Genetically Incorporating Two Distinct Post-translational Modifications

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Genetically Incorporating Two Distinct Post-translational Modifications into One Protein Simultaneously Sumana Venkat, Jourdan Sturges, Alleigh Stahman, Caroline Gregory, Qinglei Gan, and Chenguang Fan ACS Synth. Biol., Just Accepted Manuscript • DOI: 10.1021/acssynbio.7b00408 • Publication Date (Web): 04 Jan 2018 Downloaded from http://pubs.acs.org on January 6, 2018

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ACS Synthetic Biology

Genetically Incorporating Two Distinct Post-translational Modifications into One Protein Simultaneously

Sumana Venkat1,2, Jourdan Sturges3, Alleigh Stahman1, Caroline Gregory3, Qinglei Gan1, Chenguang Fan*,1,2

1

Department of Chemistry and Biochemistry,

2

Cell and Molecular Biology Program, and

3

Department of Biological Sciences, University of Arkansas, Fayetteville, AR, 72701

* Corresponding author: Chenguang Fan; 119 Chemistry Building, University of Arkansas, Fayetteville, AR, 72701; Telephone: 479-575-4653; E-mail: [email protected]

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ABSTRACT Post-translational modifications (PTMs) play important roles in regulating a variety of biological processes. To facilitate PTM studies, the genetic code expansion strategy has been utilized to co-translationally incorporate individual PTMs such as acetylation and phosphorylation into proteins at specific sites. However, recent studies have demonstrated that PTMs actually work together to regulate protein functions and structures. Thus, simultaneous incorporation of multiple distinct PTMs into one protein is highly desirable. In this study, we utilized the genetic incorporation systems of phosphoserine and acetyllysine to install both phosphorylation and acetylation into target proteins simultaneously in Escherichia coli. And we used this system to study the effect of coexisting acetylation and phosphorylation on malate dehydrogenase, demonstrating a practical application of this system in biochemical studies. Furthermore, we tested the mutual orthogonality of three widely-used genetic incorporation systems, indicating the possibility of incorporating three distinct PTMs into one protein simultaneously.

Key word: genetic code expansion, post-translational modification, phosphorylation, acetylation, noncanonical amino acid.


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Post-translational modifications (PTMs) play key roles in a wide range of biological processes across three domains of life.1 Proteomic studies have identified hundreds of types of PTMs in nature,

such as

phosphorylation,

acetylation,

methylation, ubiquitylation, and sulfation.2,

3

glycosylation,

amidation,

hydroxylation,

However, limited studies have been reported to

confirm and further characterize these mass spectrometry (MS)-identified PTM events. One challenge is to generate homogenously modified proteins at specific sites. For this purpose, the genetic code expansion strategy has been applied to site-specifically incorporate a number of PTMs into proteins,4 including lysine acetylation 5-7 and serine phosphorylation.8-11 Different from PTMs which occur after translation, the genetic code expansion approach is able to cotranslationally incorporate modified amino acids directly to produce homogeneously modified proteins at desired positions without knowing specific modifying enzymes such as kinases or acetyltransferases. Although the genetic code expansion approach provide powerful tools to study biology,12-16 previous studies could only incorporate one type of PTM into proteins individually. However, it is known now that proteins commonly have multiple PTMs simultaneously, and different types of PTMs interact with each other to affect protein structures, functions, and interactions.17, 18 Thus, it is highly desirable to develop genetic incorporation systems to install multiple distinct PTMs in one protein simultaneously. Scientists have been able to incorporate two distinct noncanonical amino acids (ncAAs) into one protein at different positions by using mutually orthogonal translation systems (OTSs), towards two different stop codons 19-24 or one stop codon together with one four-base codon.25-27 In this study, we expanded this strategy to simultaneously incorporate two of the most abundant PTMs in nature,28 phosphoserine (Sep) and acetyllysine (AcK), into target proteins by using E. coli cells. We also demonstrated a practical application of this dual-PTM incorporation system in biochemical characterization of enzymes. Furthermore, we demonstrated that three widely used OTSs in E. coil: the Methanocaldococcus jannaschii tyrosyl-tRNA synthetase (mjTyrRS)



system, the Methanosarcina species pyrrolysyl-tRNA synthetase (PylRS) system, and the Methanococcus maripaludis phosphoseryl-tRNA synthetase (SepRS) system are mutually orthogonal to each other, providing a solid base for installing three distinct PTMs simultaneously into one protein.

Results and Discussion Codon selection Three stop codons UAG, UGA, and UAA, as well as several four-base codons like AGGA have been used as signals to incorporate ncAAs by the genetic code expansion strategy.29-32 Thus, we firstly compared the incorporation efficiency and accuracy of different codons by the superfolder green fluorescent protein (sfGFP) reporter system (Figure 1). Because of the low selectivity of PylRS toward the tRNAPyl anticodon,33, 34 we used the recently optimized AcKRS which was derived from PylRS by phage-assisted protein evolution35 and the optimized tRNAPyl for better EF-Tu binding36 as the testing OTS towards UAG, UGA, UAA, and AGGA codons, individually. It is known that ncAA incorporation efficiency varies at different positions even in the same protein.37 So we chose three different permissive sites in sfGFP (97, 151, and 182) for this experiment.37 The corresponding position in the gene of sfGFP was mutated to TAG, TGA, TAA, or AGGA, individually. The anticodon of tRNAPyl was correspondingly mutated to CUA, UCA, UUA, or UCCU, respectively. Here, we did not introduce the orthogonal ribosome for the quadruplet reading, though it could enhance ncAA incorporation.32 Firstly, the suppressor tRNA needs to be optimized for individual codons.26 And the efficiency of frameshift suppression might be another concern.20 The sfGFP readthrough assay showed that UGA suppression gave highest readings, but it also had high backgrounds if no AcK was supplemented in the medium, possibly due to near cognate suppression.38, 39 The four-base codon AGGA had the lowest efficiency and the highest


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backgrounds if no AcK added in the medium which might be caused by AGG decoding as Arg and then +1 frame shifting,39 So we chose UAG and UAA codons for later experiments which could provide high purity of ncAA incorporation without losing efficiency significantly.

Orthogonality among OTSs It has been demonstrated that OTSs derived from the mjTyrRS/tRNATyr pair is orthogonal to OTSs engineered from the PylRS/tRNAPyl pair.19, 24 However, the orthogonality of Sep-OTS to these two systems has not been reported before. For this purpose, we used the sfGFP reporter system to test different combinations of AARSs and tRNAs. The permissive position 151 in sfGFP was selected for UAG or UAA codon suppression tests. AcK-OTS was chosen as a representative of the PylRS system, while pTyr-OTS was selected for the mjTyrRS system. tRNAs with CUA or UUA anticodon were tested for UAG or UAA suppression, respectively (Figure 2). Results showed that all the AARSs tested could only recognize their cognate tRNA suppressors. The change of tRNA anticodon from CUA to UUA decreased the efficiencies of both Sep-OTS and pTyr-OTS significantly. This is probably because both SepRS and mjTyrRS bind to anticodon regions of their cognate tRNAs, and they were engineered for optimal binding with the CUA anticodon originally.40, 41 On the other hand, the change of tRNAPyl anticodon did not affect the AcK-OTS efficiency as expected. Thus, we chose the UAG codon as the signal to incorporate Sep and the UAA codon for AcK incorporation in later experiments. The mutual orthogonality of these three widely used OTSs indicates the possibility of incorporating three different PTMs into one protein at the same time. For example, phosphotyrosine can be incorporated by systems originally from the mjTyrRS system,42, 43 while phosphothreonine has been incorporated by the SepRS system derivative.44 So it is possible to generate a protein with tyrosine phosphorylation, threonine or serine phosphorylation, and lysine acetylation at controlled positions simultaneously. In this case, the UAG codon could be used for phosphoserine or phosphothreonine incorporation by the SepRS system or its



derivative. The UAA codon could be used for phosphotyrosine incorporation since the mjTyrRS system has been used to readthrough the UAA codon.24 Due to the flexibility of codon recognition of tRNAPyl, either the UGA codon or a four-base codon could be selected for acetyllysine incorporation.

Simultaneously installing serine phosphorylation and lysine acetylation in the sfGFP Firstly, we used the sfGFP as a validation reporter. Two permissive positions S2 and K101 were selected to incorporate Sep and AcK, respectively. The serine codon at position 2 was mutated to UAG, and the Sep-OTS was cloned into a pKD plasmid with the pBR322 origin and kanamycin resistance. The lysine codon at position 101 was mutated to UAA. Correspondingly, the anticodon of tRNAPyl was mutated to UUA, and the AcK-OTS was cloned in a pTech plasmid with the p15A origin and chloramphenicol resistance. The gene of sfGFP or its variant was cloned into a pCDF plasmid with the CloDF13 origin and streptomycin resistance. All the three plasmids were co-transformed into BL21(DE3) cells. The expression of sfGFP and its variant was analyzed by SDS-PAGE (Figure 3a). The yield of the sfGFP variant with both Sep and AcK incorporated was 52 mg per liter culture, while the yield of the wild-type sfGFP is 350 mg per liter culture under the same growth condition. If no AcK was supplemented in the medium, there was no detectable full-length sfGFP on the gel. Interestingly, when only AcK but no Sep was supplemented in the medium, there was a clear band of full-length sfGFP on the gel (Lane 4 in Figure 3a). There are three possible reasons for this unexpected result. Firstly, Sep is an intermediate in the serine biosynthesis pathway,45 so SepRS could charge tRNASep with free Sep in cells to read through the UAG codon. Secondly, due to the G:U wobble pairing, tRNAPyl with the UUA anticodon could also suppress the UAG codon partially.46 This was also found in the recent publication which used the cell-free translation system to incorporate two distinct ncAAs.24 Thirdly, the UAG codon could also be recognized by near-cognate tRNAs for the incorporation of canonical amino acids.38, 39 While 2 mM Sep was added to the medium, the


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expression of the sfGFP variant increased significantly. The simultaneous incorporation of Sep and AcK was confirmed with full-length MS analyses (Figure 3b, S1-2). The difference of molecular weights between the wild-type sfGFP and Sep/AcK-containing sfGFP variant was 122 Da, which equals to the expected value with both phosphorylation (80 Da) and acetylation (42 Da). The positions of Sep and AcK incorporation were confirmed by LC-MS/MS (Figure S34). We did not detect the incorporation of AcK in the UAG codon position by both full-length MS and LC-MS/MS, which was consistent with the previous study showing that the UAA suppressor did not readthrough the UAG codon when both ncAAs were supplemented into the medium.24

Simultaneously incorporating AcK and Sep into malate dehydrogenase (MDH) To demonstrate a practical application of our system in biochemical studies, the MDH in the citrate acid cycle was selected. Previous proteomic studies have shown that E. coli MDH has lysine acetylation at position 140 and serine phosphorylation at position 280.47,

48

By site-

specifically incorporating AcK or Sep into MDH individually, we demonstrated that lysine acetylation at position 140 could increase the enzyme activity,49 while serine phosphorylation at position 280 impaired the enzyme activity.50 To explore the overall effect of these two PTMs, we used our dual-PTM incorporation system to produce simultaneously modified MDH. The serine codon at position 280 was mutated to UAG, and the lysine codon at position 140 was mutated to UAA. The same plasmid systems in sfGFP expression were used. The expression of MDH and its variant was analyzed by SDS-PAGE (Figure 4a). The yield of the MDH variant with both Sep and AcK incorporated was 6 mg per liter culture, while the yield of wild-type MDH is 35 mg per liter culture under the same growth condition. Again, if no AcK was supplemented in the medium, there was no detectable full-length MDH. When only AcK was supplemented in the medium, there was a faint band of MDH probably due to endogenous Sep in cells and wobble pairing of the UAG codon with the UUA anticodon in the UAA suppressor. Addition of 2 mM Sep in the medium could increase MDH expression. The simultaneous incorporation of Sep and AcK



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was confirmed by both western blotting (Figure 4b) and full-length MS analyses (Figure 4c, S56). The difference of molecular weights between the wild-type and Sep/AcK-containing MDH was 121 Da, which is close to the expected value with both phosphorylation (80 Da) and acetylation (42 Da). The positions of Sep and AcK incorporation were confirmed by LC-MS/MS (Figure S7-8). Again, we did not detect AcK incorporation at the UAG codon position when both AcK and Sep were added into the medium. In both sfGFP and MDH expression experiments, the yield of doubly modified proteins was about 15% of that of wild-type proteins (Figure 3a and 4a). Generally, the more stop codons were used, the lower efficiency of ncAA incorporation should be observed due to the competition with release factors, and the more mis-incorporation of canonical amino acids might also be detected because of near cognate suppression. Thus, both the protein yield and fidelity would be even lower when incorporating three different PTMs at the same time. To overcome these issues, the plasmid system should be optimized to increase the expression of different OTSs22, individual OTSs should be further engineered for higher efficiencies

35, 51

, and special

strains could be used such as the strain with UAG codon reassignment which has the gene of the release factor-1 removed and all TAG codons in the genome replaced with TAA codons to eliminate the competition for the UAG codon with the release factor-1.51

Studying effects of acetylation and phosphorylation on MDH Then, we measured the enzyme activities of wild-type, AcK only-containing, Sep only-containing, and AcK/Sep-containing MDHs (Figure 5a). Consistent with our previous studies, lysine acetylation at position 140 increased the enzyme activity by 3.5-fold, while serine phosphorylation at position 280 decreased the enzyme activity to only 30% of that of the wildtype enzyme. Interestingly, the combination of 140-AcK and 280-Sep had a similar activity with the wild-type enzyme, indicating that two PTMs work separately to affect MDH activity. We mapped these two residues onto the crystal structure of the MDH tetramer.52 Both residues are


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not at the active site but at the dimer-dimer interface (Figure 5B and 5C). From a zoom-in view of amino acids in the interface, K140 and S280 are at one side, and four hydrophobic residues (V189, P190, G191, and V192) are at the other side (Figure 5D). Our enzyme activity assays showed that acetylation (removal of the charge) of K140 increased the activity, while phosphorylation (addition of the charge) of S280 decreased the activity. So we proposed that better hydrophobic interaction in the dimer-dimer interface might increase the MDH activity. To test our assumption, we mutated S280 to alanine, valine, or leucine, separately. We also mutated K140 to glutamine which is traditionally used as a mimic of lysine acetylation. Then we measured activities of these MDH variants (Figure 5A). As expected, the S280V variant facilitated the reaction. However, the S280A variant had only a similar activity with the wild-type MDH which might be due to its smaller side chain, and the steric hindrance of leucine probably caused the impairment. Unexpectedly, the K140Q variant only had a slightly improved activity, indicating that glutamine may not always be an ideal mimic for lysine acetylation, while sitespecific incorporation of acetyllysine could provide direct evidence of the acetylation impacts on proteins.

Methods Chemicals and materials. Chemicals were purchased from Sigma-Aldrich (St. Louis, MO), BDH Chemicals (Radnor, PA) or CHEM-IMPEX (Wood Dale, IL). Primers were ordered from Integrated DNA Technologies (Coralville, IA). DNA sequencing were performed by Eurofins Scientific (Louisville, KY). Plasmid purification kits were purchased from Qiagen (Hilden, Germany). Cloning enzymes and site-directed mutagenesis kits were purchased from New England Biolabs (Ipswich, MA). Antibodies were purchased from Cell Signaling Technology (Danvers, MA).



Plasmid construction. DH5α cells were used for cloning purposes. The genes of aminoacyltRNA synthetases (AARSs) and their cognate tRNAs as well as target proteins were amplified from laboratory inventory, and inserted into corresponding vectors by DNA assembly. Mutations in genes of AARSs, tRNAs, and target proteins were made by site-directed mutagenesis. In the Sep-OTS, sequences of SepRS, EF-Sep, and tRNAsep were from the previous study.40 In the AcK-OTS, the sequence of optimized AcKRS is from a recent study,35 and the optimized tRNAPyl was from our previous study.36 In the phosphotyrosine (pTyr)-OTS, the sequence of pTyrRS was from our recent study,42 and the optimized tRNATyr was from the previous study.53 Strains used in this study are listed in Table S1.

The sfGFP readthrough assay. The protocol was modified slightly from the previous study.54 BL21(DE3) cells were used to express sfGFP and its variants. Strains harboring genes of sfGFP or its variants under the control of the lac promoter in the pCDF plasmid and OTSs in the pTech plasmid (AARSs are constitutively expressed by the lpp promoter, while tRNAs are constitutively expressed by the ProK promoter) were inoculated into 2 mL LB media, and grown at 37 °C overnight. For the pTyr-OTS testing, the specific phosphatase-removal strain and plasmids were used.42 The overnight culture was diluted with fresh LB medium to an absorbance of 0.15 at 600 nm, supplemented with corresponding ncAAs (2 mM Sep, 5 mM AcK, or 10 mM pTyr), 100 µg mL-1 streptomycin, and 50 µg mL-1 chloramphenicol. Then 0.05 mM Isopropyl-β-Dthiogalactoside (IPTG) was added to induce the expression of sfGFP or its variants. 200 µL culture of each strain was transferred to a 96-well clear bottom black microplate. Cells were grown at 37 °C with shaking. Fluorescence intensity (excitation 485 nm, emission 528 nm, bandwidths 20 nm) and optical density (absorbance at 600 nm) were read by the microplatereader.


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Protein expression, purification and characterization. Protocols were modified slightly from the previous study.55 The genes of target proteins were cloned into the pCDF-1b plasmid with a C-terminal His6-tag, and co-transformed into BL21(DE3) cells with corresponding vectors harboring different OTSs. The expression strain was grown in 1 L of LB medium supplemented with 100 µg mL-1 streptomycin, 50 µg mL-1 chloramphenicol, and 50 µg mL-1 kanamycin if needed at 37 °C to an absorbance of 0.6–0.8 at 600 nm. Then 0.05 mM IPTG, 2 mM Sep, 5 mM AcK, and 20 mM nicotinamine (NAM, a deacetylase inhibitor) were supplemented to the medium. Cells were incubated at 25 °C for an additional 16 h, and harvested by centrifugation at 3,000 × g for 15 min at 4 °C. The pellet was suspended in 15 mL lysis buffer (50 mM Na2HPO4, pH 8.0, 300 mM NaCl, 20 mM imidazole, 20 mM NAM, and 150 µL 100X phosphatase inhibitor cocktail [Sigma-Aldrich]), and broken by sonication. The crude extract was centrifuged at 18,000 × g for 20 min at 4 °C. The soluble fraction was loaded onto a column containing 3 mL Ni-NTA resin (Qiagen, Hilden, Germany) previously equilibrated with 20 mL lysis buffer. The column was washed with 20 mL washing buffer (50 mM Na2HPO4, pH 8.0, 300 mM NaCl, and 50 mM imidazole), and eluted with 2 mL elution buffer (50 mM Na2HPO4, pH 8.0, 300 mM NaCl, and 150 mM imidazole). The purified proteins were fractionated by SDS-PAGE with 4-20% gradient gels, and stained with Coomassie blue. For western blotting, the unstained SDS-PAGE gel was transferred onto a PVDF membrane, blocked by 20 mL blocking buffer (5% bovine serum albumin in Tris-buffered saline, 0.1% Tween 20) for 2 h, and then soaked with 1:1000 diluted horseradish peroxidase (HRP)-conjugated antibodies overnight at 4°C with gentle shaking. The membrane was prepared for detection by using Pierce ECL Western Blotting substrates (Thermo Scientific, Waltham, MA).

Mass spectrometry (MS) analyses. The full-length MS analyses were performed by the University of Arkansas Statewide Mass Spectrometry Facility. Purified proteins were dialyzed with pure water, diluted to 0.05 mg mL-1, and analyzed by a Bruker UltraflexII TOF-TOF with a



MALDI ionization source. The laser was an Azura Laser AG diode pumped solid state laser (Nd:YAG, 355nm). The matrix used was a saturated solution of sinipinic acid in water/acetonitrile. Spectra were obtained in the positive ion, linear mode. The LC-MS/MS analyses were performed by Yale University Keck Proteomics Facility. The protocol was the same as the previous study.56 Proteins were digested by trypsin with a standard in-gel digestion protocol, and analyzed by LC-MS/MS on an LTQ Orbitrap XL equipped with a nanoACQUITY UPLC system. The Mascot search algorithm was used to search for the appropriate noncanonical substitution (Matrix Science, Boston, MA).

The malate dehydrogenase (MDH) activity assay. The protocol was modified slightly from the previous study.49 10 ng purified MDH or its variants was added to a 200 µL reaction mixture, following the instruction provided by the malate dehydrogenase assay kit (BioAssay Systems, Hayward, CA). The reaction mixture was added to a 96-well microplate, and the absorbance at 565 nm was read by a microplate reader.

Author Information Corresponding Author: *E-mail: [email protected] Author contribution: SV and CF designed experiments, analyzed data, and wrote the manuscript. All authors performed experiments and edited the manuscript. Notes: All authors declare no competing financial interest.

Acknowledgements This work was supported by a grant from National Institutes of Health (National Institute of Allergy and Infectious Diseases; AI119813), the start-up fund from University of Arkansas, and the grant from Arkansas Biosciences Institute.


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Supporting Information The list of strains and plasmids used in this work and mass spectra of modified proteins were supplied as Supporting Information.

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27. Neumann, H., Wang, K., Davis, L., Garcia-Alai, M., and Chin, J. W. (2010) Encoding multiple unnatural amino acids via evolution of a quadruplet-decoding ribosome, Nature 464, 441-444. 28. Khoury, G. A., Baliban, R. C., and Floudas, C. A. (2011) Proteome-wide post-translational modification statistics: frequency analysis and curation of the swiss-prot database, Sci Rep 1. 29. Chin, J. W. (2017) Expanding and reprogramming the genetic code, Nature 550, 53-60. 30. Liu, C. C., and Schultz, P. G. (2010) Adding new chemistries to the genetic code, Annu Rev Biochem 79, 413-444. 31. Mukai, T., Lajoie, M. J., Englert, M., and Soll, D. (2017) Rewriting the Genetic Code, Annu Rev Microbiol 71, 557-577. 32. Wang, K., Schmied, W. H., and Chin, J. W. (2012) Reprogramming the genetic code: from triplet to quadruplet codes, Angew Chem Int Ed Engl 51, 2288-2297. 33. Wan, W., Tharp, J. M., and Liu, W. R. (2014) Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool, Biochim Biophys Acta 1844, 10591070. 34. Ambrogelly, A., Gundllapalli, S., Herring, S., Polycarpo, C., Frauer, C., and Soll, D. (2007) Pyrrolysine is not hardwired for cotranslational insertion at UAG codons, Proc Natl Acad Sci U S A 104, 3141-3146. 35. Bryson, D. I., Fan, C., Guo, L. T., Miller, C., Soll, D., and Liu, D. R. (2017) Continuous directed

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37. Albayrak, C., and Swartz, J. R. (2013) Cell-free co-production of an orthogonal transfer RNA activates efficient site-specific non-natural amino acid incorporation, Nucleic Acids Res 41, 5949-5963. 38. Gan, Q., and Fan, C. (2016) Increasing the fidelity of noncanonical amino acid incorporation in cell-free protein synthesis, Biochim Biophys Acta 1861, 3047-3052. 39. O'Donoghue, P., Prat, L., Heinemann, I. U., Ling, J., Odoi, K., Liu, W. R., and Soll, D. (2012) Near-cognate suppression of amber, opal and quadruplet codons competes with aminoacyltRNAPyl for genetic code expansion, FEBS Lett 586, 3931-3937. 40. Lee, S., Oh, S., Yang, A., Kim, J., Soll, D., Lee, D., and Park, H. S. (2013) A facile strategy for selective incorporation of phosphoserine into histones, Angew Chem Int Ed Engl 52, 5771-5775. 41. Gan, R., Perez, J. G., Carlson, E. D., Ntai, I., Isaacs, F. J., Kelleher, N. L., and Jewett, M. C. (2017) Translation system engineering in Escherichia coli enhances non-canonical amino acid incorporation into proteins, Biotechnol Bioeng 114, 1074-1086. 42. Fan, C., Ip, K., and Soll, D. (2016) Expanding the genetic code of Escherichia coli with phosphotyrosine, FEBS Lett 590, 3040-3047. 43. Luo, X., Fu, G., Wang, R. E., Zhu, X., Zambaldo, C., Liu, R., Liu, T., Lyu, X., Du, J., Xuan, W., Yao, A., Reed, S. A., Kang, M., Zhang, Y., Guo, H., Huang, C., Yang, P. Y., Wilson, I. A., Schultz, P. G., and Wang, F. (2017) Genetically encoding phosphotyrosine and its nonhydrolyzable analog in bacteria, Nat Chem Biol 13, 845-849. 44. Zhang, M. S., Brunner, S. F., Huguenin-Dezot, N., Liang, A. D., Schmied, W. H., Rogerson, D. T., and Chin, J. W. (2017) Biosynthesis and genetic encoding of phosphothreonine through parallel selection and deep sequencing, Nat Methods 14, 729-736. 45. Pizer, L. I. (1963) The Pathway and Control of Serine Biosynthesis in Escherichia Coli, J Biol Chem 238, 3934-3944.



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Figure 1

Normalized fluorescence

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18000 5 mM AcK 15000

No AcK

12000 9000 6000 3000 0

Figure 1. The sfGFP readthrough assay towards various codons at different positions. Normalized fluorescence intensities were calculated from fluorescence readings at 12 h divided by absorbance at 600 nm. Mean values and standard deviations were calculated from three replicates.


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Figure 2

UAG suppression 6000

Normalized fluorescence

1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60


4000 2000 0 1

2

3

4

5

6

7

8

9

7

8

9

UAA suppression 6000 4000 2000 0 1

2

3

4

5

6

Figure 2. The orthogonality of different OTSs towards UAG or UAA codon. Lane 1: AcKRS/tRNAPyl; Lane 2: AcKRS/tRNATyr; Lane 3: AcKRS/tRNASep; Lane 4: SepRSEFSep/tRNASep; Lane 5: SepRS-EFSep/tRNAPyl; Lane 6: SepRS-EFSep/tRNATyr; Lane 7: pTyrRS-EFpY/tRNATyr; Lane 8: pTyrRS-EF-pY/tRNAPyl; Lane 9: pTyrRS-EF-pY/tRNASep. The anticodons of tRNAs were mutated to UUA for UAA codon suppression. For ncAAs, 5 mM AcK, 2 mM Sep, or 10 mM pTyr was added in the medium, respectively. Normalized fluorescence intensities were calculated from fluorescence readings at 12 h divided by absorbance at 600 nm. Mean values and standard deviations were calculated from three replicates.



Figure 3

Figure 3. Expression of Sep/AcK-containing sfGFP. A) The Coomassie blue-stained SDSPAGE gel of purified full-length sfGFP and its variant. The same volumes of elution fractions were loaded on the SDS-PAGE gel. B) MALDI-TOF mass spectra of purified full-length sfGFP (upper) and its variant with Sep incorporated at position 2 and AcK incorporated at position 101 (lower). Full spectra were listed as Figure S1 and S2.


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Figure 4

Figure 4. Expression of Sep/AcK-containing MDH. A) The Coomassie blue-stained SDSPAGE gel of purified full-length MDH and its variant. The same volumes of elution fractions were loaded on the SDS-PAGE gel. B) Western blotting of purified full-length MDH and its variants. The codons used for positions 140 and 280 were listed. The same amount of proteins were loaded on the gels. C) MALDI-TOF mass spectra of purified full-length MDH (upper) and its variant with AcK incorporated at position 140 and Sep incorporated at position 280 (lower). Full spectra were listed as Figure S5 and S6.



Figure 5

Figure 5. The structure and function of MDH. A) The relative enzyme activities of MDH and its variants. Mean values and standard deviations were calculated from three replicates. The enzyme activity of wild-type MDH was set as 1. B) The crystal structure of E. coli MDH tetramer (PDB ID: 3HHP). C) A zoom-in view of the dimer-dimer interface. D) A map of amino acids involved in the dimer-dimer interface.


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For Table of Contents Use Only

Genetically Incorporating Two Distinct Post-translational Modifications into One Protein Simultaneously Sumana Venkat, Jourdan Sturges, Alleigh Stahman, Caroline Gregory, Qinglei Gan, Chenguang Fan


Genetically Incorporating Two Distinct Post-translational Modifications

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