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Agricultural and Environmental Chemistry
Transcriptional activation of anthocyanin biosynthesis in developing fruit of blueberries (Vaccinium corymbosum L.) by preharvest and postharvest UV irradiation Junfeng Yang, Binbin Li, Wenjun Shi, Zhongzhi Gong, Lu Chen, and Zhixia Hou J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.8b03081 • Publication Date (Web): 01 Oct 2018 Downloaded from http://pubs.acs.org on October 6, 2018
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Transcriptional activation of anthocyanin biosynthesis in developing fruit of blueberries
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(Vaccinium corymbosum L.) by preharvest and postharvest UV irradiation
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Junfeng Yang#, Binbin Li#, Wenjun Shi, Zhongzhi Gong, Lu Chen, Zhixia Hou*
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Corresponding author: Zhixia Hou, Key Laboratory for Silviculture and Conservation of Ministry of Education, Research &
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Development Center of Blueberry, Beijing Forestry University, Beijing 100083, China
Junfeng Yang and Binbin Li contributed equally to this work
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Tel: 86-131-2159-5320,
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Fax: 86-010-6233-7098
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Email:
[email protected] 13
Author Contribution： ：
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Junfeng Yang and Zhixia Hou conceived and designed research, analyzed data, as well as wrote the manuscript. Junfeng Yang, Binbin Li,
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Wenjun Shi, Zhongzhi Gong and Lu Chen conducted experiments. All authors read and approved the manuscript.
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Abstract:
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The effect and mechanism of preharvest and postharvest ultraviolet (UV) irradiation on anthocyanin biosynthesis during blueberry
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development were investigated. The results showed that preharvest UV-B,C and postharvest UV-A,B,C irradiation significantly promoted
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anthocyanin biosynthesis and the transcripts of late biosynthetic genes (LBG) VcDFR, VcANS, VcUFGT and VcMYB transcription factor,
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as well as DFR and UFGT activities in anthocyanin pathway in a UV wavelength- and developmental stage-dependent manner. VcMYB
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expression was positively correlated with that of VcANS and VcUFGT, and coincided with anthocyanin biosynthesis respond to the UV
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radiation. Sugar decreased during postharvest but increased during preharvest UV radiation in mature fruit. Our results indicate that
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UV-responsive production of anthocyanins is mainly caused by the activation of anthocyanin downstream pathway genes, which could
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be up-regulated by VcMYB. Furthermore, different potential response mechanisms may exists between preharvest and postharvest UV
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radiation in blueberries, involving a systemic response in living plants and a non-systemic response in postharvest fruit.
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Keywords: anthocyanin biosynthesis; anthocyanin gene expression; enzyme activity; pre- and postharvest UV radiation; MYB
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transcription factor
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1. Introduction
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Increased ultraviolet (UV) radiation has generated concern due to the potential consequences for nearly all aspects of plant growth and
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development. UV radiation is divided into three types according to wavelength: UV-C (
