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Visualization and Identification of Neurotransmitters in Crustacean Brain via Multifaceted Mass Spectrometric Approaches Qinjingwen Cao, Yijia Wang, Bingming Chen, Fengfei Ma, Ling Hao, Gongyu Li, Chuanzi Ouyang, and Lingjun Li ACS Chem. Neurosci., Just Accepted Manuscript • DOI: 10.1021/acschemneuro.8b00730 • Publication Date (Web): 05 Feb 2019 Downloaded from http://pubs.acs.org on February 9, 2019
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ACS Chemical Neuroscience
Visualization and Identification of Neurotransmitters in Crustacean Brain via Multifaceted Mass Spectrometric Approaches Qinjingwen Cao1, Yijia Wang2, Bingming Chen3, Fengfei Ma3, Ling Hao3, Gongyu Li3, Chuanzi Ouyang1, Lingjun Li1,3* 1Department
of Chemistry, University of Wisconsin-Madison, 1101 University Avenue, Madison,
Wisconsin, 53706, United States 2Department
of Chemical Physics, School of Chemistry and Materials Science, University of
Science and Technology of China, 96 Jinzhai Road, Hefei, Anhui, 230026, China 3School
of Pharmacy, University of Wisconsin-Madison, 777 Highland Avenue, Madison,
Wisconsin, 53705, United States
*Corresponding author Tel.: (608) 265-8491 Fax: (608) 262-5345 E-mail:
[email protected] Keywords: MALDI imaging, LC-MS/MS, neurotransmitter, amine metabolites, crustacean, ontissue derivatization
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Abstract Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry imaging (MSI) has emerged as a label-free analytical tool for fast biomolecule profiling on tissue sections. Among various functional molecules, mapping neurotransmitters and related metabolites is of tremendous significance, as these compounds are critical to signaling in the central nervous system. Here, we demonstrated the use of both derivatization and reaction-free approaches that greatly reduced signal complexity and thus enabled complementary signaling molecule visualization on crab brain sections via MALDI-LTQ-Orbitrap XL platform. Pyrylium salt served as a primary amine derivatization reagent and produced prominent signal enhancement of multiple neurotransmitters, including dopamine, serotonin, γ-aminobutyric acid and histamine that were not detected in underivatized tissues. Molecules with other functional groups, such as acetylcholine and phosphocholine, were directly imaged after matrix application. The identities of discovered neurotransmitters were verified by standards using LC-MS/MS. This study broadens our understanding of metabolic signaling in the crustacean nervous system and highlights potential of multifaceted MS techniques for unambiguous neurotransmitter characterization.
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ACS Chemical Neuroscience
Introduction Neurotransmitters and related low molecular weight metabolites (MW
