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Antibiotic Pollution in Marine Food Webs in Laizhou Bay, North China: Trophodynamics and Human Exposure Implication Sisi Liu, Hongxia Zhao, Hans-Joachim Lehmler, Xiyun Cai, and Jingwen Chen Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.6b04556 • Publication Date (Web): 20 Jan 2017 Downloaded from http://pubs.acs.org on January 21, 2017

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Environmental Science & Technology

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Antibiotic Pollution in Marine Food Webs in Laizhou Bay, North

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China: Trophodynamics and Human Exposure Implication

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Sisi Liu,† Hongxia Zhao,*† Hans-Joachim Lehmler,§ Xiyun Cai,† Jingwen Chen*†

4 5 6

†

7

Education), School of Environmental Science and Technology, Dalian University of

8

Technology, Linggong Road 2, Dalian 116024, China

9

§

10

Key Laboratory of Industrial Ecology and Environmental Engineering (Ministry of

Department of Occupational and Environmental Health, College of Public Health,

The University of Iowa, IA 52242, USA

11

1

ACS Paragon Plus Environment


12

2


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ABSTRACT

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Little information is available about the bioaccumulation and biomagnification of

15

antibiotics in marine food webs. Here we investigate the levels and trophic transfer of

16

9 sulfonamide (SA), 5 fluoroquinolone (FQ), and 4 macrolide (ML) antibiotics, as

17

well as trimethoprim in nine invertebrate and ten fish species collected from a marine

18

food web in Laizhou Bay, North China in 2014 and 2015. All the antibiotics were

19

detected in the marine organisms, with SAs and FQs being the most abundant

20

antibiotics. Benthic fish accumulated more SAs than invertebrates and pelagic fish,

21

while invertebrates exhibited higher FQ levels than fish. Generally, SAs and

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trimethoprim biomagnified in the food web, while the FQs and MLs were biodiluted.

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Trophic magnification factors (TMF) were 1.2 - 3.9 for SAs and trimethoprim, 0.3 -

24

1.0 for FQs and MLs. Limited biotransformation and relatively high assimilation

25

efficiencies are the likely reasons for the biomagnification of SAs. The pH dependent

26

distribution coefficients (logD) but not the lipophilicity (logKOW) of SAs and FQs had

27

a significant correlation (r = 0.73; p < 0.05) with their TMFs. Although the calculated

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estimated daily intakes (EDI) for antibiotics suggest that consumption of seafood from

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Laizhou Bay is not associated with significant human health risks, this study provides

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important insights into the guidance of risk management of antibiotics.

31 32

Keywords: Antibiotics, Biomagnification, Health risk, Trophic dilution

3



33

INTRODUCTION

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Antibiotics are largely used in human medicine, animal husbandry, agriculture and

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aquaculture. In 2013 alone, China, the largest producer and user of antibiotics in the

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world, used about 162,000 tons of antibiotics.1,2 Most antibiotics are only partially

37

metabolized in humans or animals.3 Approximately 60% of antibiotics are ultimately

38

released as the parent compounds into the environment.2 As a consequence, over 30

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antibiotics, including sulfonamide (SA), fluoroquinolone (FQ), and macrolide (ML)

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antibiotics, have been detected with concentrations up to µg/L levels in surface waters

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and ng/g levels in sediments.4-7 For example, eleven antibiotics were found in

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seawater of Bohai Bay, North China with the total concentrations ranging from 2.3 to

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6,800 ng/L.4 Seventeen antibiotics were detected in sediments from East Sea of China

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at concentrations ranging from not detected to 537.4 ng/g dry weight (dw).5

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Continuous inputs from numerous pollution sources have led to antibiotics with the

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characteristic of “pseudo-persistence” (i.e., the inputs of antibiotics exceed the ability

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of the ecosystem to remove them completely from the natural environment8) in

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natural water environment, which increases the possibility of bioaccumulation and

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biomagnification of antibiotics in aquatic organisms. Furthermore, antibiotics can give

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rise to an increase in the antibiotic resistance of pathogenic bacteria, which is a

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particular concern.1 As a result, there is a need to investigate and assess the negative

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effects of antibiotic contamination on ecosystem and, ultimately, human health.

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Antibiotics have been reported to have the potential to accumulate in aquatic biota

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by several field-monitoring data in aquatic organisms at various trophic levels, such as

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aquatic plants, mussels, and fish.7,9-11 For example, the concentrations of ΣSAs, ΣFQs

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and ΣMLs were as high as 15 ng/g dw, 6,500 ng/g dw, and 6 ng/g dw, respectively, in

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hydrophytes from Baiyangdian Lake, North China,7 and 77 ng/g dw, 1,600 ng/g dw, 4


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and 36 ng/g dw, respectively, in mollusks from Bohai Sea, China.9 Higher

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concentrations of ΣSAs and ΣMLs were found in wild fish from the Haihe River,

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North China and reached to 996 ng/g dw and 45 ng/g dw, respectively.10 Consistent

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with the occurrence of antibiotics in wild aquatic species, our earlier laboratory study

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revealed significant bioconcentration of sulfadiazine and sulfamethoxazole (BCF

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values: 1.7 - 170 L/kg) in common carp (Cyprinus carpio) at environmentally relevant

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concentrations.12 Hence, these antibiotics (i.e., SAs, FQs and MLs) may have a

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comparable trophic magnification potential and, thus, pose a risk for aquatic

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organisms, and even humans. Trophodynamics of these chemicals in aquatic food

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webs are an important criterion for assessing their ecological and health risks.13

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However, there are only limited works about the trophic transfer of antibiotics in

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aquatic food webs.14,15 Boonsaner et al. found that oxytetracycline can transfer into

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seven-striped carp (Probarbus jullieni) from watermeal (Wolffia globosa).14

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Roxithromycin was reported to have significant bioaccumulation through dietary

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uptake but no biomagnification potential in an artificial food chain including algae,

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water flea and fish.15 These studies only included one antibiotic, and the artificial food

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chains investigated consisted of two or three species. Overall, little comprehensive

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data are available about the trophodynamics of antibiotics to aquatic organisms, in

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particular with respect to marine food webs.

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To explore the trophodynamics of antibiotics in aquatic ecosystems, this study

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analyzed 19 widely-used antibiotics (Table S1), including 9 SA, 5 FQ, and 4 ML

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antibiotics, as well as trimethoprim, in a marine food web (9 invertebrate and 10 fish

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species) from the coastal area of Laizhou Bay, North China. The trophic levels of all

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organisms were determined by using the stable carbon and nitrogen isotope method.

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The influence of physicochemical factors including octanol/water partition coefficient 5



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(KOW), fraction of neutral molecules (fn) and pH dependent distribution coefficient

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(logD) on trophodynamics of antibiotics was also investigated. In addition, antibiotic

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concentrations were used to assess human health risks associated with the

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consumption of seafood from Laizhou Bay. This study presents the first

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characterization of trophic transfer of antibiotics in the marine food web of Laizhou

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Bay, and provides important insights that can guide the risk assessment of antibiotics.

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EXPERIMENTAL SECTION

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Sampling. Samples of marine organism, including 9 invertebrate species (3

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shellfish, 5 crustaceans, and 1 cephalopod) and 10 fish species (Table S2), were

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collected from Laizhou Bay with a bottom trawl from March 2014 to April 2015. The

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sampling months and sites for each species are described in detail in Table S2. The

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location of sampling sites is shown in Figure S1.

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All the samples were sealed in polytetrafluoroethylene plastic bags and transported

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immediately on ice to the laboratory. The invertebrates and fish species were cleaned

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with deionized water, and their lengths and weights were measured. Because the

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reproducible homogenization of whole marine organisms is challenging, the following

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tissues were dissected: soft abdomen for crabs; soft body for other invertebrates; and

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muscle from the backbone for fish. The tissue samples from one to six similarly sized

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individuals for the same species were homogenized, lyophilized, wrapped in

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aluminum foil and stored at -20°C until analysis. Three different homogenized

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samples were analyzed for each species.

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Sample Preparation and Instrumental Analysis. Sample extraction and clean-up

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were performed following a reported method with minor modifications.7 Briefly,

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target antibiotics were extracted from the biota samples with an accelerated solvent 6


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extraction system (ASE 350; Dionex, Sunnyvale, CA, USA) and purified using Oasis

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HLB cartridges (6 mL, 500 mg; Waters, Milford, MA, USA) as detailed in the

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Supporting Information (SI).

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Chemical analysis was performed on an Agilent 1100 high performance liquid

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chromatograph-tandem 6410B quadrupole mass spectrometer (LC-MS/MS) equipped

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with an electrospray ionization (ESI) source using multiple reaction monitoring

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(MRM) positive mode. Ten µL of the final extract were injected into an XTerra® MS

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C18 column (2.1 mm × 100 mm, 3.5 µm; Waters, Milford, MA, USA) maintained at

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40 °C. A gradient elution program was initiated with 95% A (0.1% v/v formic acid

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with 15.9 mM ammonium formate in water, pH = 2.4) and 5% B

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(methanol/acetonitrile, 1/1 in v/v), followed by a linear gradient from 5% to 88% B (0

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- 30 min) at a flow rate of 0.3 mL/min. The MRM transitions and fragment voltage,

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collision energy, and retention times are listed in Table S3.

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The carbon stable-isotope ratio (δ13C) and nitrogen stable-isotope ratio (δ15N) of

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the samples were determined using an Isoprime 100 isotope ratio mass spectrometer

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interfaced to a vario PYRO cube elemental analyzer (Elementar, Hanau, Germany) as

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described in the SI.

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Quality Assurance and Quality Control. All the analytical standards had purities

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exceeding 98% and their sources are described in the SI. The method detection limits

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(MLOD) and the method quantification limits (MLOQ) are defined as three and ten

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times the standard deviation (SD) of the mean procedural blanks (n = 6), respectively.

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The MLODs and MLOQs ranged from 0.02 to 1.5 ng/g wet weight (ww) and 0.08 to

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5.0 ng/g ww, respectively (Table S4). The recoveries (n = 6) of SAs, FQs, MLs and

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trimethoprim from spiked samples (i.e., farm raised fish) were 67% to 117%, 49% to

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128%, 64% to 111% and 90%, respectively, and the relative standard deviations (RSD) 7



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were less than 19% (Table S4). All samples were spiked with surrogate standards

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prior to extraction to assess recoveries of target compounds in biotic samples (Table

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S4). The recoveries of the surrogate standards varied from 32% to 121%. The reported

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final concentrations of antibiotics were corrected for the recoveries of their respective

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surrogates.

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The levels of all the target antibiotics in solvent blanks were below or near to their

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respective MLODs. Enrofloxacin, ofloxacin, ciprofloxacin, norfloxacin and enoxacin

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were detected in 58% to 71% of the procedural blanks. Concentrations of these five

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antibiotics in procedural blanks were subtracted from those in the corresponding

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samples for batches with blank contamination.

143 144

Data Analysis. To account for lipid effects on δ13C, all δ13C values were adjusted for the C/N ratios as follows:16 δ 13 C adjusted = δ 13 C - 3.32 + 0.99 × C/N

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(1)

Trophic level of a consumer (TLconsumer) was calculated using the following equation: TLconsumer = (δ 15 N consumer - δ 15 N baseline ) / ∆N + TLbaseline

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(2)

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where ∆N is the enrichment factor of 3.8‰ representing the increase in δ15N from one

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TL to the next;17,18 TLbaseline is the trophic level of the baseline indicator. In the present

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study, northern maoxia shrimp (Acetes chinensis) was selected as the baseline

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indicator and assigned to TL = 2 according to previous studies.19,20

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The relative contribution of benthic vs. pelagic carbon sources in the diets of biota

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(i.e., relative carbon sources) was calculated using δ15N and δ13C values as detailed in

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the SI. 21

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The antibiotic concentration below the MLOQ was assigned a randomly-generated

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value between zero and the compound-specific MLOQ for statistical analysis. The 8


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random values obtained followed normal distribution. The data were log-transformed

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prior to analysis to obtain a greater homogeneity of variance. The differences in

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antibiotic concentrations between invertebrates and fish species and between benthic

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and pelagic fish were analyzed using the Student's t-test. The Mann-Whitney U test

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was used if the data were not normally distributed. All the data are presented as the

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arithmetic mean ± one standard deviation if they follow normal distribution otherwise

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the geometric mean (GM) values and 95% confidence intervals (CI) are given. A

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p-value of < 0.05 was considered statistically significant.

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RESULTS AND DISCUSSION

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Antibiotic Concentrations and Profiles in the Biotas. The detection frequencies

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of the 19 target antibiotics in all the samples are listed in Table S5. Sulfamethoxazole

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had the highest detection frequency (95%), followed by trimethoprim (91%).

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Ofloxacin (67%) and anhydroerythromycin (58%) occurred with the highest detection

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frequency among FQs and MLs, respectively. Among the marine species investigated,

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the swimming crab (Portunus trituberculatus) was the organism with the highest

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detection frequency (90%), while the fat greenling (Hexagrammos otakii) had the

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lowest detection frequency (21%) (Table S6). Overall, the high detection frequencies

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indicate widespread antibiotic contamination in marine organisms from Laizhou Bay.

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The total concentrations of all the antibiotics ranged from 57 to 1,370 ng/g ww

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(GM (95% CI): 300 (250, 360) ng/g ww) in all the biota samples. The total antibiotic

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concentrations in invertebrates (range: 57 to 1,230 ng/g ww; 370 (270, 500) ng/g ww)

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were significantly higher (p < 0.05) than those in fish (98 to 610 ng/g ww; 240 (200,

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300) ng/g ww). Benthic fish (i.e., flathead, eelgoby, javeline goby, fat greenling and

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tongue sole; 170 to 610 ng/g ww, mean ± SD: 360 ± 130 ng/g ww) tended to 9



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accumulate more antibiotics (p < 0.05) than pelagic fish (i.e., halfbeak, dotted gizzard

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shad, silvery pomfret, Chinese sea perch and mullet; 98 to 440 ng/g ww, 190 (150,

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240) ng/g ww). The highest antibiotic residues were detected in mactra quadrangularis

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(Mactra veneriformis; 1,300 ± 90 ng/g ww), with FQs accounting for 90% of the total

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antibiotic concentrations. Similar observations have been reported for mollusks

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(Meretrix merehjgntrix; 0.7 to 1,580 ng/g dw) from the Bohai Sea, China.9 The lowest

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antibiotic concentrations were detected in northern maoxia shrimp (64 ± 10 ng/g ww),

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most likely due to their variable exposure to antibiotics associated with their strong

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floating habit.17

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Concentrations of Σ SAs, ΣFQs, ΣMLs and trimethoprim in all the biota samples

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ranged from 19 to 451 ng/g ww, not detected (nd) to 1,200 ng/g ww, nd to 15 ng/g

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ww and nd to 50 ng/g ww, respectively. SAs and FQs were the most abundant

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antibiotic classes detected in most biota samples, accounting for 52% and 45% of total

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antibiotic concentrations, respectively (Figure 1). Trimethoprim (2%) and MLs (1%)

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made only minor contributions to the total antibiotic concentrations. The levels of

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ΣFQs in invertebrates (175 (92, 330) ng/g ww) were significantly higher (p < 0.05)

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than those in fish (56 (38, 81) ng/g ww). The opposite trend was found for ΣSAs

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(invertebrates: 100 (68, 140) ng/g ww; fish: 160 (120, 210) ng/g ww; p < 0.05). The

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average concentrations of ΣSAs in benthic fish (260 ± 130 ng/g ww) was significantly

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higher (p < 0.05) than those in pelagic fish (120 (86, 160) ng/g ww). No statistically

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significant difference in ΣFQ levels was observed for benthic vs. pelagic fish (benthic

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fish: 89 ± 33 ng/g ww; pelagic fish: 43 (24, 77) ng/g ww; p > 0.05). Overall, benthic

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fish accumulates more SAs than invertebrates and pelagic fish, whereas invertebrates

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exhibit higher FQ levels than fish. Several factors, including different habits and/or 10


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antibiotic partition between water and sediments, likely explain differences in levels

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of the antibiotic classes investigated. For example, the higher concentrations of

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antibiotics in invertebrates and benthic fish are likely a result of their intimate contact

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with antibiotic-contaminated sediments. It is well established that antibiotics,

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especially FQs, can be adsorbed to organic matter in sediments.22 As a consequence,

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some FQs (e.g., ofloxacin, ciprofloxacin, norfloxacin and enrofloxacin) accumulate in

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sediments in many polluted environments.7,23

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Relative abundance (%)

100

SAs TMP FQs Invertebrates Benthic fish

MLs Pelagic fish

80 60 40 20

M M ac Sw an tra i tis qu mm shr Ja adr ing imp N Vpan R ang cra or e es az ul b th in e or ar er ed sto c is n r n la m ap e m ao a cr xi w ab a s he hr lk Pr imp Fa Oct awn Ja t gr opu ve ee s lin nl e in Fl go g at by Ch To Eel head n in gu go es e by e s so D Si ea p le ot lv ted er Merc gi y po ull h zz m et ar fr Had sh et lfb ad ea k

0

214 215

Figure 1. Abundance profiles of different antibiotic classes (percentage of the total measured

216

concentrations) reveal significant differences among invertebrates, benthic fish and pelagic fish.

217

SAs, TMP, FQs and MLs represent sulfonamides, trimethoprim, fluoroquinolones and macrolides,

218

respectively.

219 220

Antibiotic concentrations normalized for dry weight (Table S6) exhibited the same

221

trends observed for wet weight adjusted concentrations. Briefly, normalized ΣSA

222

concentrations (dw) were significantly higher (p < 0.05) in fish than those in 11



223

invertebrates, whereas no statistically significant difference was observed between

224

benthic and pelagic fish (p > 0.05). Moreover, there were no significant differences (p >

225

0.05) in total antibiotic or ΣFQ concentrations (dw) between fish and invertebrates or

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between benthic and pelagic fish. The moisture content of all organisms ranged from

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41% for fat greenling to 83% for octopus and significantly correlated (p < 0.05) with

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dry weight normalized concentrations of ΣMLs. No significant correlations (p > 0.05)

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were observed between moisture content and wet weight normalized antibiotic

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concentrations. Significant positive relationships (p < 0.05) were identified between

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sizes (lengths and weights) and wet weight concentrations of ΣSAs.

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A comparison of antibiotic levels in the organisms collected from the Laizhou Bay

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with levels reported in earlier studies from China reveals several distinct differences

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(Table S7). The levels of ΣSAs in all the species from Laizhou Bay were much higher

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than those reported for aquatic organisms from coastal areas of Dalian6 and Hailing

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Island.24 Levels of ΣFQs in organisms from Laizhou Bay were also higher than those

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reported for Haihe River10 and coastal areas of Hailing Island.24 Levels of these three

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antibiotic classes in fish from Laizhou Bay were higher than those detected in fish

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from rivers of the Pearl River Delta.25 In contrast, levels of ΣMLs in this study were

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much lower than those reported in organisms from Baiyangdian Lake,7 Bohai Sea,9

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Haihe River,10 and coastal areas of Hailing Island.24 Levels of Σ SAs and ΣFQs in

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biota samples from Laizhou Bay were similar to those from Baiyangdian Lake.7 The

243

different levels for antibiotics in different parts of China are most likely related to

244

regional differences in the use of antibiotics.2

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Only limited information regarding levels of antibiotics in global biotas is currently

246

available (Table S7). The levels of Σ FQs (nd to 0.73 ng/g ww) in shrimps and fish 12


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collected from Canada fish markets were much lower than those reported from

248

China.26 In mussels from the coastal areas of Ireland, the concentrations of

249

trimethoprim were up to 9.22 ng/g dw, which is similar to those observed in shellfish

250

(2.0 to 9.7 ng/g dw) in the present study.29 Sulfamethoxazole and trimethoprim, the

251

only two antibiotics analyzed, were not detected in any sample in national monitoring

252

studies conducted in Germany and the United States.27,28

253 254

Food Web Structure. To characterize food web relationships of the aquatic

255

organisms collected from Laizhou Bay and assess the trophodynamics of antibiotics,

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TLs and relative carbon sources were calculated using adjusted δ13C and δ15N values

257

(Figure S2). Adjusted δ13C values ranged from -22.8 ± 0.1 for eelgoby to -13.2 ± 2.3

258

for mullet. The δ15N values ranged from 6.6 ± 0.4 for northern maoxia shrimp to 14.6

259

± 0.1 for silvery pomfret. Estimated TLs ranged from 2.00 ± 0.10 for northern maoxia

260

shrimp to 4.10 ± 0.02 for silvery pomfret (Figure 2). Apparent intra-species

261

variability in TL values was observed in this study and, as has been suggested by

262

previous studies, may be attributed to the variation in the body sizes of the collected

263

aquatic organisms.18,30,31 No significant seasonal variation was found in the TL values

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of consumers (Table S8, p > 0.05).

265

Relative carbon sources of these species were used to assess if different species

266

belong to the same food web.21 Relative carbon source values close to 1 are thought to

267

indicate more pelagic feeding, whereas values close to 0 indicate more benthic

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feeding.21 Values of relative carbon sources ranged from -0.40 ± 0.30 for dotted

269

gizzard shad to 1.40 ± 0.01 for fat greenling (Figure 2). All species investigated were

270

distributed close to the mean value of the relative carbon sources of 0.4 (vertical line

271

in Figure 2). Eelgoby (Odontamblyopus rubicundus; 2.70 ± 0.05) and mullet (Liza 13



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haematocheilus; -2.40 ± 0.03) were an exception, which indicates that their diets

273

might include sources which do not belong to the food web under investigation.

274

Therefore, both species were not included in the calculation of trophic magnification

275

factors (TMF) in this study.

276

5.0

Invertebrates Benthic fish Pelagic fish

4.5

Trophic level (TL)

Chinese sea perch

Javeline goby

Silvery pomfret

4.0

Flathead Mantis shrimp Swimming crab

3.5 Mullet 3.0

Prawn Dotted gizzard shad

2.5 Halfbeak 2.0

Fat greenling

Eelgoby

Octopus Japanese stone crab Tongue sole

Mactra quadrangularis Razor clam Veined rapa whelk

Northern maoxia shrimp -2

-1

0 1 Relative carbon source

2

3

277 278

Figure 2. Relative carbon sources and trophic levels (TL) characterize the food web relationships

279

of the species collected from Laizhou Bay. Green circles (), blue diamonds () and black

280

triangles () represent invertebrates, benthic fish and pelagic fish, respectively. Data are

281

presented as mean ± one standard deviation of relative carbon sources (n = 3) and TL values (n =

282

3). The red vertical solid line and dotted lines represent the mean value of relative carbon sources

283

for all species and the boundaries of relative carbon sources for species in the same food web.32

284 285

Trophic Transfer of Antibiotics in the Marine Food Web. TMFs of individual

286

antibiotics and the three antibiotic classes (i.e, SAs, FQs, and MLs) in the food web

287

were determined from the slope of the regression between log-transformed antibiotic 14


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concentrations (ww) in biota and the TLs of the corresponding species (Figure 3;

289

Table S9). Overall, the TMF values followed the order SAs ≥ trimethoprim > MLs >

290

FQs.

291

sulfamonomethoxine, sulfachlorpyridazine, sulfadimidine, and ∑SAs increased

292

significantly with increasing TLs (p < 0.05). Similarly, a significant positive

293

correlation (p < 0.05) was observed between the TL values and trimethoprim

294

concentrations (ww). The TMF values were 1.2 - 3.9 for SAs and 2.4 for trimethoprim.

295

These TMF values are greater than those reported previously for other

296

pharmaceuticals, such as diphenhydramine (TMF = 0.38) and carbamazepine (TMF =

297

1.17) in an effluent stream from the North Bosque River, USA and propranolol (TMF

298

= 0.31) and diclofenac (TMF = 1.06) in a freshwater food web from Taihu Lake,

299

China.18,31 A similar trophic transfer for SAs was also observed in Baiyangdian Lake,

300

North China, where fish (nd - 98.3 ng/g dw, mean: 20.8 ng/g dw) had the highest

301

concentrations of SAs, followed by crustaceans (nd - 30.6 ng/g dw, 11.7 ng/g dw) and

302

then aquatic plants (nd -15 ng/g dw, 1.67 ng/g dw).7 These results demonstrate that

303

SAs and trimethoprim, like other pharmaceuticals, have the potential to biomagnify in

304

a marine food web. Previous studies have shown that SAs such as sulfamethoxazole

305

and trimethoprim are well absorbed in the intestinal tracts33-35 but not efficiently

306

metabolized in aquatic organisms, such as crustaceans, mollusks, fish.35-38 Therefore,

307

trophic magnification of SAs and trimethoprim in this marine food web is likely due

308

to their low metabolic transformation and efficient assimilation in animals at higher

309

trophic levels.

For

SAs,

concentrations

(ww)

of

sulfadiazine,

15


sulfamethoxazole,


log concentration (ng/g ww)

3.0 (a) Sulfamethoxazole

y = 0.34x + 0.60 r = 0.85, p < 0.05 TMF = 2.19

2.5

1.2

1.5

0.0

3


3.5 (c) Enrofloxacin

4

2

y = -0.40x + 3.44 r = 0.76, p < 0.05 TMF = 0.40

3.0

3

3.0 (d) Ofloxacin

2.5

2.0

2.0

1.5

4 y = -0.42x + 2.91 r = 0.77, p < 0.05 TMF = 0.38

2.5

1.0

1.5 2


y = 0.38x - 0.85 r = 0.54, p < 0.05 TMF = 2.40

-1.2 2

0.8

(b) Trimethoprim

2.4

2.0

1.0

3

(e) Azithromycin

4

2

y = -0.16x + 0.73 r = 0.88, p < 0.05 TMF = 0.70

0.6

0.2

0.0

0.0

-0.5 4

4

(f) Anhydroerythromycin y = -0.20x + 0.97 r = 0.66, p < 0.05 TMF = 0.64 1.0

0.5

3

3

1.5

0.4

2

310

3.6

Page 16 of 33

2

Trophic level (TL)

3 Trophic level (TL)

4

311

Figure 3. Relationships between trophic levels (TL) and log-transformed wet weight normalized

312

concentrations (ww) describe trophic magnification for (a) sulfamethoxazole, (b) trimethoprim

313

and trophic dilution for (c) enrofloxacin, (d) ofloxacin, (e) azithromycin and (f)

314

anhydroerythromycin in the marine food web from Laizhou Bay. Slopes, correlation coefficients

315

(r), p-values and trophic magnification factors (TMF) from the regression analyses are shown

316

(Table S9 for additional details). Data are presented as mean ± one standard deviation of TL

16


Page 17 of 33


317

values (n = 3) and log-transformed antibiotic concentrations which were detected in at least two

318

out of three homogenized samples.

319 320

The TMF values ranged from 0.3 to 0.8 for FQs and from 0.5 to 1.0 for MLs,

321

respectively. Significant negative relationships (p < 0.05) were observed for

322

correlations between TL values and concentrations of ΣFQs and ΣMLs. Moreover,

323

there were significant negative correlations (p < 0.05) between the TL values and

324

concentrations of three FQs (i.e., enoxacin, enrofloxacin, and ofloxacin) and two MLs

325

(i.e., azithromycin and anhydroerythromycin) in the species under investigation.

326

These findings demonstrate that both antibiotic classes undergo trophic dilution in the

327

marine food web of Laizhou Bay. Literature TMF values for both classes of

328

antibiotics are limited and only TMF values for roxithromycin have been reported.15,18

329

In this study, the TMF values for azithromycin (0.7, 95% CI: 0.5 - 0.9) and

330

anhydroerythromycin (0.6, 95% CI: 0.5 - 0.9) were higher than values reported for

331

roxithromycin (0.21 - 0.29) in an artificial aquatic food chain but slight lower than

332

values for roxithromycin (1.11) in a freshwater food web from Taihu Lake, China.15,18

333

Unfortunately, the detection frequency of roxithromycin (23%) was too low to

334

calculate a TMF value in this study (Table S5). For the other seven antibiotics, no

335

significant correlations (p > 0.05) were observed between TL values and their wet

336

weight concentrations.

337 338

Influence of Physicochemical Factors on Trophic Magnification of Antibiotics.

339

Octanol-water partition coefficients (Kow) are excellent predictors of the

340

biomagnification of hydrophobic organic compounds in aquatic food webs.39 The

341

TMF values of the antibiotics investigated in this study decreased with increasing 17



342

logKOW; however, this correlation was not statistically significant (p > 0.05, Figure

343

4a). Negative relationships or no relationships between TMF and logKOW values are

344

also observed for phthalate esters,40 and some hydrophobic compounds (e.g., dioxins,

345

polychlorinated biphenyls).41-43 No significant correlation between TMFs of these

346

antibiotics and their logKOW values is not entirely surprising because the antibiotics

347

investigated are polar molecules and contain ionizable functional groups. Like other

348

ionic compounds, an ionic form of an antibiotic cannot diffuse across cell membranes

349

and is not readily absorbed in the intestinal tracts of marine organisms.29,44 Therefore,

350

logKOW is not a good biomagnification predictor for these antibiotics. We also

351

investigated if measures of the fraction of the non-ionic form of an antibiotic, such as

352

the fraction of neutral molecules (fn) and the pH dependent distribution coefficient

353

(logD),45 are better predictors of its biomagnification in a marine food web. Indeed,

354

we found that the SAs and trimethoprim with higher TMF values displayed higher fn

355

values (Table S1, p < 0.05) than the FQs and MLs (Figure S3). Furthermore, a

356

significant positive correlation (r = 0.73; p < 0.05) was observed between TMFs of the

357

antibiotics and their logD (pH = 7.5, Table S1) values, with the exception of the three

358

ML antibiotics (Figure 4b). A similar correlation between BCF values of other

359

pharmaceuticals and their pH-corrected liposome-water partition coefficients was

360

observed in some invertebrates.46 The correlation suggests that logD of ionizable

361

organic chemicals, such as antibiotics, is a better predictor of their biomagnification

362

potential in marine food webs than logKOW.

363

18


Page 18 of 33


8 6

Trophic magnification factor (TMF)

Trophic magnification factor (TMF)

Page 19 of 33

SAs FQs TMP MLs

(a) y = -0.16x + 1.72 p > 0.05

4 2 0

0

1

2

3

4

8 6 4 2 0

-1.00 -0.75 -0.50 -0.25

logKOW

364

SAs FQs TMP

(b) y = 2.21x + 2.52 r = 0.73, p < 0.05

0.00

0.25

logD

365

Figure 4. Relationships between TMFs of detected antibiotics and physicochemical factors

366

(logKOW (a) and logD values (b)). The TMF values for SAs, trimethoprim, FQs and MLs are

367

indicated by blue triangles (), red circles (), green inverted triangles () and orange diamonds

368

(), respectively. Equations, correlation coefficients (r) and p-values of the regression analyses

369

are shown. LogKOW and logD values of antibiotics (Table S1) were collected from elsewhere.

370 371

Biotransformation processes within the food web are one possible explanation for

372

the lack of a correlation between TMFs and logD values for the ML antibiotics.

373

Although biotransformation was not directly assessed in this study, there is some

374

literature evidence demonstrating that aquatic organisms have the capacity to

375

metabolize ML antibiotics.47-49 For example, Liu et al. reported that erythromycin and

376

roxithromycin are readily metabolized by crucian carp (Carassius auratus) and

377

eliminated via the bile, most likely due to the induction of cytochrome P450 enzymes

378

(measured

379

exposure.47,48 Chen et al. observed faster hepatic clearance rates for tertiary amines

380

(e.g., MLs) than other amines (e.g., SAs) in liver S9 fractions of rainbow trout

381

(Oncorhynchus mykiss).49 Unfortunately, the biotransformation of antibiotics by

382

aquatic organisms at different TLs is not fully understood and future studies are

383

needed.

as

ethoxyresorufin-O-deethylase

activity)

19


following

antibiotic


384

Assessment of Human Health Risks. All species investigated in this study are

385

caught for human consumption.50-52 Estimated daily intakes (EDI, Table S10) for each

386

antibiotic following consumption of seafood were calculated assuming a worst-case

387

scenario for rural and urban residents in China.53 For urban residents, EDIs of

388

antibiotics ranged from 0.5 ng/kg bw/d for roxithromycin to 144 ng/kg bw/d for

389

enrofloxacin. EDIs for rural residents ranged from 0.3 ng/kg bw/d for roxithromycin

390

to 78 ng/kg bw/d for enrofloxacin. The EDIs for urban residents were two times

391

higher than those for rural residents due to the higher fish consumption of urban

392

residents.54 All EDI values were two to five orders of magnitude lower than the

393

respective acceptable daily intakes (ADI, Table S10). Based on the EDI values and

394

the respective ADIs, hazard quotients of individual antibiotics (HQ; Table S10,

395

Figure S4) were calculated for urban and rural residents and used to assess potential

396

human health risks from dietary exposure to antibiotics.53 Vragovic et al. suggested

397

that a HQ < 0.01 indicates a negligible risk whereas a HQ ≥ 0.01 indicates a

398

considerable risk.55 A HQ > 0.05 is considered a distinct risk.55 The HQ of

399

enrofloxacin for urban residents (HQ = 0.07, i.e., 7% of ADI) exceeded 0.05 (Figure

400

S4). The HQs of ciprofloxacin (HQ = 0.01, i.e., 1% of ADI) and clarithromycin (HQ =

401

0.01, i.e., 1% of ADI) for urban residents and enrofloxacin for rural residents (HQ =

402

0.04, i.e., 4% of ADI) were ≥ 0.01. These results suggest that there is an appreciable

403

human health risk associated with the exposure to antibiotics due to consumption of

404

seafood from Laizhou Bay.

405

We also calculated hazard index values (HI = ΣHQi) assuming a similar

406

toxicological mode of action (MOA) for substances belong to the same class to assess

407

if consumption of seafood from Laizhou Bay represents a human health risk.53 The HI

20


Page 20 of 33

Page 21 of 33


408

values of ΣMLs for urban residents (HI = 0.014) exceeded 0.01. The HI values of

409

ΣFQs were 0.094 and 0.050 for urban and rural residents, respectively (Table S10).

410

The high risk of FQs in seafood represents a potential concern because of the

411

relatively low ADIs (1,600 to 11,400 ng/kg bw/d) and high EDIs (8.6 to 144 ng/kg

412

bw/d). Compared to FQs and MLs, SAs exhibited relatively high biomagnification

413

potential but represented a small ingestion risk (i.e., the HI values of SAs are < 0.01).

414

Exposure Implications. Due to intensive local aquaculture activities, ambient

415

wastewater discharge and runoff from farming, large amounts of antibiotic residues

416

have been discharged into the water bodies of Laizhou Bay,4,56,57 which increases the

417

possibility of bioaccumulation and biomagnification of antibiotics in aquatic

418

organisms. Our comprehensive investigation of the trophodynamics of antibiotics in a

419

marine food web from this region demonstrates that several antibiotics undergo

420

trophic transfer to top predators or low-trophic level invertebrates and accumulate in

421

certain tissues, such as the liver and blood.25 In order to reduce the potential exposure

422

risks, more strict maximal residue limits (MRL) of antibiotics in seafood need to be

423

set for Chinese residents. While consumption of seafood alone is unlikely to represent

424

a major human health risk in China, humans are exposed to antibiotics by a variety of

425

other exposure pathways, such as other food intakes,58,59 drug abuse,60 tap water,61 and

426

inhalation of contaminated dust.62 Moreover, this study did not investigate levels of

427

antibiotic metabolites in the food web from Laizhou Bay. Emerging evidence suggests

428

that the biotransformation products of antibiotics may be more toxic (e.g.,

429

protein-reactive) and/or bioaccumulative than the parent compounds.63-65 For example,

430

sulfamethoxazole can be hydroxylated and further oxidized in humans and rats to

431

nitroso-sulfamethoxazole, which can bind covalently to cysteine residues of cellular

432

proteins and cause toxicity.63,64 Furthermore, decarboxylation of antibiotics, such as 21



433

FQs and cephalosporins, may generate more fat-soluble metabolites that are more

434

bioaccumulative than the parent antibiotics.65 In addition, ecotoxicological studies

435

suggest that some antibiotics investigated (e.g., ciprofloxacin, erythromycin,

436

sulfamethoxazole and trimethoprim) can induce phototoxicity to Daphnia magna,

437

disrupt the immune system in mollusks, or impair the development of fish in their

438

early life stages.66-68 Therefore, the environmental behavior of antibiotics in aquatic

439

food webs and potential ecological and human health risks associated with exposure

440

to antibiotics and their metabolites warrant further attention.

441 442

ASSOCIATED CONTENT

443

Supporting Information. Text, figures, and tables addressing (1) physicochemical

444

properties of selected antibiotics in this study; (2) chemicals used in the analysis; (3)

445

details about sample extraction and cleanup, and stable isotope analysis; (4)

446

calculations of TMFs, relative carbon sources, estimated daily intakes (EDI) and

447

hazard quotients (HQ); (5) sampling figure and tables about sample information; (6)

448

table about MLODs, MLOQs, spiked recoveries and RSD of target antibiotics; (7)

449

table about LC-MS/MS parameters and retention times of antibiotics; (8) tables about

450

detection frequencies and concentrations in biota samples; (9) table about slopes, r

451

and p-values of regression analyses between logarithm concentrations and TLs, and

452

TMF values of antibiotics; (10) table about the comparison of antibiotic levels in

453

aquatic organisms from Laizhou Bay with those reported in other locations; (11) table

454

and figure about EDIs and HQs of antibiotics for rural and urban residents; (12) figure

455

about relationship between TMFs and logKOW values. This material is available free of

456

charge via the Internet at http://pubs.acs.org.

22


Page 22 of 33

Page 23 of 33


457

AUTHOR INFORMATION

458

Corresponding Authors

459

* Hongxia Zhao, phone/fax: +86-411-8470 7965, e-mail: [email protected],

460

address: Linggong Road 2, Ganjingzi District, Dalian 116024, China; Jingwen Chen,

461

phone/fax: +86-411-8470 6269, e-mail: [email protected], address: Linggong Road

462

2, Ganjingzi District, Dalian 116024, China.

463 464

ACKNOWLEDGEMENT

465

This study was supported by the National Basic Research Program of China

466

(2013CB430403), the National Natural Science Foundation of China (21325729,

467

21277017) and the Basic Research Project of Key Laboratory of Liaoning Provincial

468

Education Department (LZ2015023).

23



Page 24 of 33

469

REFERENCES

470

(1) Zhu, Y. G.; Johnson, T. A.; Su, J. Q.; Qiao, M.; Guo, G. X.; Stedtfeld, R. D.; Hashsham, S. A.;

471

Tiedje, J. M. Diverse and abundant antibiotic resistance genes in Chinese swine farms. P. Natl. Acad.

472

Sci. USA. 2013, 110 (9), 3435-3440; DOI 10.1073/pnas.1222743110.

473

(2) Zhang, Q. Q.; Ying, G. G.; Pan, C. G.; Liu, Y. S.; Zhao, J. L. Comprehensive evaluation of

474

antibiotics emission and fate in the river basins of China: Source analysis, multimedia modelling, and

475

linkage to bacterial resistance. Environ. Sci. Technol. 2015, 49 (11), 6772-6782; DOI

476

10.1021/acs.est.5b00729.

477

(3) Bound, J. P.; Voulvoulis, N. Pharmaceuticals in the aquatic environment - a comparison of risk

478

assessment

479

10.1016/j.chemosphere.2004.05.010.

480

(4) Zou, S. C.; Xu, W. H.; Zhang, R. J.; Tang, J. H.; Chen, Y. J.; Zhang, G. Occurrence and

481

distribution of antibiotics in coastal water of the Bohai Bay, China: Impacts of river discharge and

482

aquaculture activities. Environ. Pollut. 2011, 159 (10), 2913-2920; DOI 10.1016/j.envpol.2011.04.037.

483

(5) Shi, H.; Yang, Y.; Liu, M.; Yan, C. X.; Yue, H. Y.; Zhou, J. L. Occurrence and distribution of

484

antibiotics in the surface sediments of the Yangtze Estuary and nearby coastal areas. Mar. Pollut. Bull.

485

2014, 83 (1), 317-323; DOI 10.1016/j.marpolbul.2014.04.034.

486

(6) Na, G. S.; Fang, X. D.; Cai, Y. Q.; Ge, L. K.; Zong, H. M.; Yuan, X. T.; Yao, Z. W.; Zhang, Z. F.

487

Occurrence, distribution, and bioaccumulation of antibiotics in coastal environment of Dalian, China.

488

Mar. Pollut. Bull. 2013, 69 (1-2), 233-237; DOI 10.1016/j.marpolbul.2012.12.028.

489

(7) Li, W. H.; Shi, Y. L.; Gao, L. H.; Liu, J. M.; Cai, Y. Q. Occurrence of antibiotics in water,

490

sediments, aquatic plants, and animals from Baiyangdian Lake in North China. Chemosphere 2012, 89

strategies.

Chemosphere

2004,

56

(11),

24


1143-1155;

DOI

Page 25 of 33


491

(11), 1307-1315; DOI 10.1016/j.chemosphere.2012.05.079.

492

(8) Mackay, D.; Hughes, D. M.; Romano, M. L.; Bonnell, M. The role of persistence in chemical

493

evaluations. Integr. Environ. Assess. Manag. 2014, 10 (4), 588-594; DOI 10.1002/ieam.1545.

494

(9) Li, W. H.; Shi, Y. L.; Gao, L. H.; Liu, J. M.; Cai, Y. Q. Investigation of antibiotics in mollusks

495

from coastal waters in the Bohai Sea of China. Environ. Pollut. 2012, 162, 56-62; DOI

496

10.1016/j.envpol.2011.10.022.

497

(10) Gao, L. H.; Shi, Y. L.; Li, W. H.; Liu, J. M.; Cai, Y. Q. Occurrence, distribution and

498

bioaccumulation of antibiotics in the Haihe River in China. J. Environ. Monitor. 2012, 14 (4),

499

1248-1255; DOI 10.1039/C2em10916f.

500

(11) He, X. T.; Wang, Z. H.; Nie, X. P.; Yang, Y. F.; Pan, D. B.; Leung, A. O. W.; Cheng, Z.; Yang, Y. T.;

501

Li, K. B.; Chen, K. C. Residues of fluoroquinolones in marine aquaculture environment of the Pearl

502

River

503

10.1007/s10653-011-9420-4.

504

(12) Zhao, H. X.; Liu, S. S.; Chen, J. W.; Jiang, J. Q.; Xie, Q.; Quan, X. Biological uptake and

505

depuration of sulfadiazine and sulfamethoxazole in common carp (Cyprinus carpio). Chemosphere

506

2015, 120, 592-597; DOI 10.1016/j.chemosphere.2014.09.075.

507

(13) Zhang, K.; Wan, Y.; Jones, P. D.; Wisemans, S.; Giesy, J. P.; Hu, J. Y. Occurrences and fates of

508

hydroxylated polybrominated diphenyl ethers in marine sediments in relation to trophodynamics.

509

Environ. Sci. Technol. 2012, 46 (4), 2148-2155; DOI 10.1021/es203195s.

510

(14) Boonsaner, M.; Hawker, D. W., Evaluation of food chain transfer of the antibiotic oxytetracycline

511

and

512

10.1016/j.chemosphere.2013.05.070.

Delta,

human

South

risk

China.

Environ.

assessment.

Geochem.

Chemosphere

Health

2013,

2012,

93

25


34

(6),

(3),

323-335;

1009-1014;

DOI

DOI


513

(15) Ding, J. N.; Lu, G. H.; Liu, J. C.; Zhang, Z. H. Evaluation of the potential for trophic transfer of

514

roxithromycin along an experimental food chain. Environ. Sci. Pollut. Res. 2015, 22 (14), 10592-10600;

515

DOI 10.1007/s11356-015-4265-5.

516

(16) Post, D. M.; Layman, C. A.; Arrington, D. A.; Takimoto, G.; Quattrochi, J.; Montana, C. G.

517

Getting to the fat of the matter: Models, methods and assumptions for dealing with lipids in stable

518

isotope analyses. Oecologia. 2007, 152 (1), 179-189; DOI 10.1007/s00442-006-0630-x.

519

(17) Ma, X. D.; Zhang, H. J.; Wang, Z.; Yao, Z. W.; Chen, J. W.; Chen, J. P. Bioaccumulation and

520

trophic transfer of short chain chlorinated paraffins in a marine food web from Liaodong Bay, North

521

China. Environ. Sci. Technol. 2014, 48 (10), 5964-5971; DOI 10.1021/Es500940p.

522

(18) Xie, Z. X.; Lu, G. H.; Liu, J. C.; Yan, Z. H.; Ma, B. N.; Zhang, Z. H.; Chen, W. Occurrence,

523

bioaccumulation, and trophic magnification of pharmaceutically active compounds in Taihu Lake,

524

China. Chemosphere 2015, 138, 140-147; DOI 10.1016/j.chemosphere.2015.05.086.

525

(19) Cui, Y.; Wu, Y.; Xu, Z. L.; Zhang, J. Potential dietary influence on the stable isotopes and fatty

526

acid composition of migratory anchovy (Coilia mystus) around the Changjiang Estuary. J. Mar. Biol.

527

Assoc. UK. 2015, 95 (1), 193-205; DOI 10.1017/S0025315414000873.

528

(20) Cabana, G.; Rasmussen, J. B. Comparison of aquatic food chains using nitrogen isotopes. P. Natl.

529

Acad. Sci. USA. 1996, 93 (20), 10844-10847; DOI 10.1073/pnas.93.20.10844.

530

(21) McKinney, M. A.; McMeans, B. C.; Tomy, G. T.; Rosenberg, B.; Ferguson, S. H.; Morris, A.;

531

Muir, D. C. G.; Fisk, A. T. Trophic transfer of contaminants in a changing arctic marine food web:

532

Cumberland Sound, Nunavut, Canada. Environ. Sci. Technol. 2012, 46 (18), 9914-9922; DOI

533

10.1021/es302761p.

534

(22) Tolls, J. Sorption of veterinary pharmaceuticals in soils: A review. Environ. Sci. Technol. 2001, 35

26


Page 26 of 33

Page 27 of 33


535

(17), 3397-3406; DOI 10.1021/es0003021.

536

(23) Kim, S. C.; Carlson, K. Temporal and spatial trends in the occurrence of human and veterinary

537

antibiotics in aqueous and river sediment matrices. Environ. Sci. Technol. 2007, 41 (1), 50-57; DOI

538

10.1021/es060737+.

539

(24) Chen, H.; Liu, S.; Xu, X. R.; Liu, S. S.; Zhou, G. J.; Sun, K. Y.; Zhao, J. L.; Ying, G. G.

540

Antibiotics in typical marine aquaculture farms surrounding Hailing Island, South China: Occurrence,

541

bioaccumulation and human dietary exposure. Mar. Pollut. Bull. 2015, 90 (1-2), 181-187; DOI

542

10.1016/j.marpolbul.2014.10.053.

543

(25) Zhao, J. L.; Liu, Y. S.; Liu, W. R.; Jiang, Y. X.; Su, H. C.; Zhang, Q. Q.; Chen, X. W.; Yang, Y. Y.;

544

Chen, J.; Liu, S. S.; Pan, C. G.; Huang, G. Y.; Ying, G. G. Tissue-specific bioaccumulation of human

545

and veterinary antibiotics in bile, plasma, liver and muscle tissues of wild fish from a highly urbanized

546

region. Environ. Pollut. 2015, 198, 15-24; DOI 10.1016/j.envpol.2014.12.026.

547

(26) Tittlemier, S. A.; Van de Riet, J.; Burns, G.; Potter, R.; Murphy, C.; Rourke, W.; Pearce, H.;

548

Dufresne, G. Analysis of veterinary drug residues in fish and shrimp composites collected during the

549

Canadian Total Diet Study, 1993-2004. Food Addit. Contam. 2007, 24 (1), 14-20; DOI

550

10.1080/02652030600932937.

551

(27) Subedi, B.; Du, B. W.; Chambliss, C. K.; Koschorreck, J.; Rudel, H.; Quack, M.; Brooks, B. W.;

552

Usenko, S. Occurrence of pharmaceuticals and personal care products in German fish tissue: A national

553

study. Environ. Sci. Technol. 2012, 46 (16), 9047-9054; DOI 10.1021/es301359t.

554

(28) Ramirez, A. J.; Brain, R. A.; Usenko, S.; Mottaleb, M. A.; O'Donnell, J. G.; Stahl, L. L.; Wathen, J.

555

B.; Snyder, B. D.; Pitt, J. L.; Perez-Hurtado, P.; Dobbins, L. L.; Brooks, B. W.; Chambliss, C. K.

556

Occurrence of pharmaceuticals and personal care products in fish: Results of a national pilot study in

27



Page 28 of 33

557

the United States. Environ. Toxicol. Chem. 2009, 28 (12), 2587-2597; DOI 10.1897/08-561.1.

558

(29) McEneff, G.; Barron, L.; Kelleher, B.; Paull, B.; Quinn, B. A year-long study of the spatial

559

occurrence and relative distribution of pharmaceutical residues in sewage effluent, receiving marine

560

waters

561

10.1016/j.scitotenv.2013.12.123.

562

(30) Borga, K.; Fjeld, E.; Kierkegaard, A.; McLachlan, M. S. Consistency in trophic magnification

563

factors of cyclic methyl siloxanes in pelagic freshwater food webs leading to brown trout. Environ. Sci.

564

Technol. 2013, 47 (24), 14394-14402; DOI 10.1021/es404374j.

565

(31) Du, B.; Haddad, S. P.; Luek, A.; Scott, W. C.; Saari, G. N.; Kristofco, L. A.; Connors, K. A.; Rash,

566

C.; Rasmussen, J. B.; Chambliss, C. K.; Brooks, B. W. Bioaccumulation and trophic dilution of human

567

pharmaceuticals across trophic positions of an effluent-dependent wadeable stream. Philos. Trans. R.

568

Soc. London, Ser. B. 2014, 369 (1656), 1-10; DOI Artn 2014005810.1098/Rstb.2014.0058.

569

(32) Jia, H. L.; Zhang, Z. F.; Wang, C. Q.; Hong, W. J; Sun, Y. Q.; Li, Y. F. Trophic transfer of methyl

570

siloxanes in the marine food web from coastal area of Northern China. Environ. Sci. Technol. 2015, 49

571

(5), 2833-2840; DOI 10.1021/es505445e.

572

(33) Klopman, G.; Stefan, L. P.; Saiakhov, R. D. ADME evaluation: 2. A computer model for the

573

prediction of intestinal absorption in humans. Eur. J. Pharm. Sci. 2002, 17 (4-5), 253-263; DOI

574

10.1016/S0928-0987(02)00219-1.

575

(34) Meshi, T.; Sato, Y. Studies on sulfamethoxazole/trimethoprim: absorption, distribution, excretion

576

and metabolism of trimethoprim in rat. Chem. Pharm. Bullet. 1972, 20 (10), 2079-2090; DOI

577

10.1248/cpb.20.2079.

578

(35) Touraki, M.; Niopas, I.; Kastritsis, C. Bioaccumulation of trimethoprim, sulfamethoxazole and

and

marine

bivalves.

Sci.

Total

Environ.

2014,

28


476,

317-326;

DOI

Page 29 of 33


579

N-acetyl-sulfamethoxazole in Artemia nauplii and residual kinetics in seabass larvae after repeated oral

580

dosing

581

10.1016/S0044-8486(99)00036-8.

582

(36) Connors, K. A.; Du, B. W.; Fitzsimmons, P. N.; Hoffman, A. D.; Chambliss, C. K.; Nichols, J. W.;

583

Brooks, B. W. Comparative pharmaceutical metabolism by rainbow trout (Oncorhynchus mykiss) liver

584

S9 fractions. Environ. Toxicol. Chem. 2013, 32 (8), 1810-1818; DOI 10.1002/etc.2240.

585

(37) Nouws, J. F. M.; et al. Pharmacokinetics, hydroxylation and acetylation of sulphadimidine in

586

mammals, birds, fish, reptiles and molluscs. In Comparative Veterinary Pharmacology, Toxicology

587

and Therapy; Van Miert, A. S. J. P. A. M., Bogaert, M. G., Debackere M., Eds.; MTP Press:

588

Lancaster, England 1985; pp 301-318.

589

(38) Chair, M.; Nelis, H. J.; Leger, P.; Sorgeloos, P.; de Leenheer, A. P. Accumulation of trimethoprim,

590

sulfamethoxazole, and N-acetylsulfamethoxazole in fish and shrimp fed medicated Artemia franciscana.

591

Antimicrob. Agents Chemother. 1996, 40 (7), 1649-1652.

592

(39) Kelly, B. C.; Ikonomou, M. G.; Blair, J. D.; Morin, A. E.; Gobas, F. A. P. C. Food web-specific

593

biomagnification of persistent organic pollutants. Science 2007, 317 (5835), 236-239; DOI

594

10.1126/science.1138275.

595

(40) Mackintosh, C. E.; Maldonado, J.; Jing, H. W.; Hoover, N.; Chong, A.; Ikonomou, M. G.; Gobas,

596

F. A. P. C. Distribution of phthalate esters in a marine aquatic food web: Comparison to polychlorinated

597

biphenyls. Environ. Sci. Technol. 2004, 38 (7), 2011-2020; DOI 10.1021/es034745r.

598

(41) Kobayashi, J.; Imuta, Y.; Komorita, T.; Yamada, K.; Ishibashi, H.; Ishihara, F.; Nakashima, N.;

599

Sakai, J.; Arizono, K.; Koga, M. Trophic magnification of polychlorinated biphenyls and

600

polybrominated diphenyl ethers in an estuarine food web of the Ariake Sea, Japan. Chemosphere 2015,

of

medicated

nauplii.

Aquaculture.

1999,

175

29


(1-2),

15-30;

DOI


601

118, 201-206; DOI 10.1016/j.chemosphere.2014.08.066.

602

(42) Khairy, M. A.; Weinstein, M. P.; Lohmann, R. Trophodynamic behavior of hydrophobic organic

603

contaminants in the aquatic food web of a tidal river. Environ. Sci. Technol. 2014, 48 (21),

604

12533-12542; DOI 10.1021/es502886n.

605

(43) Zheng, G. M. W., Y.; Hu, J. Y. Intrinsic clearance of xenobiotic chemicals by liver microsomes:

606

Assessment of trophic magnification potentials. Environ. Sci. Technol. 2016, 50 (12), 6343-6353; DOI

607

10.1021/acs.est.6b01178.

608

(44) Erickson, R. J.; McKim, J. M.; Lien, G. J.; Hoffman, A. D.; Batterman, S. L. Uptake and

609

elimination of ionizable organic chemicals at fish gills: II. Observed and predicted effects of pH,

610

alkalinity, and chemical properties. Environ. Toxicol. Chem. 2006, 25 (6), 1522-1532; DOI

611

10.1897/05-359R.1.

612

(45) Fu, W. J.; Franco, A.; Trapp, S. Methods for estimating the bioconcentration factor of ionizable

613

organic chemicals. Environ. Toxicol. Chem. 2009, 28 (7), 1372-1379; DOI 10.1897/08-233.1.

614

(46) Meredith-Williams, M.; Carter, L. J.; Fussell, R.; Raffaelli, D.; Ashauer, R.; Boxall, A. B. A.

615

Uptake and depuration of pharmaceuticals in aquatic invertebrates. Environ. Pollut. 2012, 165, 250-258;

616

DOI 10.1016/j.envpol.2011.11.029.

617

(47) Liu, J. C.; Lu, G. H.; Ding, J. N.; Zhang, Z. H.; Wang, Y. H. Tissue distribution, bioconcentration,

618

metabolism, and effects of erythromycin in crucian carp (Carassius auratus). Sci. Total Environ. 2014,

619

490, 914-920; DOI 10.1016/j.scitotenv.2014.05.055.

620

(48) Liu, J. C.; Lu, G. H.; Wang, Y. H.; Yan, Z. H.; Yang, X. F.; Ding, J. N.; Jiang, Z. Bioconcentration,

621

metabolism, and biomarker responses in freshwater fish Carassius auratus exposed to roxithromycin.

622

Chemosphere 2014, 99, 102-108; DOI 10.1016/j.chemosphere.2013.10.036.

30


Page 30 of 33

Page 31 of 33


623

(49) Chen, Y.; Hermens, J. L. M.; Jonker, M. T. O.; Arnot, J. A.; Armitage, J. M.; Brown, T.; Nichols, J.

624

W.; Fay, K. A.; Droge, S. T. J. Which molecular features affect the intrinsic hepatic clearance rate of

625

ionizable organic chemicals in fish? Environ. Sci. Technol. 2016, ASAP; DOI 10.1021/acs.est.6b03504.

626

(50) Search fishbase; http://www.Fishbase.Se/search.php. Access date: Jun 2016.

627

(51) Tian, S. Y.; Zhu, L. Y.; Liu, M. Bioaccumulation and distribution of polybrominated diphenyl

628

ethers in marine species from Bohai Bay, China. Environ. Toxicol. Chem. 2010, 29 (10), 2278-2285;

629

DOI 10.1002/etc.275.

630

(52) Jin, J.; Liu, W. Z.; Wang, Y.; Tang, X. Y. Levels and distribution of polybrominated diphenyl

631

ethers in plant, shellfish and sediment samples from Laizhou Bay in China. Chemosphere 2008, 71 (6),

632

1043-1050; DOI 10.1016/j.chemosphere.2007.11.041.

633

(53) U.S. Environmental Protection Agency. Guidance for assessing chemical contaminant data for

634

use in fish advisories-Risk assessment and fish consumption limits, 3rd, ed.; Office of Water:

635

Washington, DC, 2000.

636

(54) National Burean of Statistics of China; http://data.stats.gov.cn. Access date: July 2016.

637

(55) Vragovic, N.; Bazulic, D.; Njari, B. Risk assessment of streptomycin and tetracycline residues in

638

meat and milk on Croatian market. Food Chem. Toxicol. 2011, 49 (2), 352-355; DOI

639

10.1016/j.fct.2010.11.006.

640

(56) Zhang, R. J.; Zhang, G.; Zheng, Q.; Tang, J. H.; Chen, Y. J.; Xu, W. H.; Zou, Y. D.; Chen, X. X.

641

Occurrence and risks of antibiotics in the Laizhou Bay, China: Impacts of river discharge. Ecotox.

642

Environ. Safe. 2012, 80, 208-215; DOI 10.1016/j.ecoenv.2012.03.002.

643

(57) Zhang, R. J.; Tang, J, H.; Li, J.; Zheng, Q.; Liu, D.; Chen, Y. J.; Zou, Y. D.; Chen, X. X.; Luo, C.

644

L.; Zhang, G. Antibiotics in the offshore waters of the Bohai Sea and the Yellow Sea in China:

31



Page 32 of 33

645

Occurrence,

646

10.1016/j.envpol.2012.11.008.

647

(58) Hu, X. G.; Zhou, Q. X.; Luo, Y. Occurrence and source analysis of typical veterinary antibiotics in

648

manure, soil, vegetables and groundwater from organic vegetable bases, Northern China. Environ.

649

Pollut. 2010, 158 (9), 2992-2998; DOI 10.1016/j.envpol.2010.05.023.

650

(59) Yamaguchi, T.; Okihashi, M.; Harada, K.; Konishi, Y.; Uchida, K.; Do, M. H. N.; Bui, H. D. T.;

651

Nguyen, T. D.; Nguyen, P. D.; Chau, V. V.; Dao, K. T. V.; Nguyen, H. T. N.; Kajimura, K.; Kumeda, Y.;

652

Bui, C. T.; Vien, M. Q.; Le, N. H.; Hirata, K.; Yamamoto, Y. Antibiotic residue monitoring results for

653

pork, chicken, and beef samples in Vietnam in 2012-2013. J. Agric. Food Chem. 2015, 63 (21),

654

5141-5145; DOI 10.1021/jf505254y.

655

(60) Li, Y. B.; Xu, J.; Wang, F.; Wang, B.; Liu, L. Q.; Hou, W. L.; Fan, H.; Tong, Y. Q.; Zhang, J.; Lu,

656

Z. X. Overprescribing in China, driven by financial incentives, results in very high use of antibiotics,

657

injections, and corticosteroids. Health Affair. 2012, 31 (5), 1075-1082; DOI 10.1377/hlthaff.2010.0965.

658

(61) Leung, H. W.; Jin, L.; Wei, S.; Tsui, M. M. P.; Zhou, B. S.; Jiao, L. P.; Cheung, P. C.; Chun, Y. K.;

659

Murphy, M. B.; Lam, P. K. S. Pharmaceuticals in tap water: Human health risk assessment and

660

proposed monitoring framework in China. Environ. Health Persp. 2013, 121 (7), 839-846; DOI

661

10.1289/ehp.1206244.

662

(62) Hamscher, G.; Pawelzick, H. T.; Sczesny, S.; Nau, H.; Hartung, J. Antibiotics in dust originating

663

from a pig-fattening farm: A new source of health hazard for farmers? Environ. Health Persp. 2003, 111

664

(13), 1590-1594; DOI 10.1289/ehp.6288.

665

(63) Callan, H. E.; Jenkins, R. E.; Maggs, J. L.; Lavergne, S. N.; Clarke, S. E.; Naisbitt, D. J.; Park, B.

666

K. Multiple adduction reactions of nitroso sulfamethoxazole with cysteinyl residues of peptides and

distribution

and

ecological risks.

Environ. Pollut.

32


2013,

174,

71-77;

DOI

Page 33 of 33


667

proteins: Implications for hapten formation. Chem. Res. Toxicol. 2009, 22 (5), 937-948; DOI

668

10.1021/tx900034r.

669

(64) Gill, H. J.; Hough, S. J.; Naisbitt, D. J.; Maggs, J. L.; Kitteringham, N. R.; Pirmohamed, M.; Park,

670

B. K. The relationship between the disposition and immunogenicity of sulfamethoxazole in the rat. J.

671

Pharmacol. Exp. Ther. 1997, 282 (2), 795-801.

672

(65) Komuro, M.; Higuchi, T.; Hirobe, M. Application of chemical cytochrome P-450 model systems

673

to studies on drug metabolism: VIII. Novel metabolism of carboxylic acids via oxidative

674

decarboxylation. Bioorg. Med. Chem. 1995, 3 (1), 55-65; DOI 10.1016/0968-0896(94)00141-O.

675

(66) Jung, J. Y.; Kim, Y.; Kim, J.; Jeong, D. H.; Choi, K. Environmental levels of ultraviolet light

676

potentiate the toxicity of sulfonamide antibiotics in Daphnia magna. Ecotoxicol. 2008, 17 (1), 37-45;

677

DOI 10.1007/s10646-007-0174-9.

678

(67) Gust, M.; Fortier, M.; Garric, J.; Fournier, M.; Gagné, F. Effects of short-term exposure to

679

environmentally relevant concentrations of different pharmaceutical mixtures on the immune response

680

of the pond snail Lymnaea stagnalis. Sci. Total Environ. 2013, 445-446, 210-218; DOI

681

10.1016/j.scitotenv.2012.12.057.

682

(68) Wang, H. L.; Che, B. G.; Duan, A. L.; Mao, J. W.; Dahlgren, R. A.; Zhang, M. H.; Zhang, H. Q.;

683

Zeng, A. B.; Wang, X. D. Toxicity evaluation of β-diketone antibiotics on the development of

684

embryo-larval zebrafish (Danio rerio). Environ. Toxicol, 2014, 29 (10), 1134-1146; DOI

685

10.1002/tox.21843.

33


Antibiotic Pollution in Marine Food Webs in ... - ACS Publications

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