Correction-Mutations in Escherichia coli Altering an Apurinic

D. Kirtikar, G. Cathcart, J. White, I. Ukstins, and D. Goldthwait. Biochemistry , 1978, 17 (21), pp 4578–4578. DOI: 10.1021/bi00614a600. Publication...
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CORRECTION Mutations in Escherichia coli Altering an Apurinic Endonuclease, Endonuclease 11, and Exonuclease 111, and Their Effect on in Vivo Sensitivity to Methylmethanesulfonate, by D. M. Kirtikar, G. R. Cathcart, J. G . White, I. Ukstins, and D. A. Goldthwait,* Volume 16, Number 25, December 13, 1977, pages 5625-563 1 . We have been unable to repeat the isolation of an enzyme fraction from Escherichia coli previously reported (as noted above as well as by Kirtikar, D. M., Cathcart, G. R., & Goldthwait, D. A. (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 4324) to be active against D N A treated with methylmethanesulfonate, methylnitrosourea, 7-bromomethylbenz[a]anthracene, and y irradiation. This activity, designated endonuclease 11, was reported to be eluted from DEAE-cellulose with 0.25 M sodium chloride. In over 35 different experiments under a variety ot'conditions, the DEAE column elution patterns have not shown this activity. Because of this extensive work and because of other experiments which were designed to verify various aspects, we feel a t the present time that we are unable to substantiate the claims made for endonuclease I1 in the publications noted.