Ultra-Sensitive MicroRNA Assay via Surface Plasmon Resonance

Sep 5, 2017 - Quantification of trace serum circulate microRNAs is extremely important in clinical diagnosis but remains a great challenge. Herein we ...
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Ultra-Sensitive MicroRNA Assay via Surface Plasmon Resonance Responses of Au@Ag Nanorods Etching Yu Gu, Juan Song, Mei-Xing Li, Tingting Zhang, Wei Zhao, Jing-Juan Xu, Maili Liu, and Hong-Yuan Chen Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.7b02920 • Publication Date (Web): 05 Sep 2017 Downloaded from http://pubs.acs.org on September 5, 2017

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Analytical Chemistry

Ultra-Sensitive MicroRNA Assay via Surface Plasmon Resonance Responses of Au@Ag Nanorods Etching Yu Gu1, Juan Song1, Mei-Xing Li1, Ting-Ting Zhang1, Wei Zhao1*, Jing-Juan Xu1*, Maili Liu2 and Hong-Yuan Chen1

1: State Key Laboratory of Analytical Chemistry for Life Science and Collaborative Innovation Center of Chemistry for Life Sciences, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, China.

2: State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, Wuhan Centre for Magnetic Resonance, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan, 430071, China.

* Corresponding author. Tel/Fax: +86-25-89687294; E-mail address: [email protected] (W. Zhao) [email protected] (J.J. Xu)

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Analytical Chemistry

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ABSTRACT:

Quantification of trace serum circulate microRNAs is extremely important in clinical diagnosis but remains a great challenge. Herein we developed an ultra-sensitive platform for microRNA 141 (miR-141) detection based on a silver coated gold nanorods (Au@Ag NRs) etching process accompanied by surface plasmon resonance (SPR) shift. Both SPR absorption and scattering responses were monitored. Combined amplification cascades of catalyzed hairpin assembly (CHA) and hybridization chain reaction (HCR) with the sensitive SPR responses of plasmonic Au@Ag NRs, the proposed bioassay exhibited ultra-high sensitivity toward miRNA-141 with dynamic range from 5.0 ×10-17 M to 1.0 ×10-11 M. With target concentration higher than 1.0 ×10-13 M, the color of the solution changed obviously that could be observed with naked eyes. Under dark-field microscopy observation of individual particle, a limit of detection down to 50 aM could be achieved. Owing to the superior sensitivity and selectivity, the proposed method was applied to detecting trace microRNA in serum. Similar SPR assays could be developed simply by re-designing the switching aptamer for the detections of other microRNAs or targets such as small molecule, DNA or protein. Considering the convenient operation, good performance and simple observation modes of this method, it may have great potential in trace bioanalysis for clinical applications.

KEYWORDS: MicroRNA assay, Surface plasmon resonance, Au@Ag NRs, Etching, CHA-HCR

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Analytical Chemistry

INTRODUCTION MicroRNAs (miRNAs), as a short noncoding molecules with 19-23 nucleotides in size, play important roles in gene expression, cell cycle and biology development, etc1,2. In the past, most relative researches focused on intracellular miRNAs, since it was considered to be easily degraded by endogenous RNase in tissue fluid3,4. However, latest research shows that miRNAs released from cancer cells could be present in human plasma in a remarkably stable form5 named circulating miRNAs, which may become a new branch of biomarkers in early tumor diagnosis and clinical management6-8. However, miRNAs assays in plasma or serum are challenging because of their short sequences, high degree of sequence similarity, and ultra-low abundance

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