news
Top-down MS picking up speed Although some prefer to focus on the phase [LC] without any tuning whatsotion for the identification and characterforest, others like to start with the ever,” Kelleher says. ization of proteins by using top-down trees. The two MS-based approaches to Merits of the top-down method inMS/MS data). proteomics—bottom-up, in which the clude detection of posttranslational modWith earlier top-down platforms, the proteins of interest are first digested ifications, individually and in combinalargest proteins detectable in discovinto peptides and then separated before tion; high sequence coverage; and better ery mode had been
