Subscriber access provided by Universitaetsbibliothek | Johann Christian Senckenberg
Article
Uptake and depuration kinetics influence microplastic bioaccumulation and toxicity in Antarctic krill (Euphausia superba) Amanda Dawson, Wilhelmina Huston, So Kawaguchi, Catherine King, Roger Cropp, Seanan Wild, Pascale Eisenmann, Kathy Townsend, and Susan Bengtson Nash Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.7b05759 • Publication Date (Web): 03 Feb 2018 Downloaded from http://pubs.acs.org on February 4, 2018
Just Accepted “Just Accepted” manuscripts have been peer-reviewed and accepted for publication. They are posted online prior to technical editing, formatting for publication and author proofing. The American Chemical Society provides “Just Accepted” as a service to the research community to expedite the dissemination of scientific material as soon as possible after acceptance. “Just Accepted” manuscripts appear in full in PDF format accompanied by an HTML abstract. “Just Accepted” manuscripts have been fully peer reviewed, but should not be considered the official version of record. They are citable by the Digital Object Identifier (DOI®). “Just Accepted” is an optional service offered to authors. Therefore, the “Just Accepted” Web site may not include all articles that will be published in the journal. After a manuscript is technically edited and formatted, it will be removed from the “Just Accepted” Web site and published as an ASAP article. Note that technical editing may introduce minor changes to the manuscript text and/or graphics which could affect content, and all legal disclaimers and ethical guidelines that apply to the journal pertain. ACS cannot be held responsible for errors or consequences arising from the use of information contained in these “Just Accepted” manuscripts.
Environmental Science & Technology is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
Page 1 of 15
Environmental Science & Technology
1 2 3 4 5 6
Uptake and depuration kinetics influence microplastic bioaccumulation and toxicity in Antarctic krill (Euphausia superba) Amanda Dawson*1, Wilhelmina Huston2, So Kawaguchi3, Catherine King3, Roger Cropp1, Seanan Wild1, Pascale Eisenmann1, Kathy Townsend4, Susan Bengtson Nash1
7 8
1. Environmental Futures Research Institute, Southern Ocean Persistent Organic Pollutants
9
Program (SOPOPP), Griffith School of Environment, Griffith University, 170 Kessels Road,
10 11 12 13 14 15 16
Nathan, QLD 4111, Australia 2. School of Life Sciences, Faculty of Science, University of Technology Sydney, 15 Broadway Ultimo, NSW 2007, Australia 3. Australian Antarctic Division, Department of the Environment and Energy, 203 Channel Highway, Kingston, TAS 7050, Australia 4. School of Biological Sciences, Moreton Bay Research Station, University of Queensland, North Stradbroke Island, QLD 4183, Australia
17 18 19 20 21 22
*Corresponding Author
23
Griffith University, 170 Kessels Road, Nathan, QLD 4111, Australia.
24
Telephone +61 0402550085
25
Email address:
[email protected] 26 27
ACS Paragon Plus Environment
Environmental Science & Technology
28
TOC Artwork
29 30
Abstract
31
The discarding of plastic products has led to the ubiquitous occurrence of microplastic particles in the
32
marine environment. The uptake and depuration kinetics of ingested microplastics for many marine
33
species still remain unknown despite its importance for understanding bioaccumulation potential to
34
higher trophic level consumers. In this study, Antarctic krill (Euphausia superba) were exposed to
35
polyethylene microplastics in order to quantify acute toxicity and ingestion kinetics, providing insight
36
into the bioaccumulation potential of microplastics at the first-order consumer level. In the 10 day
37
acute toxicity assay, no mortality or dose dependant weight loss occurred in exposed krill, at any of
38
the exposure concentrations (0, 10, 20, 40, or 80 % plastic diet). Krill exposed to a 20% plastic diet
39
for 24 hours displayed fast uptake (22 ng mg-1 h-1) and depuration (0.22 h-1) rates, but plastic uptake
40
did not reach steady state. Efficient elimination also resulted in no bioaccumulation over an extended
41
25 day assay, with most individuals completely eliminating their microplastic burden in less than 5
42
days post exposure. Our results support recent findings of limited acute toxicity of ingested
43
microplastics at this trophic level, and suggest sublethal chronic endpoints should be the focus of
44
further ecotoxicological investigation.
45
Introduction
46
Plastic has become an integral part of modern daily life throughout, but its low production cost has led
47
to many plastic products to be considered as single use. The short useful-life of plastic products, in
48
combination with their recalcitrant long environmental persistence has led to the ubiquitous
49
occurrence of microplastic particles in the marine environment. The routine monitoring of marine
50
microplastics (defined as plastics
