J. Agric. Food Chem. 1999, 47, 1597−1602
1597
Toxicological Assessment of Recombinant Xylanase X22 in Wine Y. Pico´,*,† M. Ferna´ndez,† R. Rodrı´guez,† J. Almude´ver,† J. Man˜es,† G. Font,‡ R. Marı´n,§ C. Carda,∇ P. Manzanares,⊥ and D. Ramo´n⊥ Laboratori de Bromatologia, Toxicologia, and Quı´mica Analı´tica, Facultat de Farma`cia, Universitat de Vale`ncia, Av. Vicent Andre´s Estelle´s s/n, 46100 Burjassot, Vale`ncia, Spain, Laboratori de Histopatologia, Facultat de Medicina, Universitat de Vale`ncia, Av. Blasco Iban˜ez 17, 46010 Vale`ncia, Spain, and Departamento de Biotecnologı´a, Instituto de Agroquı´mica y Tecnologı´a de los Alimentos, CSIC, P.O. Box 73, 46100 Burjassot, Vale`ncia, Spain
Toxicological evaluation of xylanase X22 from Aspergillus nidulans expressed in a wine yeast strain was carried out. The safety of the X22 intake was assessed by digestibility, bioinformatic, and mouse short-term repeated dosing studies, although X22 shows resistance to proteolytic degradation in the gastrointestinal system, is a minority protein component (
