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J. Agric. Food Chem. 2000, 48, 344−353
Ultrafast Capillary Electrophoretic Analysis of Cereal Storage Proteins and Its Applications to Protein Characterization and Cultivar Differentiation† S. R. Bean‡ and G. L. Lookhart*,§ Department of Grain Science and Industry, Kansas State University, Manhattan, Kansas 66506, and Grain Marketing and Production Research Center, Agricultural Research Service, U.S. Department of Agriculture, Manhattan, Kansas 66502
Free zone capillary electrophoresis conditions have been improved to allow rapid (2-8 min) separations of grain proteins from several cereals (wheat, oats, rice, barley, and rye) with high resolution and reproducibility. This new method utilized the isoelectric compound iminodiacetic acid (IDA) in conjunction with 20% acetonitrile and 0.05% hydroxypropylmethylcellulose. Cultivars of all cereals tested could be differentiated in 3 min, including wheat, using either prolamin or glutelin protein patterns. Resolution was similar to or higher than that of separations in other acidic buffers. Migration time repeatability was excellent with run-to-run variability
