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Zerumbone, a Cyclic Sesquiterpene from Zingiber zerumbet Induces Apoptosis, Cell Cycle Arrest and Antimigratory Effects in SW480 Colorectal Cancer Cells Thomas Sithara, B. P. Dhanya, Arun K B, Suresh Sini, Dan Mathew, Radhakrishnan Kokkuvayil Vasu, and P Nisha J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b04472 • Publication Date (Web): 25 Dec 2017 Downloaded from http://pubs.acs.org on December 26, 2017
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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Zerumbone, a Cyclic Sesquiterpene from Zingiber zerumbet Induces Apoptosis, Cell Cycle
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Arrest and Anti-migratory Effects in SW480 Colorectal Cancer Cells
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Thomas Sithara a, c, B. P. Dhanya b, c, Arun, K. B.a, Suresh Sini a, Mathew Dan d, Radhakrishnan
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Kokkuvayil Vasu b, c, *, P Nisha
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a
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Technology (CSIR-NIIST), Thiruvananthapuram, Kerala, India.
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b
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and Technology (CSIR-NIIST), Thiruvananthapuram, Kerala, India.
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c
Academy of Scientific and Innovative Research (AcSIR), New Delhi 110001, India.
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d
Plant Genetic Resource Division, Jawaharlal Nehru Tropical Botanic Garden and Research
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Institute (JNTBGRI), Palode, Thiruvananthapuram, Kerala, India.
a, c, *
Agro Processing and Technology Division, National Institute for Interdisciplinary Science and
Chemical Sciences and Technology Division, National Institute for Interdisciplinary Science
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*Corresponding Author
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Agro Processing and Technology Division,
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CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST),
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Thiruvananthapuram-695019, Kerala, India.
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E-mail:
[email protected],
[email protected],
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Tel.: +91 471 2515348; Fax: +91 471 249505
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ACS Paragon Plus Environment
Journal of Agricultural and Food Chemistry
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ABSTRACT
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Zerumbone isolated from the rhizomes of Zingiber zerumbet was for the mechanisms by which it
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exhibits antiproliferative activity in colorectal cancer cells (SW480). The results indicated that
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the zerumbone suppressed cell growth and enhanced cell apoptosis. Exposure to zerumbone
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induced generation of reactive oxygen species, reduced the cellular antioxidant status, decreased
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mitochondrial membrane potential and activated caspase 3, caspase 8 and caspase 9 (p < 0.001).
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It was also found that there was a decrease in the expression of Bcl 2 and elevation of Bax (p
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