Transepithelial Transport of YWDHNNPQIR and Its Metabolic Fate

Feb 20, 2017 - College of Food Science and Engineering/Collaborative Innovation ... University of Finance and Economics, Nanjing 210023, P.R. China...
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Transepithelial Transport of YWDHNNPQIR and its Metabolic Fate#with Cytoprotection against Oxidative Stress#in Human Intestinal Caco-2 Cells Feiran Xu, Lifeng Wang, Xingrong Ju, Jing Zhang, Shi Yin, Jiayi Shi, Rong He, and Qiang Yuan J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.6b04731 • Publication Date (Web): 20 Feb 2017 Downloaded from http://pubs.acs.org on February 22, 2017

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Journal of Agricultural and Food Chemistry

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Transepithelial Transport of YWDHNNPQIR and its

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Metabolic Fate, with Cytoprotection against Oxidative

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Stress, in Human Intestinal Caco-2 Cells

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Feiran Xu, Lifeng Wang*, Xingrong Ju, Jing Zhang, Shi Yin, Jiayi Shi, Rong He,

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Qiang Yuan

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(College of Food Science and Engineering/Collaborative Innovation Center for

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Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality

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Control and Processing, Nanjing University of Finance and Economics, Nanjing

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210023, People’s Republic of China)

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Title running header:

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Absorption and Antioxidation of YWDHNNPQIR

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ACS Paragon Plus Environment

Journal of Agricultural and Food Chemistry

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ABSTRACT

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Studies on the antioxidant peptides extracted from foodstuff sources have included

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not only experiments to elucidate the chemical characteristics of them but have also

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investigated their bioavailability and intracellular mechanisms. This study was

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designed to clarify the absorption and antioxidative activity of YWDHNNPQIR

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(named RAP) which derived from rapeseed protein using Caco-2 cells transwell

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model. Results showed that 0.8% RAP (C0=0.2mM, t=90min) could maintain the

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original structure to across the Caco-2 cell monolayers via the intracellular

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transcytosis pathway, and the Papp (drug apparent absorption rate) was (6.6 ± 1.24)

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×10-7 cm/s. Three main fragments (WDHNNPQIR, DHNNPQIR, and YWDHNNPQ)

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and five modified peptides derived from RAP were found in both the apical and

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basolateral side of the Caco-2 cells transwell model. Among these new metabolites,

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WDHNNPQIR had the greatest anti-oxidative activity in Caco-2 cells apart from the

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DPPH assay. With a RAP concentration of 200 µM, there were significant differences

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in four antioxidative indicators (T-AOC, GSH-Px, SOD, and MDA) compared to the

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oxidative stress control (P