Environ. Sci. Technol. 2010, 44, 1030–1035
Time-Resolved Fluoroimmunoassay as an Advantageous Approach for Highly Efficient Determination of Sulfonamides in Environmental Waters Z H E N Z H A N G , † J I N G - F U L I U , * ,† BING SHAO,‡ AND GUI-BIN JIANG† State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, P.O. Box 2871, Beijing 100085, and Beijing Center for Disease Prevention and Control, Beijing 100013, China
Received July 1, 2009. Revised manuscript received December 8, 2009. Accepted December 10, 2009.
Using monoclonal antibodies labeled with Eu3+ chelates, timeresolved fluoroimmunoassay (TRFIA) methods were developed for the determination of trace sulfamethazine (SMZ), sulfamethoxazole (SMX), and sulfadiazine (SDZ) in environmental waters. Under the optimized conditions, the developed methods offered (i) low detection limits (9.8 ng/L SMZ, 6.1 ng/L SMX, and 5.4 ng/L SDZ, based on 90% inhibition) which were about 1 order of magnitude lower than that of the enzyme-linked immunosorbent assay (ELISA), (ii) high selectivity with no crossreactivity (
