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The Protein and Energy Metabolic Response of Skeletal Muscle to the Low-Protein Diets in Growing Pigs Yinghui Li, Fengna Li, Yehui Duan, Qiuping Guo, Wenlong Wang, Chaoyue Wen, Xingguo Huang, and Yulong Yin J. Agric. Food Chem., Just Accepted Manuscript • DOI: 10.1021/acs.jafc.7b02461 • Publication Date (Web): 15 Sep 2017 Downloaded from http://pubs.acs.org on September 18, 2017
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Journal of Agricultural and Food Chemistry is published by the American Chemical Society. 1155 Sixteenth Street N.W., Washington, DC 20036 Published by American Chemical Society. Copyright © American Chemical Society. However, no copyright claim is made to original U.S. Government works, or works produced by employees of any Commonwealth realm Crown government in the course of their duties.
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Journal of Agricultural and Food Chemistry
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The Protein and Energy Metabolic Response of Skeletal Muscle to the Low-Protein
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Diets in Growing Pigs
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Yinghui Li,†,‡,§ Fengna Li,*,‡,§ Yehui Duan,‡,# Qiuping Guo,‡,# Wenlong Wang,‡,⊗
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Chaoyue Wen,‡,⊗ Xingguo Huang,†,§ and Yulong Yin*,‡, ⊗
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†
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Hunan 410128, China
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‡
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Subtropical Agriculture, Chinese Academy of Sciences; Hunan Provincial Engineering
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Research Center for Healthy Livestock and Poultry Production; Scientific Observing and
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Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of
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Agriculture, Changsha, Hunan 410125, China
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§
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China
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#
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⊗Laboratory
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University, Changsha, Hunan 410018, China
College of Animal Science and Technology, Hunan Agricultural University, Changsha
Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of
Hunan Co-Innovation Center of Animal Production Safety, Changsha Hunan 410128,
University of Chinese Academy of Sciences, Beijing 100039, China of Animal Nutrition and Human Health, School of Biology, Hunan Normal
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Journal of Agricultural and Food Chemistry
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ABSTRACT: This study was conducted to determine the effect of the low-protein diets
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on protein and energy metabolism in skeletal muscle, and to elucidate the underlying
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mechanism. A total of 18 growing pigs (average initial body weight = 36.47 kg) were
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individually penned and assigned to three treatments, each treatment was fed one of three
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diets containing either 18%, 15%, or 12% CP. The results showed that reducing dietary
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CP contents decreased (P < 0.05) the weight of half Longissimus dorsi (LD) muscle and
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serum concentration of insulin-like growth factor 1 (IGF-1). Compared with the 18% and
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15% CP treatments, the 12% CP treatment suppressed (P < 0.05) the components of
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mammalian target of rapamycin complex 1 (mTORC1) pathway, but up-regulated (P
