Trial of Near-Infrared Reflectance Spectroscopy in a Human Fiber

Timothy J. AndersonYongfeng AiRoger W. JonesRobert S. HoukJay-lin JaneYinsheng ZhaoDiane F. BirtJohn F. McClelland. 2014,333-349. Abstract | Full Text...
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J. Agric. Food Chem. 1994, 42, 735-738

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Trial of Near-Infrared Reflectance Spectroscopy in a Human Fiber Digestibility Study+ Susan B. Fredstrom,* Hans-Joachim G. Jung,lvll James L. Halgerson,ll Cynthia A. Eyden,l and Joanne L. Slavin'tl Department of Food Science and Nutrition and Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, Minnesota 55108, Division of Gastroenterology, Hepatology, and Nutrition, Department of Medicine, Medical School, University of Minnesota, Minneapolis, Minnesota 55455, and Agricultural Research Service, US.Department of Agriculture, St. Paul, Minnesota 55108

A trial of near-infrared reflectance spectroscopy (near-IRS) to predict fecal concentration of dietary fiber (DF) in humans was performed. Fecal samples from 34 persons consuming 6 diets of known DF content were scanned by near-IRS. Diets consisted of a liquid nutrition supplement and quick breads containing either 0 g of fiber, 10 g of wheat bran, 30 g of wheat bran, 10 g of vegetable fiber mix, 30 g of vegetable fiber mix, or 30 g of sugar beet fiber. Neutral detergent fiber (NDF) determinations were made on all fecal samples. An equation to predict NDF concentration was derived from 109 fecal samples using modified partial least-squares analysis. Neutral detergent fiber concentrations predicted from near-IRS data were compared to observed NDF concentrations of 75 other samples to validate the equation. Comparison of observed vs predicted NDF revealed nearly perfect correlation between the two. Calibration and validation of a near-IRS equation generally requires that tradiational chemical analysis be done on only two subsets of samples, thereby reducing the time and effort needed to analyze DF concentrations. This study demonstrates that near-IRS is a viable tool for studying DF digestibility in vivo in humans.

Near-infrared reflectance spectroscopy (near-IRS) is a highly sensitive analytical method that has been used to predict variation in chemical composition of biological samples (Marten et al., 1985). Simply, the method involves mathematical and statistical analysis of the reflectance spectra in the near-infrared region due to hydrogen bonds in the sample. This analysis is compared to traditional chemical analysis of the component of interest to generate a multiple-regression equation. Prediction of the concentration of the component in similar samples that have been scanned by near-IRS can then be made using the regression equation. Chemical analysis need only be done on one subset of samples to obtain the near-IRS prediction equation, also known as the calibration equation, and on another subset to validate the equation. Therefore, labor, time, and materials are conserved in large-scale studies. Development and use of near-IRS have largely occurred in the field of agriculture. This technique has been used for many years for determining forage quality (Marten et al., 1985) and in forecasting feed intake and digestibility in animals (Allison, 1983;Eckman et al., 1983;Lippke and Barton, 1988). Estimates of animal intake and potential weight gain can be made from the content of nutrients of feeds, such as fiber and crude protein, and from in vitro and in vivo digestibility data, all predicted by near-IRS. Quality of foods and pharmaceuticals for humans has been

* Address correspondence to this author at Department of Food Science and Nutrition, University of Minnesota, 1334 Eckles Ave., St. Paul, MN 55108 [telephone (612) 624-7234; fax (612) 625-5272]. t This study was supported by NCI Grant CA46618 and the Minnesota Agricultural Experiment Station (18-64). Division of Gastroenterology, Hepatology, and Nutrition. 8 Agricultural Research Service. 11 Department of Agronomy and Plant Genetics. 1 Department of Food Science and Nutrition. 0021-8561/94/1442-0735$04.50/0

studied by this method (Lanza, 1983;Osborne et al., 1984; Williams and Sobering, 1986; Williams et al., 1991; Corti and Dreassi, 1993), and it has had limited applications in clinical practice (Koumantakis and Radcliff, 1987; van Toorenenbergen et al., 1988; Benini et al., 1992). Consumption of dietary fiber (DF) is known to result in a myriad of physiologic effects, some of which are the result of fermentation of the DF by colonicbacteria (Pilch, 1987). While in vitro fermentation using human fecal innoculum can be studied, the in vivo fermentation process is affected by the transit time through the colon, the nature of the bacterial flora of the host, and the viscosity and pH of the digesta (Robertson, 1988). Thus, predicting the in vivo digestibility of a given DF is difficult, but important in ascertaining the possible consequences of its consumption. Traditional chemical methods of studying digestibility, where diet and fecal fiber content must be determined for all subjects, are tedious and expensive for large study groups. A more rapid but cost-effective and reliable method would allow more comprehensive studies of DF digestibility in vivo in humans. Given the success of near-IRS analysis in animal nutrition, it seems that the method may have utility in human studies. To study this possibility, fecal samples were examined from a study of human subjects consuming diets of known DF content with the objective of testing the ability of near-IRS to accurately predict concentrations of DF in feces. Near-infrared reflectance spectroscopy was used to predict neutral detergent fiber (NDF) concentrations in these samples, on the basis of a calibration equation derived from analysis of a selected subset of samples. Predicted NDF concentrations were compared to NDF determinations made in the usual fashion on the remaining samples to validate the equation. MATERIALS AND METHODS Thirty-fourhealthy volunteersparticipated in a fiber-feeding study (16 women, 18 men, ages 19-50 years). The design of the study was approved by the Committee on Use of Human Subjects 0 1994 American Chemical Society

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Table 1. Dietary Fiber Content of Diets, as Total Dietary Fiber (TDF) and Neutral Detergent Fiber (NDF), and NDF Content of Fecal Material from Human Subjects fiber fiber content NDF concn of feces ( % ) (g/day) level diet (g/day) TDF NDF n mean SD min max 2.1 3.1 34 6.6 f3.5 1.6 17.1 0 g of fiber 11.0 11.4 34 27.1 f4.1 20.3 35.3 WB 10 29.9 29.7 34 42.0 *4.2 32.8 51.6 30 14.3 9.9 33 29.2 f6.4 8.5 41.5 VF 10 36.5 26.4 34 44.9 110.4 3.4 55.8 30 30.5 19.5 15 21.8 f10.9 10.9 43.3 SBF 30 in Research at the University of Minnesota. The fiber-feeding experimental protocol and diets were previously described (Lampe et al., 1991). Briefly, subjects consumed five diets in assigned random order, each consisting of a liquid nutrition supplement (Resource,Sandoz Nutrition Inc., Minneapolis, MN) and quick breads containing 0 g of added fiber, 10 g of dietary fiber as wheat bran (10 g of WB), 30 g of dietary fiber as wheat bran (30 g of WB), 10 g of dietary fiber as mixed vegetable fiber (62% pea fiber, 33% soy polysaccharide, and 5% orange pectin) (10 g of VF) or 30 g of dietary fiber as mixed vegetable fiber (30 g of VF). During the study, a sixth feeding period was added in which 15 subjects participated. The quick bread in this period contained 30 g of dietary fiber as sugar beet fiber (SBF). (Fiber suppliers were American Association of Cereal Chemists,St. Paul, MN, for WB; Food Products, Inc., Minneapolis, MN, for Fiberich pea fiber; Protein Technologies International, St. Louis, MO, for Fibrim 1450soy polysaccharide;Hercules, Inc., Wilmington, DE, for pectin; and American Crystal Sugar Co., Moorhead, MN, for SBF.) Fiber content of breads, as determined in our laboratory using the Association of Official Analytical Chemists method for total dietary fiber (TDF) analysis (Baglien, 1991) and using the NDF method (Robertaon and Van Soest, 1977), is summarized in Table 1. Subject characteristics and macronutrient content of breads have been previously described (Lampe et al., 1991). Feeding periods were 23 days each, separated by wash-out periods of 2-4 weeks during which subjects consumed their habitual diets. All stools were individually collected and frozen. Feces collected from days 14-19 were used for fiber analysis. Feces were composited and lyophilized. Well-mixed portions of lyophilized samples were ground to pass a 0.5-mm screen (Tecator Cyclotech mill, Model 1093, Tec-tron, Inc., Herndon, VA). Samples of each quick bread were also lyophilized, composited, and ground, as were the raw starch and fiber sources. The raw ingredients included rice flour (the major component of all quick breads), wheat bran, pea fiber, pectin, soy polysaccharide, and sugar beet fiber. All samples were analyzed for NDF using the method of Robertson and Van Soest (1977). Briefly, 0.5-g samples were refluxed with 100mL of neutral detergent, 0.5 g of sodium sulfite (AldrichChemicalCo.,Milwaukee,WI),andO.l mLofheatstable amylase (SigmaChemicalCo., St. Louis MO) for 1h. The solution was then filtered through Gooch crucibles using glass filters (Whatman International Ltd., Maidstone, England, GF/D grade) as a prefilter. Residues were washed twice with hot distilled water, followed by two washes of acetone, and dried for 16-18 h a t 100 OC. Analyses were done in duplicate. When differences between NDF concentrations in the two replicates were greater than two percentage units, the procedure was repeated. Dry matter of samples was determined by drying at 100 O C for 16-24 h. Neutral detergent fiber was expressed as percent of sample dry matter. The average NDF concentration of each sample and replicate was used as the input for the calibration equation. All fecal samples were placed in plastic bottles and tumbled in a rotating plastic drum for approximately 15 min to ensure a uniform subsample. A total of 184 samples were scanned by near-IRS (Pacific Scientific Instrument Div., Model 6250, Silver Spring, MD), and the signal was recorded as log(l/reflectance). Analysis of the spectra and development of the prediction equation were done according to the methods of Shenk and Westerhaus (1991a,b) using InfraSoft International (Port Matilda, PA) analytical software. Spectra from all samples were analyzed by the CENTER program, and no outliers were found.

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NIRS Prediction (% NDF) Figure 1. Plot of near-IRS predicted and observed NDF concentrations for fecal samples of subjects consuming various fiber sources. These samples were not included in the calibration set for developments of the near-IRS prediction equation. The diagonal line represents unity. Table 2. Linear Regression Relationships for Predicted Near-IRS NDF Concentrations vs Observed Chemical Analysis of Validation Set Fecal Samples sample diet no. slope intercept R2 probability 0 g of fiber 8 1.23 f 0.55 1.58 f 2.25 0.46 X0.07 WB 39 1.11f 0.04 -3.43 f 1.51 0.95