(Anticodon CCC). - ACS Publications - American Chemical Society

Sequence of Human Glycine Transfer Ribonucleic Acid (Anticodon. CCC). Determination by a Newly Developed Thin-Layer Readout. Sequencing Technique ...
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Biochemistry 1980, 19, 1699-1705

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Sequence of Human Glycine Transfer Ribonucleic Acid (Anticodon CCC). Determination by a Newly Developed Thin-Layer Readout Sequencing Technique and Comparison with Other Glycine Transfer Ribonucleic Acids? Ramesh C. Gupta,* Bruce A. Roe,t and Kurt Randerath

B. A., & Randerath, K. (1979) Nucleic Acids Res. 7, The sequence of tRNA:& from human placenta 959-9701 and four other eucaryotic glycine tRNAs. Human was determined by a newly developed thin-layer readout seappears to be unusually rich in ribose-methylated quencing technique [Gupta, R. C., & Randerath, K. (1979) nucleosides. Another unusual feature of this tRNA, which Nucleic Acids Res. 6, 3443-34581. This technique enabled is shared with human but not with other eucaryotic us to display and identify about 93% of the major and the glycine tRNAs, is the presence of two methylated nucleosides modified nucleotides in the RNA chain. The remaining in its amino acid acceptor stem. The T-arm sequence of all positions were determined by methods entailing fingerprinting glycine tRNAs from higher eucaryotes has been completely of 3’-terminally 3H-labeled oligonucleotide derivatives and conserved, indicating an important as yet undetermined base-specific enzymatic cleavages. The complete nucleotide function of this particular region of these RNAs. In all animal sequence of human tRNA& is pG-C-G-C(m)-C-(m2)G-Cglycine tRNAs sequenced to date, the base between the D stem U-G-G-U-G-IC/-A-G-U-G-G-D-A-U-C-A-U-G-C-A-A-G-AU(m)-U-C-C-C-A-U-IC/m-C-U-U-G-C-G-A-C-mSC-m5C-G- and the anticodon stem appears to be capable of forming a secondary base pair with the first base of the variable arm, G-G-T-IC/-C-G-(m’) A-U-U-C-C-C-G-G-G-C-G-G-C-G-C-Aimplying the presence of a sixth Watson-Crick base pair in C-C-AoH. The sequence of human tRNA& was compared the anticodon stem of these tRNAs. with the sequences of human tRNA:%., [Gupta, R. C., Roe,

ABSTRACT:

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A b o u t 110 tRNAs have been sequenced to date from various organisms, but only 10% of these are from mammals. This has been due in part to the lack of sufficiently sensitive methods for sequence analysis of RNA, as mammalian tissues contain only small amounts of individual tRNAs (0.1-4 mg/kg of tissue). As a consequence, minor isoaccepting tRNAs from mammalian sources have not been sequenced thus far. For a compilation of sequenced tRNAs, see Gauss et al. (1979). The sequence analysis of all isoaccepting tRNAs, including the minor ones, from one particular organism represents an important step toward understanding the various interactions of tRNA with other macromolecules. Recently, several highly sensitive radioactive derivative (“postlabeling”) methods (Gupta & Randerath, 1977a,b; Donis-Keller et al., 1977; Simoncsits et al., 1977; Lockard et al., 1978; Stanley & Vassilenko, 1978; Peattie, 1979; Gupta & Randerath, 1979; Randerath et al., 1980) for sequence analysis of RNA have been developed which are applicable to the small amounts of mammalian tRNAs available. In particular, we have recently described a procedure for sequence analysis of RNA enabling one to identify the modified nucleotides and their positions in the RNA chain directly as 32P-labeledderivatives (Gupta & Randerath, 1979). In this paper, we report on the application of this method to the sequence analysis of a human glycine tRNA, tRNA:& (anticodon CCC). This tRNA was of particular interest to us for various reasons. (1) Since another human glycine tRNA t From the Department of Pharmacology, Baylor College of Medicine, Houston, Texas 77030. Received November 2, 1979. Supported by the US.Public Health Service [Grants CA-13591 and CA-I0893(P8) to K.R. and GM-21405 to B.A.R.; Research Career Development Award K04-00178 to B.A.R.] and the American Cancer Society (Grant NP-230 to B.A.R.). $Present address: Department of Chemistry, Kent State University, Kent, OH 44242.

0006-2960/80/0419-1699$01 .OO/O

(tRNA:$,) had been sequenced previously in our laboratory (Gupta et al., 1979), a comparison of the structures of the two isoacceptors was of interest. (2) The structures of glycine tRNAs from several eucaryotic sources (except mammals) are known [see Gauss et al. (1979)l; thus, it was of interest to compare the human glycine tRNAs with other known glycine tRNAs. (3) Eucaryotic glycine tRNAs are especially rich in 5-methylcytidine (Marcu et al., 1977; Garel & Keith, 1977; Ztiiiiga & Steitz, 1977; Kawakami et al., 1978; Gupta et al., 1979; Roe et al., 1976), a methylated nucleoside whose formation is strongly inhibited by the antineoplastic drug 5azacytidine (Lu et al., 1976; Lu & Randerath, 1979). Glycine tRNAs that are deficient in 5-methylcytidine may be useful in investigations on the function(s) of this methylated nucleoside (Harris & Randerath, 1978). Materials and Methods Materials. The sources of most materials used have been indicated previously (Roe, 1975; Anandaraj & Roe, 1975; Gupta & Randerath, 1979; Gupta et al., 1979). The ribosemethylated nucleoside 5’-monophosphates pAm, pCm, pUm and pGm were from P-L Biochemicals. Isolation of tRNA;&. Partially purified tRNA& was isolated from human placenta by phenol extraction at pH 4.5 in 0.14 M sodium acetate buffer, followed by adsorption on DEAE-cellulose (Roe, 1975) and several column chromatographies (Anandaraj & Roe, 1975). For subsequent further purification, column chromatography on RPC-5 was used. tRNAG’y(7.4 mg) obtained by Aminex A-28 chromatography (Anandaraj & Roe, 1975) was dissolved in 4 mL of buffer A (0.01 M Tris-HC1, pH 7.6, 0.01 M MgC12, and 0.001 M Na2S203)containing 0.3 M NaCl, applied to an RPC-5 column previously equilibrated with buffer A containing 0.3 M NaCl, and eluted with an 800-mL linear gradient of 0.3-1.2

0 1980 American Chemical Society

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GUPTA, ROE, AND RANDERATH

M NaCl in buffer A, a t a flow rate of 4 mL/5 min. After the ,4260 for alternate 4-mL fractions had been determined, aliquots (10 pL) of every fifth tube were used in a standard aminoacylation reaction to determine the elution profile [see Roe (1975) for the assay conditions]. Peak 1 (see Results) was used in the present study. The tRNA was further purified by electrophoresis a t 1000 V (constant), 12 mA, on a 20% polyacrylamide, 7 M urea, 50 m M Tris, 50 mM boric acid, and 1 m M EDTA (pH 8.3) slab gel (40 X 20 X 0.2 cm) and extracted from the gel (Chia et al., 1973). Thin-Layer Readout Technique for Sequence Analysis. This was done as described (Gupta & Randerath, 1979), with the following modifications. (1) Five micrograms of the tRNA was heated in 10 pL of water in an open tube in a boiling water bath for 45 s. (2) The gels were 0.035 cm rather than 0.06 cm thick, and 2-3-pL aliquots were applied to each well. (3) The labeled hydrolysate (Gupta & Randerath, 1979) contained 1-2 pg/pL each of the tracking dyes bromophenol blue (BP) and xylene cyano1 F F (XC). (4) Shorter fragments (chain length up to 40 nucleotides) were resolved on a 20% polyacrylamide slab gel (50 X 30 X 0.035 cm); three 2-pL aliquots of the labeled digest (Gupta & Randerath, 1979) were electrophoresed for 4.5, 9, and 14 h a t 2500 V (constant), 15 mA. (5) PEI-cellulose “prints” (Gupta & Randerath, 1979) of ladders obtained from the 20% gels were treated with RNase Tz as follows. One to two microliters of enzyme solution (0.2 unit/pL in water) was applied to a third (Gupta & Randerath, 1979) of each band of the ladder. After this had been repeated once, the treated strip was covered with Teflon tape and incubated as described (Gupta & Randerath, 1979). The enzymatically released 32P-labeled5’-terminal nucleotides were identified by contact transfer to a PEI-cellulose acceptor sheet and chromatography in 0.55 M ammonium sulfate and 1.75 M ammonium formate, pH 3.5, followed by autoradiography (Gupta & Randerath, 1979). Modified nucleotides were identified on the basis of their known chromatographic behavior (Gupta & Randerath, 1979), except for ribose-methylated residues. Since the chromatographic behavior of the latter residues, which are obtained as dinucleoside triphosphates (pNlm-N2p) by RNase T2 treatment, was not known, these compounds were degraded to nucleoside 5’-monophosphates and identified by subsequent chromatography as follows. The dinucleoside triphosphate spot(s) was cut from a chromatogram that had been developed in the ammonium formate system (Gupta & Randerath, 1979) and treated in situ with nuclease P, (0.1 pg/pL) containing 50 mM Tris-HC1, pH 7.3 (Gupta & Randerath, 1979). (Spots from ammonium sulfate chromatograms could not be used because of interference by the salt with the subsequent chromatography.) Several (up to 10) cutouts were then soaked in methanol (50 mL) for 5 min, dried, and treated in situ with a solution of snake venom phosphodiesterase (0.1 pg/pL) in 50 m M Tris-HC1, p H 8.7, and 5 m M MgC1, a t 38 OC for 2 h or overnight. The cutouts were again soaked in methanol and dried, and nonradioactive markers of pAm, pCm, pUm, and pGm (10-15 nmol each) were applied to the center of the cutout, which had been prewetted by application of 2 pL of methanol to counteract the hydrophobicity of the enzymetreated layer. The nucleotides were contact-transferred (Gupta & Randerath, 1979) to a PEIkellulose thin layer (20 cm long) and separated in acetic acid-formic acid as described (Gupta et al., 1976b), except that the chromatogram was predeveloped with methanol to the origin instead of water to avoid disturbance of the separations due to the hydrophobic nature of the donor spot. Nonradioactive compounds were located under

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100 TT(ACTI0N NUMSER

FIGURE 1: RPC-5 chromatography of 7.4 mg of partially purified tRNAG’y of human placenta (Anandaraj & Roe, 1975). After the A,, of alternate fractions had been determined, aliquots of every fifth fraction were assayed in a standard aminoacylation reaction (Roe, 1975). Peak 1 was used in the present work.

UV light, and radioactive compounds were located by autoradiography. Gel Readout Techniquefor Sequence Analysis. The tRNA ( 1 5 pg) was 5’-32P-labeled as described by Silberklang et al. (1 977), except that the R N A concentration was 1.5 pg/pL. The labeled R N A was purified on a 15% polyacrylamide denaturing gel and partially digested with alkali, RNases TI and U2 (Donis-Keller et al., 1977) and RNases A and Phy, (Gupta et al., 1979; Randerath et al., 1980). The products were resolved on sequencing gels (Donis-Keller et al., 1977; Gupta et al., 1979), and the cleavage patterns were displayed by autoradiography. Analysis of the Fragments in Complete RNase T I and RNase A Digests. Complete digestions of the tRNA (4-1 2 pg) with RNase TI or A plus alkaline phosphatase and subsequent 3H-labeling of the 3’ ends of the oligonucleotides were performed as described (Gupta et al., 1979; Randerath et al., 1980). The products were fingerprinted on PEI-cellulose thin layers, as indicated in Figure 4, and extracted from the layer (Gupta et al., 1976a, 1979). For determination of the molar ratios of individual oligonucleotides, the radioactive spots were cut from separate fingerprints derived from about 0.2 pg of t R N A and counted directly in 5 mL of scintillator (3 g of Omnifluor and 250 mL of Triton X-100 dissolved in 1 L of xylene). For determination of the nucleotide sequence each compound was subjected to base composition analysis (Gupta et al., 1976a), 3’-terminal (Randerath et al., 1974) and 5’terminal (Gupta et al., 1976a) analysis, and sequence analysis (Gupta & Randerath, 1977a,b). Results Chromatography on RPC-5 resolved the tRNAG’y isoacceptors into four species (Figure 1). Peak 1 was used in the present study. Peak 2 was shown to be tRNAg8, (Gupta et al., 1979). The minor species have not as yet been analyzed. The tRNA:& fraction obtained accepted 1.2 nmol of glycine per A260 unit. Further purification on a denaturing polyacrylamide gel showed a major band in addition to several minor faster moving bands, suggesting that the tRNA obtained from the RPC-5 column was partially nicked. In the present work, only the major band was analyzed.

HUMAN GLYCINE TRNA (ANTICODON

ccc)

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FIOURE 2: Thin-layer readout (Cupta & Randerath, 1979) of positions 5-13 of human tRNA& (A) Polyacrylamide gel patterns obtained by brief heating of the RNA in water, 5’-’*P-labelingof the fragments, gel electrophoresis, and autoradiography; ladders al: were obtained by electrophoresis for 4.5, 9, and 14 h, respectively, on a 20% polyacrylamide sequencing gel; ladder d was obtained by electrophoresis for 12 h on a 12%polyacrylamide gel. (B) Readout of the 5‘termini obtained alter transfer of fragments from the gels to PEIwllulose layers. After in situ RNase T, treatment of the fragments, the 5’-terminal S‘-’‘P-labeled nucleoside 3‘,5’-diphosphates were identified by PEI-cellulose thin-layer chromatography in 0.55 M ammonium sulfate and autoradiography. C46 and m’C47, represented by circles, were visualized after prolonged exposure (not shown) as the corresponding fragments were obtained in low yields. Broken lines indicate that two termini were found for some positions (see text). Fragment 4 (ladder d) was found to give two weak spots, C and Cm-C. after prolonged exposure (not shown).

Figure 2 exemplifies the readout of major and modified nucleotides between positions 5 and 73 of the tRNA. Chromatographic analysis of the 5’-terminal nucleotides of the labeled fragments (Figure 2A) in ammonium sulfate (Figure 2B) and ammonium formate, pH 3.5 (spot pattern not shown), gave identical results. All fragments showed distinct single termini, except for fragments 6,31, and 56, each of which gave two termini (Figure 2B) due to partial methylation at these positions as indicated by broken lines in Figure 2B. In addition, m6A was obtained at position 56 due to partial conversion of mlA to m6A under the experimental conditions. The jumps between fragments 31 and 33 and fragments 38 and 40 are due to the presence of ribose-methylated residues at positions 31 and 38, respectively (Gupta & Randerath, 1979). The 5’4erminal nucleotides of fragments 31 and 38 were identified as pUm-Up and p$m-Cp, respectively, as follows. Digestion with nuclease P, and snake venom phosphodiesterase liberated 3ZP-labeled pUm and p$m, respectively, as identified by thin-layer chromatography (Gupta et al., 1976b). The )’-terminal nucleotides were identified by sequence analysis of the RNase T I and RNase A fragments (see below). C46 and mSC47 were detectable only after prolonged exposure (not shown) because chain lengths 46 and 41 gave rather weak bands on the original gel (Figure 2A). Figure 3 exemplifies autoradiograms of two sequencing gels which enabled us to read 54 nucleotide positions of the RNA chain; this included six modified nucleotides (Cm4, mG6, $13, D19, $53, and m’A56) which were read as the corresponding major nucleotides. The data obtained were in complete agreement with those from the thin-layer readout technique (Figure 2). The remaining positions could not be identified because of the absence of enzymatic cleavage products particularly in RNase A and RNase Phy, digests (Figure 3B) and band compression (Figure 3B, OH- lane).

Parts A and B of Figure 4 depict fractionations of radioactive oligonucleotide-3‘ dialcohols obtained by complete RNase TI and RNase A digestions of tRNA& respectively, and subsequent 3’-terminal ’H-labeling of the oligonucleotides. The products in either digest were well resolved except AC‘jA-U’, G-C‘jmzG-C’, and C-U-G’j$-A-G. These wmpounds were further resolved by contact transfer and rechromatography in 0.2 N acetic acid (dinucleotides) and 0.15 M LiCl (trinucleotides). The mobilities decreased in the orders A-C‘ > m2G-C’ > G-C’ > A-U’ and C-U-G’ > $-A-G‘, respectively. The sequences of smaller oligonucleotides (chain lengths 5 3) were determined by base composition (Gupta et al., 1976a) and terminal analysis (Randerath et al., 1974; Gupta et al., 1976a). The sequences of most of the larger fragments were established by methods based on base-specific enzymic cleavage (Gupta & Randerath, 1977a,b). The positions of modified residues in the nucleotide chain were determined by base composition analysis in conjunction with 3’- and 5’-terminal analysis. $m-C’, G-Cm-C’, C(m)-C-(mz))G’, A-CmSC-mSC-G‘, A-A-G-A-Um-U’, and A-Urn-U-C-C-C-A-U$m-C-U-U-G‘ required additional information to place the modified nucleotides: base analysis (Gupta et al., 1976a) of $m-C’, G-Cm-C’, and A-A-G-A-Urn-U’ showed the absence of internal pyrimidines and no pyrimidine nucleoside trialcohol was obtained from the 3’ terminus. Because RNase Tz did not release the 3‘ terminus as a nucleoside trialcohol from any of the three compounds, the nucleotide adjacent to the 3‘ terminus was ribose-methylated. The 3’-terminal trialcohols were, however, released by nuclease PI phosphatase digestion (Gupta & Randerath, 1977a) and identified by thin-layer chromatography (Randerath et al., 1974). In an attempt to identify the ribose-methylated nucleotides by a previously described procedure (Gupta et al., 1 9 7 6 ~ involving ) 5’-32Plabeling of the alkali-resistant dinucleotides, only h-c‘ was

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Poiyacryiamiae gel reaoouts on LM poiyacryiamioe-i M urea, pti 8 . 3 , siao gcis 01 x---r-raDeieo numnn I K I Y A ~ ~Phy,’ ~ . and Phy,b are partial RNase Phy, digests at 38 and 50 “C, respectively, in the presence of 7 M urea. N denotes a position which was not enzymatically cleaved.

FIGURE 3:

4 PEkellulose map of 3‘ terminally ’H-labeled oligonucleotide dialcohols obtained by digestion with RNase T,(A) and RNase A (B) of human tRNA&, followed by chemical ’H-labeling. First dimension (LiCI gradient) was from right to left; second dimension (ammonium formate, pH 2.6. gradient) was from bottom to top. Detection was by fluorography (Randerath, 1970). CG‘, UG’, etc. are oligonucleotide-3’ dialwhol derivatives of CpG, UpG, etc. +mC‘ had migrated onto the wick attached to the top of the chromatogram shown in (B). The origins of the maps are not shown because this part of the chromatogram had been cut off prior to fractionation of the oligonucleotide derivatives (see the text). FIGURE

found to become labeled, while no labeling of Cm-C’ and Urn-U‘ was obtained under the described conditions. Because the dinucleotide dialcohols obtained by RNase T, digestion of G-Cm-C’ and A-A-G-A-Um-U‘ migrated slightly faster than markers of C-C‘ and U-U‘, respectively, on PEIFcellulose thin layers in 0.2 N acetic acid, the ribose-methylated residues appeared to be Cm and Um. Direct evidence for this was obtained by thin-layer readout sequencing of the tRNA (Figure 2). The spots labeled C(m)-C-(m’)G’ (Figure 4A) were found to be composed of mixtures of C m - C - m W and Cm-C-G‘ (faster spot) and C-C-m2G’ and C-C-G’ (slower spot) on the basis of the following evidence. Base composition analysis

(Gupta et al., 1976a) and sequence analysis (Gupta & Randerath, 1977a) showed that the faster and the slower compounds were Nm-C-(m2)G’ and C-C-(m2)G’, respectively; the ribose-methylated residue in the former was identified as C m because G-Cm-C’ was obtained by RNase A digestion and ’H-labeling (Figure 4B). Cm-C-(m2)G’ contained 46% m’G, as determined by base composition analysis, while C-C-(m2)G’ contained 26% m2G. The content of Cm was 75% as deduced from the molar ratio of the G-Cm-C‘ spot (Figure 4B). On this basis, the total content of m2G in C(m)-C-(m2)G was calculated to be 41%. In A-C-mSC-mSC-G’,the two mSC residues were placed as shown because G-A-C’ but not G-A-m’C’ was present in the RNase A digest (Figure 48). In A-Um-U-C-C-C-A-U-~-C-U-U-G’,the ribose-methylated nucleotides at positions 2 and 9 were identified as Um and $m, respectively, because A-A-G-A-Um-U’ and $m-C’ were obtained by RNase A digestion of the R N A and ’Hlabeling (Figure 4B). The minor products A-U-U-C-C-C-G‘ (Figure 4A), G-A-U‘ (Figure 4B), and A-A-G-A-U‘ (Figure 48) all were present at a molar ratio of about 0.06, indicating slight undermethylation of m’A and Um. The fragments G-Cm-C (Figure 4B) and C(m)-C-(m’)G (Figure 4A) were connected to extend the sequence from position 5 (Figure 2) to position 3 to deduce the complete sequence of the tRNA. The 3’ terminus of the R N A was obtained by 3’-terminal analysis of C-A-C-C-A’ (Figure 4A). Positions 1 and 2 were determined as G and C, respectively, by 5’-32P-labeling of the tRNA, followed by base-specific enzymatic digestions and thin-layer chromatography of the products as previously described (Gupta & Randeratb, 1977a.b). On the basis of these results, an unambiguous sequence of human tRNA$$= was deduced, as shown in Figure 5.

HUMAN GLYCINE TRNA (ANTICODON

ccc)

FIGURE 5 : Primary structure of human tRNA:& in cloverleaf form. The enclosed regions indicate nucleotides that are common to both human tRNAg& and t R N A & (Gupta et al., 1979).

Discussion Since human tRNA:$- is the first tRNA whose sequence has been determined by the newly developed thin-layer readout technique (Gupta & Randerath, 1979), we shall briefly comment on this technique and then compare the structural features of human tRNA$- with the structures of other eucaryotic glycine tRNAs. The thin-layer readout technique for sequencing of RNA was developed for two main reasons. (1) Recently developed readout sequencing techniques based on base-specific cleavages (Gupta & Randerath, 1977a,b; Donis-Keller et al., 1977; Simoncsits et al., 1977; Lockard et al., 1978; Peattie, 1979; Randerath et al., 1980) do not enable one to locate and identify modified nucleotides in the RNA chain. Because most RNAs contain modified nucleotides, this must be regarded as a major deficiency of the gel readout procedures for sequencing RNA. The thin-layer readout procedure provides a means of identifying modified nucleotides directly as 32P-labeledderivatives. (2) Highly base-paired regions are resistant to controlled enzymatic hydrolysis, which makes it impossible to read the sequence in some regions of the RNA because the corresponding bands are missing in the gel. However, cleavage of phosphodiester bonds in base-paired regions occurs more readily under conditions of partial chemical hydrolysis (Stanley & Vassilenko, 1978; Gupta & Randerath, 1979; Peattie, 1979) of the RNA. As shown in Figure 2, the thin-layer readout technique enabled us to display 93% of the sequence of human tRNA&. On the basis of the gel readout technique, on the other hand, we were able to identify correctly only 48 of the 74 nucleotides of the RNA (Figure 3), Le., about 65% of the sequence. The remaining nucleotides either were misidentified (because of the presence of posttranscriptional modifications) or failed to give bands on the gel (because of tight base pairing). Insufficient enzymatic cleavage was particularly apparent in the T arm and adjacent regions (nucleotides 46-65) (see Figure 3B), a region of tRNA known to be involved in tight secondary and tertiary base-pairing interactions [see Rich & RajBhandary (1976)l. Those nucleotides that could not be identified by the readout techniques were analyzed by a simple 3H fingerprinting technique which has been reviewed recently (Randerath et al., 1980) and therefore will not be discussed here. For example, additional evidence for C46 and mSC47, which, for as yet

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undetermined reasons, gave weak bands on the thin-layer readout film (Figure 2A) and could not be visualized at all on the gel readout films (Figure 3B), was provided by 3’terminal analysis of the RNase A fragment G-A-C’ (Figure 4B) and by base composition analysis of the RNase T I fragment A-C-mSC-mSC-G’(Figure 4A). The slight modifications (see Materials and Methods) of the published procedure (Gupta & Randerath, 1979) were found useful in the sequence analysis of human tRNA&; they may be applicable to other RNAs also. Thus, heating the RNA in water at 100 OC for 45 s afforded a more uniform cleavage of the phosphodiester bonds than did heating at 80 OC for 6 min, and thinner (0.035-cm) gels gave sharper bands than did 0.06-cm thick gels. The use of 20% polyacrylamide gels to resolve the shorter fragments resulted in greater distances between adjacent bands. This was found particularly important in the analysis of 3’4erminal fragments which may be somewhat contaminated on 12% gels with overdigestion products or other impurities derived from traces of contaminating RNAs. The treatment of individual bands of the ladder with RNase TZ,in addition, enabled us to obtain single termini from the shorter fragments (Figure 2B). The sequences of many eucaryotic glycine tRNAs, Le., human tRNA:$- (Gupta et al., 1979), silkworm tRNA:$(Garel & Keith, 1977; Zcfiiga & Steitz, 1977), wheat germ tRNA& (Marcu et al., 1977), yeast tRNA$c (Yoshida, 1973), silkworm tRNA:!$-’ (Kawakami et al., 1978), and human tRNA$- (present work), are now known. Thus, more is known about the sequences of eucaryotic glycine tRNAs than about the sequences of any other family of eucaryotic tRNA isoacceptors; cf. the compilation of tRNA sequences by Gauss et a]. (1979). A comparison of the various eucaryotic glycine tRNA isoacceptors appeared of interest therefore. We shall first compare the structure of human tRNA:& with that of human tRNAg$- (Gupta et al., 1979) and then discuss the noteworthy structural features of all eucaryotic glycine tRNAs. In Figure 5, the enclosed regions indicate nucleotides common to both human glycine tRNAs. If differences in posttranscriptional modifications are excluded, the two RNAs exhibit a homology of 67.6%. Like all other eucaryotic glycine tRNAs, human tRNA& has a ribose-methylated pyrimidine nucleoside in position 4. Two additional ribose-methylated nucleosides in the anticodon arm are found only in tRNA&. In contrast to other eucaryotic glycine tRNAs, the human RNAs contain m% in position 6. While m2G has been found mostly in position 10 of tRNAs, its occurrence in position 6 does not appear to be rare in mammalian tRNAs, since, in addition to the mammalian glycine tRNAs, mammalian methionine (Petrissant & Boisnard, 1974; Piper, 1975) and leucine (E. Randerath, R. C . Gupta, R. J. Rhines, and K. Randerath, unpublished experiments) tRNAs have m% in position 6. The human glycine tRNAs are the only tRNAs known to have two methylated nucleosides in the amino acid acceptor stem. They are rich in m5C, as has been found also for glycine tRNAs from other higher eucaryotes (Garel & Keith, 1977; Zcfiiga & Steitz, 1977; Marcu et al., 1977; Gupta et al., 1979). The reasons for the resistance to methylation of C46 in tRNA& are unknown; this position is completely methylated to mSC in human tRNAg$, (Gupta et al., 1979). In Figure 6, sequences of all known eucaryotic glycine tRNAs have been compared. The following points appear noteworthy.



U* denotes a mixture of two unknown modified uridines (Kawakami et al., 1978).

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6 : Comparison of eucaryotic tRNAG'y sequences. The sequence of human tRNA& (Gupta et al., 1979) is presented at the top. In the other t R N A , only those nucleotides are shown which are different from the corresponding nucleotides in human tRNA&. The underlined nucleotides indicate differences in posttranscriptional modifications only. Position 43 in yeast tRNA& is absent. Silkworm tRNA{p& contains an additional C residue in the D loop. FIGURE

(1) The loop sequences have been largely conserved. Only the 3'-terminal position of the anticodon loop exhibits a high degree of variability. Conservation of loop sequences has been observed also in eucaryotic cytoplasmic initiator tRNAs and eucaryotic phenylalanine tRNAs but not in other tRNA isoacceptors [see Gauss et al. (1979)l. (2) The T arm (nucleotides 47-63) appears to constitute the longest conserved sequence in the eucaryotic glycine tRNAs; only yeast tRNA& shows a G