Thermodynamics of the Two Dissociation Steps of N-Tris

for the two dissociation steps of tricine have been determined at ten temperatures by measurement of the emf of cells without liquid junction containi...
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t o establish what is the largest value of TG (slowest internal rotation) that we can measure. We must have T1"INBTIB5 0.8

Then eq 12 yields p

Therefore, measurable

TG

TG

2 0.6 values are restricted t o

5

2TR

(14)

It should also be noted that when the internal motion becomes very fast ( T G