Certain Perfluoroalkyl and Polyfluoroalkyl Substances Associated with

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Certain Perfluoroalkyl and Polyfluoroalkyl Substances Associated with Aqueous Film Forming Foam Are Widespread in Canadian Surface Waters Lisa Anne D'Agostino, and Scott Andrew Mabury Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.7b03994 • Publication Date (Web): 07 Nov 2017 Downloaded from http://pubs.acs.org on November 10, 2017

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Environmental Science & Technology

Certain Perfluoroalkyl and Polyfluoroalkyl Substances Associated with Aqueous Film Forming Foam Are Widespread in Canadian Surface Waters

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Lisa A. D’Agostino and Scott A. Mabury*

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Department of Chemistry, University of Toronto, 80 St George Street, Toronto, M5S

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3H6, ON, Canada

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[email protected]

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TOC Art

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ACS Paragon Plus Environment


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Abstract:

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The presence of perfluoroalkyl and polyfluoroalkyl substances (PFASs)

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commonly associated with aqueous film forming foams (AFFFs) at sites without known

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AFFF contamination is a largely unexplored area, which may reveal widespread

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environmental

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chromatography-tandem mass spectrometry (LC-MS/MS) screening for 23 classes of

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PFASs, followed by quantitative analysis was used to investigate surface waters from

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rural, urban, and AFFF-impacted sites in Canada. The PFASs detected included

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perfluorohexane sulfonamide (FHxSA), 6:2 fluorotelomer sulfonamide (FTSAm),

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fluorotelomer sulfonamide alkylbetaines (FTABs), fluorotelomer betaines (FTBs), 6:2

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fluorotelomer

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fluorotelomerthiohydroxyl ammonium sulfoxide (FTSHA-SO), 6:2 fluorotelomer

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sulfonamide alkylamine (FTAA) and C3 to C6 perfluoroalkane sulfonamido amphoterics.

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Detection of FHxSA in all urban and AFFF-impacted sites (0.04–19 ng/L) indicates the

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widespread presence of rarely considered perfluorohexane sulfonate (PFHxS) precursors

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in Canadian waters. FTABs and FTBs were especially abundant with up to 16–33 ng/L of

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6:2 FTAB in urban and AFFF-impacted water suggesting it may have additional

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applications, while FTBs were only in AFFF-impacted sites (qualitative; ΣFTBs 80

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ng/L). The distributions of PFASs moving downstream along the AFFF-impacted

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Welland River and between water and sediment suggested differences in the persistence

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of various AFFF components and enhanced sorption of long-chain fluorotelomer

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betaines. Total organofluorine combustion-ion chromatography (TOF-CIC) revealed that

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fluorotelomer betaines were a substantial portion of the organofluorine in some waters

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and 36–99.7% of the total organofluorine was not measured in the targeted analysis.

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Introduction

contaminants

requiring

mercaptoalkylamido

further

sulfonate

investigation.

sulfone

Sensitive

(FTSAS-SO2),

liquid

6:2

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Since 1999, aqueous film forming foams (AFFFs) used to fight liquid-fuelled fires

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have been implicated in local environmental contamination with perfluoroalkyl and

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polyfluoroalkyl substances (PFASs),1 particularly perfluoroalkyl carboxylates (PFCAs),1–

38

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perfluoroalkane sulfonates (PFSAs),2–7,9–11 and fluorotelomer sulfonic acids

2 Environment ACS Paragon Plus

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(FTSAs).4,12 Extremely high concentrations of PFASs have been associated with AFFF,

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including up to 2,210,000 ng/L of perfluorooctane sulfonate (PFOS) in Etobicoke Creek

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immediately after an AFFF release from Toronto Pearson Airport, Ontario in 20003 and

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up to 920,000 ng/L of perfluorohexane sulfonate (PFHxS) and 14,600,000 ng/L of 6:2

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FTSA in groundwater at Tyndall Air Force Base, Florida.4 Inputs of AFFF are also

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implicated in less extreme contamination of surface waters over longer time frames,

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including 290 ng/L of PFOS in Etobicoke Creek in 2009 near the AFFF release site from

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2000,7 and 45–64 ng/L of PFOS in Lake Niapenco downstream of Hamilton Airport,

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Ontario in 2010.5

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However, while PFOS is a major component of AFFFs prepared from

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electrochemical fluorination products and PFHxS may be found in these products at

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concentrations less than 15% of the PFOS concentration, most of these PFCAs, PFSAs,

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and FTSAs are not major PFASs in AFFFs relative to the primary PFAS active

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ingredients.14–16 In the past five years, over twenty additional classes of PFASs in AFFFs

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and commercial fluorinated surfactant concentrates17,18 and several additional degradation

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products of AFFF components14,19–21 have been identified. Reports of these recently

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identified AFFF components in the environment are limited and usually focus on sites

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known to have received substantial inputs of AFFF. Efforts to determine which of the

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AFFF-related PFASs can be detected at trace levels in surface waters without known

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AFFF impacts, such as urban and rural surface waters, are lacking. Only a study that

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estimated concentrations of fluorotelomer sulfonamide alkylamines (FTAAs) and

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fluorotelomer sulfonamide alkylbetaines (FTABs) in French river sediments at 0.055–

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13.5 ng/g in total investigated sites without significant known impacts from AFFF or

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AFFF component manufacturing.13

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The highly impacted sites that have been investigated in terms of novel AFFF-

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related PFASs include on and around military bases with long histories of AFFF use,14–19

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near airport and other firefighting training areas,19,20 the areas surrounding large oil fires

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that were fought with AFFF,19,21–23 and near a location of PFAS manufacturing for

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AFFF.24,25 These studies have determined that a variety of AFFF-related PFASs are

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present in environmental samples from these sites, with one or more congeners of the

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following fluorotelomer PFAS classes found in various environmental media:



FTAAs,17,19–23,25

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FTABs,17,19–25

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(FTSASs),14,21,23

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SO2s),18 FTSAS-related thioether/sulfone-linked carboxylic acids,18 fluorotelomer

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sulfonamides (FTSAms),21 fluorotelomerthiohydroxyl ammoniums (FTSHAs),21,23

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FTSHA-sulfoxides (FTSHA-SOs),21,23 demethyl-FTAAs,21 and fluorotelomer betaines

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(FTBs).21,23 Perfluoroalkane sulfonamido substances found in AFFFs or their likely

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degradation products have also been measured on highly AFFF impacted U.S. military

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bases, including C4–C6 perfluoroalkane sulfonamido alkylamine acids (FASAAAs),14

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C4–C6 perfluoroalkane sulfonamido alkylamines (FASAAms),14,15 and perfluorohexane

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sulfonamide (FHxSA).15,16 Many of these studies provided quantitative or semi-

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quantitative results for the novel AFFF-related PFASs in environmental matrices.14–

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17,19,21–25

FTSAS-sulfoxides

fluorotelomer

mercaptoalkylamido

(FTSAS-SOs),21,23

FTSAS-sulfones

sulfonates (FTSAS-

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However, by focusing investigations on highly AFFF impacted sites, previous

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studies were not able to address how widespread these contaminants are in the

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environment and whether the patterns in their detection and measured or estimated

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concentrations may suggest that they have applications outside of AFFF. For example,

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according to a patent, 6:2 FTAB may be used to coat ceramic surfaces to prevent

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deposits.26 In addition, a "betaine partially fluorinated surfactant," Capstone FS-50, is

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marketed for various applications, including cleaning and floor care, and has identical

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listed properties to Capstone 1157, which is marketed for use in AFFFs and is

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presumably a renamed Forafac 1157, which contained mostly 6:2 FTAB.20,27,28 Herein,

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by screening for 23 classes of PFASs with high sensitivity and quantifying, as possible,

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the AFFF-related PFASs found in surface water samples from rural, urban, and AFFF

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impacted locations in Canada, information about the environmental occurrence of PFASs

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typically considered AFFF-related is obtained. This shows which of these PFASs are

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relatively widespread in the environment due to their widespread use, high persistence,

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and/or environmental transport, which can help prioritize future research into the most

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environmentally relevant of these PFASs.

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In addition to the occurrence of AFFF-related PFASs in surface waters, the total

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organofluorine content of surface water extracts was determined by total organofluorine

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combustion ion chromatography (TOF-CIC). Comparison of results of this technique


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with targeted, quantitative analysis can show when a substantial amount of the total

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organofluorine is made up of additional components containing organofluorine that were

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not quantified. It has been applied to numerous matrices, including sediments,29 water,30

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and AFFF concentrates31 demonstrating that substantial amounts of organofluorine are

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not accounted for by targeted analysis. For example, the PFCAs and PFSAs measured in

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the acidic fraction of Lake Ontario surface sediment extracts accounted for only 2 to 44%

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of the total organofluorine.29 Using TOF-CIC, the contribution of novel AFFF-related

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PFASs to the total organofluorine in the surface water samples and the size of the

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remaining unknown fraction was investigated.

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Another important aspect of the behaviour of AFFF-related PFASs in surface

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water environments is sorption to sediment, because increased sorption can reduce the

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transport of PFASs downstream.32,33 Neither laboratory nor field determinations of the

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sorption behaviour of the novel AFFF-related PFASs are available. With this gap in

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knowledge, although the focus of this investigation is surface water, sediment samples

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were collected concomitantly with water samples from a subset of sites along the AFFF-

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impacted Welland River5,34 and at one nearby reference site. By comparing the PFAS

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profiles in corresponding sediment and water samples, preliminary insights into the

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sorption behaviour of the novel AFFF-related PFASs found in the Welland River were

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obtained. Where PFASs were quantified in both sediment and water at a site, field-

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derived sediment-water distribution coefficients (logKd) and organic carbon normalized

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distribution coefficients (logKOC) were determined. The determined field-derived logKOC

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values for PFAAs were compared to previous field derived values in river and coastal

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sediments,8,33,35 while field derived logKOC values and sediment concentrations of novel

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AFFF-related PFASs provide an indication of their sorption behaviour.

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Materials and Methods

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Materials

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6:2 FTAA, 6:2 FTAB, and 6:2 FTSAm were synthesized in house. As described

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in detail previously,36 6:2 fluorotelomer sulfonyl chloride (FTSO2Cl) was prepared by a

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one step synthesis from 6:2 fluorotelomer thiol, using KNO3 and SO2Cl2. The 6:2



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FTSO2Cl was reacted with N,N-dimethylamino-1-propylamine to form 6:2 FTAA or with

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NH3 in tetrahydrofuran to form 6:2 FTSAm. The 6:2 FTAA was used to prepare 6:2

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FTAB by refluxing it with sodium chloroacetate.36 A listing of the other solvents,

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chemicals, and standards used is in the Supporting Information (SI).

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Samples

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Welland River (sample points 1 to 9) are sequential sites along the AFFF-

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impacted Welland River. Because it was not possible to sample upstream of the former

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firefighting training site on the Welland River, since this site is in the headwaters, Big

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Creek (2 sample locations) and Welland River Reference, which are nearby rural sites not

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downstream of the former firefighting training site at Hamilton Airport, were sampled as

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rural reference sites. Water was collected in submerged 500 mL wide-mouth

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polypropylene bottles (Nalgene, Penfield, NY) on October 22, 2015. Water samples from

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two AFFF-impacted Arctic lakes, Resolute Lake and Meretta Lake, were collected in

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August 2012 and 2014 in 1 L polyethylene bottles (Kartell, Noviglio, Italy) by

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Environment and Climate Change Canada (ECCC) personnel. Rivers in Southern Ontario

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were sampled in conjunction with Ontario Ministry of the Environment and Climate

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Change (MOECC) sampling programs in 2015 with 500 mL polyethylene terephthalate

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jars used routinely for monitoring perfluoroalkyl acids (PFAAs). Of these rivers, Perch

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Creek, Thames River, Grand River, Humber River, and Don River are classified as urban;

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Etobicoke Creek has known prior AFFF-impacts. Additional rural samples were collected

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using 500 mL wide-mouth polypropylene bottles (Nalgene) from Little Rouge Creek in

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2016 and Lake of Bays in 2015 some of which were used for matrix spike and recovery.

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Samples were refrigerated at 4°C prior to extraction. Sampling dates and locations are in

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SI Figure S1 and Table S1. Sediment samples were obtained in 500 mL wide-mouth

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polypropylene bottles (Nalgene) from four Welland River and one Big Creek site at the

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same time and place as water samples. Sediments were frozen at -20°C and lyophilized

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prior to extraction.

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Because the Welland River upstream of the Binbrook Dam flows very slowly,

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these sediments were likely at or near equilibrium with the surrounding waters. The

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month of October 2015, prior to sampling, was relatively dry and water levels in the


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reservoir (Lake Niapenco) remained below the holding level of 650.5 feet throughout

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September and October 2015, prior to the sampling.37 Therefore, the residence time of

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water likely was quite long prior to sampling and the water was relatively stagnant.

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Observations at the time of sampling support this assessment as the water was not

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observed to flow and the flow out of the Binbrook Dam was a trickle.

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Water Extraction

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Water samples were extracted in the Advanced Laboratory for Fluorinated and

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Other New Substances in the Environment (ALFONSE) clean laboratory (Class 100A)

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using Oasis WAX solid phase extraction (SPE) cartridges (6 mL, 200 mg, 30 µm; Waters,

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Milford, MA). The extraction protocol used to extract the approximately 500 mL water

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samples was a modification of existing methods38 and is described fully in the SI. The

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cartridges were eluted with methanol (fraction containing neutrals, zwitterions, bases, and

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cations) followed by 0.1% NH4OH in methanol (fraction containing acids).

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Sediment Extraction

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Approximately 0.5 g subsamples of lyophilized sediment were weighed into

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polypropylene centrifuge tubes and extracted using 0.1% NH4OH in methanol based on

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Houtz et al.15 with clean-up using 1mL/100mg Supelclean ENVI-Carb SPE cartridges

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(Supelco, Bellefonte, PA) in the ALFONSE clean laboratory. The full extraction

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procedure and the method for determination of organic carbon content are described in

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the SI.

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LC-MS/MS

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Analysis of sample extracts for PFASs by LC-MS/MS was performed using an

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Acquity UPLC–Xevo TQ-S system (Waters, Milford, MA). An Acquity UPLC BEH C18

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column (1.7 µm, 2.1 mm×75 mm) and a mobile phase gradient of 10 mM aqueous

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ammonium acetate and methanol were used. The Xevo TQ-S was operated in positive

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and negative electrospray (ESI) mode. Further LC-MS/MS method details are in the SI.

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Transitions for AFFF components (SI Table S2) were optimized by infusing dilutions of



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suitable AFFF extracts prepared previously.39 Screening LC-MS/MS runs were used to

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determine which PFASs may be present in sample extracts and included transitions for

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additional AFFF-components that were never detected.

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The suite of PFAAs analyzed is limited to PFOS or PFOA and shorter in order to

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focus on those PFAAs most associated with AFFFs, which generally contain primarily

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PFOS and C6 or C8 perfluoroalkane sulfonamido substances or 6:2 fluorotelomer

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surfactants. The shortest PFCA analyzed was PFPeA because PFBA was poorly retained

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on the reverse phase column and suffered from background contamination.

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TOF-CIC

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Surface water extracts from each sampling location were analyzed by total

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organofluorine-combustion ion chromatography (TOF-CIC) using previously published

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procedures.31,40 The acid extracts were also analyzed for inorganic fluoride by combining

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100 µL of extract with 6.5 mL of deionized water and directly analyzing by ion

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chromatography using the TOF-CIC system.

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Quality Assurance of Data

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Analyses by LC-MS/MS were completed in at least duplicate (n=2–4).

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Quantification of PFASs was performed in several ways depending on the availability of

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standards.

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perfluoroheptanoate (PFHpA), perfluorooctanoate (PFOA), PFHxS, PFOS, 4:2 FTSA,

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6:2 FTSA, 8:2 FTSA, N-ethyl perfluorooctane sulfonamido acetic acid (EtFOSAA), and

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perfluorooctane sulfonamide (FOSA) were quantified using internal calibration with

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isotopically labeled standards. Perfluorobutane sulfonate (PFBS), perfluoropentane

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sulfonate (PFPeS), perfluoroheptane sulfonate (PFHpS), FHxSA, and 6:2 FTSAm were

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quantified using structurally related surrogate mass-labeled internal standards: mass-

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labeled PFHxS for PFBS and PFPeS, mass-labeled PFOS for PFHpS, and mass-labeled

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FOSA for FHxSA and 6:2 FTSAm. Two multiple reaction monitoring transitions were

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used to confirm detection of all PFASs, except PFPeA, PFHxA, EtFOSAA, FHxSA, and

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FOSA, where a single transition was used. For 6:2 FTAB and 6:2 FTAA, matrix matched

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calibration was used along with mass-labeled FOSA internal standard. The data are

Perfluoropentanoate

(PFPeA),

perfluorohexanoate


(PFHxA),

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considered quantitative for the PFASs analyzed as described above with commercial

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analytical standards or standards prepared from neat materials. Estimation of the

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concentrations for qualitative analytes (novel PFASs) for which isolated standards were

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unavailable was performed by using matrix matched calibration curves for 6:2 FTSA for

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sulfonic acid-containing compounds, EtFOSAA for carboxylic acid compounds, 6:2

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FTAA for amine or quaternary ammonium compounds, and 6:2 FTAB for amphoteric

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compounds. To confirm the detection of qualitative AFFF components, two MS/MS

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transitions were used and retention times were matched to AFFF extracts containing

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those components from a previous study.39

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The calibration curves had a minimum of five points and were usually constructed

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from 0.02 ng/mL to 10 ng/mL in the vial (range: 0.006–0.03 ng/mL to 10 ng/mL). This

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corresponds to 0.08 ng/L to 40 ng/L in a 500 mL water sample and 0.08 ng/g to 40 ng/g

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in a 0.5g sediment sample. A quality control sample spiked at 1 ng/mL in the vial with all

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the PFASs being quantified was analyzed in duplicate during each analysis and analyses

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were accepted if the concentrations determined were within 20%. Where the

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concentration of a PFAS was above the highest calibration point in a sample, that sample

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was rerun with a suitable dilution factor. The limit of detection (LOD) was defined as the

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concentration giving a signal-to-noise ratio of 3 in the sample matrix and the limit of

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quantitation (LOQ) was the concentration giving a signal-to-noise ratio of 10 in the

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sample matrix. Matrix LODs and LOQs are given in SI Table S4. During each water

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extraction, two cartridge blanks were subjected to all portions of the extraction procedure

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except sample loading to evaluate contamination of the extraction solvents and contained

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no PFASs above the LOD. Two field blanks of deionized water that were opened to air

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during the collection of one Welland River sample were also extracted and found to

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contain no PFASs above the LOD. For the sediment extraction, two solvent blanks

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containing no sediment were extracted along with the sediments and contained no PFASs

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above the LOD. Inter-day duplicate extractions were performed on Welland River 1, 2, 5,

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6, 7, and 9; Welland River Reference; and Big Creek 1 water samples, while intra-day

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duplicate extractions were performed on all sediments and Little Rouge Creek water.

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Recoveries for water extraction were determined by spiking surface water

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samples (~500 mL) from relatively clean environments (n=5) with 2.5 ng of FTSAs,



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EtFOSAA, FHxSA, FOSA, 6:2 FTSAm, 6:2 FTAA, and 6:2 FTAB and 25 ng of PFCAs

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and PFSAs in methanol, swirling to mix, and extracting the following day. The recoveries

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were 71–96% for all PFASs, including recoveries of 88±24% for 6:2 FTAB (zwitterionic)

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and 71±12% for 6:2 FTAA (cationic at surface water pH; SI Table S3). Recoveries from

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freeze dried sediment were determined by spiking sediment from Big Creek 1 (~0.5 g,

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n=4) with 1 ng each of PFCAs, FTSAs, EtFOSAA, FOSA, FHxSA, 6:2 FTSAm, 6:2

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FTAB, and 6:2 FTAA, and 5 ng each of PFSAs in 100 µL of methanol, vortexing to mix,

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and extracting the sediment the following day. Recoveries from sediment were 76–94%

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for the PFASs, except 6:2 FTAB (31±2%; SI Table S3). Sediment concentrations are

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reported without correction.

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Statistical Analysis

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Statistical comparison of the concentrations of PFASs found at rural (n=5), urban

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(n=5), and AFFF-impacted (n=14) sampling sites were performed using the two-tailed

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Mann-Whitney U test. For the statistical analysis, values determined between LOQ and

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LOD were used as determined from the calibration and concentrations below the

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detection limit were substituted with half the LOD. The Mann-Whitney U test was only

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used for PFASs detected in at least 21 out of 24 samples or 17 out of 19 urban and AFFF

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impacted samples to limit the impact of non-detects. The threshold for statistical

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significance used was p

Certain Perfluoroalkyl and Polyfluoroalkyl Substances Associated with

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