Diels-Alder approaches to model compounds related to

Jonathan C. Evans, Russell C. Klix, and Robert D. Bach. J. Org. Chem. , 1988, 53 (23), pp 5519–5527. DOI: 10.1021/jo00258a022. Publication Date: Nov...
1 downloads 0 Views 1MB Size
5519

J. Org. Chem. 1988,53, 5519-5527

Diels-Alder Approaches to Model Compounds Related to Fredericamycin A Jonathan C. Evans, Russell C. Klix, and Robert D. Bach* Department of Chemistry, Wayne State University, Detroit, Michigan 48202 Received M a y 4, 1988

A series of model compounds related to the antitumor and antibiotic compound fredericamycin A has been prepared. Spiro[3-cyclopentene-l,l’-indan]-2,5-dione(2) has been prepared and established as a novel spiro dienophile in the Diels-Alder reaction with 1,3-butadiene, Danishefsky’s diene, a triacetoxy-substituted oquinodimethane, and two isobenzofuran intermediates. Thus, the cycloaddition of 3-cyano-4,5,7-trimethoxy1(3H)-isobenzofuranone (35) and 2 afforded 4,9-dihydroxy-5,6,8-trimethoxyspiro[2~-benz[~indene-2,1’indan]-1,3-dione (38) in 62% yield. This methodology provides a viable synthetic route to the quinone portion of fredericamycin A that contains the seven requisite oxygens.

Fredericamycin AI is unique among recently isolated antitumor and antibiotic compounds in that it contains a spiro ring system (Figure 1). This hexacycle is the most active of several compounds produced by a soil bacterium of the streptomyces griseus family, a class of bacteria that produces many other antibiotics, including the tetracyclines and streptomycin. Since its isolation and characterization in 1981, a variety of synthetic approaches have been reported,2 including one successful total ~ y n t h e s i s . ~ Our synthetic strategy for the construction of the one (spiro) quaternary center of asymmetry in this quinoneisoquinolone containing molecule embodies a spiroexpansion-spiroannulation reaction involving an acyl migration (Scheme I).%J The principal purpose of the present paper is to demonstrate the utility of spiro[3-cyclopentenel,l’-indan]-2,5-dione (2) as a novel dienophile in the

Scheme I 0

5 ( 59% )

4

@ 0

2 preparation of fredericamycin A analogues. It is well known that the 2-cycloalkenones are poor dienophiles in the thermal Diels-Alder reaction? although Lewis acids do improve their r e a ~ t i v i t y .In ~ ~principle, the increased conjugation due to a second carbonyl group on the double bond should lower the LUMO in an enedione such as 2 relative to a simple cyclopent-2-en-1-one and markedly ~~~~

~

(1) (a) Pandey, R. C.; Toussaint, M. W.; Stroshane, R. M.; Kalita, C.

C.; Aszalos, A. A,; Garretaon, A. L.; Wei, T. T.; Byme, K. M.; Geoghegan, R. F., Jr.; White, R. J. J. Antibiot. 1981, 34, 1389. (b) Warnick-Pickle, D. J.; Byme, K. M.; Pandey, R. C.; White,R. J. J.Antibiot. 1981,34,1402. (2) (a) Ram0 Rao, A. V.; Reddy, D. R.; Deshpande, V. H. J. Chem. SOC.,Chem. Commun. 1984, 1119. (b) Parker, K. A.; Koziski, K. A.; Breault, G. Tetrahedron Lett. 1985,26, 2181. (c) Kende, A. S.; Ebetino, F. H. Tetrahedron Lett. 1985,26, 3063. (d) Eck, G.; Julia, M.; Pfeiffer, B.; Rolando, C. Tetrahedron Lett. 1985,26,4723. (e) Eck, G.; Julia, M.; Pfeiffer, B.; Rolando, C. Ibid. 1985,26, 4725. (f) Bach, R. D.; Klix, R. C. J. Org. Chem. 1986, 51, 749; 1985, 50, 5438. (9) Braun, M.; Veith, R. Tetrahedron Lett. 1986, 27, 179. (h) Bennett, S. M.; Clive, D. L. J. J. Chem. SOC.,Chem. Commun. 1986, 878. (i) Bach, R. D.; Klix, R. C. Tetrahedron Lett. 1986, 27, 1983. 6 ) Mehta, G.; Subrahmanyam, D. Tetrahedron Lett. 1987, 28, 479. (k) Clive, D. J.; Gagton, Angoh, A.; Bennett, S. M. J.Org. Chem. 1987,52, 1339. (1) Parker, K. A.; Breault, G. A. Tetrahedron Lett. 1986,27,3835. (m) Parker, K. A.; Spero, D. M.; Koziski, K. A. J. Org. Chem. 1987,52, 183. (n)Ram0 Rao, A. V.; Reddeppa Reddy, R.; Annapurna, G. s.;Deshpande, V. H. Tetrahedron Lett. 1987,28,451. (0)Ram0 Rao, A. V.; Shreenivassan, N.; Reddeppa Reddy, R.; Deshpande, V. H. Ibid. 1987,28,455. (p) Clive, D. L. J.; Sedgeworth, J. J.Heterocycl. Chem. 1987,24,509. (9) Domagala, J. M.; Bach, R. D. J. Am. Chem. SOC.1979,101, 3118. (3) Kelly, T. R.; Ohashi, N.; Armstrong-Chong, R. J.; Bell, S. H. J. Am. Chem. Sac. 1986,108, 7100. (4) (a) Fringuelli, F.; Pizzo, F.; Taticchi, A.; Halls, T. D. J.; Wenkert, E. J. Org. Chem. 1982,47, 5056. (b) Fringuelli, F.; Pizzo, F.; Taticci, A,; Wenkert, E. J. Org. Chem. 1983,48, 2802. (c) Agosta, W. C.; Smith, A, B. I11 J. Org. Chem. 1970, 35, 3856.

improve its capacity to function as a dieneophile. An earlier report suggested that the 2,2-dimethyl derivative of the parent enedione was a relatively sluggish dieneophile as a result of steric interaction^.^^

Results and Discussion Dienophile 2 was prepared by an adaption of the Kuwajima5 annelation procedure. We chose to utilize a thioketal and a mercuric salt as the thiophile since we have found these reaction conditions to be more compatible with other functionality in the molecule. This procedure also greatly simplified both the workup and the isolation of the 1,3-dione resulting from acyl migration. The benzo-substituted spiro[4.4]nonane-1,4-dione 6 needed to prepare dienophile 2 was synthesized under unusually mild reaction conditions by utilizing a mercury-mediated 1,2-carbonyl migration. The overall reaction sequence proved to be a generally effective procedure for the introduction of spiro centers. As outlined in Scheme I, the acyloin condensation of diethyl succinate in the presence of chlorotrimethylsilane afforded 1,2-bis(trimethylsilyloxy)cyclobutene (3).6 (5) (a) Nakamura, E.; Kuwajima, I. Organic Synthesis; Vedejs, E., Ed.; Wiley: New York, 1987; Vol. 65, p 17. (b) Kuwajima, I.; Shimad, J.; Hashimoto, K.; Kim, B. H.; Nakamura, E. J.Am. Chem. SOC.1984,106, 1759. (c) Kuwajiua, I.; Azegami, I. Tetrahedron Lett. 1979, 2369. (d) 1977, 99, 961. (e) KuNakamura, E.; Kuwajima, I. J. Am. Chem. SOC. wajima, I.; Hashimoto, K.; Nakamura, E. J. Org. Chem. 1977,42,4166. (6) (a) Bloomfield, J. J.; Owsley, D. C.; Nelke, J. M. Org. React. (N.Y.) 1976, 23, 259. (b) Bloomfield, J. J.; Nelke, J. M. Organic Synthesis; Johnson, C. R., Ed.; Wiley: New York, 1977; Vol. 57, p 1. (c) Bloomfield, J. J. Tetrahedron Lett. 1968, 587.

0022-3263/88/1953-5519$01.50/00 1988 American Chemical Society

J. Org. Chem., Vol. 53, No. 23, 1988

5520

Evans et al. Scheme I1

SYH3

HO

2 1

Figure 1. Fredericamycin A (1).

Treatment of 1-indanone and ethanethiol in methylene chloride at -40 "C with stannic chloride afforded 1,l-bis(ethy1thio)indan (4) in 74% yield. cy-Thioalkylationof 4 was readily achieved under the influence of the relatively mild Lewis acid, boron trifluoride etherate.' Reaction of thioketal 4 with boron trifluoride etherate and bis(sily1 ether) 3 in methylene chloride at -40 O C afforded thioindanyl cyclobutanone 5 in 59% yield after purification by column chromatography. The pinacol-type rearrangement of 5 to 6 was readily achieved by the action of the mild thiophile mercuric chloride in refluxing benzene. Stereospecific acyl migration may also be smoothly effected by the more electrophilic Lewis acid mercuric trifluoroacetate in benzene at 0 OC.% Mechanistic rational for this rearrangement is depicted by eq 1. Thus, treatment of

f-x

0

0

cyclobutanone 5 with mercuric chloride in refluxing benzene afforded a 91% yield of spiro 2,5-dione 6. The preparation of dienophile 2 was completed by a roomtemperature bromination-dehydrobromination procedure in 68% yield. As anticipated, the Diels-Alder cycloaddition of 1,3butadiene with 2 afforded adducts 7 and 8 (5:l) in a 75% yield (eq 2). The structural assignments given 7 and 8 +

,&

Me0 OSiMe3

9a

9b ( 6 : l ) (87%)

1

Silica gel

Meb

10 ( 60% overall ) are consistent with those given to the same pair of diastereomers prepared by an alternative method.2f The assigned stereochemistryzf of 7 and 8 is based upon the assumption that 1,2-carbonyl migrations are stereospecific reactions that proceed with inversion of configuration at the migration terminus (eq 1).%The Diels-Alder reaction of dienophile 2 with Danishefsky's dienes in benzene under an argon atmosphere also afforded a mixture of diastereomers 9a and 9b (87%) in 6 h (Scheme 11). The trimethylsilyl group ('H NMR, 0.23 ppm) was lost upon chromatographicworkup on silica gel, affording 1,3,5-trione 10 as a mixture of stereoisomers (mp 185-186 O C ) in 60% yield. As one would anticipate, the "electron-rich" diene underwent a Diels-Alder cycloaddition with 2 at a reduced temperature and reaction time in comparison to 1,3-butadiene. Reaction of the more hindered diene with dienophile 2 afforded a 6:l ratio of diastereomers 9a and 9b, which is consistent with the assumption that the two reactants will approach each other from the least sterically hindered direction. In a preliminary report we described the room-temperature Diels-Alder cycloaddition reaction of dienophile 2 with an o-quinodimethane intermediate (eq 3). o-

120"C, 20 h C6H6

0

25" C, 2h

2

7

8 ( 5 : l ) (75%)

(7) Reetz, M. T.; Giannis, A. Synth. Commun. 1981,11, 315.

( 94%

1

Quinodimethane derivatives are very reactive in the (8) Danishefsky, S.; Kitahara, T.; Schuda, P. F. Organic Synthesis; Stevens, R. V., Ed.; Wiley: New York, 1983; Vol. 61, p 147.

J . Org. Chem., Vol. 53,No. 23, 1988 5521

Model Compounds Related to Fredericamycin A Scheme I11

Scheme IV

4.H2Br Zn (

AcO

DMF, rt

OAc

13

12

CH2Br

Act. )

16

17

t

DMF, 2 h rl

OAc

OAC

f-$@ 0

hv

AcO

CH2Br OAc

NBS,

2 cc’4

reflux

ACO OAc

*

612,

AcOH

Diels-Alder reaction because of the restoration of aroOAc OAc maticity upon cycloaddition. These diene intermediates have been utilized extensively for the synthesis of poly19 ( 74% ) 18 ( 54% 1 cyclic ring systems that are otherwise difficult to ~ r e p a r e . ~ Intrigued by the ease of reaction of dienophile 2 with this dienophile 2 and zinc (freshly activated) in N,N-dihighly reactive intermediate (eq 31, we turned our attention methylformamide with substituted o-(a,a’-dibrom0)xylene to the development of an o-quinodimethane derivative with 16 at room temperature afforded spiro dione 18 in 54% latent functionality, that could potentially introduce the yield. The o-(cup’-dibromo)xylene 16 was added to the required oxygen functionality in 1. We looked at possible dienophile to avoid dimerization of the highly reactive precursors to the oxygen functionalized diene 11 and chose o-quinodimethane intermediate 17. Diels-Alder adduct the dihalide 16. Our approach to this compound is shown 18 was aromatized with bromine and acetic acid, prior to in Scheme 111. Following the procedure of Nilsson,lo attempting to introduce the remaining two oxygens of the quinone portion of fredericamycin A. Several attempts were made to oxidize the middle ring of 19 with chromium trioxide in an 80% acetic acid solution. Unfortunately, we recovered only 19 after workup. The inability to readily oxidize the 4- and 9-positions of 19 is attributed to deactivation by the carbonyl groups of the adjacent cyclopentane ring. 11 Seeking a method in which we could introduce oxygen in the middle ring of the quinone portion of fredericamycin phenol 12 was coupled with diazotized sulfanilic acid, and A by a Diels-Alder cycloaddition reaction, we examined the red azo compound was reduced with sodium dithionite the possibility of using 3-cyano-1(3H)-isobenzofuranone to the aminophenol 13. Aminophenol 13 was converted as a diene precursor. The literature contains a number to 2,3-dimethylbenzoquinone(14) by reaction with ferric of reports of cyclophthalides (3-cyano-l(3H)-isobenzochloride and concentrated hydrochloric acid. At this point, furanones) undergoing l,.l-additions to enones in a Michael we wanted to introduce the third oxygen functionality into fashion but is devoid of any examples of cyanophthalides quinone 14, which could later be transformed into the being added to dienophiles under Diels-Alder reaction methoxy group that is present in fredericamycin A. conditions. Morrow and Swenton13 have annulated a Treatment of 14 with boron trifluoride etherate, with acetic highly functionalized quinone monoketal with a substianhydride as the solvent, effected the Thiele’l reaction, tuted cyanophthalide anion in their synthesis of adriawhich afforded the triacetoxy derivative 15 in a 72% yield mycin derivatives. More recently, Yoshii et al.14 utilized after recrystallization. Light-catalyzed NBS bromination an acyclic cyanophthalide anion in an annulation reaction of 15 afforded (77%) the corresponding a,a’-dibromo dewith 5-tert-butoxy-2-furfurylideneacetonein their synrivative 16. thesis of (f)-granaticin. In both of these syntheses the Our attention then turned to seeking a reagent that functionalized cyanophthalide anions have been added to would be compatible with the carbonyl groups in the enones in a Michael fashion, followed by addition of the dienophile 2 and suitable for the generation of o-quinoresulting enolate anion of the intermediate to the carbonyl, dimethane intermediate 17 (Scheme IV). We chose acwith ring opening and subsequent loss of cyanide. Utilizing tivated zinc12 to generate o-quinodimethane 17 in situ, Hauser’s’j 1,Qdipolar equivalent 20, Parker2bhas reported which readily afforded adduct 18 upon Diels-Alder cythe synthesis of 2,2-dimethy1-4,9-dihydroxy-lH-benz[flcloaddition with dienophile 2 (Scheme IV). Reaction of indene-l,3(2H)-dione (21, eq 4). In an analogous manner, annulation of 3-cyano-1(3H)-isobenzofuranone(22) to ene (9)(a) Ito, Y.;Nakatauka, M.; Saegusa, T. J. Am. Chem. SOC.1982, dione 2 would provide the three contiguous rings of the 104,7609. (b) Ardecky, R. J.; Dominguez, D.; Cava, M. P. J. Org. Chem. quinone half of 1, as well as the introduction of the ap1982,47,409.(c) Kerdesky, F.A. J.; Ardecky, R. J.; Lakshmikantham, M. V.; Cava, M. P. J. Am. Chem. SOC.1981,103,1992.(d) Kerdesky, F. propriate oxygen functionality to the middle ring. Anionic

r

A. J.; Cava, M. P. J. Am. Chem. SOC.1978,100,3635.( e ) Cava, M. P.; Deana, A. A.; Muth, K. J. Am. Chem. Soe. 1959,81,6458. (10) Nilsson, J. L. G.; Sievertsson, H.; Selander, H. Acta Pharm. Suec. 1968,5, 215. (11)Katauhara, J. J. Org. Chem. 1967,32,797. (12)Alder, K.; Fremery, M. Tetrahedron 1961,14,190.

(13)Morrow, G.W.;Swenton, J. S. J. Org. Chem. 1987,52, 713. (14)Nomura, K.; Okazaki, K.; Hori, K.; Yoshii, E. J. Am. Chem. SOC. 1987,109, 3402. (15)Hauser, F.M.;Prasanna, S. J. Am. Chem. SOC.1981,103,6378.

5522 J. Org. Chem., Vol. 53, No. 23, 1988 S02Ph

Evans et al. Scheme V

-

1. t-BuLi/THF, -78" C

1 , 2.3 equiv. n-BuLi

diisopropylamine

0

n

THF, 0°C

Et0

OEt

2. TMSC'

OEt

27

26

OH

"

2 1 ( 48%

)

addition of cyanophthalide16 (22) to enedione 2 afforded an 80% yield of spiro dione 23 as a white crystalline material (eq 5). Even though the addition of the lithium

@ 0

1. 3.2 equiv t-BuLi

THF, -78°C

0

22

2.

1%r. TFA, t., 20CHC13 min

@ 0

0

0

0

OEt

29 ( 94% )

m

:$lQ

0 OEt

28 ( 6 8 % )

aCozH

(2)

Scheme VI

-78°C to 25"C, 2 h

1 NaOH/HzO

NaOHIH20

HO

(MeOhSOz

(eq. 5)

&CozH

Me0

KzS208

2. HCI

Me0

NazSOI

2h

HO

30 (56%)

31 (30%)

6H

23

/

( 80% )

Acetone

2. KOH/H20

enolate of cyanophthalide to 2 is a well-behaved reaction that proceeds in high yield, we felt that the use of tertbutyllithium could ultimately cause problems when added to ene dione 25 in the final stages of a total synthesis of 1. As a result, we deemed it advisable to develop a Diels-Alder route under neutral reaction conditions that would allow the preparation of the quinone portion of fredericamycin A.

1. sec-BuLi 1 2 . %LHCl,

AcOH

1. KCNIHzO

Me0

2 HCI

Me0

34 ( 47% )

0

25 Rickborn et al." have recently reported the f i s t example of an alkoxyisobenzofuran-aryne cycloaddition. 1-Ethoxy-3-(trimethylsilyl)isobenzofuran(27) was generated in situ and shown to undergo Diels-Alder reactions with substituted benzynes (arynes). Utilization of the transient 1-ethoxyisobenzofuran in a cycloaddition reaction with enedione 2 would establish the credibility of this DielsAlder route to the quinone portion of fredericamycin A. Reaction of 27 with dienophile 2 afforded bridgehead trimethylsilylated ketal 28 (Scheme V). Thus, treatment

Me0

OH

35

( 93%

1

of l,l-diethoxyphthalan's (26) and diisopropylamine in tetrahydrofuran at 0 "C with n-butyllithium resulted in a yellow solution. The mixture was stirred at 0 "C for an additional hour to ensure complete anion generation, and the anion was quenched with chlorotrimethylsilane. A solution of 27 in tetrahydrofuran was then added to dienophile 2. The mixture was warmed to room temperature and stirred for 24 h. Concentration and purification by flash chromatography on silica gel with 20% ether in hexane, with 1% triethylamine to neutralize the column, afforded Diels-Alder adduct 28 in 68% yield. Catalytic trifluoroacetic acid cleavage of 28 at the ketal center, followed by facile Brook rearrangement, afforded 29 in a 94% yield. Since we have established that spiro enedione 2 was reactive toward isobenzofuran 27 i t now remained to prepare the precursor leading to the fully oxygenated

(16) Freskos, J. N.; Morrow, G . W.; Swenton, J. S. J.Org. Chem. 1985, 50, 805.

(17) (a) Mirsadeghi, S.; Rickborn, B. J. Org. Chem. 1987,52, 787. (b) Pollart, D. J.; Rickborn, B. J . Org. Chem. 1987, 52, 792.

(18) (a) Contreras, L.; MacLean, D. B. Can.J . Chem. 1980,58, 2573. (b) Contreras, L.; MacLean, D. B. Can. J . Chem. 1980,58, 2580.

J. Org. Chem., Vol. 53, No. 23, 1988 5523

Model Compounds Related to Fredericamycin A Scheme VI1 1 . 1-BuLi, -78°C

THF

Me0 OMe C N

2. TMSCl -78°C io 25°C

L

OMe C N

35

36

0 -78'C 10 25% 6h

1

*

Me0

Silica gel Me0

was added, and t h e mixture was warmed t o room temperature. To the reaction mixture a t -78"C a solution of dienophile 2 (1.1 equiv) in tetrahydrofuran was added. T h e resulting red solution was warmed to room temperature and stirred for 6 h t o afford crude 37. The trimethylsilyl group ('H NMR, 0.18ppm) was lost upon chromatographic workup on silica gel using hexane-ethyl acetate (1:l). Recrystallization (chloroform-hexane) of the chromatographed product afforded crystalline 38 in 62 % yield. This Diels-Alder cycloaddition reaction has allowed us to successfully introduce the seven oxygens of the quinone portion of fredericamycin A. Selective demethylation of 38 will produce 4,9-dihydro-6-methoxy-spiro[2H-benz[flindene-2,1'-indan]-l,3,5,8-tetr0ne.~'The application of this synthon to the total synthesis of fredericamycin A is presently under way.

Experimental Section

General Procedures. All moisture-sensitive reactions were Me0 OH conducted in flame- or oven-dried apparatus under a positive pressure of argon. 'H and 13CNMR spectra were recorded at 300 37 and 75 MHz, respectively. All solvents were dried and purified 38 ( 62% ) before use when required. 1,2-Bis[(trimethylsilyl)oxy]cyclobutene (3). Via the proisobenzofuran. With the isolation of 28. we felt that with cedure of Bloomfield,G cyclobutene 3 was prepared in a 75% yield the proper manipulation of this methodology we could bp 74 "C (7 mm); 13C NMR (CDClJ 120.1, 26.0, 0.314 ppm; 'H introduce all five remaining oxygens of the quinone portion NMR (CDCl,) 8 0.03 (s, 18 H), 1.98 (s,4 H); IR (neat) 1719, 1311, in one step. The synthesis of isobenzofuranone 35, which 1252, 878, 847 cm-'. contains t h e remaining five oxygens needed to completely 1,l-Bis(ethy1thio)indan (4). Stannic chloride (19 mL, 0.16 functionalize the quinone portion of fredericamycin A, is mol) was added dropwise over a 30-min period to a solution of outlined in Scheme VI. Selective methylation of com1-indanone (73.9 g, 0.56 mol) and ethanethiol (83.8 mL, 1.13 mol) in methylene chloride (500 mL) at -78 "C. The reaction mixture mercially available 2,4-dihydroxybenzoic acid was accomwas stirred at -78 "C for 3 h and then allowed to warm to -40 plished with 20% sodium hydroxide and dimethyl sulfate. "C before being poured into a saturated solution of sodium biElbs persulfate oxidation converted 30 t o 2,5-dihydroxycarbonate (700 mL). The organic layer was separated, and the 4-methoxybenzoic acid (31) in modest yield. Methylation aqueous layer was extracted with methylene chloride (3 X 500 of 31 under s t a n d a r d conditions a n d saponification afmL). The combined organic extracts were washed with water (3 forded commercially available 2,4,5-trimethoxybenzoic acid X 1L), saturated aqueous sodium chloride (2 X 800 mL),and dried (32) in 76% yield. Treatment of 32 with thionyl chloride (MgS04). Concentration resulted in an oil, which was further in benzene afforded a 98% yield of crude 2,4,5-trimethpurified by column chromatography on silica gel with hexane and oxybenzoyl chloride which was converted t o N,N-diethyl acetate (19:l) to afford, after trituration from pentane, 98.9 ethyl-2,4,5-trimethoxybenzamide (33) in 93% yield, using g (74%) of 4 as a white crystalline material: mp 31-33 "C; 13C NMR (CDC13) 144.4, 142.4, 127.9, 126.1, 125.1, 123.7,66.1,43.0, ~ diethylamine in benzene. Selective o r t h o - l i t h i a t i ~ n 'of 30.3, 24.8, 13.8 ppm; 'H NMR (CDCI,) 6 1.15-1.20 (SCH2CH3, 33 and subsequent trapping with N,N-dimethylformamide resulted in N,N-diethyl-6-formyl-2,4,5-trimethoxybenz- t, J = 15.0 Hz, 6 H), 2.43-2.68 (m, 6 H), 2.97-3.02 (t, J = 15.0 Hz,2 H), 7.19-7.25 (m, 3 H), 7.31-7.34 (t, J = 9.0 Hz,1 H); IR amide, which upon treatment with 10% hydrochloric acid 3068, 3037, 2968 (vs), 2927 (vs), 2869, 1472 (s), 1455 (s), in acetic acid afforded 3-hydroxy-4,5,7-trimethoxy-l- (neat) 1447,1374,1258,1152,1057,1030,1022,976,876,810,765 (vs), (3H)-isobenzofuranone (34). T r e a t m e n t of 34 with po741, 735 cm-'. tassium cyanide in water, followed by concentrated hy2 4 I-(Ethy1thio)indan- 1-y1]-2-[(trimethylsilyl)oxy]cyclodrochloric acid, afforded isobenzofuranone 35 in 93% yield. butanone ( 5 ) . To a solution of 23.8 g (0.10 mol) of 1,l-bisDiels-Alder cycloaddition of isobenzofuranZ036, gen(ethy1thio)indan (4) in methylene chloride (100 mL) at -60 "C was added 37.0 mL (0.30mol) of boron trifluoride etherate. After erated in situ, and enedione 2 afforded, after flash chrothe addition of the Lewis acid, 25.4 g (0.11 mol) of cyclobutene matography, the desired model spiro adduct 38 in 62% 3 in methylene chloride (40 mL) was added dropwise. The mixture yield (Scheme VII). In a typical reaction sequence, 35 in was allowed to warm to -40 "C and stirred for 1 h. The reaction tetrahydrofuran at -78 "C was treated with 1.0 equiv of mixture was poured at -40 "C into a saturated solution of sodium a tert-butyllithium solution. T h e solution was stirred a t bicarbonate (400 mL). The organic layer was separated, and the -78"C for 30 min before 1.2 equiv of chlorotrimethylsilane aqueous layer was extracted with methylene chloride (3 X 200 mL). The combined organic phases were washed with water (2 X 400 mL) and saturated sodium chloride solution (1 X 400 mL) (19) Beak, P.; Meyers, A. I. Acc. Chem. Res. 1986,19,356. and dried (MgSO,). Concentration of the organic phase resulted (20) Generation and 'H NMR spectral characterization of intermein an oil, which was purified by flash chromatography on silica diate 3 4 to dimsyl anion in deuteriated dimethyl sulfoxide at -78 OC was added a solution of 33 in deuteriated dimethyl sulfoxide. After the gel with hexane and ethyl acetate (29:l) to afford 19.7 g (59%) mixture was stirred at -78 OC for 10 min, chlorotrimethylsilane was of 5: 13C NMR (CDC1,) 211.4, 145.0, 142.5, 127.8, 126.1, 125.2, added. The reaction mixture was stirred at -78 O C for 30 min before 124.6,99.0,63.5,42.1,34.9,31.3,26.0,24.0,13.9,1.34 ppm; 'H NMR being allowed to warm to room temperature. The 'H NMR spectrum of (CDCl,) 8 0.031 (Si(CH3),,s, 9 H), 1.03-1.08 (SCH2CH3,t, J = this solution indicated complete loss of 33 and the formation of 315.0 Hz,3 H), 2.01-2.12 (m, 1H), 2.20-2.31 (m, 3 H), 2.65-2.85 cyano-4,5,7-trimethoxy-l-[(trimethylsilyl)oxy]isobenzofuran (34). 'H NMR spectra indicated the presence of a silane resonance at 0.188 ppm, (m, 4 H), 2.93-2.98 (t, J = 15.0 Hz, 2 H), 7.17-7.22 (m, 3 H), the downfield shift of the aromatic proton, and the disappearance of the resonance associated with the proton attached to the carbon bearing the cyanide. No attempt was made to isolate this very unstable isobenzofuran. Our spectral data for 34 is in good agreement with that reported by Pollart and R i ~ k b o r n for ' ~ ~l-ethoxy-3-(trimethylsilyl)isobenzofuran.

(21) Hauser, F. M.; Hewawasam, P.; Baghdanov, V. M. J . Org. Chem. 1988,53, 223. (22)Smith, L.I.; Tess, W. H. J . Am. Chem. SOC. 1944,66, 1523.

5524

J. Org. Chem., Vol. 53, No. 23, 1988

Evans et al.

reaction mixture was refluxed for 6 h before being allowed to cool to room temperature. Concentration yielded 520 mg (70%) of impure dione 9: 'H NMR (CDC13)6 0.229(s,9H), 2.35-2.60(m, 4 H),3.12-3.32(m, 4 H), 3.66(s,3H), 4.17-4.21(m, 1 H), 5.55-5.59 (d, 1 H), 6.85-6.88(d, 1H), 7.07-7.12(t, 1H), 7.26-7.32(t, 1 H), 7.55-7.60(d, 1 H). Purification by flash chromatography on silica gel with hexane and ethyl acetate (2:l)afforded 358 mg (60%) of trione 10: mp 178-179 "C (hexane-ethyl acetate); I3C NMR through a coarse sintered-glass funnel containing Celite. The (CDCl3) 214.2,210.8,205.8,145.4,141.3,128.4,126.8,125.7,122.1, organic layer was washed with 10% hydrochloric acid solution 78.8,69.4,56.5,51.1,43.4,41.5,35.0,32.7,31.7ppm; 'H NMR (1 X 50 mL), water (2X 50 mL), and saturated sodium chloride (CDCl,) 6 2.39-2.69(m, 4 H), 2.83-2.90(dd, J' = 20.4Hz, fi = solution (1 X 50 mL) and dried (MgS04). The solvent was re3.3Hz, 1 H), 3.14-3.32(m, 7 H), 3.62-3.71(dt, fl = 6.0Hz, SZ moved under reduced pressure to afford 4.89g (94%) of spiro= 3.0Hz, 1 H), 4.23-4.26(4, J = 3.0Hz, 1 H), 6.86-6.88(d, 1 H), [cyclopentane-l,l'-indan]-2,5-dione. Recrystallization from hexane 7.09-7.14(t, J = 9.0 Hz, 1 H), 7.19-7.24(t, J = 9.0Hz, 1 H), and ethyl acetate yielded 4.73g (91%) of 6 as a white solid: mp 7.27-7.31(d, 1 H); IR (KBr)3072,2990,2930,2882, 2834,1727 107-108 "C; 13CNMR (CDC13) 213.0,144.8,140.8,128.3,126.9, (vs), 1725 (vs), 1721 (vs), 1698,1478,1454,1290 (s), 1246 (s), 1194, 125.4,122.4,69.8,35.5,32.8,31.6 ppm; 'H NMR (CDCl,) 6 1002 (s),982,754(s) cm-'; MS (70eV) calcd for C&1,O4 298.1204, 2.37-2.42(t, J = 7.5Hz, 2 H), 2.82-2.95(m, 2 H), 2.95-3.09(m, found 298.1204. 2 H), 3.14-3.19(t,J = 7.5Hz, 2 H), 6.89-6.91(d, 1 H), 7.12-7.17 2,3-Dimethylbenzoquinone(14). By use of the procedure (t, J = 7.5Hz, 1 H), 7.20-7.25(dt, J = 7.2Hz, 52 = 0.9Hz, 1 H), of Nilsson,'O 2,3-dimethylbenzoquinone(14)was prepared in a 7.27-7.30(d, 1H); IR (KBr)3071,3021, 2980,2949,2936,2920, 2853,1717(vs), 1478,1456,1412,1271,1258,1194,1111,995,772 62% yield mp 55 "C (lit.*lmp 57-58 "C); 13CNMR (CDCl,) 187.0, 140.7,136.0,11.9ppm; 'H NMR (CDCl,) 6 2.03(s, 6 H), 6.72(9, (s)cm-'; MS (70eV) calcd for C13H12O2 200.0837,found 200.0837. 2 H); IR (KBr)3305,3271,3058,2959,2931,1764,1655,1635, Anal. Calcd for C13H1202: C, 77.98;H, 6.04.Found: C, 77.87; 1603,1311,1138,1065,834 cm-'. H, 5.89. 1,2,5-Triacetoxy-3,4-dimethylbenzene(15). To a solution Spiro[3-cyclopentene-1,1'-indan]-2,5-dione (2). To a solution of 19.7g (0.14mol) of 2,3-dimethylbenzoquinone(14)in acetic of 4.3g (22mmol) of spiro dione 6 in glacial acetic acid (100 mL) anhydride (200mL) at 0 "C was added 34.4mL (0.28mol) of boron at room temperature was added 1.10mL (22mmol) of bromine. trifluoride etherate. The reaction mixture was stirred for 3 h at The reaction mixture was allowed to stir for 1h and then poured room temperature. The reaction mixture was poured into water into water (100 mL). The aqueous layer was extracted with (500 mL) and extracted with methylene chloride (3 X 150 mL). methylene chloride (3 X 100 mL), and the combined extracts were The methylene chloride layer was then removed under reduced washed with water (1 X 300 mL), saturated sodium bicarbonate pressure to afford 32.6g (83%) of crude 3,4,6-triacetoxy-o-xylene solution (2x 200 mL), and saturated sodium chloride solution (15)as a white solid. Recrystallization of the solid from hexane (1 x 200 mL) and dried (MgS04). Removal of the solvent under and ethyl acetate afforded 30.3g (77%) of 15: mp 88-89 "C; 13C reduced pressure followed by flash chromatography on silica gel NMR (CDCl3) 168.7,168.0,146.3,140.1,138.6,131.7,127.6,114.5, using hexane and ethyl acetate (9:l)yielded 2.95g (68%) of 2 20.7,20.5,20.2,13.2,12.8ppm; lH NMR (CDCI,) 6 2.07(9, 3 H), as a yellow crystalline material: mp 80-81 "C (hexane); 13CNMR 2.11(s, 3 H), 2.24(5, 3 H), 2.29(s, 3 H), 2.30(s, 3 H),6.83(9, 1 (CDC13)204.2,150.2,145.5,140.2,128.5,126.9,125.2,122.2,63.2, H); IR (KBr)2999,2933,1775,1760,1478,1372,1190,1085,922, 31.9,31.7ppm; 'H NMR (CDCl,) 6 2.41-2.46(t, J = 7.3 Hz, 2 910cm-'; MS (70ev) calcd for Cl4Hl6O6280.0947,found 280.0951. H),3.23-3.28(t,J = 7.6Hz, 2 H), 6.79-6.82(d, 1 H), 7.12-7.15 1,2,5-Triacetoxy-3,4-bis(bromomethyl)benzene (16). A (t, J = 7.4 Hz, 1 H), 7.24-7.28(dt, J' = 7.5 Hz, SZ = 1.1 Hz,1 mixture of 2.8g (10mmol) of 3,4,6-trimethoxy-o-xylene (15),3.74 H), 7.33-7.35(d, 1 H), 7.51(s, 2 H); IR (KBr)3062,2977,2961, g of N-bromosuccinimide, and 5 mg of benzoyl peroxide in re2945,2865,1700 (vs), 1672 (s),1478 (s), 1458,1327,1284 (s), 1257, fluxing carbon tetrachloride (50 mL) was irradiated (GE sunlamp) 1214,863,836,789,750(9) cm-'; MS (70eV) calcd for C13H10O2 for 1h. Filtration of the reaction mixture afforded the product 198.0681,found 198.0684. and succinimide. The solid was dissolved in methylene chloride 3a,4,7,7a-Tetrahydrospiro[2H-indene-2,l'-indan]-lf-dione (75mL) and washed with a 3% sodium hydroxide solution (1X (7, 8). Dienophile 2 (500 mg, 2.5mmol), 1,3-butadiene (406mg, 50 mL). The organic layer was then washed with water (2X 50 7.5mmol), and benzene (3mL) were placed in a glass tube and mL) and a saturated sodium chloride solution (1X 50 mL) and sealed under vacuum. The reaction mixture was heated for 20 dried (MgS04). Concentration resulted in a white solid, which h at 120 "C. After the sealed tube had been allowed to cool to was recrystallized from hexane and ethyl acetate to afford 3.6g room temperature, it was cooled to -78 "C before being opened. (82%) of the bis(bromomethy1) derivative 16: mp 170-172 "C; The reaction mixture was concentrated to give a mixture of 7 13CNMR (CDCl,) 168.0,167.1,146.8,143.0,138.9,131.2,126.8, (83%) and 8 (17%) by 'H NMR. Distillation afforded 470 mg 118.9,21.9,21.7,20.8,20.6,20.2ppm; 'H NMR (CDCl,) 6 2.24 (75%), bp 144-160 "C (0.075mmHg), of a mixture of 7 (51%) (s, 3 H),2.26 (s, 3 H), 2.26 (s, 3 H), 4.54(CH2Br,s, 4 H), 7.19(s, and 8 (18%). Attempts to separate 7 from 8 by recrystallization 1 H); IR (KBr)3095,3047,1772(vs), 1761 (vs), 1471,1431, 1366, failed. Further purification was carried out by trituration with 1321,1209 (vs), 1182 (vs), 1155 (s), 1088, 1019,961,911,889,855, pentane and ether at -78 "C. 7: mp 110-112"C (lit."mp 111-112 764 cm-'. "C); 13CN M R (CDC13)214.8,145.0,141.7,128.4,127.0,126.1, 125.6, 5,6,8-Triacetoxy-3a,4,9,9a-tetrahydrospiro[2~-benz[f]122.3,68.7,45.5,33.6,32.2, 21.7ppm; 'H NMR (CDC13)6 2.23-2.42 indene-%,l'-indan]-l,3-dione (18). To a solution of 350 mg (1.8 (m, 4 H),2.36-2.40(t,J = 7.5Hz, 2 H), 3.16-3.21(t,J = 7.5Hz, mmol) of dione 2 and 208 mg (3.2mmol, 2.0equiv) of zinc (freshly 2 H),3.31-3.41(m, 2 H), 5.78-5.80(t,J = 2.1Hz, 2 H),6.93-6.95 activated) in NJV-dimethylformamide(2 mL) at room temperature (d, 1 H), 7.08-7.13(t, J = 7.5Hz, 1 H), 7.17-7.21(t,J = 6.0Hz, was added a solution of 717 mg (1.5mmol, 1.2equiv) of dibrom1 H),7.25-7.28(d, 1 H); IR (KBr)3035,2938,2848,1763,1721 oxylene 16 in N,N-dimethylformamide (10mL) dropwise over (vs), 1660,1478,1222 (s), 1205,1187,779,780,659cm-'. 8: 13C a 10-min period. The reaction mixture was stirred at room temNMR (CDC13) 215.3,146.3,140.2,128.5,127.1,126.2,124.8,124.2, perature for 2h and then poured into a beaker containing ice water 68.9,45.0,39.2,31.5,21.4ppm; lH NMR (CDC1,) 6 2.31-2.33(m, (50 mL). The resulting pink precipitate was collected by filtration. 2 H), 2.34-2.39(t,J = 8.0 Hz, 2 H), 2.48-2.55(m, 2 H), 3.06-3.11 The precipitate was dissolved in ethyl acetate (50mL), and any (t,J = 7.5Hz, 2 H), 3.28-3.33(m, 2 H), 5.82-5.83(t, J = 1.5 Hz, insoluble impurities were removed by Titration. The ethyl acetate 2 H), 6.85-6.87(d, 1 H), 7.10-7.18(t, J = 12.0Hz, 1 H), 7.19-7.23 was removed under reduced pressure, and the crude crystalline (dt, # = 6.0Hz, 52 = 1.5Hz, 1 H), 7.26-7.30(d, 1 H); IR (KBr) product was ground up in a beaker containing water. The product, 3034,2942,2901,2826,1766,1726 (vs), 1662,1483,1283 (s), 1199, which is insoluble in water, was then collected by filtration, 1184,1146,782,699 cm-'; MS (70eV) calcd for C17H1602 252.1150, dissolved in chloroform (40mL), and dried (MgS04). The drying found 252.1155. agent was removed by filtration through a medium sintered-glass 3a,4,6,7a-Tetrahydro-7-methoxyspiro[2H-indene-2,1'filter containing Celite (to remove any remaining traces of zinc). indan]-1,3,5-trione(10). To a solution of 400 mg (2mmol) of Concentration yielded 624 mg (84%) of crude 18,which was spiro[3-cyclopentene-l,l'-indan]-2,5-dione (2) in benzene (2mL) further purified by recrystallization with chloroform, ether, and of Danishefsky's8diene. The at 25 "C was added 520 mg (3"01)

7.36-7.40(m, 1 H); IR (neat) 2964,2931,1786 (vs), 1263,1253 (s), 1173,1053,979,895, 876,844(vs), 753 cm-'; MS (70eV) calcd for C18H,602SSi334.1422,found 334.1420. Spiro[cyclopentane-l,l'-indan]-2,5-dione(6). To a solution of 8.7g (26mmol) of cyclobutanone 5 in benzene (100mL) was added 7.8g (28mmol) of mercuric chloride. The reaction mixture was allowed to reflux for 15 min'before being cooled and filtered

Model Compounds Related to Fredericamycin A hexane (21:4) to afford 403 mg (54%)of 1,3-dione 18 as an orange crystalline material: mp 178 "C dec; 13C NMR (CDC13) 214.36, 214.40,168.6, 167.7, 144.9, 144.6, 140.9, 137.3, 131.3, 128.2, 127.2, 126.7, 125.5, 122.0, 115.5, 71., 45.7,45.6, 31.8,22.9, 22.4, 20.5, 20.3, 20.1, 20.0 ppm; 'H NMR (CDC13)6 2.06-2.12 (m, 3 H), 2.32 (s, 3 H), 2.308 (s, 3 H), 2.312 (s, 3 H), 2.88 (br, 3 H), 3.05-3.10 (t, J = 15.0 Hz, 2 H), 3.51 (br, 2 H), 6.91 (s, 1 H), 7.05-7.10 (t,J = 15.0 Hz, 1H), 7.15-7.24 (m, 3 H); IR (KBr) 3069,2930,2854,1774 (s),1721 (s),1624, 1600,1479,1435,1372(m), 1195 (vs), 1182 (vs), 1087,1017,892,751cm-'; MS (70 eV) calcd for CnHa08 476.1471, found 476.1465. 5,6,8-Triacetoxyspiro[ 2H-benz[f]indene-2,1'-indan1-1,3dione (19). To a solution of 214 mg (0.45 mmol) of 1,3-dione 18 in acetic acid (10 mL) was added 52 pL (1.00 mmol) of bromine over a 5-min period. The reaction mixture was stirred at room temperature overnight. The reaction mixture was poured into a beaker containing water (25 mL) and was extracted with methylene chloride (3 X 25 mL). The combined organic extracts were washed with a saturated sodium sulfate solution (1X 40 mL), a saturated sodium bicarbonate solution (1 X 40 mL), water (2 X 40 mL), and a saturated sodium chloride solution 1 X 40 mL) and dried (MgS04). Concentration and recrystallization from hexane and ethyl acetate afforded 160 mg (75%) of spiro dione 19 as a yellow crystalline material: mp 105-110 "C dec; 13C NMR (CDC13) 201.6, 201.4, 168.4, 167.7, 167.3, 154.6, 154.3,146.9, 145.7, 140.6,137.4, 128.4,127.2, 126.9, 125.23, 125.19,122.9, 122.8, 122.4, 118.7,116.5,64.0,32.0,21.7,20.7,20.5,20.3 ppm; 'H NMR (CDC13) 6 2.26 (9, 3 H), 2.35 (9, 3 H), 2.51 (9, 3 H), 2.41-2.45 (t,J = 12.0 Hz, 2 H), 3.20-3.24 (t, J = 11.9 Hz, 2 H), 6.57-6.65 (d, 1 H), 6.75-6.73 (d, 1H), 7.00-7.11 (m, 2 H), 7.20-7.24 (t,J = 12.0 Hz, 1H), 7.29-7.35 (t, J = 18.1 Hz, 1H), 8.62-8.70 (d, 1 H); IR (KBr) 3070, 3024, 2938, 2858, 1776 (s), 1746, 1701 (s), 1630, 1479, 1461, 1372, 1245, 1210 (s),1193 (s), 1176 (vs), 1138, 1016, 937, 887, 750 cm-'; MS (70 eV) calcd for C2,H2,0B 472.1158, found 472.1163. I,g-Dihydroxyspiro[ 2H-benz[f]indene-2,1'-indan]-1,3dione (23). To a solution of 100 mg (0.63 mmol) of 3-cyano-l(3H)-isoben~ofuranone'~ (22)in THF (5 mL) at -78 "C was added 1.11mL (1.89 mmol, 3 equiv) of a 1.7 M tert-butyllithium solution dropwise over a 5-min period. The reaction mixture was stirred at -78 "C for 10 min, in which time the solution turned a dark red color. To the mixture was added a solution of 125 mg (0.63 mmol) of dione 2 in T H F (5 mL). The solution turned from a dark red to a very dark green color upon complete addition of the dienophile. The reaction mixture was allowed to warm to room temperature and was stirred for 3 h before being quenched with water (10 mL) and acidified to pH -1 with concentrated hydrochloric acid. The phases were separated, and the aqueous layer was extracted with ethyl acetate (3 X 30 mL). The combined organic extracts were washed with a saturated sodium chloride solution (1 X 50 mL) and dried (MgS04). Concentration and purification by flash chromatography on silica gel using hexane and ethyl acetate (3:l) afforded, after recrystallization (chloroform-hexane), 167 mg (80%) of dihydroxy spiro dione 23 as a white crystalline material: mp 246-248 "c dec; l3C (DMSO-d,) 200.8, 146.6, 145.1, 142.5, 130.0, 129.7,127.9, 126.7, 124.8, 124.3, 122.9,115.8,67.9,32.0,31.5 ppm; 'H NMR (acetone-d6)6 2.57-2.62 (t, J = 7.3 Hz, 2 H), 3.25-3.30 (t, J = 7.3 Hz, 2 H), 6.87-6.90 (d, 1 H), 7.08-7.13 (t,J = 7.3 Hz, 1 H), 7.24-7.28 (t, J = 7.3 Hz, 1 H), 7.37-7.40 (d, 1H), 7.88-7.91 (m, 2 H), 8.47-8.50 (m, 2 H), 9.52 (br, 2 H); IR (KBr) 3369, 3067, 2923, 2850, 1708 (s), 1680 (vs), 1615,1477,1473,1351,1319(s), 1261,1254,1222,1204,1029,1013 (s), 920, 777, 748, 662, 654 cm-'; MS (70 eV) calcd for C21H1404 330.0892, found 330.0888. 1,l-Diethoxyphthalan(26). 1,l-Diethoxyphthalan (26)was prepared in a 49% yield by the procedure of Contreras and MacLean.'& Physical data: bp 65 "C (0.2 mm) [lit.12*bp 60 "C (0.1 mm)]; '% NMR (CDCld 139.9,129.5,127.7,123.7,122.5,121.0, 70.5, 58.6, 15.0 ppm; 'H NMR (CDCl,) 6 1.17-1.22 (t,J = 7.2 Hz, 6 H), 3.35-3.46 (m, 2 H), 3.59-3.70 (m, 2 H), 5.09 (s, 2 H), 7.24-7.26 (d, 1H), 7.33-7.46 (m, 3 H); IR (neat) 2977,2932,2898,2871,1463, 1364,1358,1300(s), 1262 (vs), 1128 (vs), 1050,1032,1015(s), 968 (s), 763 cm-'; MS (70 eV) calcd for Cl2Hl6O3209.1178 (M + l), found 209.1181. 4-Ethoxy-4,9-epoxy-3a,9a-dihydro-9-( trimethylsily1)spiro[2H-benz[f]indene-2,1'-indan]-1,3-dione(28). By use of a modification of a procedure of Rickborn," a solution of 210 mg

J . Org. Chem., Vol. 53, No. 23,1988 5525 (1.01 mmol) of 1,l-diethoxyphthalan (26)and 17 pL (0.12 mmol) of diisopropylaminein THF (5 mL) at 0 "C was added to 317 pL (2.50 mmol) of n-butyllithium solution. The reaction mixture was allowed to stir at 0 "C for 1h to ensure complete anion generation. A solution of chlorotrimethylsilane (317 pL, 2.50 mmol) in THF (2 mL) was added dropwise to the reaction mixture. After complete addition of the chlorotrimethylsilanesolution, 200 mg (1.01 (2)in tetmmol) of spiro[3-cyclopentene-l,l'-indan]-2,5-dione rahydrofuran (5 mL) was added dropwise over a 5-min period. The ice bath was removed, and the mixture was stirred for 24 h at room temperature. To the reaction mixture was added ether (100 mL), and the resulting organic phase was washed with pH 7 buffer (3 X 40 mL) and dried (MgSO,). Concentration and purification by flash chromatography on silica gel using 20% ether in hexane with 1%triethylamine afforded 80 mg (68%) of epoxy dione 28 as a viscous clear oil: 13C NMR (acetone-d,) 204.1,201.8, 147.3, 145.2, 142.8, 140.3, 131.2,130.5, 128.9, 126.3, 125.9, 125.2, 122.4, 121.3, 73.5,69.3,63.5,58.5,53.7,45.3, 31.9, 31.4, 15.1, 0.621 ppm; 'H NMR (acetone-d,) 6 0.37 (s, 9 H), 1.18-1.23 (t,J = 7.2 Hz, 3 H), 2.53-2.58 (t,J = 7.3 Hz, 2 H), 3.23-3.28 (t,J = 7.3 Hz, 2 H), 3.32-3.39 (m, 3 H), 3.48-3.53 (d, 1 H), 6.89-6.92 (d, 1 H), 7.09-7.14 (t, J = 7.3 Hz, 1 H), 7.22-7.27 (t, J = 7.3 Hz, 1 H), 7.39-8.16 (m, 5 H); IR (neat) 3032,2940,2904,2845,1714(s),1710 (s), 1685 (vs), 1616,1474,1452,1355,1320,1270,1254,1219,1270, 1178,1036,1015(s), 991,869 (s), 777,754 em-'; MS (70 eV) calcd for C2,HZ8Sio4432.1749, found 432.1743. 4-Ethoxy-9-[ (trimethylsilyl)oxy]spiro[2H-benz[f]indene-t,l'-indan]-l,3-dione(29). To a stirred solution of 50 mg (0.12 mmol) of epoxy dione 28 in methylene chloride (2 mL) at room temperature was added 1pL (0.01 "01) of trifluoroacetic acid. The reaction mixture was stirred for 30 min and concentrated. The resulting product was purified by recrystallization from ethyl acetate and hexane to afford 49 mg (94%) of (trimethylsily1)oxy dione 29: mp 198-200 "C;13C NMR (acetone-d,) 203.7, 202.3, 154.3, 151.6, 146.3, 146.1, 132.5, 131.7, 130.6, 129.5, 129.1, 128.7, 126.3, 125.7, 125.2, 122.3, 121.6,120.3,65.4,58.2,31.7, 31.2, 15.3, 0.669 ppm; 'H NMR (acetone-cl,) 6 0.35 (s, 9 H), 1.16-1.21 (t, J = 7.2 Hz, 3 H), 2.55-2.59 (t, J = 6.9 Hz, 2 H), 3.40-3.47 (m, 4 H), 6.85-6.88 (d, 1 H), 7.16-7.21 (t,J = 7.3 Hz, 1H), 7.23-7.28 (t,J = 7.3 Hz, 1H), 7.59-7.78 (m, 5 H), 7.95-7.80 (t,J = 7.3 Hz, 1H), 8.34-8.37 (d, 1H); IR (KBr) 3058, 2995, 2956, 2851, 1708 (s), 1700 (s), 1682 (vs), 1614, 1593, 1476, 1445, 1340, 1270,1249,1173,1076,1023 (s), 1001,969,776,748cm-'; MS (70 e v ) calcd for C26H26Si04 430.1593, found 430.1595. 2-Hydroxy-4-methoxybenzoic Acid (30). To a solution of 15.4 g (0.1 mol) of 2,4-dihydroxybenzoic acid in 20% sodium hydroxide (50 mL) was added 10.41 mL (0.11 mol) of dimethyl sulfate. The resulting orange solution was stirred at room temperature for 2 h before being neutralized with concentrated hydrochloric acid. The mixture was extracted with ether (3 X 100 mL), and the aqueous layer was acidified to pH -1 with concentrated hydrochloric acid, resulting in a white precipitate. The precipitate was collected by filtration, washed with water, and dried (under vacuum). Recrystallizationfrom ethanol and water afforded 9.2 g (56%) of acid 30 as a white flocculant material: mp 157-159 "C; 13C NMR (acetone-cl,) 172.5, 166.9,165.3, 132.6, 107.9, 106.0, 101.5, 55.9 ppm; 'H NMR (acetone-d,) 6 3.84 (s, 3 H), 6.44-6.51 (m, 2 H), 7.77-7.80 (d, 1H), 11.38 (br, 2 H); IR (KBr) 3050-2554 (br), 1649 (vs), 1624 (vs), 1576, 1504, 1465, 1436 (s), 1384, 1360, 1316, 1261 (vs), 1250 (s), 1226, 1205, 1176, 1150 (s), 1095, 1025,966,962, 841, 780, 715,693,627 cm-'. 2,5-Dihydroxy-4-methoxybenzoic Acid (31). To a solution of 10.0 g (59.5 mmol) of acid 30 and 8.0 g (200 mmol) of sodium hydroxide in water (90 mL) at 10 "C was added a solution of 17.0 g (62.9 mmol) of potassium persulfate in water (300 mL) over a 4-h period. The reaction mixture was then allowed to warm to room temperature and was stirred for 24 h, resulting in a purple solution. The mixture was neutralized with 1N hydrochloric acid and extracted with ether (3 X 150 mL). The combined organic extracts were washed with a saturated sodium chloride solution (1 X 250 mL) and dried (MgS04). Concentration and recrystallization from ethanol and water afforded 3.21 g (30%) of acid 31 as colorless rectangular rods: mp 200-202 "C dec (lit.23mp (23) Rajagopalan, S.; Seshadri, T. R.; Varadarajan, S. Proc. Indian Acad. Sei. 1949, 30A, 265.

5526 J . Org. Chem., Vol. 53, No. 23, 1988 201 "C dec); 13CNMR (DMSO-&) 172.0, 159.5, 157.6, 135.2, 121.8, 103.6, 100.1,55.8 ppm; 'H NMR (DMSO-d,) 6 3.79 (9, 3 H), 6.50 (9, 1 H), 7.84 (9, 1 H), 11.30 (br, 1 H); IR (KBr) 3599, 3543, 3400-2553 (br), 1680 (s), 1626 (s), 1507, 1424, 1272 (s), 1239 (s), 1219 (vs), 1193 (vs), 1159 (s), 1050 (vs), 881, 801, 705, 606 cm-'. 2,4,5-TrimethoxybenzoicAcid (32). To 3.00 g (16.3 mmol) of acid 31 and 7.2 g (52 mmol) of potassium carbonate in refluxing acetone (100 mL) was added 4.6 mL (49 mmol) of dimethyl sulfate. The reaction mixture was stirred for 3 h a t 56 OC and was then filtered through a coarse sintered-glass funnel containing Celite (washingwith acetone) to remove the inorganic salts. The organic phase was washed with a saturated sodium chloride solution (1 X 100 mL) and dried (MgSO,). Concentration gave a brown solid (methyl 2,4,54rimethoxybenzoate) suitable for use in the next step. The crude product was added to a 5% potassium hydroxide solution (100 mL) and refluxed for 0.5 h. After cooling, the saponified mixture was acidified to pH 1 with concentrated hydrochloric acid and extracted with ethyl acetate (3 X 50 mL). The combined organic extracts were washed with a saturated sodium chloride solution (1 X 100 mL) and dried (MgSO,). Concentration and recrystallization from benzene and petroleum ether afforded 2.61 g (76%) of 2,4,5-trimethoxybenzoic acid (32) as colorless needles: mp 143-144 OC (Aldrich Chemical Co., mp 144-145 "C); I3C NMR (CDCl3) 165.3, 154.4, 153.7, 144.0, 114.5, 109.0, 96.3, 57.2, 56.2 ppm; 'H NMR (CDC13)6 3.89 (s, 3 H), 3.98 (9, 3 H), 4.08 (9, 3 H), 6.59 (9, 1 H), 7.59 (s, 1 H), 10.73 (s, 1 H); IR (KBr) 3246 (br), 2967, 2963, 2835, 1710 (vs), 1614 (s), 1521, 1480,1443,1419,1361,1287,1268,1235,1213 (s), 1170,1044,1021 (s), 876, 841, 826 cm-'; MS (70 eV) calcd for C10H12O5 212.0684, found 212.0681. N,N-Diethyl-2,4,5-trimethoxybenzamide (33). To a solution of 2,4,5-trimethoxybenzoicacid (32)in benzene of 10.0 g (47 "01) (100 mL) a t room temperature was added 8.6 mL (118 mmol) of thionyl chloride dropwise over a 10-min period. The reaction mixture was stirred at room temperature for 2 h, while vigorous gas evolution occurred. The green solution was then stirred overnight, resulting in a yellow solution. The solvent and excess thionyl chloride were removed by distillation, resulting in a white solid. The solid was dried under vacuum to afford 10.6 g (98%) of crude 2,4,5-trimethoxybenzoyl chloride suitable for use in the next step. To a solution of the crude benzoyl chloride in benzene (100 mL) a t 0 "C was added 9.8 mL (94 mmol) of diethylamine dropwise over a 10-min period. The evolution of gas was observed as the benzoyl chloride dissolved, resulting in a viscous orangebrown colored solution. The cooling bath was removed and the reaction mixture was allowed to stir at room temperature for 12 h. The mixture was washed with 1 N hydrochloric acid (2 X 100 mL) and a saturated sodium chloride solution (1 X 100 mL) and dried (MgSO,). Concentration and recrystallization from ethyl acetate and hexane afforded 11.64 g (93%) of benzamide 33 as a white crystalline material: mp 74-75 "C (litzmmp 73-74 "C); 13C NMR (CDCI,) 168.3, 150.0, 149.5, 143.2, 118.2, 111.3, 97.7, 56.45, 56.36, 55.9, 42.7, 38.7, 13.8, 12.7 ppm; 'H NMR (CDCl,) 6 1.03-1.08 (t, J = 7.1 Hz, 3 H), 1.23-1.26 (t, J = 7.1 Hz, 3 H), 3.16-3.23 (9, J = 7.1 Hz, 2 H), 3.56 (br, 2 H), 3.80 (s, 3 H), 3.84 (s, 3 H), 3.90 (s, 3 H), 6.53 (9, 1 H), 6.76 (s, 1 H); IR (KBr) 2971, 2935, 2848, 1626 (vs), 1216 (s), 1517 (s), 1475 (s), 1456, 1428, 1390, 1335, 1283 (s), 1212 (vs), 1151, 1037, 1023 (s), 857,831,807 cm-'; MS (70 eV) calcd for C14H21N04267.1471, found 267.1464. 3-Hydroxy-4,5,7-trimethoxy-1(3H)-isobenzofui.anone (34). By use of a modification of a reported procedure,24a mixture of 5.00 g (19 mmol) of N,N-diethyl-2,4,5-trimethoxybenzamide (33) and 3.2 mL (21 mmol) of N,N,NN/,N'-tetramethylethylenediamine in THF (30 mL) were cooled to -78 "C. To the solution was added 16.2 mL (21 mmol, 1.3 M) of a see-butyllithium solution dropwise with vigorous stirring over a 20-min period, followed by 45 min of stirring a t -78 OC. To the cloudy yellow solution was added 6.4 mL (83 mmol) of N,N-dimethylformamide via syringe. The solution was stirred for 30 min at -78 "C, warmed to room temperature, and stirred for 18 h. Water (50 mL) was added, and

-

(24) Chenard, B. L.; Dolson, M. G.; Sercel,A. D.; Swenton,J. S. J. Org. Chem. 1984,49, 318.

Evans et al. the solution was acidified to pH -1 with concentrated hydrochloric acid. The phases were separated, and the aqueous layer was extracted with ethyl acetate (3 X 60 mL). The combined organic layers were concentrated at reduced pressure to give a yellow residue. The residue was dissolved in methylene chloride (100 mL) and washed with 1 N hydrochloric acid (1 X 50 mL) and water (1 X 50 mL) and dried over calcium sulfate. Concentration afforded N,N-diethyl-6-formyl-2,4,5-trimethoxybenzamide as an orange oil suitable for use in the next step. The oil was dissolved in acetic acid (100 mL),and 10% aqueous hydrochloric acid (100 mL) was added. The mixture was refluxed for 18 h, and the solvent was removed in vacuo at 40-45 "C. The resulting red residue was taken up in ethyl acetate (100 mL), and water was added (100 mL). The mixture was filtered, and the solid product was saved. The phases were separated, and the aqueous layer was extracted with ethyl acetate (2 X 75 mL). The organic layer was extracted with saturated aqueous sodium bicarbonate solution (3 X 50 mL). The basic layer was cooled to 0 "C, carefully acidified to pH -1 with concentrated hydrochloric This combined acid, and extracted with ethyl acetate (3 X 75 A). organic layer was washed with a saturated sodium chloride solution (1 X 125 mL) and dried (MgSO,). Concentration resulted in a red-brown solid, which was combined with the filtered product and recrystallized (ethyl acetate-hexane) to afford 215 mg (47%) (34)as a of 3-hydroxy-4,5,7-trimethoxy-l(3H)-isobenzofuranone white crystalline material: mp 215-216 "C; 13CNMR (DMs0-d~) 165.4, 158.8, 154.6, 140.3, 137.2, 105.1, 99.2, 94.6, 60.5, 56.6, 56.2 ppm; 'H NMR (acetone-d,) 6 3.83 (9, 3 H), 3.93 (9, 3 H), 3.99 (s, 3 H), 6.54-6.57 (d, 1H), 6.73-6.75 (d, 1H), 6.84 (s, 1H); IR (KBr) 3462 (s),3095,3014,2997,2953,2845,1774 (vs), 1625,1608,1516 (s), 1448, 1438, 1360, 1340, 1321, 1248, 1238 (s), 1121, 1153, 1053 (s), 1016, 970,953, 938, 853,841,770 cm-'; MS (70 eV) calcd for CllHlzO6 240.0634, found 240.0630. 3-Cyan0-4,5,7-trimethoxy-l(3H)-isobenzofuranone (35). To a mixture of 550 mg (2.3 mmol) of 3-hydroxy-4,5,7-trimethoxy-1(3H)-isobenzofuranone (34)and 1.79 g (27.5 mmol, 12 equiv) of potassium cyanide was added water (30 mL). The solution was stirred in a well-vented hood at room temperature for 15 min to effect a homogeneous clear orange solution, and concentrated hydrochloric acid (20 mL) was added. The mixture was allowed to stand at room temperature for 3 h, and the resulting precipitated product was filtered and vacuum-dried to afford 533 mg (93%) of 3-cyano-4,5,7-trimethoxy-1(3H)-isobenzofuranone (35) as a tan crystalline material: mp 129-130 OC; NMR (acetoned,) 165.2, 160.7, 157.0, 137.3, 136.9, 115.6, 103.9, 100.5, 63.5, 60.9, 57.2, 56.9 ppm; 'H NMR (acetone-d,) 6 3.91 (5, 3 H), 3.9 (s, 3 H), 4.07 (9, 3 H), 6.40 (9, 1 H), 6.94 (aromatic, s, 1 H); IR (KBr) 3088,2990,2950,2938,2856,1788 (s),1776 (vs), 1629,1609,1515 (s), 1473, 1440, 1357 (s), 1338, 1272, 1247, 1234 (s), 1207 (s), 1182, 1152, 1091, 1067, 1028 (vs), 1017 (vs), 1003 (s),964, 840,762 cm-'; MS (70 eV) calcd for C12HllN05249.0637, found 249.0640. Anal. Calcd for ClzHl1NO5: C, 57.83; H, 4.45; N, 5.62. Found: C, 57.67; H, 4.35; N, 5.46. 4,9-Dihydroxy-5,6,8-trimethoxyspiro[2H-benz[ flindene2,1N/-indan]-1,3-dione (38). To a solution of 50 mg (0.20 mmol) (35)in THF of 3-cyano-4,5,7-trimethoxy-1(3H)-isobenzofuranone (2mL) a t -78 "C was added 130 FL (0.20 mmol) of an n-butyllithium solution over a 2-min period. The mixture was stirred a t -78 "C for 30 min before 31 fiL (0.24 mmol, 1.2 equiv) of chlorotrimethylsilane was added. The orange reaction mixture was then warmed to room temperature and stirred for 5 min, before cooled to -78 "C. To the mixture a t -78 "C was added a solution of 44 mg (0.22 mmol, 1.1equiv) of spiro dione 2 in THF (2 mL) over a 5-min period. The resulting red solution was warmed to room temperature (resulting in a green solution) and stirred for 6 h. To the reaction mixture was added ethyl acetate (10 mL) and water (10 mL), and the phases were separated. The organic phase was washed with water (2 X 5 mL) and a saturated sodium chloride solution (1X 5 mL) and dried (MgSOJ. 'H NMFt (CDCI,) of the crude reaction mixture indicated the presence of a (trimethylsily1)oxyresonance at 0.18 ppm, which was lost upon chromatographic workup. Purification by flash chromatography on silica gel using hexane and ethyl acetate (2:l) afforded 5 1 mg (62%) of 38 as a tan crystalline material: mp 168-171 "C dec; 13CNMR (CDC1,) 201.2, 201.1, 154.6, 153.2, 146.5, 145.2, 143.0, 140.6, 131.2, 130.3, 129.8, 127.9, 126.3, 124.8, 124.4, 119.3, 118.9,

J. Org. Chem. 1988, 53, 5527-5530 116.2, 65.7, 57.1, 56.4, 56.3, 31.8, 32.9 ppm; 'H NMR (CDC13)6 2.56-2.61 (t, J = 7.2 Hz, 2 H), 3.27-3.22 (t, J = 7.2 Hz, 2 H), 4.01 (s, 3 H), 4.07 (9, 3 H), 4.10 (9, 3 H), 6.78-6.80 (s, 1 H), 6.87 (9, 1 H), 7.04-7.09 (t, 1H), 7.18-7.23 (t,1H), 7.31-7.33 (d, 1H), 10.40 (br, 1 H), 10.70 (s, 1 H); IR (KBr) 3372, 3069, 3020, 2925, 2856, 1709 (s), 1688 (vs), 1620, 1478, 1470, 1322 (s), 1260, 1253, 1208 (s), 1197 (s), 1172 (vs), 1012 (s), 789, 749 cm-'; MS (70 eV) calcd for CZ4Hz0O7 420.1203, found 420.1199.

Acknowledgment is made to the donors of the Petroleum Research Fund, administered by the American Chemical Society, for the support of this research.

5527

Registry No. 1, 80455-68-1;2, 105365-18-2;3, 17082-61-0;4, 100020-34-6;4 (ketone), 83-33-0; 5 , 116664-59-6;6, 116664-60-9; (*)-7,116698-30-7; (*)-8,11669&31-8; (*)-9a, 116664-61-0;(*)-9b, 116698-32-9; (&)-lo (isomer l ) , 116664-62-1; (*)-lo (isomer 2), 116698-33-0; 14, 526-86-3; 15, 5091-02-1; 16, 116664-63-2; 18, 116664-64-3; (&)-19, 116664-65-4; (*)-22, 116664-66-5; 23, 116664-67-6; 26, 70103-17-2; (*)-28, 116664-68-7; (f)-29, 116669-32-0;30, 2237-36-7; 31, 5981-37-3; 32, 490-64-2; 32 (Me ester), 20029-76-9;32 (acid chloride), 42833-66-9; 33,105518-12-5; 33 (6-formyl deriv), 105518-13-6; (*)-34, 116664-69-8; (*)-35, 116664-70-1;(*)-38,116664-71-2; CHz=CHCH=CH,, 106-99-0; 2,4-(OH)zC6H3COzH, 89-86-1; Danishefsky diene, 54125-02-9.

Acid-Catalyzed Transformations of the Heliangolide 15-Hydroxya~etylleptocarpin~ Laura Alvarezt and Guillermo Delgado* Instituto de Qulmica de la Universidad Nacional AutBnoma de Mgxico, Circuit0 Exterior, Ciudad Universitaria, Coyoacdn 04510, Mlxico, D.F., Mlxico

Received March 22, 1988 Acid-catalyzed transformations of the natural heliangolide 15-hydroxyacetylleptocarpin (1)were carried out. Treatment with perchloric acid afforded three elimination products: the dihydropyran 2, its acetate 3, and the germacratrienolide 4. BF3.0Et, catalysis gave 5, derived from a pinacolic rearrangement followed of hemiketalization. In all these cases, the l(lO)-epoxidepromoted the reaction. Allylic chloride 7 was obtained by treatment of 1 with S0Clz. Mechanisms explaining the characterized reaction products are proposed. The acid-catalyzed cyclizations of germacrolides (trans,trans-1(10),4-germacradienolides) and their l(10)and 4(5)-epoxy derivatives have been extensively investigated because of interest in their biogenesis, the reaction mechanisms and the transformation products.2 The reactions afford eudesmanolides, elemanolides, and guaianolides as the main products, and in some cases xanthanolides3v4and cyclobutane derivatives3 have also been obtained. Few acid-catalyzed studies have been performed on the geometric isomers of germacranolide~.~ However, cyclization of heliangolides (l(lO)-trans,4-cis-germacradienolides) gave ~adinanolides~~' and eude~manolides~ in low yield.8 The present work deals with the acid-catalyzed transformations of the heliangolide 15-hydroxyacetylleptocarpin (1),9which yields products of functional groups transformations and rearrangements. The expected cadinanolides or eudesmanolides were not observed. Treatment of 1 with perchloric acid in acetone for 1 h gave a mixture of compounds 2-4. The major product 2, mp 162-164 "C, analyzed for C20H2406(elemental analysis and mass spectrometry), indicated that a C2H402unit was lost in the reaction. Its IR spectrum showed the presence of hydroxyl (3540 cm-'), an a,@-unsaturatedy-lactone (1755 cm-l), and double bonds (1640 cm-l). 'H NMR spin decoupling experiments established the sequence from H-5 to H-9and corresponded to the arrangement in 1. Major differences are (a) the one-proton multiplet at 6 5.85, (b) an additional AB system of an oxymethylene group, overlapping with the AB system corresponding to H-15, and (c) the (2-10 methyl resonance of the starting material was no longer present. The 13C NMR spectrum of 2 confirmed the presence of an additional trisubstituted double 'Present address: Departamento de Quimica OrgHnLca, DivisiBn de Estudios Superiores de la Facultad de Ciencias Quimicas e Industriales, Universidad AutBnoma del Estado de Morelos, Avenida Universidad 1001, Cuernavaca, Morelos, MBxico.

bond and the additional oxymethylene group, indicating the presence of a A3-dihydropyran ring. Addition of trichloroacetyl isocyanate (TAI) induced a paramagnetic shift (A6 1.16) in the signal of H-15, thus establishing that C-15 beared the primary allylic alcohol.1° These data led to the deduction of structure 2. X-ray crystallographic analysis of 2 confirmed the proposed structure. Figure 1 is a computer-perspective drawing of the solved structure, and details of this analysis are included in the Experimental Section. The tricyclic structure 2 adopts the chair-boat conformation representative of the heliangolides," although it is qualitatively more rigid than that of the starting material. (1)Contribution No. 903 from the Instituto de Quimica de la Universidad Nacional Autdnoma de Mgxico. This work was presented in part at the Third Chemical Congress of North America, Toronto, Ontario, Canada, June 1988. (2)For an account of cyclization studies of germacranolides see: Fischer, N. H.; Olivier, E. J.; Fischer, H. D. h o g . Chem. Org. Nat. Prod. 1979,38, 105-110. (3)Wilton, J. H.; Doskotch, R. W. J. Org. Chem. 1983,48,4251. Chem. Commun. 1986, (4)Parodi, F. J.; Fischer, N. H. J. Chem. SOC., 1405. (5) Cyclization of simple germacra-I,&dienes have been performed, inter alia, see: (a) Sutherland, J. K. Tetrahedron 1974,30, 1651. (b) Takeda, K. Tetrahedron 1974,30,1525.(c) Kodama, M.;Yokoo, Sh.; Matsuki, Y.; Ito, Sh. Tetrahedron Lett. 1979,1687. (d) Williams, J. R.; Callahan, J. F.; Blount, J. F. J. Org. Chem. 1981,46,2665. (6)Toma, K.;Murae, T.; Takahashi, T. Chem. Lett. 1982,551. (7) De Pascual Teresa, J.; Gonzaez, M. S.; Caballero, M. C.; Parra, T.; Bellido, I. S. Tetrahedron Lett. 1987,821. (8)A photocyclization of an heliangolide has been reported: Chowdhury, P. K.; Sharma, R. P.; Baruah, J. N. Tetrahedron Lett. 1983,5429. (9)PBrez, A.; C o h , M.; Guerrero, C.; Cruz, M. L.; Romo de Vivar, A. Phytochemistry 1984,23,823. (10)Samek, Z.;Budesinsky,M.; Collect. Czech, Chem. Commun. 1979, 44,558. (11)Herz, W.; de Groote, R.; Murari, R.; Blount, J. F. J. Org. Chem. 1978,43,3559. Nishikawa, M.;Kamiya, K.; Takabataki, A.; Oshio, H.; Tomie, Y.; Nitta, I. Tetrahedron 1966,22,3601.McPhail, A.T.; Onan, K. D. J. Chem. SOC., Perkin Trans. 1976, 2, 578. Doskotch, R. W.; Fairchaild, E. H.; Huang, Ch. T.; Wilton, J. H.; Beno, M. A.; Cristoph, G. G. J. Org. Chem. 1980,45,1441.

0022-326318811953-5527$01.50/0 0 1988 American Chemical Society