Table II. Analyses of Two Monosaccharide Mixtures
Recovery, yoComponents A" Bb D-Fucose 98.2 98.0 &Arabinose 100.8 99.2 n-Ribose 100.8 99.0 n-Mannose 98.9 98.2 D-Galactose 98.9 98.0 D-Glucose 102.4 97.8 0 5.0 mg. of each component. * 2.5 mg. of each component.
acetylated derivatives. The resulting mixtures of acetylated derivatives were separated and quantitated with xylitol as the internal standard (Table 11). Data obtained indicate that both reduction and acetylation of monosac-
charides are quant,itative and that the method is excellent for analysis of glycose mixtures. LITERATURE CITED
(1) Abdel-Akher, M.,Hamilton, J. K., Smith, F., J . Am. Chem. SOC.73, 4691
(1951). (2) Bishop, C. T., Can. J . Chem. 38, 1636 (1960). (3) Bishop, C. T., Advun. Carbohydrate Chem., 19, 95 (1964). (4)Bishop, C. T., Cooper, F. P., Murray, R. K., Can. J . Chem. 41,2245 (1963). (5) Gunner, S. W., Jones, J. K. N., Perrv. M. B.. Chem. Ind. (London) 196l"255. (6) Gunner, S. W., Jones, J. K. N., Perry, M. B., Can. J . Chem. 39, 1892 (1961). (7) Hause, J. A,, Hubicki, J. A., Hazen, G. G., ANAL.CHEM.34, 1567 (1962). (8) Richey, J. hl., Richey, M. G., Jr., Schraer. R.. Anal. Biochem. 9.272 (1964). (9) Sawardeker, J. S., Sloneker, J. H:, ANAL. CHEM.37, 945 (1965).
(10) Sweeley, C. C., Bentley, R., Makita, M., Wells, D. D., J . Am. Chem. SOC. 85, 2497 (1963). (11) Sweeley, C. C., Walker, B., ANAL. CHEM.36, 1461 (1964). (12) VandenHeuvel, W. J. A., Homing, E. C., Biochem. Biophys. Res. Commun. 4,399 (1961). JAWAHAR S. SAWARDEKER JAMES H. SLONEKER ALLENEJEANES
Northern Regional Research Laboratory Peoria, Ill. Division of Carbohydrate Chemistry, 150th Meeting ACS, Atlantic City, N. J., September 1965. The Northern Regional Research Laboratory is a laboratory of the Northern Utilization Research and Development Division, Agricultural Research Service, U. s. Department of Agriculture. hlention of firm names or trade products does not imply that they are endorsed or recommended by the Department of Agriculture over others not mentioned.
Determination of Magnesium in Uranium by Atomic Absorption S pectro metry SIR: Magnesium may be determined in luranium by atomic absorption spectrometry (AAS), using the method of additions, without prior separation. Previously this method has been used only after separation of most of the uranium by solvent extraction (1). The procedure consists simply of bringing the sample into a hydrochloric acid solution, dividing into three or more aliquots to which are added different quantities of a magnesium standard solution, and measuring the atomic absorbances. If hydrogen peroxide is required to dissolve the sample, it is subsequently destroyed by evaporating to dryness and redissolving in acid. The standard solution is magnesium metal dissolved in a minimum volume of hydrochloric acid and diluted as required. Atomic absorption measurements were made by the author at
Table I.
Results with Several Samples
Range of Mg
By AAS, Sample p.p.m. 1-A 6.5 1-B
2-A
2-B
3 4 5
1604
6.S
8.4 8.3 7.6 3.6 0.6
std. additions, pg.
By.
emismon spectrography, p.p.m.
0-30
... ...
0-60
10 f ca. 3
0-30 C-30 e30 0-60 0-75
