Figure 3 (A,B) Gliotoxin dose-dependently inhibits linear polyubiquitylation of NEMO in TNF-α-stimulated Jurkat (A) and CD40L-stimulated Bal17.2 (B). The asterisks show heavy chain of anti-NEMO antibody. (C,D) Gliotoxin inhibits phosphorylation and degradation of IκBα in TNF-α-stimulated Jurkat (C) and CD40Lstimulated Bal17.2 (D). (E,F) Gliotoxin inhibits nuclear translocation of RelA in TNF-α-stimulated Jurkat (E) and CD40L-stimulated Bal17.2 (F). The asterisk shows a non-specific band. (G) Gliotoxin induces apoptosis of Jurkat in the presence of TNF-α. 140x145mm (300 x 300 DPI)
Figure 4 (A,B) Recruitment of LUBAC to TNF receptor signaling complex (TNFRSC) is suppressed by SM-164 but not by gliotoxin. (C) Gliotoxin suppresses TNF-α-stimulated IKKβ activation, which is thought to be induced by linear polyubiquitylation of NEMO. 140x94mm (300 x 300 DPI)
Gliotoxin suppresses NF-κB activation by selectively inhibiting linear ubiquitin chain assembly complex (LUBAC). 72x39mm (300 x 300 DPI)
