Microfluidic Print-to-Synthesis Platform for Efficient Preparation and

Apr 10, 2018 - The MPS printing platform is composed of a printer head, an X–Y traveling stage (DDS 220, Thorlabs Inc.), controlling circuits, and s...
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Microfluidic Print-to-Synthesis Platform for Efficient Preparation and Screening of Combinatorial Peptide Microarrays Jiannan Li, Randy P. Carney, Ruiwu Liu, Jinzhen Fan, Siwei Zhao, Yan Chen, Kit S. Lam, and Tingrui Pan Anal. Chem., Just Accepted Manuscript • DOI: 10.1021/acs.analchem.8b00371 • Publication Date (Web): 10 Apr 2018 Downloaded from http://pubs.acs.org on April 10, 2018

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Analytical Chemistry

Microfluidic PrintPrint-toto-Synthesis Platform for Efficient Preparation and Screening of Combinatorial Peptide Microarrays Jiannan Li1, Randy P. Carney2, Ruiwu Liu2, Jinzhen Fan1, Siwei Zhao1†, Yan Chen3, Kit S. Lam2*, and Tingrui Pan1* 1

Department of Biomedical Engineering, University of California, Davis, California, USA Department of Biochemistry and Molecular Medicine, University of California, Davis, California, USA 3 Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen, People's Republic of China 2

ABSTRACT: In this paper, we introduce a novel microfluidic combinatorial synthesis platform, referred to as Microfluidic Printto-Synthesis (MPS), for custom high-throughput and automated synthesis of a large number of unique peptides in a microarray format. The MPS method utilizes standard Fmoc chemistry to link amino acids on a polyethylene glycol (PEG)-functionalized microdisc array. The resulting peptide microarrays permit rapid screening for interactions with molecular targets or live cells, with low non-specific binding. Such combinatorial peptide microarrays can be reliably prepared at a spot size of 200 µm with 1 mm center-to-center distance, dimensions that require only minimal reagent consumption (less than 30 nL per spot per coupling reaction). The MPS platform has a scalable design for extended multiplexibility, allowing for 12 different building blocks and coupling reagents to be dispensed in one microfluidic cartridge in the current format, and could be further scaled up. As proof of concept for the MPS platform, we designed and constructed a focused tetrapeptide library featuring 2560 synthetic peptide sequences, capped at the N-terminus with 4-[(N’-2-methylphenyl)ureido]phenylacetic acid. We then used live human T lymphocyte Jurkat cells as a probe to screen the peptide microarrays for their interaction with α4β1 integrin overexpressed and activated on these cells. Unlike the one-bead-one-compound approach that requires subsequent decoding of positive beads, each spot in the MPS array is spatially addressable. Therefore, this platform is an ideal tool for rapid optimization of lead compounds found in nature or discovered from diverse combinatorial libraries, using either biochemical or cell-based assays.