Interactions of Proteins with Disulfide Compounds: Some Implications

May 1, 2002 - Irving M. Klotz, Janet Ayers, J. Y. C. Ho, Myer G. Horowitz, Richard E. Heiney. J. Am. Chem. ... M. J. TAIT , F. FRANKS. Nature 1971 230...
2 downloads 0 Views 1MB Size
21 32

1. AI, KLOTZ,J. AYERS,J. Y.C. Ho, IZI. G. HOROWITZ AND R . E. HEINEV [CONTRIBUTION FROM

THE

Vol. SO

CHEMICAL LABORATORY OF NORTHWESTERN UNIVERSITY]

Interactions of Proteins with Disulfide Compounds : Some Implications for Electron Transport in Proteins BY IRVIKG M. KLOTZ,JANET XYERS, J. Y. C. Ho, MYERG. HOROWITZ A N D RICHARD E. HEINEY RECEIVED NOVEMBER 29, 1957 The reactions of serum albumin, 0-lactoglobulin and ovalbumin with a disulfide compound have been studied by photometric titrations and by equilibrium dialysis methods. The thiols of ovalbumin react with the disulfide in a manner analogous to the reaction of small-molecule mercaptans. On the other hand, the -SH groups of serum albumin or @-lactoglobulin react Kith the disulfide t o give mixed disulfides exclusively in a manner that has a stoichiometry and other features that are difficult t o explain by any simple mechanism. It is suggested that the electrons which are transferred in this reaction (as well as in other oxidation-reduction reactions) are transported by a shift of an H- through the hydration lattice of the protein molecule. The possible significance of such a mechanism in energy transport in biochemical systems is discussed.

Introduction Past investigations of soluble protein coniplexes with small molecules have dealt largely with reversibly-bound substances. It has seemed likely that some further insight into protein structure and behavior might be obtained from a study of complexes formed through covalent bonds. A promising reaction in this connection is that between protein mercaptan groups and disulfides, since the reaction occurs readily a t room temperature and since there exists a substantial background of quantitative information on the corresponding reaction in simpler systems. This mercaptan interchange in proteins P-SH + R-S-S-R+P-S-S-R 4- R-SH (1) is specifically of interest as a possible mechanism for a variety of protein aggregation reactions. It was suggested originally by Huggins, Tapley and Jensenl as an explanation of the gelation of plasma albumin in urea. It has been proposed subsequently to account for cross-linking exchange phenomena in keratin, ~ 0 0 1ovalbumin* ,~ and fibrin.5 Furthermore, from another viewpoint this interchange is essentially an oxidation-reduction reaction and hence its course in a protein environment may provide some clues as to the mechanism oi protein participation in other oxidation-reduction reactions. It seems worthwhile, therefore, to study this interchange reaction directly. I n non-protein systems, mercaptan-disulfide interchange in small molecules has been investigated extensively, quantitative studies of the equilibria having been reported by Bersin and Steudel6 and more recently by Kolthoff, Stricks and Kapoor,? by Fava, Iliceto and Camera,* and by Eldjarn and Pihl.g The results from these simple systems cannot be carried over directly to proteins, however, as is immediately evident from the fact that many proteins with available mercaptan groups give no evidence of intermolecular disulfide interchange under normal conditions, even (1) C. Huggins, D. F. Tapley a n d E. V. Jensen, N a t u r e , 167, 592 (1951). (2) A. M. Woodin, Biochem. J . , 67, 99 (1954). (3) R. W. Burley, Natirre, 176, 510 (1955). (4) hl. Halwer, THISJ O U R N A L , 76, 183 (1954). ( 5 ) A. Loewy nnd J. T. Edsall, J. Bioi. Cham., 211, 829 (1954). ( 6 ) T. Rersin and J. Steudel, Ber.. 17B, 1015 (1938). (7) I . h l . Rulthoff, W. Stricks and R . C. Kapoor, T i m JOIJRNAI., 77, 4733 (1955). ( 8 ) A. F a v a , A. Iliceto and E. Camero, i b i d . , 79, 833 (1957). ( 0 ) I