Anal. Chem. 1999, 71, 5348-5353
High-Resolution Capillary Isoelectric Focusing of Complex Protein Mixtures from Lysates of Microorganisms Yufeng Shen, Fan Xiang, Timothy D. Veenstra, Eliza N. Fung, and Richard D. Smith*
Environmental Molecular Sciences Laboratory, Pacific Northwest National Laboratory P.O. Box 999, Richland, Washington 99352
High-resolution capillary isoelectric focusing separations of complex protein mixtures have been obtained for cellular lysates of Saccharomyces cerevisiae, Eschericia coli, and Deinococcus radiodurans. High quality separations are shown to be achievable for total protein concentrations of
