1821. This work benefited from the use of the SasView application, originally developed under NSF award DMR-0520547. SasView contains code developed with funding from the European Union's Horizon 2020 research and innovation programme under the SINE2020 project, grant agreement No 654000. Supporting information An experimental section on the general instrumental methods and general synthesis procedures is included in the supporting information, together with two tables on the antimicrobial activities of the compounds (µg/mL), the characterization of the synthesized compounds and the accompanying 1Hand 13C-NMR spectra. Notes and references 1. Fuhrhop, A. H.; Wang, T. Y., Bolaamphiphiles. Chem Rev 2004, 104 (6), 2901-2937, DOI 10.1021/cr030602b. 2. Benvegnu, T.; Brard, M.; Plusquellec, D., Archaeabacteria bipolar lipid analogues: structure, synthesis and lyotropic properties. Curr Opin Colloid In 2004, 8 (6), 469-479, DOI 10.1016/j.cocis.2004.01.005. 3. Berchel, M.; Le Gall, T.; Lozach, O.; Haelters, J. P.; Montier, T.; Jaffres, P. A., Lipophosphoramidate-based bipolar amphiphiles: their syntheses and transfection properties. Org Biomol Chem 2016, 14 (10), 2846-2853, DOI 10.1039/c5ob02512e. 4. Le Gall, T.; Barbeau, J.; Barrier, S.; Berchel, M.; Lemiegre, L.; Jeftic, J.; Meriadec, C.; Artzner, F.; Gill, D. R.; Hyde, S. C.; Ferec, C.; Lehn, P.; Jaffres, P. A.; Benvegnu, T.; Montier, T., Effects of a Novel Archaeal Tetraether-Based Colipid on the In Vivo Gene Transfer Activity of Two Cationic Amphiphiles. Mol Pharmaceut 2014, 11 (9), 2973-2988, DOI 10.1021/mp4006276. 5. Benvegnu, T.; Rethore, G.; Brard, M.; Richter, W.; Plusquellec, D., Archaeosomes based on novel synthetic tetraether-type lipids for the development of oral delivery systems. Chem Commun 2005, (44), 5536-5538, DOI 10.1039/B511440C. 6. Cornell, B. A.; BraachMaksvytis, V. L. B.; King, L. G.; Osman, P. D. J.; Raguse, B.; Wieczorek, L.; Pace, R. J., A biosensor that uses ion-channel switches. Nature 1997, 387 (6633), 580583, DOI 10.1038/42432. 7. Nuraje, N.; Bai, H. Y.; Su, K., Bolaamphiphilic molecules: Assembly and applications. Prog Polym Sci 2013, 38 (2), 302-343, DOI 10.1016/j.progpolymsci.2012.09.003.

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8. Benvegnu, T.; Plusquellec, D.; Lemiegre, L., Surfactants from renewable sources: Synthesis and applications. In Monomers, polymers and composites from renewable resources, Belgacem, M. N.; Gandini, A., Eds. Elsevier: Oxford, UK, 2008; p 560. 9. Grinberg, S.; Kipnis, N.; Linder, C.; Kolot, V.; Heldman, E., Asymmetric bolaamphiphiles from vernonia oil designed for drug delivery. Eur. J. Lipid Sci. Technol. 2010, 112 (1), 137-151, DOI 10.1002/ejlt.200900107. 10. Ewonkem, M. B.; Grinberg, S.; Lemcoff, G.; Shaubi, E.; Linder, C.; Heldman, E., Newly synthesized bolaamphiphiles from castor oil and their aggregated morphologies for potential use in drug delivery. Tetrahedron 2015, 71 (45), 8557-8571, DOI 10.1016/j.tet.2015.09.031. 11. Shimizu, T.; Masuda, M., Stereochemical effect of even-odd connecting links on supramolecular assemblies made of 1-glucosamide bolaamphiphiles. J Am Chem Soc 1997, 119 (12), 2812-2818, DOI 10.1021/Ja961226y. 12. Guilbot, J.; Benvegnu, T.; Legros, N.; Plusquellec, D.; Dedieu, J. C.; Gulik, A., Efficient synthesis of unsymmetrical bolaamphiphiles for spontaneous formation of vesicles and disks with a transmembrane organization. Langmuir 2001, 17 (3), 613-618, DOI 10.1021/La000892g. 13. Benvegnu, T.; Lecollinet, G.; Guilbot, J.; Roussel, M.; Brard, M.; Plusquellec, D., Novel bolaamphiphiles with saccharide polar headgroups: synthesis and supramolecular self-assembilies. Polym Int 2003, 52 (4), 500-506, DOI 10.1002/pi.1056. 14. Garamus, V. M.; Milkereit, G.; Gerber, S.; Vill, V., Micellar structure of a sugar based bolaamphiphile in pure solution and destabilizing effects in mixtures of glycolipids. Chem Phys Lett 2004, 392 (1-3), 105-109, DOI 10.1016/j.cplett.2004.05.058. 15. Masuda, M.; Shimizu, T., Lipid nanotubes and microtubes: Experimental evidence for unsymmetrical monolayer membrane formation from unsymmetrical bolaamphiphiles. Langmuir 2004, 20 (14), 5969-5977, DOI 10.1021/La049085y. 16. Gerber, S.; Garamus, V. M.; Milkereit, G.; Vill, V., Mixed micelles formed by SDS and a bolaamphiphile with carbohydrate headgroups. Langmuir 2005, 21 (15), 6707-6711, DOI 10.1021/la050439a. 17. Roussel, M.; Lognone, V.; Plusquellec, D.; Benvegnu, T., Monolayer lipid membrane-forming dissymmetrical bolaamphiphiles derived from alginate oligosaccharides. Chem. Commun. 2006, (34), 3622-3624, DOI 10.1039/b607368a. 18. Bize, C.; Garrigues, J. C.; Corbet, J. P.; Rico-Lattes, I.; Blanzat, M., Bioactive Formulations with Sugar-Derived Surfactants: A New Approach for Photoprotection and Controlled Release of Promethazine. Chemphyschem 2013, 14 (6), 1126-1131, DOI 10.1002/cphc.201200932. 19. Shchelik, I. S.; Sebyakin, Y. L., Design and synthesis of an octavalent bolaamphiphile with terminal lactose residues. Russ J Org Chem+ 2015, 51 (12), 1717-1720, DOI 10.1134/S1070428015120088. 20. Zhan, C. L.; Gao, P.; Liu, M. H., Self-assembled helical spherical-nanotubes from an Lglutamic acid based bolaamphiphilic low molecular mass organogelator. Chem. Commun. 2005, (4), 462-464, DOI 10.1039/b413259a. 21. Ambrosi, M.; Fratini, E.; Alfredsson, V.; Ninham, B. W.; Giorgi, R.; Lo Nostro, P.; Baglioni, P., Nanotubes from a vitamin C-based bolaamphiphile. J Am Chem Soc 2006, 128 (22), 7209-7214, DOI 10.1021/ja057730x. 22. Hu, B. B.; Yuan, Y.; Zhou, X. P.; Li, S. M., Synthesis and properties of a novel bolaamphiphile surfactant derived from proline. Chinese Chem Lett 2016, 27 (3), 447-450, DOI 10.1016/j.cclet.2015.12.019. 23. Naess, S. N.; Elgsaeter, A.; Foss, B. J.; Li, B. J.; Sliwka, H. R.; Partali, V.; Melo, T. B.; Naqvi, K. R., Hydrophilic carotenoids: Surface properties and aggregation of crocin as a biosurfactant. Helv Chim Acta 2006, 89 (1), 45-53, DOI 10.1002/hlca.200690011. 24. Van Bogaert, I. N. A.; Saerens, K.; De Muynck, C.; Develter, D.; Soetaert, W.; Vandamme, E. J., Microbial production and application of sophorolipids. Appl Microbiol Biot 2007, 76 (1), 23-34, DOI 10.1007/s00253-007-0988-7. 25. Van Bogaert, I. N. A.; Zhang, J. X.; Soetaert, W., Microbial synthesis of sophorolipids. Process Biochem. 2011, 46 (4), 821-833, DOI 10.1016/j.procbio.2011.01.010.

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For Table of Contents Use Only Table of Contents graphic:

Sophorolipids, renewable biosurfactants, are used for the synthesis of innovative bolaamphiphilic compounds for application in the medical sector.

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