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Yeast #-glucan Suppresses the Chronic Inflammation and Improves the Microenvironment in Adipose Tissues of ob/ob Mice Yan Cao, Ying Sun, Siwei Zou, Bingchao Duan, Mengying Sun, and Xiaojuan Xu J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b04921 • Publication Date (Web): 29 Dec 2017 Downloaded from http://pubs.acs.org on December 30, 2017
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Journal of Agricultural and Food Chemistry
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Yeast β-Glucan Suppresses the Chronic Inflammation and Improves the
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Microenvironment in Adipose Tissues of ob/ob Mice
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Yan Cao, Ying Sun, Siwei Zou, Bingchao Duan, Mengying Sun, and Xiaojuan Xu*
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College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072,
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China.
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*Corresponding
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[email protected] author:
Xiaojuan
Xu.
Tel/Fax:
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ABSTRACT
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Inflammation in visceral adipose tissues (VATs) contributes to the pathology of
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diabetes. This study focused on the inflammatory regulation in VATs by a yeast
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β-1,3-glucan
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pro-inflammatory modulators of TNF-α, IL-6, IL-1β, CCL2, and SAA3, and increased
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anti-inflammatory factors of Azgp1 (2.53±0.02 fold change) at protein and/or mRNA
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levels (p 0.8; b, 0.8 ≥ probability > 0.6; c, 0.6 ≥ probability > 0.4; d, 0.4 ≥ probability > 0.1.
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(B) RT-PCR confirmed the mRNA expression of the pro- and anti- inflammation
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cytokines in VATs, data represent mean ± SD of three mice per group. *p < 0.05 versus
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ob/ob mice.
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Figure 5. BYG decreased fibrosis in VATs from ob/ob mice. (A) The RNA-Seq result
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of the expression profiles of the fibrosis-related genes in VAT of ob/ob mice (two mice
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per group). a, probability > 0.8; b, 0.8 ≥ probability > 0.6; c, 0.6 ≥ probability > 0.4; d,
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0.4 ≥ probability > 0.1. (B) The representative images of masson’s trichrome stained
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VATs. (C) The statistical analysis of the masson’s trichrome staining. Data represent
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mean ± SD of three mice per group. *p < 0.05 versus ob/ob group.
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Figure 6. BYG decreased angiogenesis in VATs from ob/ob mice. (A) The RNA-Seq
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result of the expression profiles of the angiogenesis-related genes in VAT of ob/ob
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mice (two mice per group). a, probability > 0.8; b, 0.8 ≥ probability > 0.6; c, 0.6 ≥
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probability > 0.4; d, 0.4 ≥ probability > 0.1. (B) Effect of BYG on VEGF protein
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expression in VATs of ob/ob mice (four mice per group) detected by Western blotting 26
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analysis. (C) The quantitative analysis of VEGF protein expression. Data represent
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mean ± SD of three mice per group. *p < 0.05 versus ob/ob group. (D) The
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representative images of VATs stained by CD34. (E) The statistical analysis result of
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the CD34 staining. Data represent mean ± SD of three mice per group. *p < 0.05
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versus ob/ob group.
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Figure 7. BYG altered the gut microbiota and inhibited the intestine inflammation in
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ob/ob mice. Bacterial community distribution identified on the Phylum (A) and Genus
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(B) level. (C) The beta diversity heat map (bray-curtis). After treatment with BYG for
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25-30 d, feces were collected and subjected to 16s rDNA analysis. (D) Effects of BYG
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on mRNA levels of pro- and anti-inflammatory cytokines in the intestines of ob/ob
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mice. The mRNAs were extracted after BYG treatment for 30 d. Data represent mean
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± SD of three mice per group. *p < 0.05 versus ob/ob group.
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