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Environmentally relevant inocula concentrations improve the reliability of persistent assessments in biodegradation screening tests Timothy James Martin, Jason R. Snape, Abigail Bartram, Aidan Robson, Kishor Acharya, and Russell J. Davenport Environ. Sci. Technol., Just Accepted Manuscript • DOI: 10.1021/acs.est.6b05717 • Publication Date (Web): 26 Jan 2017 Downloaded from http://pubs.acs.org on February 3, 2017
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Environmental Science & Technology
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Environmentally relevant inocula concentrations
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improve the reliability of persistent assessments in
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biodegradation screening tests
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Timothy J Martin†*, Jason R. Snape‡, Abigail Bartram‡†, Aidan Robson†, Kishor Acharya†,
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Russell J. Davenport†
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† School of Civil Engineering and Geosciences, Cassie Building, Newcastle University,
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Newcastle upon Tyne, NE1 7RU, United Kingdom
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‡ AstraZeneca Global Environment, Mereside, Alderley Park, Macclesfield, Cheshire, SK10
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4TF, United Kingdom
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[email protected]; Telephone: +44 (0) 191 208 7091; Fax: +44 (0) 191 208 6502
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ABSTRACT
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Standard OECD biodegradation screening tests (BSTs) have not evolved at the same rate as
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regulatory concerns, which now place an increased emphasis on environmental persistence.
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Consequently, many chemicals are falsely assigned as being potentially persistent based on
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results from BSTs. The present study increased inoculum concentrations and test duration to
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more environmentally relevant levels, to assess their impact on biodegradation outcome and
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intra-test replicate variability for chemicals with known environmental persistency.
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Chemicals were assigned to potential persistence categories based on existing degradation
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data. These more environmentally relevant BSTs (erBSTs) improved the reliability of
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persistency assignment by reducing the high variability associated with these tests and the
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Environmental Science & Technology
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occurrence of failures at low inoculum concentrations due to the exclusion of specific
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degraders. Environmental fate was determined using a reference set of
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compounds with a range of potential environmental persistence and full mass balance data
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were collated. The erBST correctly assigned five reference chemicals of known
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biodegradabilities to their appropriate persistence category in contrast to a standard OECD
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Ready Biodegradation Test (RBTs, P
