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Trace minerals overload induced hepatic oxidative damage and apoptosis in pigs with long-term high level dietary minerals exposure Junning Pu, Gang Tian, Bin Li, Daiwen Chen, Jun He, Ping Zheng, Xiangbing Mao, Jie Yu, Zhiqing Huang, and Bing Yu J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.5b05613 • Publication Date (Web): 01 Feb 2016 Downloaded from http://pubs.acs.org on February 13, 2016
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
Trace minerals additive
The contents of trace minerals in the natural dietary ingredients
Dietary trace minerals Long-term exposure
Liver
Trace minerals overload
Serum ALT AST
Liver structure damage
Liver Oxidative stress
Liver TBARS PCO 8-OHG
Liver ER stress Inflammation Apoptosis
Liver Deranged lipids and glucose metabolism
ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Trace minerals overload induced hepatic oxidative damage and apoptosis in pigs with long-term
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high level dietary minerals exposure
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Junning Pu1, Gang Tian1, Bin Li1, Daiwen Chen1, Jun He1, Ping Zheng1, Xiangbing Mao1, Jie Yu1,
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Zhiqing Huang1, Bing Yu1*
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1
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Agricultural University, 46# Xinkang Road, Yucheng District, Yaan, Sichuan 625014, China
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*Corresponding author: Bing Yu, Institute of Animal Nutrition, Sichuan Agricultural University, 46#
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Xinkang Road, Yucheng District, Yaan, Sichuan 625014, China. Phone: +86 0835-2885106, fax: +86
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0835-2885106, E-mail:
[email protected] Key Laboratory of Animal Disease-Resistance Nutrition, Animal Nutrition Institute, Sichuan
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ACS Paragon Plus Environment
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Journal of Agricultural and Food Chemistry
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ABSTRACT
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The present study investigated the effects of dietary trace minerals (Cu, Fe, Mn and Zn)
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supplemental strategies on liver oxidative stress, endoplasmic reticulum stress, inflammation and
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apoptosis of pigs. A total of 96 DLY (Duroc × Landrace × Yorkshire) piglets were randomly divided
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into four groups: considered or not considered the trace minerals concentrations in basal diet and then
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added to the requirements proposed by NRC (2012) (+B/NR or –B/NR); considered or not
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considered the basal diet’s trace minerals concentrations and then added to the level of commercial
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trace mineral supplement (+B/PL or –B/PL). Pigs fed from 6.5kg to 115kg. Compared with +B/NR
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diets, –B/PL diets increased serum aspartate aminotransferase (AST) and alanine aminotransferase
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(ALT) concentrations (P
