Biochemistry 1992,31, 11835-1 1842
11835
Secondary Structure and Interactions of the Packaged dsDNA Genome of Bacteriophage P22 Investigated by Raman Difference Spectroscopy+ K. L. Aubrey,* S. R. Casjens,* and G. J. Thomas, Jr.*J Division of Cell Biology and Biophysics, School of Biological Sciences, University of Missouri-Kansas City, Kansas City, Missouri 641 10-2499, and Department of Cellular, Viral and Molecular Biology, University of Utah Medical Center, Salt Lake City, Utah 84132 Received May 15, 1992; Revised Manuscript Received August 25, 1992
ABSTRACT: Vibrational spectra of the double-stranded DNA genome of bacteriophage P22 in packaged and
unpackaged states are compared by digital difference Raman spectroscopy. The difference Raman spectrum, which is sensitive to structural changes at the level of
