The Purification of Horse Heart Cytochrome c by Ion Exchange

In the multi-week experiment, students investigate the preparative scale purification of horse cytochrome c by ion exchange chromatography using a two-wavelength UV-vis purity assay to characterize the chromatographed protein. The experiment emphasiz

The Modern Student Laboratory. Purification of Horse Heart Cyt c by Ion Exchange. An Undergraduate Quantitative Analysis Project. Patricia Ann Mabrouk1.

new experiential learning laboratory project suitable for use in introductory quantitative analysis (1). It provides students with an opportunity to experience ...

In the multi-week experiment, students investigate the preparative scale purification of horse cytochrome c by ion exchange chromatography using a ...

titration and viscosity data which show abnormal reactivities for threeof the four tyrosines of horse heart cytochrome c, and a remarkable stability of this pro-.

Horse heart cytochrome c contains four tyrosyl residues. ... This behavior of the abnormal tyrosines, as well as the high stability to alkaline pH, is unusual in ...

Nov 9, 2016 - Cassatt , J. C.; Marini , C. P. The Kinetics of Oxidation of Reduced ..... Michael W. Mara , Ryan G. Hadt , Marco Eli Reinhard , Thomas Kroll ...

Nov 9, 2016 - Visible circular dichroism, absorption, and fluorescence spectroscopy were used to probe the binding of horse heart ferrocytochrome c to ...

Remote Perturbations in Tertiary Contacts Trigger Ligation of Lysine to the Heme Iron in Cytochrome c. Jie Gu , Dong-Woo Shin , and Ekaterina V. Pletneva.

Jun 1, 1982 - Journal of the American Chemical Society · Advanced Search .... Reduction of ferricenium ion by horse heart ferrocytochrome c.