Volatile constituents of guava fruits (Psidium ... - ACS Publications

Characterization of the Aroma-Active Compounds in Pink Guava (Psidium guajava, L.) by Application of the Aroma Extract Dilution Analysis. Martin Stein...
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J. Agric. Food Chem. 1989, 37, 139-142

Safe, S.; Safe, L.; Mullin, M. PCBs: Congener-specificanalysis of a commercial mixture and a human milk extract. J . Agric. Food Chem. 1985,33, 24-29. Schwartz, T. R.; Stalling, D. L.; Rice, C. L. Are PCB residues adequately described by Aroclor mixture equivalents? Isomer-specific principal components analysis of such residues in fish and turtles. Enuiron. Sci. Technol. 1987, 21, 72-76.

139

Sissons, D.; Welti, D. J. Structural identification of PCBs in commercial mixtures by gas chromatography. J. Chromatogr. 1971, 60, 15-32.

Webb, R. G.; McCall, A. C. Identities of PCB isomers in Aroclors. JAOAC 1972,55,746-752.

Received for review February 8, 1988. Accepted June 21, 1988.

Volatile Constituents of Guava Fruits (Psidium guajava L.) and Canned Puree Osamu Nishimura, Kenji Yamaguchi, Satoru Mihara, and Takayuki Shibamoto*

Essences of fresh guava (Psidium guujavu L.) with white and pink flesh, respectively, were obtained by direct extraction of flesh juices with dichloromethane. Commercial canned puree of guava was water distilled, and the distillate was extracted with dichloromethane. The three essences were analyzed by fused silica capillary gas chromatography and gas chromatography/mass spectroscopy. A total of 122 volatile components were identified: 13 aldehydes, 17 ketones, 31 alcohols, 10 acids, 28 esters, 10 hydrocarbons, and 13 miscellaneous compounds. Quantitatively, the major constituents of fresh fruits were c6 compounds. The total amount of c6 aldehydes, alcohols, and acids comprised 20% of the essence of fresh white and 44% of the essence of fresh pink. The canned puree contained acetoin, which comprised 81% of the essence, as the major constituent.

The guava (Psidium guujaua L.), which has an unique quince- and banana-like odor, is native to Central America. It was distributed into other parts of tropical and subtropical areas such as Asia, South Africa, Egypt, and Brazil by the early 17th century and is now cultivated in nearly 60 countries. The production of guava in the world is still much less than those of other major tropical fruits, but it is economically important in certain countries. In addition to consumption as fresh fruit, guava has been processed into many different foods: jellies, jams, cheese, ketchup, puree, juice powder, nectar, and juices. A pioneer study on volatile components in guava was done in the early 1960s (Kunishi and Seale, 1961). The aroma constituents of guava were not, however, reported until Stevens et al. (1970) identified 22 aroma components of Hawaiian guava. They suggested that 8-ionone, which has a low odor threshold and intense violet aroma, contributed floral flavor to the fruit. Wilson and Shaw (197% who identified 12 terpenes in an extract of guava puree, described that 8-caryophylleneplays an important role in guava aroma. Later, MacLeod and de Troconis (1982) reported that 2-methylpropyl acetate, hexyl acetate, and benzaldehyde had a guava-like odor among 40 volatiles identified in guava from Venezuela. Most recently, Idstein and Schreier (1985) identified 154 compounds in guava from Brazil including 115 compounds described for the first time. In the present study, volatile components of fresh guava fruit from Amami Island, Japan, and canned guava puree from South Africa were identified. D e p a r t m e n t of Environmental Toxicology, University of California, Davis, California 95616 (T.S.), Ogawa & Company, Ltd., 6-32-9 Akabanenish, Kitaku,Tokyo, Japan (O.N., S.M.), and Yokogawa Electric Corporation, 2-9-32 Nakacho, Musashinoshi, Tokyo, Japan (K.Y.). 0021-8561/89/1437-0139$01.50/0

EXPERIMENTAL SECTION Materials. Fresh ripe guava fruits (pink and white) were obtained from Amami Island located 300 miles south of the mainland of Japan. Canned guava puree was purchased from the Potona Canning and Production Co., Ltd. Authentic chemicals for gas chromatographicanalysis were purchased from reliable commercial sources or were donated by Ogawa & Co., Ltd., Tokyo, Japan. Sample Preparations. Fresh guava with white flesh (white) and fresh guava with pink flesh (pink) were treated and analyzed separately. The fruit flesh (1.5 kg) was separated from peel and seeds, sliced into small pieces (3 cm), and homogenized with an electric blender. The homogenized sample was mixed with 1.5 L of deionized water, and stone cells were removed with a centrifuge. The fresh juice (2 L) was extracted with 150 mL of dichloromethane with use of a liquid-liquid continuous extractor for 12 h. After the extract was dried over anhydrous sodium sulfate, the solvent was removed on a Vigreux column at 42 "C. The solvent was further removed with a purified nitrogen stream, and approximately 0.3 mL of a fresh fruit essence was obtained. Canned puree (1.5 kg) was also homogenized with 1.5 L of deionized water and was water-distilled under reduced pressure (40mmHg) in a nitrogen stream at 40 "C. The distillation was continued until 1.5 L of distillate was obtained. The distillate was extracted with 150 mL of dichloromethane with use of a liquid-liquid continuous extractor for 12 h. The extract was treated in the same manner as the one from fresh fruit, and approximately 0.5 mL of essence was obtained. Analysis of Volatiles. Identification of volatile constituents of the guava samples was made by comparison of their Kovats gas chromatographic retention indices and mass spectra to those of authentic compounds. 0 1989 American Chemical Society

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J. Agr‘ic. Food Chem., Vol. 37,No. 1, 1989

Nishimura et

Table I. Volatile Compounds Identified in Guava GC peak area,a % Ik

(CW20M) white pink acetaldehyde hexanal 4-pentenal (E)-3-hexenal (2)-3-hexenal (Z))-a-hexenal (E)-2-hexenal

690 1084 1134 1138 1142 1203 1222

b 3.03 b 0.39 1.04 0.11 3.56

acetone 3-pentanone 2,3-butanedione 2,4-dimethyl-3-pentanone 2-acetylfuran 2-propionylfuran

C

b

0.91

0.36

C C

C

C

C

methyl benzyl ketone 5-ethyl- 2 (5H) furanone

820 958 961 997 1475 1565 1584 1637 1716

isobutyl alcohol isopentyl alcohol pentanol acetoin acetol cyclopentanol hexanol (E)-a-hexenol (2)-3-hexenol (E)-2-hexenol 2,3-butanediol octanol linalool 2-decanol menthol furfuryl alcohol

1048 1180 1230 1255 1266 1283 1323 1334 1362 1388 1494 1523 1529 1613 1619 1623

acetic acid isobutanoic acid butanoic acid isopentanoic acid 2-ethylbutanoic acid

0.14 6.71 0.20 2.11 7.83 0.56 16.86

C

puree Aldehvdes b furfural c (EJ3)-2,4-heptadienal c benzaldehyde c 5-methylfurfural c m-hydroxybenzaldehyde c cinnamic aldehyde Ketones c p-methylacetophenone c furfuryl pentyl ketone 0.10 furfuryl hexyl ketone b &ionone b 2,5-dimethyl-4-hydroxyb 3(2H)-furanone c dihydromethylionone b 5,6-epoxy-@-ionone c p-methoxyacetophenone

C

0.19

0.23

C

C

0.10

0.20

C

C

b 0.10 3.67 b 0.30 1.64 0.65 5.50 0.19 0.70

b 0.21 8.78 b 0.83 2.52 0.44 4.98 0.24

C

C

b 0.34

0.11 0.55 0.62

C

C

1408 1519 1576 1627 1658

0.16

0.81 b

C

C

0.12 3.78

0.15 0.81

ethyl acetate ethyl propionate methyl butanoate ethyl butanoate ethyl hexanoate (Z)-3-hexenyl acetate ethyl octanoate octyl acetate ethyl 8-hydrosybutanoate methyl benzoate ethyl benzoate y-hexalactone benzyl acetate (a-3-hexenyl heptanoate

874 955 980 1010 1241 1311 1426 1461 1484 1608 1658 1671 1695 1730

0.95 b

0.90

0.53 0.10 b b 0.10 0.65

C

C

C

C

toluene ethyl benzene o-xylene m-xylene p-xylene

1019 1098 1145 1149 1180

C

0.11 b

0.14 0.21 0.20 0.21 b

isobutyl mercaptan 2-methyl 5-propylfuran 2-pentylfuran Nfl-dimethylformamide (Z)-linalool oxide (E)-linalool oxide diethylene glycol monomethyl ether

1054 1102 1241 1304 1433 1473 1553

b b b b b b 0.16

Misc:ellaneous Compounds c diethylene glycol monoethyl ether c N-methylpyrrolidone b c diethylene glycol monobutyl ether b 0.21 dimethyl sulfone b c phenol b c diethylene glycol monophenyl ether 0.24 C

-

a Solvent

excluded. *Area percent less than 0.01.

C

C

C

b C

C

C

C

b

0.47

C

C

0.30 C

0.47 0.15

C

C

0.13 C

Ik GC peak area,’ 5% (CW2OM) white pink puree 1440 1455 1494 1559 1637 1986

b b 0.53 c c b

b b 0.42 c b 0.16

1.03 c 0.13 b c

1746 1822 1913 1922 1960

c c c b 6.32

b 0.13 2.05 c 1.13

c c c b c

1967 1968 2144

c c b

c b b

b c c

c

C

C

3,3,5-trimethyl-2-cyclohexanone

al.

Alcohols 0.11 a-terpineol c decanol c 6-mercaptohexanol 80.98 benzyl alcohol 0.75 phenylethyl alcohol c methyleugenol 0.99 3-phenylpropyl alcohol 0.13 8-nerolidol 1.85 eugenol b 7-cadinol 0.10 T-cadinol 0.10 8-cadinol c isoeugenol C a-cadinol c cinnamyl alcohol 0.10

1679 1746 1798 1822 1858 1962 1989 2003 2103 2125 2139 2150 2180 2201 2300

b b c c b c 3.14 0.56 c 0.43 0.35 0.56 0.11 0.15 b c c b 5.53 1.51 2.60 b b c b c b c c b c c b c c b b b c c c 0.10 28.31 12.27 1.71

Acids 0.10 pentanoic acid 0.19 hexanoic acid 0.48 5-hexenoic acid 0.11 octanoic acid 0.20 benzoic acid

1685 1789 1885 1998 2350

0.12 1.88 2.30 0.46 1.76

0.14 1.17 0.98 0.45 2.28

c 0.69 c 0.32 0.26

Esters 0.11 ethylphenyl acetate c phenylethyl acetate c ethylphenyl propanoate 0.13 y-octalactone c 3-phenylpropyl acetae b hexyl decanoate b (Z)-9-tetradecyl acetate b ethyl cinnamate b y-decalactone b cinnamyl acetate 0.12 &decalactone 0.21 (Z)-5-(2-pentenylpentanolide-5,1) c diethyl phthalate b y-dodecalactone

1763 1785 1846 1880 1930 2017 2049 2081 2098 2100 2153 2196 2311 2317

c b c b 7.51 b 0.37 c 0.19 2.13 0.11 0.20 b c

c c c b 6.25 c 0.25 c 0.14 1.02 0.12 0.24 c c

b c 0.87 b 0.87 c c b c 0.13 c c c b

Hydrocarbons c limonene c y-terpinene b 8-caryophyllene b a-humulene c P-bisabolene

1200 1213 1617 1685 1735

c c b c b

c c 0.20 b b

b b b c c

1589 1646 1760 1833 1933 2072

1.50 b b b b 0.45

1.95 0.26 b c c c b c b c 0.36 c

C C

Not detected.

Instruments. A Hewlett-Packard Model 5710-A gas chromatograph equipped with a flame ionization detector (FID)was used for routine gas chromatographic analysis.

Two types of wall coated open tubular (WCOT) fused silica capillary columns were used: 50 m X 0.22 mm (i.d.) coated with Carbowax 20M and 60 m X 0.22 mm (i.d.) coated with

Volatile Constituents of Guava

OV-101. The oven temperature was programmed from 80

to 200 "C at 2 OC/min. A Hewlett-Packard Model 3385-A integrator was used to determine the GC peak area. A Hitachi Model M-80 combination mass spectrometer/gas chromatograph (Hewlett-Packard Model 5710A) equipped with Hitachi Model M-6010 and 10 II/A data system was used under the following conditions: ionization voltage, 70 eV; emission current, 80 mA; ion accelerator voltage, 3100/V; ion source temperature, 200 OC. RESULTS AND DISCUSSION The volatile compounds identified in the three samples are shown in Table I along with their Kovats indices on a Carbowax 20M (IK). The major volatile constituents of guava fresh were c6 compounds in the present study. The total GC peak area percents of c6 aldehydes hexanal, (E)-3-hexenal, (2)-3hexenal, (Z)-Zhexenal,and (E)-2-hexenalwere 8% in fresh white and 34% in fresh pink. The c6 alcohols comprised 8% of the essence from both fresh white and fresh pink. The c6 acids comprised 4 % and 2% of the essence from white and pink, respectively. Therefore, the total amount of c6 compounds (aldehydes, alcohols, acids) comprised 20% of the essence of fresh white and 44% of the essence of fresh pink in the present study. Idstein and Schreier (1985) reported that 50% of the total volatiles from Brazilian guava were aldehydes. They identified hexanal and (E)-Zhexenalas major components and suggested that the presence of high amounts of (26 aldehydes and alcohols involved enzymic oxidation and reduction of c6 compounds. The presence of (26 acids agrees with this hypothesis. 3-Phenylpropyl acetate was found as one of the main components in fresh fruits, but it was detected in only a small amount in canned puree in the present study. Shiota (1978) reported 3-phenylpropylacetate as one of the minor constituents of puree from Taiwan. Idstein and Schreier (1985) identified it in the fruit pulp from Brazil and listed it in a second major group of guava constituents. Cinnamyl alcohol was the major component (28.3%) of fresh white, and it was the second major component of fresh pink (12.0%) after (E)-2-hexenal(16.9%)in the present study but it was found in only trace amount in the fruit pulp from Brazil (Idstein and Schreier, 1985). These aromatic compounds may play an important role in the characteristic sweet flavor of ripening guavas from Amami Island. It is rather surprising that canned puree contained acetoin in a large amount (81% of the total GC area of the essence). Acetoin has not been reported in guavas prior to the present study, but it was found in other tropical fruits such as mango (Hunter et al., 1974; Engel and Tressl, 1983), papaya (Yamaguchi et al., 1983), and passion fruit (Yamaguchi et al., 1983). The origin of acetoin is not understood, but it may be produced from a sugar degradation reaction (Hodge, 1967) during the canning process. Furanones, the other possible sugar degradation products, were also found in fresh fruits in relatively large quantities. 2,5-Dimethyl-4-hydroxy-3(2H)-furanone, which was reported by Idstein and Schreier (1985) for the first time as a guava fruit constituent and comprised 6.3% of the essence from fresh white. On t h e other hand, it was not detected in canned puree, suggesting a biogenetic origin. (2)-5-(2-Pentenylpentanolide-5,1), so-called jasmine lactone, is reported here as a fresh guava constituent for the first time. This &lactone was found in jasmine oil (Winter et al., 1962) and is characterized as having a peachor apricot-like note (Arctander, 1969). Wilson and Shaw (1978) reported that 0-caryophyllene was by far the largest single component among the 11

J. Agric. Food Chem., Vol. 37, No. 1, 1989

141

terpene hydrocarbons identified in guava puree from Florida. 0-Caryophyllene and limonene comprised over 95% of the hydrocarbon fraction. MacLeod and de Troconis (1982) found slightly more cy-humulene than P-caryophyllene, but neither of them was a major component of Venezuelan guava. Only trace amounts of P-caryophyllene and cy-humulene were detected in the present study. Limonene, which has been found in many varieties of guavas, was not detected in the fresh fruits and detected only trace amount in canned puree in the present study. On the other hand, bisabolene, which has also been reported in many varieties, was found in fresh fruits but not in canned puree in the present study. A unique sulfur-containing compound 6-mercaptohexanol was found in fresh fruits for the first time. It was more in fresh white (3.1%) than in fresh pink (0.6%). The difference of flavor characteristics between white and pink guavas may be partially due to the presence of this compound. 3-Pentanethiol is the only thiol compound reported in guava (idstein and Schreier, 1985) prior to the present study. Idstein and Schreier (1985) also reported many Sand N-containing heterocyclic compounds such as thiophenes, thiazoles, and pyrazines for the first time in guava. The origin of these compoundsis not clearly known because they form readily from amino acids or protein by heat treatment (Waller and Feather, 1983). Registry No. Acetaldehyde, 75-07-0; hexanal, 66-25-1; 4pentenal, 2100-17-6; (E)-3-hexenal, 69112-21-6; (q-3-hexena1, 6789-80-6;(Z)-2-hexenal, 16635-54-4;(E)-2-hexenal, 6728-26-3; furfural, 98-01-1; (E,E)-2,4-heptadiena11, 4313-03-5; benzaldehyde, 100-52-7;5-methylfurfural, 620-02-0; m-hydroxybenzaldehyde, 100-83-4;cinnamic aldehyde, 104-55-2;acetone, 67-64-1; 3-pentanone, 96-22-0; 2,3-butanedione, 431-03-8; 2,4-dimethyl-3-pentanone, 565-80-0; 2-acetylfuran, 1192-62-7; 2-propionylfuran, 35413-38-8; methyl 3194-15-8; 3,3,5-trimethyl-2-cyclohexanone, benzyl ketone, 103-79-7;5-ethyl-2(5H)-furanone, 2407-43-4; p methylacetophenone, 100-06-1; furfuryl pentyl ketone, 55107-03-4; furfuryl hexyl ketone, 87487-99-8; /3-ionone, 19-77-6; 2,5-dimethyl-4-hydroxy-3(2H)-furanone,3658-77-3; 5,6-epoxy-P-ionone, 23267-57-4; isobutyl alcohol, 78-83-1; isopentyl alcohol, 123-51-3; pentanol, 71-41-0;acetoin, 513-86-0; acetol, 116-09-6; cyclopentanol, 96-41-3;hexanol, 111-27-3;(E)-3-hexenol,928-97-2; (Z)-3-hexenol, 2,3-butanediol,513-85-9; octanol, 928-96-1; (.?3)-2-hexenol,928-95-0; 111-87-5;linalool, 78-70-6; 2-decanol, 1120-06-5;menthol, 89-78-1; furfuryl alcohol, 98-00-0; a-terpineol, 98-55-5; decanol, 112-30-1; ethyl acetate, 141-78-6;ethyl propionate, 105-37-3;methyl butanoate, 623-42-7; ethyl butanoate, 105-54-4; ethyl hexanoate, 123-66-0;(a-3-hexenylacetate, 3681-71-8; ethyl octanoate, 10632-1; octyl acetate, 112-14-1;ethyl /3-hydroxybutanoate,5405-41-4; methyl benzoate, 93-58-3; ethyl benzoate, 93-89-0; y-hexalactone, 695-06-7; benzyl acetate, 140-11-4; (Z)-3-hexenyl heptanoate, 61444-39-1;ethylphenyl acetate, 101-97-3;phenylethyl acetate, 103-45-7;y-octalactone, 104-50-7;3-phenyl propyl acetate, 12272-5; hexyl decanoate, 10448-26-7; (Z)-g-tetradecyl acetate, 16725-53-4;ethyl cinnamate, 103-36-6;y-decalactone, 706-14-9; cinnamyl acetate, 103-54-8; d-decalactone, 705-86-2; (2)-5-(2pentenylpentanolide-5,1),100428-67-9; 6-mercaptohexanol, 1633-78-9;benzyl alcohol, 100-51-6;phenylethyl alcohol, 60-12-8; methyleugenol, 93-15-2; 3-phenylpropyl alcohol, 122-97-4; @nerolidol, 108911-07-5;eugenol, 97-53-0; y-cadinol, 50895-55-1; T-cadinol, 5937-11-1; 6-cadinol, 19435-97-3;isoeugenol, 97-54-1; a-cadinol, 481-34-5; cinnamyl alcohol, 104-54-1; acetic acid, 64-19-7; isobutanoic acid, 79-31-2;butanoic acid, 107-92-6;isopentanoic acid, 503-74-2; 2-ethylbutanoic acid, 88-09-5; pentanoic acid, 109-52-4; hexanoic acid, 142-62-1; 5-hexenoic acid, 1577-22-6; octanoic acid, 124-07-2;benzoic acid, 65-85-0; diethyl phthalate, 84-66-2; y-dodecalactone, 2305-05-7; toluene, 108-88-3; ethylbenzene, 100-41-4;o-xylene, 95-47-6; m-xylene, 108-38-3;p-xylene, 106-42-3;limonene, 138-86-3;y-terpinene,99-854;/3-Caryophyllene, 87-44-5;a-humulene, 6753-98-6; 6-bisabolene, 495-61-4; isobutyl mercaptan, 513-44-0; 2-methyl-5-propylfuran, 1456-16-2; 2pentylfuran, 3777-69-3; N,N-dimethylformamide, 68-12-2; diethylene glycol monomethyl ether, 111-77-3; diethylene glycol

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J. Agric. Food Chem. 1989, 37, 142-150

monoethyl ether, 111-90-0; N-methylpyrrolidone,872-50-4; diethylene glycol monobutyl ether, 112-34-5;dimethyl sulfone, 67-71-0; phenol, 108-95-2; diethylene glycol monophenyl ether,

of guava. Phytochemistry 1982,6,1339-1342. Shiota, H. Review and study on guava fruits flavor. Koryo 1978,

104-68-7.

Stevens, K. L.; Brekke, J. E.; Stern, D. J. Volatile constituents in guava. J. Agric. Food Chem. 1970,18,598-599. Waller, G. R., Feather, M. S., Eds. The Maillard Reaction in Foods and Nutrition; ACS Symposium Series 215;American Chemical Society: Washington, DC, 1983. Wilson, G. W. 111; Shaw, P. E. Terpene hydrocarbons from Psidium guajava. Phytochemistry 1978,17,1435-1436. Winter, M.;Malet, G.; Pfeiffer, M.; Demole, E. Structure of an odorous lactone present in the essence of jasmine (Jasminum grandiflorum L.). Helu. Chim. Acta 1962, 1250. Yamaguchi,K.; Nishimura, 0.;Toda, H.; Mihara, S.; Shibamoto, T. Chemical studies on tropical fruits. In Instrumental Analysis of Foods, Recent Progress;Charalambous,G., Inglett, G., Eds.; Academic: New York, 1983;Vol. 2,pp 93-117.

LITERATURE CITED Arctander, S.Perfume and Flavor Chemicals;Published by the author: Montclair, 1969. Engel, K.-H.; Tressel, R. Studies on the volatile components of two mango varieties. J. Agric. Food Chem. 1981,31,796-801. Hodge, J. E. Symp. Foods: Chem. Physiol. Flavors, Proc. 1967, 472.

Hunter, G. L. K.; Bucek, W. A,; Radford, T. Volatile components of canned alphonso mango. J . Food Sci. 1974,39,900-903. Idstein, H.; Schreier,P. Volatile constituents from guava (Psidium guajava, L.) fruit. J. Agric. Food Chem. 1985,33, 138-143. Kunishi, A. T.;Seale, P. E. Recovery of some volatile components from mango and guava. Tech. Poro. Rep. 1961,128. MacLeod, A. J.;de Troconis, N. G. Volatile flavour components

121,23-30.

Received for review February 18,1988.Accepted June 13,1988.

Selection and Classification of Volatile Compounds of Apricot Using the RV Coefficient Pascal Schlich* and Elisabeth Guichard

The RV coefficient is a measure of similarity between two sets of variables recorded from the same sample. If the number of variables in a principal-component analysis is high, the RV coefficient allows selection of a few variables without disturbing the relative location of individuals in the first sample plots. This selection is explained and improved by a classification of variables based on the RV coefficient to define proximities between variable clusters. The groups are then submitted to a principal-coordinate analysis and minimum spanning tree using the RV matrix among the groups in order to describe relations between variable cluster. This statistical approach appears to be a very useful tool for chromatographic data handling. An example is given in a study of 56 volatile compounds quantified in 18 samples of apricots. It shows that compounds are grouped according to the chemical classes.

Progress in gas chromatography allows the separation and quantification of a great number of volatile compounds in foods or beverages. Assume that n chromatograms have been processed and p compounds quantified in each. These data are customarily arranged into a n X p matrix X,in which the ith row contains the p observations of variables (volatile compounds) recorded on the ith individual (chromatogram). The sample can be seen geometrically as a configuration of n points in a p-dimensional space. Today, principal-component analysis (PCA) (Morrisson, 1976) is a classical tool in food science, as shown in the bibliography of Martens and Harries (1983) and in the methodological paper of Piggot and Sharman (1986). PCA gives an orthogonal system of principal directions of the variance of this configuration. The answer is given by the first eigenvectors of the p x p covariance matrix C of the compounds; if the variables have been previously autoscaled, C becomes the correlation matrix. Each eigenvector is defined by a linear combination of the p compounds. Laboratoire de Recherches sur les ArGmes, Institut National de la Recherche Agronomique (INRA), 17 Rue Sully, 21034 Dijon Cedex, France. 0021-856118911437-0142$01.50/0

The interpretation of a principal component amounts to the comparison of the p coefficients of the associated linear combination. A few problems appear when p is large (for instance higher than 30). On one hand, matrix C cannot be loaded in the memory of some microcomputers and the length of computing time would be prohibitive. On the other hand, and this is the main problem, the interpretation of a linear combination of so many variables would certainly be tiresome and not very convincing, In fact, a few compounds only are generally heavily loaded on the first principal axes, while the other ones only bring a background noise. However, it is often difficult to distinguish between that noise and main information and to decide which are the relevant correlations between variables and principal components. It would be of great interest to have previous knowledge of the relevant variables and then perform the PCA with these compounds only. Moreover, if p is greater than n, PCA can be performed, but p-n dimensions of the sample configuration space are of course unnecessary. The RV coefficient (Escoufier, 1970,1973) is a measure of similarity, varying from 0 to 1, between p-dimensional and q-dimensional configurations of the same sample. It can be seen as a generalized correlation coefficient between 0 1989 American Chemical Society