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XANTHOHUMOL RESTORES HEPATIC GLUCOLIPID METABOLISM BALANCE IN TYPE 1 DIABETIC WISTAR RATS Mario Rui Lima-Fontes, Raquel Costa, Ilda Rodrigues, and Raquel Soares J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b02595 • Publication Date (Web): 26 Jul 2017 Downloaded from http://pubs.acs.org on July 27, 2017
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Journal of Agricultural and Food Chemistry
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XANTHOHUMOL RESTORES HEPATIC GLUCOLIPID METABOLISM
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BALANCE IN TYPE 1 DIABETIC WISTAR RATS
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Mário Lima-Fontes1, Raquel Costa1,2, Ilda Rodrigues1, Raquel Soares1,2,*
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Universidade do Porto, Portugal;
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Departamento de Biomedicina, Unidade de Bioquímica, Faculdade de Medicina da
I3S, Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Portugal
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*Correspondence: Raquel Soares, PhD
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Department of Biomedicine, Unit of Biochemistry
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Faculty of Medicine
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University of Porto
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Al Prof Hernâni Monteiro
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4200-319 Porto
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Portugal
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Email:
[email protected] 17
Telephone/Fax: + 351 225513624
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1 ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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Abstract
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Diabetes exhibits increased inflammation, angiogenesis and apoptosis, three processes
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attenuated by xanthohumol (XN). Herein, we evaluate the effect of XN-enriched stout
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beer consumption in hepatic glucolipid metabolism imbalance seen in type 1 diabetes
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(T1D). Five groups of Wistar rats were established: Streptozotocin-induced diabetic rats
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drinking water; treated with 5% ethanol; stout beer; stout beer supplemented with 10 mg
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XN/L; and healthy rats drinking water. Hepatic PAS, Reticulin, Sirius Red, and Oil Red
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O histological staining were performed. Lipogenic enzymes and GLUT2 expression was
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evaluated by Western blotting. Increased fibrosis in T1D animals was significantly
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decreased to control levels by XN (3.85 ± 0.38 in T1D-Beer vs 1.78 ± 0.27 in controls,
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p0.05). XN reduced
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T1D hepatic reticulin staining (9.74 ± 3.78 in T1D-Beer, p0.05). XN
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consumption interfered with T1D liver catabolic state, reversing glycogen depletion
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(22.09 ± 7.70 in T1D-Beer+XN vs 4.68 ± 4.84 in T1D-Beer, p
