J . Med. Chem. 1993,36, 1921-1922
1921
Additions and Corrections 1992, Volume 35
Jun Segawa, Masahiko Kitano, Kenji Kazuno, Masato Matsuoka, Ichiro Shirahase, Masakuni Ozaki, Masato Matsuda, Yoshifumi Tomii, and Masahiro K i d : Studies on Pyridonecarboxylic Acids. 1. Synthesis and Antibacterial Evaluation of 7-Substituted-6-halo-4-oxo-4H-[1,3]thiazeto[3,2-a] quinoline-3carboxylic Acids. Page 4728. In Scheme I, the reaction condition used in converting 28a-j to 29a-y was h, not g. Also, the substituents for 28a-j should be R3= Et, R7= cyclicamino, and for 29a-y, R3 = H, R7 = cyclic amino. Page 4730. In the second sentence of the third full paragraph of the second column, the order of in vitro activity against Gram-positive bacteria should read 29h > 29a > 290,29f > 29b, 29c, 29d > 29g. 1993, Volume 36
Kristin R. Snyder, Thomas F. Murray, Gary E. DeLander, and Jane V. Aldrich': Synthesis and Opioid Activity of Dynorphin A-( 1-13)NH2Analogues Containing cis- and trans-4-AminocyclohexanecarboxylicAcid. Page 1101. The first eight lines of text were repeated from the previous page, and the last nine lines of text are missing. For clarity, the sections of text involved are reproduced below. Results and Discussion Design Rationale and Synthesis. Molecular modeliig with the AMBER16J6 program was used to examine possible conformationalconstraints for incorporationinto positions 2 and 3 in Dyn A41-13)"~. These studies suggested that trans-4-aminocyclohexanecarboxylicacid (trans-ACCA) might replace Gly2-Gly3 in an extended conformation. The calculated nitrogen-carbonyl carbon distance was 5.70 A for trans-ACCA in the diequitorial conformation vs 6.12 A between the nitrogen of Gly2and the carbonyl carbon of Gly3 when the peptide was in an extended conformation. The ACCA dipeptide replacement is equivalent to constraining $2 and 43 while still allowing free rotation around 42 and $3. Tyr-Gly-Gly-Phe-Leu-Arg-Arg-Ne-Arg-R Dyn A-(I -13)NH2
Tyr-NH O C O - P h e - L e u - A r g - A r g - l l e - A r g - R [tran~-ACCA"~]DynA-(l-13)NH2 R
I
-Pro-Lya-Leu-LysNH2
ACCA was synthesized by hydrogenation of p-aminobenzoic acid with PtOz as a catalyst17(SchemeI),which yielded a mixture of cis and trans isomers (approx ratio of 2.611 cisltrans). Separation by fractional recrystalli-
zation18from EtOH yielded the cis isomer, which was pure by 'H NMR. Subsequent recrystallization from EtOHI ether yielded the trans isomer, but lH NMR indicated that the trans isomer contained from 10-15% up to 35% of the cis isomer, depending on the batch. Following fractional recrystallization, each isomer was converted separately to its Fmoc (9-fluorenylmethoxycarbonyl)derivative. Fmoc protection of these branched amino acids proved to be difficult, and literature procedureslghad to be modified20to obtain the desired product (see Experimental Section). HPLC verified that Fmoc-cis-ACCA contained only the cis isomer. In the case of Fmoc-transACCA, the contaminating cis isomer (tR = 29.3 min) was not well resolved from the desired Fmoc-trans-ACCA ( t ~ = 29;7 min) by HPLC, so this mixture was used in the synthesis of the trans-ACCA analogue of Dyn A-(l-13)"2.
Both the cis and trans isomers were incorporated separately into Dyn A4-13)"~ (Scheme I). The peptides were prepared as amides because of the enhanced metabolic stability of Dyn A-(1-13) amide vs its corresponding acid.21 The peptides were synthesizedon a PAL resin22 using Fmoc-protected amino acids by procedures described previo~sly.~~ The peptides were deprotected and cleaved from the PAL resin using trifluoroacetic acid (TFA) and purified by preparative reverse-phase HPLC. The purification of [cis-ACCAMlDynA-( 1-13)NH2 was straightforward, while the purification of the trans analogue proved difficult due to the necessity of separating
0022-2623/93/1836-1921$04.00/0 Q 1993 American Chemical Society
