ARTICLE
Spectral Analysis of Multiplex Raman Probe Signatures Barry R. Lutz,† Claire E. Dentinger,† Lienchi N. Nguyen,† Lei Sun,† Jingwu Zhang,† April N. Allen,‡ Selena Chan,†,* and Beatrice S. Knudsen‡,* †
Biomedical/Life Sciences, Digital Health Group, Intel Corporation, SC3-41 2200 Mission College Boulevard, Santa Clara, California 95054, and ‡Division of Public Health Sciences, Fred Hutchinson Cancer Research Center, M5-A864, 1212 Aloha Street, Seattle, Washington 98109
ABSTRACT Raman nanoparticle probes are an emerging new class of optical labels for interrogation of physiological and pathological processes in bioassays, cells, and tissues. Although their unique emission signatures are ideal for multiplexing, the full potential of these probes has not been realized because conventional analysis methods are inadequate. We report a novel spectral fitting method that exploits the entire spectral signature to quantitatively extract individual probe signals from multiplex spectra. We evaluate the method in a series of multiplex assays using unconjugated and antibody-conjugated composite organicⴚinorganic nanoparticles (COINs). Results show sensitive multiplex detection of small signals (
