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have been built and their Fourier transforms are being computed for comparison with the observed diffraction data. We wish to thank Prof. S. Ochoa for...
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COR.IMUNICATIONS TO THE

EDITOR

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0.4%; mol. wt., 139. Acetylation with acetic anhydride in acetic acid or ketene gave two isomeric N-acetyl derivatives : acetylhypoglycin, m.p. 92.5-95.5' (uncor.), [ a l 2 S ~ 28.5 (c 1.44, acetone); Calcd.for C9H13N03: C, 59.00; HI 7.15; N, 7.65; 1 C-CH3, 8.20; mol. wt., 183. Found: C, 58.95; H, 7.42; N, 7.93; neut. equiv., 186; Isoacetylhypoglycin, m.p. 120-121" (uncor.), [ c Y ] ~ ~ D - 0.08 f 0.06 (c 5.3, acetone). Found: C, 58.93; H, 7.19; N, 7.53; C-CH3, 6.59; mol. wt., 180; neut. equiv., 193. Lack of any maxima in the ultraviolet indicated the absence of conjugated double bonds. The infrared spectra of hypoglycin, its salts and acetyl derivatives were consistent with an amino acid formulation and displayed bands a t 11.25 and 5.70 p (weak) indicative of a terminal methylene group. Confirmatory evidence for the latter was found in the formation of formaldehyde (10% yield) on ozonolysis of isoacetylhypoglycin and in the formation of a C-methyl on reduction of the double bond (vide

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Fig. 1.-The ultraviolet spectra of equal concentrations polyinosinic acid, polycytidylic acid, and a 1 : l mixture of these solutions. All solutions are in 0.1 M iYaC1, 0.01 M sodium cacodylate, @I-I 6.7, T = 23".

which the bases are joined by systematic hydrogen bonding. Models of these helical configurations have been built and their Fourier transforms are being computed for comparison with the observed diffraction data. We wish to thank Prof. S. Ochoa for the gift of some polynucleotide phosphorylase which was used to prepare the polymers. We are indebted to Mrs. Jean Johnson for technical assistance. SECTION o s PHYSICAL CHEMISTRY DAVIDR. DAVIES h TISSTITUTE ~ OF MENTAL ~ HEALTH ~ ~ ~ BETHESDA, MARYLAND ALEXANDER RICH RECEIVED DECEMBER 6, 1957

THE STRUCTURE AND BIOLOGICAL ACTIVITIES OF HYF'OGLYCIN

Sir: Hypoglycin A, a substance with hypoglycemic activity, has been isolated from the seeds of Blighia sapida.'V2 It has been described as an amino acid with the empirical formula CTHllN02. Work in these laboratories confirms these reported findings and supports structure I for h y p ~ g l y c i n . ~ b, /CH2

CH2=C