IL-6 Antibody and RGD Peptide Conjugated Poly(amidoamine

Jun 16, 2016 - percentage of early apoptotic cells after free DOX, G4.5-RGD/. DOX, and G4.5-IL6/DOX treatment was 26.7, 62.5, and 29.7%, respectively ...
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Addition/Correction pubs.acs.org/JPCB

Correction to “IL‑6 Antibody and RGD Peptide Conjugated Poly(amidoamine) Dendrimer for Targeted Drug Delivery of HeLa Cells” Shewaye Lakew Mekuria, Tilahun Ayane Debele, Hsiao-Ying Chou, and Hsieh-Chih Tsai* J. Phys. Chem. B 2016, 120 (1), 123−130. DOI: 10.1021/acs.jpcb.5b11125 In the Experimental Section, the following should be corrected as follows:

Also, the caption description in Figure 6 is wrongly designated as “The lower-left and upper-left quadrants in each section indicate the populations of normal cells and early necrotic cells, respectively, whereas the lower-right and upper-right quadrants in each panel indicate the populations of early and late apoptotic cells, respectively.” Instead, it should be corrected as “The lower-left and upper-left quadrants in each section indicate the populations of normal cells and early apoptotic cells, respectively, whereas the lower-right and upper-right quadrants in each panel indicate the populations of necrotic and late apoptotic cells, respectively.” The qualitative data is correctly labeled. In addition, in the Results and Discussion section, “The percentage of early apoptotic cells after free DOX, G4.5-RGD/ DOX, and G4.5-IL6/DOX treatment was 26.7, 62.5, and 29.7%, respectively,” should be changed to “The percentage of necrosis after free DOX, G4.5-RGD/DOX, and G4.5-IL6/DOX treatment was 26.7, 62.5, and 29.7%, respectively.”

encapsulation efficiency (%) initial fluorescence intensity = × 100 initial fluorescence intensity − final fluorescence intensity

should be corrected by encapsulation efficiency (%) initial fluorescence intensity − final fluorescence intensity = × 100 initial fluorescence intensity

and cell viability (%) =

absorbance of control cells × 100 absorbance of treated cells

should be corrected by cell viability (%) =

absorbance of treated cells × 100 absorbance of control cells

Figure 6. Apoptotic/necrotic cell populations as determined by flow cytometric analysis with Annexin V-Alexa fluor 488 and propidium iodide (PI) staining after incubating HeLa cells with (A) PBS as control, (B) free DOX, (C) G4.5-RGD/DOX, and (D) G4.5-IL6/DOX. The lower-left and upperleft quadrants in each section indicate the populations of normal cells and early apoptotic cells, respectively, whereas the lower-right and upper-right quadrants in each panel indicate the populations of necrotic and late apoptotic cells, respectively. Published: June 16, 2016 © 2016 American Chemical Society

5786

DOI: 10.1021/acs.jpcb.6b05310 J. Phys. Chem. B 2016, 120, 5786−5786