The Chemistry of Amicetin, a New Antibiotic1, 2

CHEMISTRY OF AMICETIN, A NEW ANTIBIOTIC. 5867. [CONTRIBUTION FROM THE LILLY RESEARCH LABORATOREES. AND THE UPJOHN RESEARCH...
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CHEMISTRY OF AMICETIN, A NEWANTIBIOTIC

Dec. 5, 1953 [CONTRIBUTION FROM THE

5867

LILLYRESEARCH LABORATOREES AND THE UPJOHN RESEARCH LABORATORIES]

The Chemistry of Amicetin, a New Antibiotic1i2 BY EDWINH. FLYNN, J. W. HINMAN, E. L. CARON AND D. 0. WOOLF,JR. RECEIVED JULY 20, 1953 The physical and chemical properties of amicetin, a new antitubercular antibiotic, have been studied. It has been shown that cytosine, p-aminobenzoic acid and dextro-a-methylserine are present in the molecule. Degradation of amicetin to cytosamine (IV) and to cytimidine (VI) is described. A structure is postulated for cytimidine and partial structures are derived for cytosamine and amicetin (111).

Amicetin is an antibiotic produced by Streptovinaceusdrappus and by Streptomyces fasciculatis. Fermentation, extraction and purification procedures have been The purpose of this paper is to discuss the chemistry of amicetin and to present a partial structure of the compound. Amicetin is a colorless, crystalline substance insoluble in water and in most organic solvents but readily soluble in l-butanol saturated with water. It is amphoteric and dissolves in dilute acids or bases. Two Crystalline forms of amicetin have been observed, differing in melting point and solubility but having similar microbiological activity. The lower melting, more soluble form has a poorly defined softening point of 165169' and is thought to be a hydrate. The higher melting, less soluble form melts sharply at 244245". The two forms are interconvertible by recrystallization under appropriate conditions (see Experimental). Elemental analyses are in agreement with the formula C Z ~ & ~ N , S (formula O~ weight 620.7). Attempts to establish a molecular weight by the Ra$ method failed because of instability and insolubility in common Rast solvents. The molecular weight was estimated from potentiometric titrations6 to be about 640. Group analyses indicated two N-methyls, at least three C-methyls, one primary amine and no alkoxyl. Amicetin absorbs strongly in 'the ultraviolet, the intensity and position of maximum absorption varying with the pH of the solution. Potentiometric titration of amicetin indicated the presenee of three ionizable groups; one was acidic with a pK'. of 10.4 and two were basic with identical pK', values of 7.0.l A fourth ionizable group with

myces

(1) Presented before the Division of Medicinal Chemistry at the National Meeting of the American Chemical Society at Los Angeles, Cal., March 15-19, 1953. (2) Inquiries may be addressed to Edwin H. Flynn. The Lilly Research Laboratories, Eli Lilly and Company, Indianapolis, Indiana. (3) C. DeBoer, E.L. Caron and J. W. Hinman, THISJOURNAL. I S , 499 (1953). (4) J. W. Hinman, E. L. Caron and C. DeBoer, i b i d , 76, 5964 (1953). (5) M. H. McCormick and M. M. Hoehn, Antibiotics and Chemofheragy, 3, 718 (1953). (6) The methods employed in estimating molecular weights by the use of titration data were described by T. V. Parke and W. W. Davis at the XIIth International Congress of Pure and Applied Chemistry, New York, N. Y., September 12, 1951. (7) The acidic or basic natures of the groups were determined by repeating the titration in 66% dimethylformamidewater. We have observed that, in this solvent system, the PK'. values of acidic groups are generally raised by about 0.5 to 1 pH unit while*#K'. values of basic groups remain unchanged from those obtained when the titration is done in water. The titration of amicetin in 667%dimethylformamide showed one group with a PK'. of 11.1 and two with 9K'.'s of 7.0. The presence of two basic groups with identical fiK's values in the same molecule is a phenomenon we have not observed previously.

PK'. 1.1 was indicated by ultraviolet spectral shifts with changing pH. Amicetin is highly unstable in alkaline solution. Solutions of the compound at pH 8 and above were found to lose microbiological activity at a measurable rate. By contrast, solutions in 0.05 N hydrochloric acid showed no loss of activity after 24 hours. When amicetin was allowed to stand a t room temperature in dilute sodium hydroxide solution, a crystalline precipitate was deposited. This substance, which we call cytosamine, had the composition C18H32N406 according to the elemental analyses and the molecular weight determined by Potentiometric titration. Two basic groups were found, having pK'. values of 3.9 and 7.0. The substance contained one N-methyl group and rapidly decolorized both aqueous bromine and neutral permanganate solutions. Further hydrolysis of cytosamine by heating with 6 N hydrochloric acid led to extensive decomposition. A crystalline hydrochloride was isolated from the mixture and the hydrochloride yielded the crystalline free base on treatment with dilute ammonium hydroxide. This compound, which melted at 310-31201 had the formula C4H6N30. It was identified as cytosine (V). Therefore the cytosamine fragment from amicetin contains cytosine and an unidentified portion, CI~HLNO~. Hydrolysis of amicetin with 6 N hydrochloric acid a t 75-80' gave another product as the hydrochloride. This salt was quite water soluble but yielded a highly insoluble base when treated with sodium bicarbonate. The base, which we call cytimidine, was recrystallized from hot ethanol and melted The formula C I ~ H I ~ N Swas O ~indiat 264-266'. cated by analyses. Complete hydrolysis of cytimidine with boiling 6 N hydrochloric acid yielded three compounds, two of which were isolated by fractional crystallization from the mixture. The least soluble of these had a melting point of 200" and analysis indicated the formula C7H7N02.HCl. It was identified as p-aminobenzoic acid hydrochloride. The second compound obtained was shown to be cytosine hydrochloride. Evaporation of the mother liquor remaining after removal of these substances gave a sirupy product which dissolved readily in ethanol. Adding aniline to the alcohol solution precipitated a crystalline material having the characteristics of an amino acid. It melted with decomposition at 235-240' and was optically active, [.]"D +6.3". A positive ninhydrin reaction was obtained with the substance. Analytical values agreed with the formula C4HsNOn. Titration gave PK'. values of 2.3 and 9.4.

EDWIN H. FLYNN, J. W. HINMAN,E. L. CARONAND D. 0. WOOLF, JR.

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VOl. 75

crystal structure on the infrared absorption. However, there was a definitely greater similarity between DL-Iand the unknown. Potentiometric titration showed DL-Ito have two PK'a values of 2.3 and 9.4. The pKIa values for DL-I1were 2.7 and 9.15. Since the unknown amino acid gave PK'a values of 2.3 and 9.46, it is believed that the amino acid in amicetin is dextro-a-methylserine (I). Cytimidine, C16H1,N6Oa, therefore consists of three components, cytosine, p-aminobenzoic acid and dextro-a-methylserine. The linkages in this portion of the amicetin molecule were deduced from the following facts. Potentiometric titration of cytimidine indicated two ionizable groups, one acidic with a PK', of 9.8 and the second basic with a coz pK', of 6.9. Since no free carboxyl group was CHs 0 CHa 0 present, it was concluded that the carboxyl groups I / / I / / 130-CH2-C-C-OH H2NCHz-C-C-OH of 9-aminobenzoic acid and dextro-a-methylserine I I are involved in amide linkages. Cytimidine gave a 2" OH negative Bratton-Marshall test12showing that the I I1 amino group of p-aminobenzoic acid was bound. reactionls no COz was formed. This fact favors I1 The compound reacted very rapidly with periodate, as the likely structure. However, it has been consuming one mole and forming formaldehyde, reported that a-methyl-a-amino acids behave ammonia and a crystalline product, C I . L H ~ ~ N ~ O ~ abnormally in this reaction.9 To distinguish (VII). On the basis of these facts, VI is advanced between the two possibilities, DL-a-methylserine as the structure for cytimidine. The known properties of amicetin and the struc(I) and DL-a-methylisoserine (11) were synthesized. DL-Iwas prepared by the method of Billman and tural features of cytosamine and cytimidine allow Parker.'O DL-I1was prepared by a slight modifica- the partial structure I11 for amicetin. Cytosine tion of the procedure described by Fourneau." is the only moiety common to both cytimidine These two compounds were compared with the and cytosamine. @-Aminobenzoic acid was not amino acid from amicetin by determination of their present in cytosamine since the Bratton-Marshall dissociation constants and infrared absorption test was negative before and after acid hydrolysis. characteristics. Infrared spectra obtained from If the elements of cytosine and two hydrogen mineral oil mulls were inadequate to rule out either atoms are subtracted from the sum of the formulas of the two possibilities, because of the influence of of cytimidine and cytosamine, the formula for

The presence of a primary amino group was confirmed by the Van Slyke amino nitrogen determination. Direct comparison with threonine, allothreonine and homoserine showed it to be different from these amino acids. A Kuhn-Roth determination gave 0.5 mole of C-methyl, indicating a branched chain. This was confkmed when the reaction with periodate was studied. One mole was consumed very rapidly and two products were isolated from the reaction mixture, as dinitrophenylhydrazones. They were identified as the derivatives of formaldehyde and pyruvic acid. These facts permit two possible structures for the amino acid, I and 11. In the Van Slyke ninhydrin-

I

CiaHzsr'rTOs

0

Y

CHD

lHcl

___)

I SH

\'I1

.~

( 8 ) D. D. Van Slykr, K. 'I. Uillou, U. A. Macb'ddyru and P. Hamil-

ton, J . B i d . Chem., 141, 627 (1941). (9) H. N.Christensen, T. R. Riggs, H. Fischer and I. hl. Palatine, ihid., 196, 2 (1952). (10) J. Billman and E. E . P a r k e r , / l H I s JOURNAL,67, 1069 (1945). Ilr. Billman also kindly furnished a sample prepared by him, for comp:irison purposes. ( I 1) R. Fourneau, Bull. SOL. chiin., [41 6, 229 (I90Y)

amicetin, C29&4N6()8, is obtained. It Seems appar. ent, then, tha! acid hydrolysis cleaved the bond between the cystosine moiety and the uncharacterized portion of the molecule to yield cytimidine. (12) A. C. Bratton and E. K. Marshall, J . Bioi. Chena., 116, 537

(lY3Y).

CHEMISTRY OF AMICETIN, A NEWANTIBIOTIC

Dec. 5, 1953

5869

Alkali hydrolyzed the bond between cytosine and hydroxide for 18 hours the PK'a 10.6 group disp-aminobenzoic acid, yielding cytosamine (IV) appeared and the basic group of 1-methylcytosine These two linkages require further consideration. and the carboxyl of benzoic acid were titratable. A titration of the reaction mixture, obtained by This behavior parallels that of amicetin. mild alkaline hydrolysis of amicetin, showed that The acid-labile bond which is hydrolyzed when the two basic groups (PK'a values of 7) of amicetin cytimidine is formed is still to be considered. were unaffected but the weakly acidic group with Because of its acid lability the three likely points of pKIa 10.4 disappeared. At the same time a new attachment to the cytosine moiety are through the basic group (PIC', 3.8) and acidic group (PK', ring nitrogens or the phenolic hydroxyl a t 2. 3.9) were formed. These were differentiated by R titration in 66% dimethylformamide as well as in water. Cytosamine contained two basic groups with PK', values of 3.9 and 7.0; therefore, the 3 k+/,J6 remainder of the molecule must contain the acidic group and the other basic function. These changes 41 are consistent with a hydrolysis which involves splitting of the cytosine-p-aminobenzoic acid linkage. The acidic, PK', 10.4 group destroyed in the hydrolysis is apparently the "amide" linkage between cytosine and the p-aminobenzoic acid R1 -C- o - N H C - C - CI/H 2 IO H moiety. The degradative changes described above /I I are outlined in the accompanyingdiagram. 0 hH2 Since the acidity of the NH group of the cytosinep-aminobenzoic acid linkage was unexpected, a Attachment at position 3 can be excluded by the suitable model compound was prepared to confirm following information. Cytosamine (IV) was this observation. l-Methyl-2-keto-4-benzamido-treated with nitrous acid to remove the $-amino 1,2-dihydropyrimidine was prepared by the follow- group of the cytosine nucleus. The resulting uracil ing reaction sequence. derivative was then treated with diazomethane, hydrolyzed, and 3-methyluracil was isolated as the H 5-bromo derivative X.

.

3

I

"4

I o\

I \T

H 'N>

1, CHZNZ

O\/S

I

I

0

CH3

IV

I

I N

2"

I

0H

VI11

C - 0 IX

The preparation of 1-methylcytosine (VIII) has been described by Hi1be1-t.l~ The above reaction sequence is simplified and utilizes the observation of Hilbert and Jansen that the 4-alkoxy group will undergo ammonolysis readily.14 Titration of VI11 showed one basic group, pK'. 4.5. However, IX had one weakly acidic group with a PK', of 10.6. When allowed to stand in 0.1 N sodium (13) G. E. Hilbert, THISJOURNAL, 66, 190 (1934). (14) G. E. Hilbert and E. F. Jansen, ibid., 66, 60 (1936). also G E. Hilbert and T. B. Johnson, i b i d . , 52, 4489 (1930).

Cf.

X

This degradative procedure was applied in cytidine and uridine structure studies by Levene and Tipson.ls Attachment a t position 2 would seem to be excluded by two facts. First, cytosamine does not reduce Fehling solution but acid hydrolysis of the substance gives a solution which does. It has been reported that 0-glycosides of 2-hydroxypyrimidines are unstable to base as well as to acid and reduce Fehling solution directly.lC It is also believed that the oxygen in the 2-position must be fixed in the keto form to provide the acidic NH group present in amicetin and in the model compound IX. Support for this belief comes from the fact that N-benzoylcytosine has only one acidic group which is attributed to the phenolic hydroxyl at position 2. It therefore seems most likely that the C14HBNO5 grouping is attached to the 1-position of cytosine. Analogy with the naturally occurring pyrimidine nucleosides cytidine and uridine also favors this view. Studies on the nature of the remainder of the amicetin molecule will be reported a t a later date. Acknowledgment.-The authors are grateful to Messrs. W. L. Brown, H. L. Hunter, W. J. Schenck, W. A. Struck and associates for microanalyses; (15) P. A. Levene and R. S Tipson, J . Biol. Chcm., 104, 385 (1934). (16) P. A. Levene and H. Sobotka. ibid.. 66, 469 (1925).

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EDWINH. FLYNN, J. W. HINMAN, E. L. CARON AND D. 0. WOOLF,JR.

to Messrs. T. V. Parke and J. E. Stafford, Mrs. A. E. Fonkin and Drs. H. Boaz and J. L. Johnson for much of the physical chemical data; and to Dr. R. G. Jones for helpful advice and criticism.

Vol. 75

product weighed 2.34 g . and melted indefinitely at 262265' dec. with some prior sublimation. This hydrochloride was readily converted t o the base by dissolving in 50 ml. of water and adding excess ammonium hydroxide. The precipitated base recrystallized readily from hot water and melted at 310-312" dec. It was identified as cytosine by ExperimentaI'7 direct comparison of its infrared and ultraviolet absorption The amicetin used in these experiments was produced by spectra with those of authentic cytosine. X-Ray patterns fermentation and isolated as described by C. DeBoer, et a1.5 were also identical as were picrate salts prepared from the Crystalline Forms of Amicetin.-Amicetin (0.10 g. of the unknown and cytosine. Cytimidine from Amicetin.-A solution of 3.0 g. of amicelower melting form) was suspended in 0.5 ml. of water and the suspension was warmed on the steam-bath. The solid tin in 70 ml. of 6 N hydrochloric acid was heated to 70' on the dissolved almost immediately and more dense, compact steam-bath. After 5 minutes the solution was removed crystals of the higher melting form began to appear. After from the heat and allowed to stand 10 minutes longer. a few minutes the suspension was filtered while warm, and Crystallization of cytimidine hydrochloride occurred, and the crystals dried in uacuo; 0.070 g. was obtained. Such was completed by keeping the mixture at 0' for 3 hours. preparations had melting points of 243-244' dec.; [ ( Y ] ~ ~ D The product weighed 1.53 g. and melted at 260-264' dec. +98"(c, 0.8in0.05NHCl); +116.5°(c,0.5in0.1 iY HC1). This material was converted to the base by dissolving in 50 When the high melting form of amicetin was allowed to crys- ml. of water and adjusting the solution t o PH 8.3. Cytimitallize from water a t temperatures below 35', long, fine dine precipitated from the solution. The crystalline prodneedles were obtained which melted indefinitely a t 165- uct weighed 1.19 g. and decomposed a t 255-258'. It was 109". This form could be reconverted to the high melting recrystallized from 650 ml. of boiling water and then had a There was no change on form by the procedure described above. Analytical data decomposition point of 262-263'. (on pig-dried samples) and microbiological assays were the further recrystallization. Anal. Calcd. for C l ~ H 1 , N ~ OC, ~ : 54.37; H, 5.17; N, same for both forms. Typical analytical values for amicetin and two of its salts are eiven below. Arnicatin. Calcd. 21.14. Found: C, 54.46; H , 5.32; N, 21.32. Acid Hydrolysis of Cytimidine.-A solution of 5.0 g. of for C28HUX609(mol. wt. i20.7): C, 56.11; H , 7.15; N , 13.54. Found: C, 56.04, 56.54; H, 6.86, 7.29; N, 14.11, cytimidine hydrochloride in 500 ml. of 6 N hydrochloric 14.14. The samples were pig-dried for 3 hours a t 120' in acid was heated under gentle reflux for 15 hours. On cool_ _ vacuo before ana1;sis. ing, the amber colored solution deposited colorless crystals. Amicetin helianthate was prepared by dissolving the free Refrigeration caused an additional amount of the same base in water a t p H 5 with dilute hydrochloric acid, then product to separate. A total of 1.88 g. of this material was adding one mole equivalent of methyl orange dissolved in the obtained and identified as p-aminobenzoic acid hydrochloride minimum amount of water. The precipitate of amicetin on the basis of titration data (pK'.'s in water: 2.33 and helianthate was recrystallized by dissolving in boiling meth- 4.85), ultraviolet and infrared absorption, X-ray diffracanol and adding water to turbidity. In a capillary the tion, paper chromatography and elemental analyses. helianthate melted iudefiriitely a t 200-210" with some Anal. Calcd. for C7HTOpN.HCl: C, 48.30; H, 4.64; N. prior decomposition. 8.07. Found: C, 48.37; H.4.54; X, 8.15. Anal. Calcd. for C3gH14S609.2ClrHlj?i3O~S: C, 55.60; The mother liquor from the crystals was concentrated in H, 6.06; S , 13.65; S, 5.21. Found: C, 55.35, 55.72; H , vacuo to about one-half of its original volume. After re6.38.6.29: S. 13.93. 13.67: S. 5.02. 5.51. frigeration large colorless crystals separated. This material k i c e t i n dihydrochloride was prepared by adding two as isolated melted with decomposition a t about 255-265". mole equivalents of dilute hydrochloric acid t o an aqueous Papergrams indicated that this product was identical with suspension of the free base, then concentrating the solution the crystalline degradation product from cytosamine, to dryness in vacuo. The salt was recrystallized by dis- namely, cytosine hydrochloride. A total of 1.61 g. of this solving 1.9 g. in 10 ml. of methanol, 8 ml. of water and 2 hydrochloride was isolated. The free base was precipitated from aqueous solution upon treatment of the salt with aqueml. of 0.05 N hydrochloric acid. A total of 100 ml. of acetone was added in portions and crystals separated. ous ammonia and was identified as cytosine by comparison When recrystallized in the :ame manner amicetin dihydro- with an authentic sample. chloride melted a t 190-192 . Anal. Calcd. for C&N30: C, 43.24; H, 4.54; IT, Anal. Calcd. for C~gH4rN~09.2HCl:C, 50.21; H, 6.69; 37.80. Found: C, 43.78: H , 4.66; PI', 37.71. N,12.12; C1, 10.22. Found: C, 50.48, 50.40; H, 6.81, The mother liquor from the above described products 6.51; K, 12.49, 12.65; C1, 10.26, 9.54. mas evaporated to a small volume under reduced pressure Cytosamine from Amicetin.-Arnicetin (5.0 g.) was dis- After removing all of the crystalline material (traces of psolved in 125 ml. of 0 10 N sodium hydroxide and the solu- aminobenzoic acid hydrochloride and cytosine hydrochlotion kept a t room temperature for 24 hours. During this ride), the resulting oil was dissolved in 10 ml. of 95% ethtime crystalline cytosamine had deposited. After cooling anol and treated with 30 ml. of 10% aniline in 95% ethanol. a t 5' for 15 hours the cytosamine was removed by filtration The fine, colorless crystals which formed were collected, and washed with two 3-ml. portions of cold water. The washed with ethanol and ether and dried. The yield was 1.21 g., m.p. 235-240' with decomposition; $6.3" product weighed 2.16 g. and softened a t 160-165', then resolidified to a different crystalline form which melted a t (c, 1.0 in water). This ninhydrin-positive material pos260" with decomposition. Cytosamine recrystallized read- sessed the general properties of an amino acid. Elemental analyses indicated it to be isomeric with threonine, but ily from boiling water. infrared studies showed that it was not threonine, allothreoAmZ. Calcd. for C18Hd406: C, 51.00; H , 8.05; N, 14.00. Found: C, 64.11, 54.57; H, 7.61, 7.52; N, 13.76, nine or homoserine. Nitrous acid liberated all of the nitrogen but according to the usual Van Slyke ninhydrin deter13.79. mination there was no cy-amino nitrogen present. Titration Cytosine from Cytosamine.-Cytosamine (12.5 g.) was studies detected a basic group of PK'. 9.4 and an acidic dissolved in 250 mi. of 6 hydrochloric acid and the solu- group of pK', 2.4. A C-methyl determination indicated tion was refluxed for four hours. Extensive decomposition the presence of one terminal methyl group. These data, occurred, and a black iii5oluble material was deposited. together with degradation and model compound studies The solution was decanted from the insoluble residue, and as given below indicated this amino acid to be dextro-e250 rnl. of 1-butanol was added, forming a homogeneous methylserine. solution. When 100 mi. of water was added, the water Anal. Calcd. for C*H&Oa: C, 40.32; H, 7.61; N, phase separated. After three extractions with water in this 11.76; "2, 11.76; C-CHI, 12.6. Found: C, 40.40; manner, the aqueous extracts were combined, giving a light H , 7.59;total N, 11.96; total "2, 11.33; C-CHs, 7.13. brown solution. This was concentrated to a volume of 20 Periodate Oxidation of Cytimidine.-Titration of cyml. under reduced pressure. A crystalline product separated and was removed after several hours at 5'. The timidine with periodate showed that one mole of the reagent was consumed within 10 minutes. Two milliliters of sodium periodate solution (50 mg./ml.) was added to a solu(17) Melting points were determined with a KoRer micro melting tion containing 61 mg. of cytimidine hydrochloride in 10 point apparatus unless otherwise indicated.

Dec. 5, 1953

CHEMISTRY OF AMICETIN, A

ml. of water. After 30'minutes the microcrystalline precipitate which formed was collected, washed with water and dried to yield about 45 mg., m.p. >300' with decomposition. This product was virtually insoluble in dilute hydrochloric acid and formed a yellow 2,44initrophenylhydrazone. The elemental analyses were in good agreement for p yruvyl-fi-amino-aminobenzoylcytosine. Anal. Calcd. for C14H12N404: C, 56.04; H, 4.03; N, 18.68. Found: C, 55.70; H,3.95; N, 18.79. Aqueous dimedon solution was added to the filtrate from the insoluble product and the dimedon derivative of formaldehyde was obtained and identified by melting point.

NEWANTIBIOTIC

5871

I-Methyl-2-ketd-amino-1 ,Z-dihydropyrimidine.-1Methyl-2-keto-4-methoxy-1,2-dihydropyrimidine1~ (3.0 g.) was placed in a pressure bottle and 50 ml. of methanol saturated with ammonia was added. The mixture was heated in a steam-bath for 24 hours, then cooled to 0". The crystalline product weighed 1.65 g. and melted a t 300-302' dec., unchanged by recrystallization from hot water. Hilbert18 has reported a melting point of 303' for this compound.

l-Methyl-2-keto-Q-benzamido-l,2-dihydropyrimidine.-

To 5 ml. of pyridine was added 0.50 g. of 1-methyl-2-keto 4-amino-1,2-dihydropyrimidine. Benzoyl chloride (0.4 ml.) was added, with no apparent reaction. The mixture was heated on the steam-bath for 2 hours but all of the solid Structure of the Amino Acid from Cytimidme did not dissolve. An additional 2.0 ml. of pyridine and 0.2 A. Periodate Oxidation.-The isolated C4 amino acid ml. of benzoyl chloride were added and the mixture was (238.3 mg., 2.0 mal) from cytimidine was dissolved in 20 brought to boiling with an open flame. The solid dissolved. ml. of water and 457 mg. (2.0 mM) of paraperiodic acid After heating for one hour on the steam-bath the mixture (HJOe) was added. The solution was allowed to stand was cooled to 0'. Crystallization occurred. The reaction one-half hour. Previous oxidation experiments had shown mixture was poured onto 20 g. of cracked k e . The crysthe reaction t o be complete in about 5 minutes. A solution talline product was removed and washed with water, giving Recrystallization from of 316.5 mg. (1.0 mM) of barium hydroxide octahydrate in 0.37 g. of solid, m.p. 210-215'. 15 ml. of water was added and the precipitate removed by ethanol raised the melting point to 221-222'. filtering. The solution was diluted to 50 ml. and 25 ml. of Anal. Calcd. for C12HI1K302:C, 62.87; H , 4.84; N, this was distilled to dryness. The distillate was collected 19.33. Found: C,62.79; H,4.79; N, 18.19. in a cooled receiver, then treated with a solution of 100 Reaction of Cytosamine with Nitrous Acid.-Cytosamine, mg. of dinitrophenylhydrazine in 100 ml. of 5% hydro- 10.0 g., was dissolved in 200 ml. of water by adding glacial chloric acid. After 2 hours the crystalline dinitrophenyl- acetic acid to pH 5. Ten grams of sodium nitrite was added hydrazone was removed by filtration. It weighed 25 mg. to the solution, followed by 12.5 ml. of glacial acetic acid and melted a t 160-163' dec. After recrystallization from added dropwise, with stirring. The solution was kept at 2 ml. of ethanol it was compared with an authentic sample room temperature for 4 hours, and then evaporated to dryof formaldehyde 2,4-dinitrophenylhydrazone by X-ray ness under reduced pressure. The residue was extracted diffraction and infrared absorption. The properties of the by adding 50 ml. of ethanol, warming to BO', and then samples were identical. cooling to room temperature. The insoluble fraction was The remainder of the oxidation solution (25 ml.) was discarded. The ethanol extract was concentrated to dryacidified with 5.0 ml. of 0.2 N hydrochloric acid, and then ness under reduced pressure, and the amorphous, cream extracted with ether for 5 hours in a liquid-liquid extraction colored solid was used for reaction with diazomethane as apparatus. The ether extract was added to 100 ml. of the described below. 2,4-dinitrophenylhydrazine reagent, the mixture shaken 3-Methyl-5-bromouracil from Deaminated Cytosamine.vigorously, and then the ether was removed by warming. The prodict obtained from the nitrous acid treatment deThe precipitated 2,4-dinitr;phenylhydrazone weighed 110 scribed above was dissolved in 150 ml. of methanol and a mg. and melted at 219-220 A mixed melting point with solution of diazomethane in methylene chloride was added pyruyic acid 2,4-dinitrophenylhydrazoneshowed no de- in portions until a slight excess remained. The diazomethpression. A comparison of the X-ray pattern and the in- ane was prepared from 10.0 g. of nitrosomethylurea in the frared spectrum of the unknown with those obtained from usual manner. Vigorous reaction occurred as the diazopyruvic acid 2,4-dinitrophenylhydrazoneshowed the com- methane was added to the methanol solution. After one pounds to be identical. hour at room temperature the solvents were removed under DL-or-Methykerine.-DL-a-Methylserine was prepared reduced pressure. The solid was dissolved in 250 ml. of 4 by the method of Billman and Parker.10 It was recrystalN hydrochloric acid and the solution was refluxed for 4 lized from ethanol-water. The compound sublimed slowly hours. The dark solution was taken t o dryness under reon the Kofler block beginning at 220'. The sublimate duced pressure and the residue was triturated with three 50melted a t 268-273" dec. portions of hot benzene. The benzene extract was DLa-Methylisoserine.-DL-cu-Hydroxy-fl-chloroisobuty- ml. evaporated to dryness and the product dissolved in 2 ml. of ric acid," 5.0 g., was dissolved in 100 ml. of concentrated glacial acetic acid. Bromine was added dropwise until the ammonium hydroxide and the solution was heated in a color persisted, then the acetic acid was removed under repressure bottle on the steam-bath for 18 hours. After evap- duced pressure. After three recrystallizations from water oration to dryness in vacuo, the residue was treated with 25 a constant melting point of 228-230' dec. was obtained. ml. of concentrated hydrochloric acid, then excess hydroAnal. Calcd. for CjHbK202Br: Br, 38.95. Found: Br, chloric acid was removed under reduced pressure. The residue was extracted with three 25-ml. portions of ethanol 38.70. These values are in agreement for the expected product, and the insoluble portion was discarded. Aniline ( 5 ml.) was added to the ethanol solution to precipitate DL-a- 3-methyl-5-bromouracil. Johnson and H e y P have reported a melting point of 228-230" for this compound. methylisoserine. This was recrystallized from 80 ml. of water, giving 1.4 g., m.p. 280-285' dec. Two additional INDIANAPOLIS,INDIANA recrystallizations from the water-alcohol mixture gave DLa-methylisoserine melting a t 282-285 ' dec. (18) G. E. Hilbert and T. B. Johnson, THISJOURNAL, I S , 2005 Anal. Calcd. for C4H9K03: N, 11.76. Found: N, (1930). (19) T. B. Johnson and F. W.Heyl, A m . Chcm. J . , 57, 628 (1907). 11.31.

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