trans-10,cis-12-Conjugated Linoleic Acid Affects Expression of

Oct 11, 2017 - The molecular mechanisms on milk fat depression (MFD) in response to trans-10,cis-12-conjugated linoleic acid (t10,c12-CLA) supplementa...
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Trans-10, cis-12 Conjugated Linoleic Acid Affects Expression of Lipogenic Genes in Mammary Gland of Lactating Dairy Goats Huaiping Shi, tianying zhang, cong li, jianjue wang, jiangtao huang, and zhongyang Li J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b02377 • Publication Date (Web): 11 Oct 2017 Downloaded from http://pubs.acs.org on October 17, 2017

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Journal of Agricultural and Food Chemistry

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Trans-10, cis-12 Conjugated Linoleic Acid Affects Expression of Lipogenic Genes

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in Mammary Gland of Lactating Dairy Goats

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Huaiping Shi, Tianying Zhang, Cong Li, jianjue wang, jiangtao huang, zhongyang Li

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Running title: Conjugated Linoleic Acid blocks milk fat metabolism

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College of Animal Science and Technology, Northwest A&F University, Yangling,

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Shaanxi 712100, China

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Corresponding author. Tel.:+86-29-8709-2102; fax: +86-29-8709-2164

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Email: [email protected];

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[email protected];

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[email protected] ;

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[email protected];

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[email protected];

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[email protected]

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ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry

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ABSTRACT

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The molecular mechanisms on milk fat depression (MFD) in response to trans-10,

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cis-12 conjugated linoleic acid (t10c12-CLA) supplementation in ruminants, were

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elucidated in this research with dairy goats. Thirty two-year-old Xinong Saanen dairy

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goats (40 ± 5 days in milk (DIM)) at peak lactation stage were assigned to a 3×3 Latin

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Square design (14-d treatment period followed with 14-d washout). Three CLA

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treatments included a) Control, fed the basal diet only without CLA supplementation;

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b) orally supplemented with 8 g/d of lipid-encapsulated CLA (low dose; CLA-1); and

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c) 16 g/d of lipid-encapsulated CLA (high dose; CLA-2). Expression levels of fatty

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acid metabolism genes in the mammary tissues were analyzed by RT-qPCR in 3 goats

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on d 1 and the other 3 goats on d 14 in each group after the discontinuation of CLA

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treatment in the third experimental period. Dietary supplementation of CLA led to a

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significant decrease of milk fat compared with the control (P < 0.05). Milk fat

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concentrations in CLA-1 and CLA-2 groups were 2.74% and 2.42% respectively,

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while 2.99% in control group. Decreases in short- and medium-chain fatty acids (