Aug. 20 1956
COMMUNICATIONS TO THE EDITOR
41TX
captide containing the elements of sulfate and allyl drochloric acid a t room temperature was effective, isothiocyanate, and subsequently on the alkaline but we have mostly used a modification of Yamada's hydrolysis of sinigrin to @-D-l-thioglucopyranose.2 assayg for bound hydroxylamine, cleavage in However, I does not accord with the known 3 M sulfuric acid containing 2,4-dinitropheq-lfacts'~~ that , ~ sinigrin and analogs yield on direct hydrazine for 2 hours a t 95-100'. The hydroschemical degradation, not the amines (RNH2) ex- ylamine liberated was identified by paper chropected from I, but nitriles (RCN) and carboxylic matographylO 0.46-0.51 in 7 :3 methanolacids containing the same number of carbon atoms 6 N hydrochloric acid, detected by three specific as the enzymatically formed isothiocyanates (RN- reagents), determined quantitatively by the CsakyYamada methodg or as ferric benzhydroxamate,' l CS). SC~Hii06 R-[ -S=C
