Development of a Novel Process Integrating the Treatment of Sludge

Aug 13, 2015 - Department of Mechanical, Aerospace and Civil Engineering, Brunel University, Kingston Lane, UB8 3PH Uxbridge, Middlesex,. U.K.. §...
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Development of a novel process integrating the treatment of sludge reject water and the production of polyhydroxyalkanoates (PHAs) Nicola Frison, Evina Katsou, Simos Malamis, Adrian Oehmen, and Francesco Fatone Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.5b01776 • Publication Date (Web): 13 Aug 2015 Downloaded from http://pubs.acs.org on August 24, 2015

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Title: Development of a novel process integrating

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the treatment of sludge reject water and the

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production of polyhydroxyalkanoates (PHAs)

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Nicola Frison,† Evina Katsou,†,‡ Simos Malamis,†,§ Adrian Oehmen,¥ Francesco Fatone*,†

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†University of Verona, Department of Biotecnology, Strada Le Grazie 15, 37134 – Verona,

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Italy

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Department of Mechanical, Aerospace and Civil Engineering, Brunel University, Kingston

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Lane, UB8 3PH Uxbridge, Middlesex, UK.

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§

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Engineering, National Technical University of Athens, 5 Iroon Polytechniou St., 15780,

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Athens, Greece.

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¥

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Universidade Nova de Lisboa, 2829-516 Caparica, Portugal.

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*Corresponding author: tel/fax: +39 045 8027965, e-mail: [email protected].

Department of Water Resources and Environmental Engineering, School of Civil

UCIBIO, REQUIMTE, Departamento de Química, Faculdade de Ciências e Tecnologia,

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ABSTRACT. Polyhydroxyalkanoates (PHAs) from activated sludge and renewable organic

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material can become an alternative product to traditional plastics since they are biodegradable

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and are produced from renewable sources. In this work, the selection of PHA storing bacteria

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was integrated with the side stream treatment of nitrogen removal via nitrite from sewage

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sludge reject water. A novel process was developed and applied where the alternation of

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aerobic-feast and anoxic-famine conditions accomplished the selection of PHA storing

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biomass and nitrogen removal via nitrite. Two configurations were examined: in

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configuration 1 the ammonium conversion to nitrite occurred in the same reactor in which the

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PHA selection process occurred, while in configuration 2 two separate reactors were used.

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The results showed that the selection of PHA storing biomass was successful in both

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configurations, while the nitrogen removal efficiency was much higher (almost 90%) in

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configuration 2. The PHA selection degree was evaluated by the volatile fatty acid (VFA)

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uptake rate (-qVFAs) and the PHA production rate, which were 239±74 and 89±7 mgCOD

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per gram of active biomass (Xa) per hour, respectively. The characterization of biopolymers

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after the accumulation step, showed that it was composed of 3-hydroxybutyrate (3HB) (60%)

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and 3-hydroxyvalerate (3HV) (40%). The properties associated with the produced PHA

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suggest that they are suitable for thermoplastic processing.

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Keywords: polyhydroxyalkanoate (PHA); feast and famine regime; nitrogen removal

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via nitrite; sludge reject water

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INTRODUCTION

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Polyhydroxyalkanoates (PHA) are biodegradable polymers that can be produced by many

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different types of bacteria. Compared to conventional synthetic polymers, PHAs possess

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obvious ecological advantages since they are completely biodegradable and nontoxic1,2 and

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can be produced from a renewable source. PHAs, which encompass polyhydroxybutyrate

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(PHB) homopolymers, polyhydroxybutyrate-co-hydroxyvalerate copolymers (PHB/V) and

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other related linear polyesters, have significant opportunities for marketability. PHA

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production from mixed cultures and renewable organic wastes as a carbon source

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has

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become very attractive over the last years due to the decrease in the production cost of the

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process6,7. Activated sludge from wastewater treatment plants (WWTPs) is a well-known

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source of PHA-storing organisms that store these polymers as carbon and energy reserve for

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biomass growth. PHA production from mixed cultures are accomplished by a sequence of

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operations which are the following8,9: i) acidogenic fermentation to produce volatile fatty

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acids (VFAs) from biodegradable organics; ii) selection of PHA storing biomass in a

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sequencing batch reactor (SBR); iii) a batch process to maximise the PHA accumulation in

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the bacteria.

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The carbon excess and limitation strategy under feast and famine conditions has been found

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to be favourable for the enrichment and long-term cultivation of PHA producing

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communities. Nitrogen limitation is a successful strategy that can be employed to accomplish

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high PHA contents during the PHA production step 10. However, these processes are aerobic

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and thus are energy intensive; it is estimated that approximately 39 MJ are needed to produce

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1 kg of PHA when the aerobic accumulation step is employed11,12.

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The integration of nutrient removal in wastewater treatment systems with the PHA

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production cycle is currently a challenge. Jiang et al. (2009)13, Morgan-Sagastume et al.

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(2010)14 and Pittmann et al. (2014)15 evaluated the potential for the enrichment of PHA-

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storing organisms and the PHA-storage capacity of fermented sludge under different

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operational conditions. It was found that PHA producing organisms could be successfully

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enriched using the fermented sludge as feedstock, although very high nutrient loads did limit

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the maximum level of PHA accumulation14. Morgan-Sagastume et al. (2010)14 also examined

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a new concept of WWTP operation, where the selection of PHA storing biomass was

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accomplished based on the conversion of the readily biodegradable chemical oxygen demand

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(COD) from municipal wastewater without its pre-conversion to VFAs16. However, post-

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treatment was required, in particular for enhanced nutrient removal of the treated effluent.

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Anterrieu et al (2014)17 studied the integration of PHA production with conventional

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nitrification and denitrification for the treatment of sugar beet factory waters, by operating an

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anoxic feast and aerobic famine phase. In the sludge treatment line of municipal wastewater

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treatment plants, however, the nutrient loads are typically much higher, and side-stream

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treatment is an attractive option to avoid recycling these nutrients to the main treatment line.

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Short-cut nitrogen removal processes via the nitrite pathway have been found to provide an

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attractive means of achieving nutrient removal from sludge reject waters at lower operating

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expenses compared to conventional nitrification-denitrification process18. Frison et al.

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(2014)19 compared the potential PHA storage of sludge under nitrifying and non-nitrifying

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conditions in batch reactors, and found PHA production yields of 0.6 Cmol PHA/Cmol VFA

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in both systems. Nevertheless, the feasibility of integrating PHA production with nutrient

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removal via nitrite has not been investigated.

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In this work, a novel process treating sludge reject water that integrates the side-stream

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biological nitrogen removal via nitrite process with the selection of mixed culture PHA

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storing biomass is examined in large lab-scale reactors fed with real wastewater. A modified

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pathway for nitrogen removal was developed and applied in order to induce a feast and

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famine regime under aerobic and anoxic conditions, respectively. The novel approach is

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based on (i) the selection of PHA storing biomass in a sequencing batch reactor (SBR) by the

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alternation of aerobic feast conditions for ammonia conversion to nitrite followed by anoxic

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famine conditions for denitritation driven by internally stored PHA as carbon source; (ii)

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establishment of the desired COD/N ratio for the selection of PHA storing biomass by

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controlling the dose of the external carbon source and the nitrogen contained in the sludge

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reject water. A side-stream treatment technology able to integrate the conversion of

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fermented sludge to PHAs with nutrient removal via nitrite would enable the simultaneous

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reduction of nutrient loads to the main line of the WWTPs, lowering operational costs, with

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the recovery of a valuable resource, PHA, providing further economic advantages to the

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WWTP.

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MATERIAL AND METHODS

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2.1 Experimental set-up

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Figure 1 shows two alternative configurations applied for nitrogen removal via nitrite and the

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selection of PHA storing biomass downstream from the anaerobic digester. In configuration 1,

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the nitrogen removal through nitritation/denitritation and the PHA selection process occurred

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in a single SBR by applying a feast and famine regime. Specifically, during aerobic

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conditions, ammonium was oxidized to nitrite and the VFAs converted to PHA, while during

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the anoxic famine conditions, nitrite was reduced to nitrogen gas (N2) using the internally

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stored PHAs as carbon source (Figure 1a). Ideally, the aerobic reaction should coincide with

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the feast phase and the anoxic reaction with the famine conditions. Good selection of PHA

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storing biomass has been observed with the decrease of the feast/famine ratio24. However, in

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this work, the aerobic reaction time should also be long enough to ensure adequate

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availability of nitrite for denitritation. The external carbon source was only added during the

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beginning of the aerobic phase. Consequently, an efficient famine condition under anoxic

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conditions can occur when enough nitrite is available for the complete utilization of PHA, in

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the absence of biodegradable organic matter. The theoretical stoichiometry of the process

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imposes that the ratio of PHA stored (as COD) to the nitrite denitrified (i.e. gCODPHA/gNO2-

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N), should not exceed the stoichiometric value of 1.72/(1-YHD). The heterotrophic yield

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under anoxic conditions (YHD) is equal to 0.54 gCODproduced/gCODremoved27,31. In

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configuration 2, the ammonium conversion to nitrite and the selection of the PHA storing

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biomass (Figure 1b) were accomplished in two separate SBRs. Nitritation was carried out in a

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dedicated SBR and then the liquid was fed to the selection reactor while operated under

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famine conditions, accomplishing the denitritation using the stored PHA. In the selection

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reactor, the COD/N ratio was adjusted by feeding the external carbon source based on the

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sludge reject water fed, which was the main source of nutrients (nitrogen and phosphorus).

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The PHA accumulation step of the examined process was performed in a fed-batch reactor to

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maximize the cellular PHA content of the biomass harvested from the selection stage. The

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volatile fatty acids (VFAs) that are required for the feast conditions in the selection reactor

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and the PHA accumulation in the batch reactor were recovered from the acidogenic

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fermentation of sewage sludge. Fermented liquid from sewage sludge (SFL) was used as

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VFA source. The process is described in detail in the work of Longo et al. (2015)20. The

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technical details of the applied configurations are given below. In total the SBR was operated

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for 150 days, where sludge inoculum was added only at day 1 of operation.

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2.1.1 Configuration 1 (Figure 1a). This configuration combined the PHA biomass selection

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and nitrogen removal via nitrite process in only one SBR. The SBR had a working volume of

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26 L. Full details of the SBR are reported in the supporting information (SI). The cycles of

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the SBR consisted of 5 min of feeding, 300 min of reaction phase (aerobic and anoxic), 15

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min of settling and 5 min of discharge. As a first starting cycle, the length of the aerobic

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phase was set up at the same length of the feast phase (50 min). Then, the length of the

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anoxic phase (250 minutes) was derived by imposing a feast/famine ratio of 0.2, which is

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recommended in order to achieve good selection of PHA storing biomass24.

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The impact of combining the ammonium oxidation to nitrite, denitritation and selection of the

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PHA storing biomass in a single reactor was evaluated by altering the percentage of the

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aerobic versus the total cycle duration. The aerobic reaction duration varied from 17 to 30%

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(Period 1, days 0-50) and from 30 to 50% (Period 2, days 51-108) of the total cycle duration.

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This variation was introduced in order to compare the effect of the availability of nitrite on

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the process. Two experimental periods were performed using configuration 1 and adopting

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different COD/N ratios (Table 1).

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The target COD/N was achieved by supplying the same volume of sewage sludge

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fermentation liquid (SFL) according to the organic loading rate of VFA (OLRVFA) of 623±55

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(Period 1) and 684±43 gCODVFA/m3d (Period 2) and altering the volumetric nitrogen loading

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rate (vNLR) by adjusting the feeding of the sludge reject water. The total organic loading rate

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(total vOLR) applied to the system was 716±32 and 820±62 gCOD/m3d, respectively, for

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Period 1 and Period 2, where the sludge reject water accounted for less than 25% of the total

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vOLR applied. Table 1 reports the operating conditions adopted during the examined periods.

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In the first period, the feast conditions were established at the beginning of the aerobic phase

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by applying a COD/N ratio of 5.6±0.1 gCODVFA/gNH4-N, which was similar with others

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using sewage sludge fermentation liquid as carbon source14,15. In the second period, the initial

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COD/N was decreased to 2.1±0.02 gCODVFA/gNH4-N by the increase of the volumetric

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nitrogen loading rate (vNLR) up to 380±29 gN/m3d. The solids retention time (SRT) was

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kept within the range of 12-15 days throughout each experimental period. During period 1,

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transient conditions prevailed as the process was not sustainable due to the very high

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CODPHA/NO2-N at the beginning of the anoxic phase. To overcome this problem the length

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of the aerobic phase was increased in order to promote nitritation, and some PHA

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consumption. In Period 2, the length of the aerobic phase was maintained at 135±16 min,

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which corresponded to 45±12% of the total cycle duration. The small variation in the length

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of the aerobic period was introduced to balance the variability of the nitrification rate due to

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temperature variations and thus have a similar nitrite concentration at the end of the aerobic

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phase. In this way, the CODPHA/NO2-N ratio was maintained relatively constant when the

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anoxic phase started during period 2, and was considered to be a key factor for defining the

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SBR operational cycle.

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(Table 1)

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(Figure 1a and 1b)

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2.1.2 Configuration 2 (Figure 1b). This Configuration involved two SBRs, one nitritation

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SBR (N-SBR) dedicated solely to the nitritation process and another SBR for the selection of

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PHA storing biomass and the denitritation process. The N-SBR was applied as a pre-

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treatment stage to enhance the oxidation of ammonium to nitrite (nitritation). The N-SBR

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was operated in the following sequence: 15 min feeding, 4 h aeration reaction phase, 30 min

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settling, 15 min decanting. A detailed description of the operating conditions of the N-SBR is

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given in the SI. The nitrified sludge reject water exiting from the N-SBR reactor contained

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ammonium in the range of 25 to 67 mgN/L, while the average level of nitrite was

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approximately 350 mgN/L. After settling, the clarified effluent from the N-SBR was

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temporarily collected in a storage tank (80 L of volume) and was then fed to the selection

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SBR during the first 10-12 minutes of the anoxic phase based on a volumetric nitrogen

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loading rate of 530±11 gN/m3d, which corresponded to a volumetric nitrite loading rate of

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423±95 gNO2-N/m3d. The loading rate of the carbon source during the feast was balanced

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with the nitrite loading rate applied during the famine phase based on the ratio 2.2±0.1

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gCOD/gNO2-N. The operational cycle of the selection SBR consisted of 50 minutes of

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aerobic phase, followed by 250 minutes of anoxic conditions. The length for the feeding,

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settling and discharging phases were respectively 15, 30 and 15 minutes. The SRT was kept

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in the same range as for Configuration 1, 12-15 days.

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2.2

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The biomass was collected under famine conditions (end of the anoxic phase from the

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selection SBR) and was concentrated gravimetrically by applying 30 min of settling. The aim

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was to reduce the level of nutrients contained in the biomass obtained from the selection SBR.

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Then, the biomass was placed in triplicate fed-batch glass reactors with working volumes of

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1 L that were equipped with blowers, diffusers and probes for the measurement of the

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dissolved oxygen (DO, type WTW, CellOx® 325), the pH (Polyplast Pro) and the

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temperature (PT100). The on-line signals were automatically recorded. The DO level was

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always maintained above 2 mg/L. The oxygen uptake rate (OUR) was determined using the

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respirometer MARTINA (SPESS, Italy). The substrate was divided in fixed volume aliquots

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in order to dose manually each time approximately 1 gCOD/L of VFAs. New aliquots were

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dosed when consecutive OUR values were ~ 50% less than the previously recorded values. In

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order to test different COD/N/P ratios during the PHA accumulation, three different carbon

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sources were applied; a synthetic mixture of VFA (COD/N/P 100:0:0), sewage sludge

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fermentation liquid (SFL) (COD/N/P 100:9.7:2.1) and sewage sludge fermentation liquid

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with wollastonite (WSFL) (COD/N/P 100:7.8:0.06). In the latter, the use of wollastonite

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during fermentation improves the CODVFA/NH4-N/PO4-N ratio, since it limits the net release

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of nutrients (ammonium and phosphate). The duration of the accumulation test was 6-8 h.

PHA accumulation

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2.3

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In this study the VFAs concentration, expressed as mgCOD/L, was calculated as follows:

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Calculations

VFAs=∑ (HAc + HPr + HBt+ iso-HBt + HPt + isoHPt + HHe + HHp)

(1)

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where: HAc is acetic, HPr is propionic, HBt is butyric, iso-HBt is iso-butyric, HPt is

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pentanoic, isoHPt is iso-pentanoic, HHe is hexanoic and HHp is heptaoic acid.

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The PHA concentration was converted into COD units following the oxidation stoichiometry:

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1.67 mgCOD/mgPHB, 1.92 mgCOD/mgPHV and 2.10 mgCOD/mgPHH. The total PHA

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concentration was calculated as sum of PHB, PHV and PHH concentrations:

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PHA (mgCOD/L) =∑(PHB+PHV+PHH)

(2)

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The amount of PHA (g/L) was subtracted from the VSS (g/L) to calculate the concentration

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of non-polymer biomass, or active biomass (Xa, g/L). The latter was transformed as COD

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concentration by the stoichiometric ratio of 1.42 gCOD/gVSS26.The fraction of the PHA in

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the biomass was calculated considering the following equation: PHA(%) =

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୥୔ୌ୅ ୥୚ୗୗ

× 100

(3)

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where the gPHA and the VSS were respectively the PHA and the volatile suspended solids of

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the biomass. The specific VFA uptake rate (-qVFA, mgCOD/gXah) and the PHA storage rate

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(qPHA, mgCOD/gXah), were determined by linear regression analysis by plotting the

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concentration of VFAs and PHA as a function of time. The results were normalized for the

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active biomass concentration Xa and reported at the same operating temperature of the

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reactor,

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gCODPHA/gCODVFA) and the growth yield (YX/VFA, gCODX/gCODVFA) were calculated as the

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ratio between each maximum specific rate (qPHA and qX, respectively) and the -qVFA.

which

varied

between

21-27

°C.

The

PHA

storage

yield

(YPHA/VFA,

239 240

2.4

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The concentrations of the mixed liquor suspended solids (MLSS), mixed liquor volatile

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suspended solids (MLVSS), chemical oxygen demand (COD), soluble chemical oxygen

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demand (sCOD), total Kjeldahl nitrogen (TKN), ammonium (NH4-N), and total phosphorous

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(TP) were determined according to standard methods22. Nitrite (NO2-N), nitrate (NO3-N) and

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phosphate (PO4-P) concentrations were determined by the ion chromatograph Dionex ICS-

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900 with AS14 as column, while the concentrations of HAc, HPr, HBt, iso-HBt, HPt, isoHPt

Analytical methods

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and HHe and heptanoic acid were determined by liquid chromatography through a Dionex

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ICS-1100 with IonPac ICE-AS1 as column. Samples of biomass were collected from the

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selection and accumulation reactors and the liquor was removed through centrifugation. Then,

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the thickened biomass was freeze-dried with a lyophilisation unit (Lio 5P, 5Pascal, Milano,

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Italy) and analysed for the content of PHA using the method developed by Lanham et al.,

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(2013)23. The specific ammonium uptake rate (sAUR) and specific nitrogen uptake rate

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(sNUR) were determined in situ following the procedures that are given in SI. The sAUR and

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sNUR were corrected to the reference temperature of 20oC using the Arrhenius equation. The

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nitrogen mass balances were calculated for each experimental period (see SI).

256 257

The final biopolymers produced during the accumulation stage were also extracted from the

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freeze-dried biomass by applying the chloroform method (50 mL/g of freeze-dried biomass)

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followed by the addition of methanol as precipitation agent1. Then, the biopolymers were

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analysed through size exclusion chromatography (SEC, Polymer Lab) in order to determine

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the molecular number (Mn), average molecular weights (Mw) and the polydispersity indices

262

(PDI). The glass transition temperature (Tg), melting temperature (Tm) and melting enthalpy

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(DHm) were determined by differential scanning calorimetry (DSC, TA Instruments). More

264

information about the procedures are available in the SI.

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RESULTS AND DISCUSSION

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3.1 Efficiency of the single stage reactor configuration

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3.1.1 Period 1. At the beginning of period 1, the biomass showed a typical feast and famine

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response. The duration of the feast phase was approximately 16% of the total cycle duration.

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At the completion of the aerobic phase, the ammonium conversion to nitrite was 36±13%,

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producing only 6.5 mgNO2-N/L. Figures 2a and 2b shows the ammonium, nitrite, nitrate,

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OUR VFAs and PHAs profile for period 1. The OUR profile showed a dramatic decrease

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once the VFAs were depleted. On one hand this showed that the famine period was reached

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and also that the nitrification rate was very low. The main electron acceptor available during

275

the feast conditions was oxygen, while nitrite was the only electron acceptor present during

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the famine period. In this period, steady state conditions were not achieved, which is pointed

277

out by the high variation (higher than 50%) of the average –qVFA during feast conditions

278

(Figure 4 and Table 2). Despite the relatively high DO concentration during the aerobic phase,

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the nitrification activity of the biomass seems to be negatively affected by the presence of

280

VFAs during the feast phase, since the sAUR decreased from the initial value of 15-18

281

mgN/gXa h (measured in the inoculum) to 4.18±1.93 mgN/gXa h within 15 days of operation.

282

This is likely due to the faster biomass growth rate of VFAs consuming heterotrophic

283

organisms as opposed to nitrifying autotrophs27. As a consequence, the ratio between the

284

PHA stored (COD based) and the nitrite concentration at the beginning of the anoxic phase

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was 15.5 gCODVFA/gNO2-N, which was too high to allow for complete PHA degradation

286

under famine conditions. The lack of nitrite availability under anoxic conditions resulted in

287

poor nitrogen removal efficiency for this period (Table 2). Under denitrifying famine

288

conditions, the efficiency of PHA consumption was not more than 41±1% due to the shortage

289

of nitrite as electron acceptor (Figure 4). As a result, the PHA accumulated in the selection

290

reactor up to 0.21 gCODPHA/gCODX (Figure 2b). Maintaining efficient famine conditions,

291

where the stored PHA is consumed, has been found to be of high importance in achieving a

292

selected culture with a high PHA storage capacity24. During the first period, the –qVFA

293

decreased down to 4 mgCOD/gXa h (day 28, Figure 4) and the YPHA/VFA from 0.31 to 0.07

294

gCOD/gCODVFA (Figure 4). To conclude, the process of PHA biomass selection and nitrogen

295

removal via nitrite was not sustainable due to the low availability of nitrite, and the resulting

296

low degradation of the PHA during the famine period.

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To promote the growth of the PHA storing bacteria during the anoxic/famine conditions, the

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ammonium conversion to nitrite was enhanced by doubling the duration of the aerobic phase,

300

while maintaining constant the applied vNLR and the total vOLR (days 29 to 50). The

301

amount of nitrite increased up to 26 mgNO2-N/L and the nitrogen removal efficiency was

302

70.1%. The higher duration of the aerobic phase favoured the degradation of the PHA up to

303

66% (Figure 4, day 39) under famine conditions, due to the more abundant presence of

304

electron acceptors. However, it was found that ~30% of the previously stored PHA was

305

consumed under aerobic/famine conditions before the anoxic-famine period initiated. The

306

increase in the aerobic period resulted in an aeration time that exceeded the feast phase

307

duration for the applied vOLR, leading to some aerobic PHA degradation. The PHA content

308

at the end of the anoxic famine conditions was constantly below 0.7 % (gPHA/gVSS).

309

Furthermore, at the end of period 1 (days 29-50), the –qVFA increased together with the

310

YPHA/VFA up to 110±63 mgCOD/gXa h and 0.23±0.01 gCODPHA/gCODVFA (Figure 4),

311

respectively.

312

3.1.2 Period 2. Once the duration of the feast and famine phases were maintained relatively

313

stable, with deviations in the order of 5-7% from the average value for at least 12 consecutive

314

days (corresponding to one SRT), the vNLR was increased to 380±29 gN/m3d in order to

315

enhance the treatment capacity of the system, while maintaining constant the vOLR. Slight

316

alterations of the duration of the aerobic reaction were implemented in order to ensure

317

sufficient conversion of ammonium to nitrite, and to control the CODPHA/NO2-N ratio

318

between 2.0 and 2.2 gCODPHA/gNO2-N at the start of the anoxic/famine phase. In this way,

319

the effects of changes in temperature and in reject water were counterbalanced. More

320

specifically, aerobic conditions were maintained for 45±12% of the total cycle duration. The

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sAUR slightly increased up to 5.5±0.5 mgN/gXa h during the aerobic conditions, and the

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nitrite concentration was 32±12 mgNO2-N/L. As a result, more pronounced PHA production

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and consumption were observed in the feast and famine phases, respectively, during days 51-

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108 (Figure 2 c and d). During the last 33 days (days 75-108) of period 2, pseudo-steady state

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conditions prevailed, where the average –qVFA was 197±9 mgCOD/gXa h (Figure 4),

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resulting in the decrease of the ratio of the feast to the total cycle length from 0.19-0.20

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(period 1) to 0.15-0.18 (period 2). The higher capacity of the biomass to store PHA was

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confirmed by the YPHA/VFA, which increased from 0.23±0.01 (period 1) to 0.40±0.01 (period

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2) gCODPHA/gCODVFA (Figure 4). Furthermore, more than 80% of the PHA was degraded

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during the aerobic and anoxic famine conditions (days 87-108, Figure 4). Although the

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denitritation efficiency of the nitrogen available for denitrification was always higher than

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94%, the average nitrogen removal was still very low (48.8±3.4%, average value, see SI)

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since significant residual ammonium concentration remained. The performance of the process

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would clearly be improved if the ammonium conversion to nitrite increased. Thus, an

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alternate process configuration (Configuration 2) was studied in order to increase nitrogen

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removal and augment PHA production further.

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(Figure 2a, b, c, d)

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(Figure 3a and 3b)

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(Figure 4)

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3.2 Efficiency of the configuration 2 with two separate reactors

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To cope with the residual ammonium concentration, in configuration 2 a two stage process

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was applied for nitritation and selection of PHA storing biomass. Ammonium was first

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oxidized in the N-SBR (Figure 1b) and then the liquid was fed in the selection reactor when

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the anoxic reaction phase started. Figure 3 (a and b) shows the typical profiles of ammonium,

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nitrite, PHA, OUR and VFA concentration that were obtained in the selection SBR after

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adopting configuration 2 (day 109 to 150). The period of higher stability of the process was

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achieved during days 125-150, where pseudo-steady-state conditions were considered due to

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the low variation of the average –qVFA, qPHA and YPHA/VFA during the feast phases. In this

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period, the VFA were completely depleted within the first 30 min of the aerobic phase,

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resulting in a –qVFA of 239±7 mgCOD/gXa h (Figure 4). Under feast conditions, the PHA of

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the biomass rapidly increased at a qPHA of 89±7 mgCOD/gXa h, which is higher compared

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to the respective value of configuration 1. The average YPHA/VFA of 0.42±0.04

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gCODPHA/gCODVFA was similar compared to Period 2 of the previous configuration (Figure

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4). However, in Configuration 2, a similar YPHA/VFA was accomplished with a substantially

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higher vOLR (1394±112 gCOD/m3d), highlighting the advantage of operating with a separate

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reactor for nitrification and PHA biomass selection. After 50 min of aerobic phase, the

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anoxic-famine phase started by switching off the blower and feeding the effluent of the N-

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SBR. In configuration 2, nitrite was the only electron acceptor for the PHA degradation for

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denitritation during the famine conditions. Furthermore, this strategy allowed a better control

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of the applied CODPHA/NO2-N, avoiding nitrite limitation in the famine phase and enabling

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higher nitrogen removal efficiency (Table 2). Figure 3a shows the increase of the nitrite

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content at the start of the anoxic phase (up to 75 mgNO2-N/L). At the end of the anoxic phase,

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the PHA was consumed, reaching a minimum concentration of 0.6% (gPHA/gVSS) at the

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end of the anoxic cycle. This fact indicates that almost all the stored PHAs were degraded.

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The PHA consumption efficiency was high and stable during the days 118 to 150 (86±4%,

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Figure 4). Additionally, during the anoxic famine phase, the ammonium nitrogen decreased

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from 28 to 21 mgNH4-N/L; this reduction was correlated with the growth of the PHA storing

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bacteria. The rate of PHA degradation during the famine phase has been found to be

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independent of the type of electron acceptor present16. The famine (anoxic) duration and the

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carbon stored were enough to achieve almost 90% of denitritation. The nitrite at the end of

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the cycle decreased to 15 mgNO2-N/L. However, the nitrite concentration in the effluent was

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7-10 mgNO2-N/L, indicating that denitritation also occurred during the sedimentation but

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without any rising of sludge. This was confirmed by the low content of suspended solids in

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the treated effluent (