Article pubs.acs.org/ac
Microchip Zone Electrophoresis for High-Throughput Analysis of Monoclonal Antibody Charge Variants Tobias D. Wheeler,*,†,§ Jing Lucy Sun,†,§ Sina Pleiner,‡,§ Holger Geier,‡ Philine Dobberthien,‡ Joey Studts,‡ Rajendra Singh,† and Bahram Fathollahi*,† †
Microfluidics R&D, Caliper, a PerkinElmer Company, 850 Marina Village Parkway, Alameda California 94501, United States Department of Process Science, Boehringer Ingelheim Pharma GmbH & Co. KG, Birkendorfer Strasse 65, 88397 Biberach, Germany
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S Supporting Information *
ABSTRACT: A high-throughput screening assay on a microfluidic chip was developed for the determination of charge variants of monocolonal antibodies (mAbs) in pI range of 7−10. This method utilizes microchip zone electrophoresis for rapid separation (
