Subscriber access provided by University of Winnipeg Library
Agricultural and Environmental Chemistry
The response of colonic mucosa-associated microbiota composition, mucosal immune homeostasis, and barrier function to earlylife galactooligosaccharides intervention in suckling piglets Jue Wang, Shiyi Tian, Hu Yu, Jing Wang, and Weiyun Zhu J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b05679 • Publication Date (Web): 18 Dec 2018 Downloaded from http://pubs.acs.org on December 20, 2018
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 47
Journal of Agricultural and Food Chemistry
1
The response of colonic mucosa-associated microbiota composition,
2
mucosal immune homeostasis, and barrier function to early-life
3
galactooligosaccharides intervention in suckling piglets
4
Jue Wang†, Shiyi Tian†, Hu Yu†, Jing Wang*,†and Weiyun Zhu†
5
†
6
Gastrointestinal Nutrition and Animal Health, Laboratory of Gastrointestinal Microbiology, Joint
7
International Research Laboratory of Animal Health and Food Safety, College of Animal Science and
8
Technology, Nanjing Agricultural University, Nanjing 210095, China.
9
Jue Wang, E-mail:
[email protected] 10
Shiyi Tian, E-mail:
[email protected] 11
Hu Yu, E-mail:
[email protected] 12
*Jing Wang, Corresponding author, Tel: +86-25-84395523. Fax: +86-25-84395314. Email:
13
[email protected] 14
Weiyun Zhu, E-mail:
[email protected] National center for International Research on Animal Gut Nutrition, Jiangsu Key Laboratory of
15
16
17
18 1
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
19
20
21
2
ACS Paragon Plus Environment
Page 2 of 47
Page 3 of 47
Journal of Agricultural and Food Chemistry
22
ABSTRACT
23
Suckling piglets were used to investigate the response of colonic
24
mucosa-associated microbiota composition, mucosal immune homeostasis, and
25
barrier function to early-life galactooligosaccharides (GOS) intervention. 10
26
mL 1 g/kg body weight GOS solutions and physiological saline solutions were
27
fed the newborn piglets in GOS group and in the control (CON) group a week
28
time, respectively. Six piglets from each group were euthanized on the day 8
29
and day 21. GOS piglets had a higher abundance of short chain fatty acids
30
(SCFAs) producer such as Prevotella, Barnesiella, Parabacteroides, and
31
Unclassified Porphyromonadaceae in colonic mucosa (P < 0.05). In addition,
32
the total SCFAs level in colonic digesta of GOS piglets increased on day 8 (P
1% in at least one treatment) in the colonic mucosa were selected
282
to statistically investigate the differences in their relative abundance among the
283
groups (Figure 3). The numerical composition of the bacterial community on
284
the genus level was analyzed (Supplementary Table S4 of the Supporting
285
Information). On day 8, the GOS piglets had higher abundance of the dominant
286
taxa Barnesiella and Prevotella in the colonic mucosa compared to the CON
287
piglets (P < 0.05). Moreover, the early-life GOS intervention decreased the
288
abundance of Dorea (P < 0.05), and the abundance of Alloprevotella was
289
tended to decrease (P = 0.074) in GOS piglets. On day 21, GOS piglets had a
290
higher relative abundance of Parabacteroides, Clostridium XI, and 15
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
291
Unclassified Porphyromonadaceae than that of CON piglets (P < 0.05). The
292
abundance of Unclassified Ruminococcaceae (P = 0.073) was tended to
293
increase in GOS group. In addition, compared with the CON piglets, the
294
abundance of Fusobacterium was significantly decreased in GOS piglets (P
