Iodinated Analogs of Trimetoquinol as Highly Potent and Selective β2

of the 4'-substituent of 3',5'-diiodo analogs increased β2-AR affinity with little or no ... Sandeep S. Vansal, Gamal Shams, Dennis R. Feller, an...
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J. Med. Chem. 1996, 39, 3701-3711

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Iodinated Analogs of Trimetoquinol as Highly Potent and Selective β2-Adrenoceptor Ligands Joseph E. De Los Angeles,† Victor I. Nikulin,†,‡ Gamal Shams,§ Anish A. Konkar,| Ratna Mehta,| Dennis R. Feller,§ and Duane D. Miller*,† Department of Pharmaceutical Sciences, College of Pharmacy, University of Tennessee-Memphis, Memphis, Tennessee 38163, Divisions of Medicinal Chemistry/Pharmacognosy and Pharmacology, College of Pharmacy, The Ohio State University, Columbus, Ohio 43210, and Department of Pharmacology, Research Institute of Pharmaceutical Sciences School of Pharmacy, University of Mississippi, University, Mississippi 38677 Received March 14, 1996X

A series of trimetoquinol (1, TMQ) analogs were designed and synthesized based on the lead compound 2, a diiodinated analog of trimetoquinol which exhibits improved selectivity for β2versus β1-adrenoceptors (AR). To determine the influence of 1-benzyl substituents of trimetoquinol on β2-AR binding affinity and selectivity, we replaced and/or removed the 3′-, 4′-, and 5′-methoxy substituents of trimetoquinol. Replacement of the 4′-methoxy group of 2 with an amino (21c) or acetamido (15) moiety did not significantly alter β2-AR and thromboxane A2/ prostaglandin H2 (TP) receptor affinity. Substitution with a 4′-hydroxy (18) or -iodo (21b) group did not significantly alter β2-AR affinity, but greatly reduced TP receptor affinity (380- and 1200-fold, respectively). Further, the β2-AR can accommodate larger substituents such as a benzamide at the 4′-position (26b). Other monoiodo derivatives (24, 26a) have similar or slightly lower affinity to both β2-AR and TP receptor compared to their diiodo analogs. Interestingly, removal of the 4′-substituent of 3′,5′-diiodo analogs increased β2-AR affinity with little or no effect on β1-AR and TP binding. Thus, analog 21a displayed highly potent (pKi 9.52) and selective (β2/β1 ) 600) binding affinity for β2-AR. On the other hand, trifluoromethyl substituents at the 3′- and 5′-positions (27) essentially abolished binding affinity at β2-AR and TP receptors. The differential binding effects of the aforementioned trimetoquinol modifications on the receptor systems may reflect differences in the binding pocket that interacts with the benzyl portion of trimetoquinol analogs. Thus, manipulation of the 1-benzyl moiety of trimetoquinol (1) has resulted in analogs that exhibit potent β2-AR binding affinity and significantly lower β1-AR and TP receptor affinities. Introduction Trimetoquinol (1) is a potent nonspecific β-adrenoceptor (β-AR) agonist clinically used in Japan as a bronchorelaxant (Figure 1).1 Optical resolution of trimetoquinol and subsequent evaluation of the stereoisomers revealed that the (S)-(-)-isomer of trimetoquinol is a potent β-AR agonist in heart and lung tissues whereas the (R)-(+)-isomer acts as a selective and highly stereospecific TP receptor antagonist.2-5 Radioligand competition binding studies at β-AR and TP receptors show high stereoselective binding (>100-fold) for the (S)(-)-isomer and (R)-(+)-isomer, respectively. This stereoselectivity is also observed in the binding of fluorinated trimetoquinol analogs at β-AR.6 The basic structure of catecholamines, such as norepinephrine and the β-adrenoceptor agonist isoproterenol, is incorporated within the tetrahydroisoquinoline nucleus of trimetoquinol. In studies using mutated hamster β2-AR expressed in Chinese hamster ovary (CHO) cells, replacement of Asp113 with Asn113 abolished receptor binding of trimetoquinol and its analogs.7 In addition, replacement of Ser204 and Ser207 with Ala204 and Ala207 decreased the binding affinity of trimetoquinol analogs in β2-AR to a lesser extent, but †

University of Tennessee-Memphis. Permanent address: Institute of Chemical Physics, Chernogolovka, Moscow Region 142432, Russia. § University of Mississippi. | The Ohio State University. X Abstract published in Advance ACS Abstracts, July 15, 1996. ‡

S0022-2623(96)00208-7 CCC: $12.00

Figure 1.

greatly diminished their ability to stimulate cAMP accumulation.7 However, both the binding and functional activities of isoproterenol are significantly reduced in the β2-AR Asn113, Ala204, and Ala207 mutants. These results suggest that, although trimetoquinol analogs may interact with the same amino acid residues in the binding site as isoproterenol, the contribution of catechol interactions with these mutated β2-ARs is less significant in terms of ligand binding and may well be overshadowed by the binding contributions of the trimethoxybenzyl group of trimetoquinol. In previous studies, substitution with fluorine or iodine on the 5- or 8-positions of trimetoquinol resulted in only a modest (∼10-fold) increase in β2-AR versus β1AR selectivity as compared to trimetoquinol in functional and binding studies.6,8 In addition, we have also found that replacement of the 3′- and 5′-methoxy substituent of trimetoquinol with iodine atoms (i.e., 2) is © 1996 American Chemical Society

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Scheme 1a

a (a) Toluene, reflux (Dean-Stark trap), 72 h; (b) POCl , MeCN, reflux; (c) NaBH , MeOH; (d) TFAA, THF (6a) or (Boc) O, NaOH, THF 3 4 2 (6b); (e) H2, Pd/C (7a) or Raney Ni (7b); (f) 1 equiv of BTMACl2I, MeOH, CH2Cl2, 20 h (8a,b), or 4 equiv of BTMACl2I, CaCO3, MeOH, CH2Cl2, 3 days (8a); (g) 1. BBr3, CH2Cl2, 2. MeOH.

well tolerated on both β-AR8 and TP receptors.9,10 Interestingly, although its binding affinity at β1-AR is slightly better than trimetoquinol, analog 2 displays a much higher affinity than trimetoquinol for β2-AR. These earlier findings suggest that trimetoquinol analogs interact with an auxiliary site through the substituted benzyl group in addition to the binding site shared by catecholamines. This subsite can be taken advantage of in the development of more site-selective agents. The high potency of 2 seems to suggest that this auxiliary site is hydrophobic in nature. On TP receptors, the complementary binding sites for trimetoquinol analogs are essentially unknown. However, compound 2 is a more potent TP receptor antagonist than trimetoquinol, further suggesting that 1-benzyl ring modifications are appropriate to develop agents with greater selectivity on β-AR versus TP receptors and vice versa. In this report, we describe the synthesis and evaluation of iodinated trimetoquinol analogs designed as probes for characterizing the receptor binding interactions associated with the benzyl substituent of trimetoquinol analogs and as site-selective β-AR and TP ligands. These chemical modifications are expected to provide us with a greater separation of the pharmacological activities for this class of compounds. Siteselective β-AR agents have potential in the treatment of cardiopulmonary diseases, non-insulin-dependent diabetes (type II), and obesity;11 whereas highly selective TP receptor antagonists have value in the treatment of thrombolytic disorders.5,9,12 Chemistry We have composed a convenient protection scheme for the synthesis of the desired trimetoquinol analogs.

The triple-protected isoquinoline intermediates were synthesized as shown in Scheme 1. The tetrahydroisoquinolines 6a-c were synthesized from the O-methylor O-benzyl-protected catecholamines 3a or 3b, respectively, and 4-nitrophenylacetic acid (4a) or 3,5-bis(trifluoromethyl)phenylacetic acid (4b) using methods described previously.6,10,13 The amino groups of 6a and 6b were protected with trifluoroacetyl (TFA) and tertbutyloxycarbonyl (t-BOC), respectively. The nitro groups of 7a,b were reduced via catalytic hydrogenation using Pd/C or Raney Nickel, respectively, to give the aniline derivatives 8a,b. Iodination of 8a,b with 1 equiv of benzyltrimethylammonium dichloroiodate (BTMACl2I) according to Kajigaeshi et al.14 led to the 3′-iodo analogs 9a,b. An additional 3 equiv of BTMACl2I added in portions over a 3 day period was required to convert 8a completely to the diiodo derivative 10a. Interestingly, the diiodo product 10a was often isolated as light pink to reddish crystals. We found that the minor side product 11 (Scheme 2) was responsible for the reddish coloration. TLC analysis of the reaction mixture and isolated crude product indicates that compound 11 is formed mostly during workup. Compound 11 was isolated by flash chromatography. The structure of 11 and its deacetylation product 12 was proven by 1H and 13C NMR and elemental analysis. Compound 11 was also isolated in an attempt to convert the 4′-amino of 10a to a hydrazine group. Thus, diazotization of 10a followed by reduction with H2SO3 gave compound 11 as the only isolated product in low yield. While reaction of 10a with acetic anhydride at room temperature did not give the desired 4′-acetamido derivative 13, heating 10a in acetic anhydride at reflux resulted in the diacetylation product 16 (Scheme 3).

Iodinated Analogs of Trimetoquinol

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Scheme 2a

a

(a) 5 equiv of BTMACl2I, MeOH, CH2Cl2, 5 days; (b) K2CO3, MeOH, H2O; (c) 1. NaNO2, H2SO4, AcOH, 2. H2SO3.

Scheme 3a

a (a) AcCl, Et N, DMAP; (b) K CO , MeOH, H O; (c) 1. BBr , CH Cl , 2. MeOH; (d) Ac O, reflux; (e) 1. NaNO , H SO , AcOH, 2. 3 2 3 2 3 2 2 2 2 2 4 H3PO2 or KI.

Similar diacetylation has been reported with the reaction of 2,6-dibromo-4-toluidine with refluxing acetic anhydride while lower temperatures gave a mixture of mono- and diacetylated products.15 With this result in mind, monoacetylation was accomplished by reacting 10a with 5 equiv of acetyl chloride in the presence of 4-(dimethylamino)pyridine (DMAP) and triethylamine at room temperature to afford 13. Basic hydrolysis of the trifluoroacetyl protecting group of 10a and 13 gave 20c and 14, respectively. The methoxy derivatives 20c,

14, and 6c were demethylated with BBr3 to afford the desired trimetoquinol analogs 21c, 15, and 27, respectively, as hydrobromide salts (Schemes 1 and 3). In a similar manner, the 6,7-bis(benzyloxy)-1-(3,5-diiodo-4methoxybenzyl)-1,2,3,4-tetrahydroisoquinoline10 (17) was dealkylated with BBr3 to give 6,7-dihydroxy-1-(3,5-diiodo-4-hydroxybenzyl)-1,2,3,4-tetrahydroisoquinoline (18), the demethyl analog of 2. Diazotization of 10a (Scheme 3) followed by reaction of the diazonium salt with H3PO2 or potassium iodide (KI) gave the diiodo and triiodo

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Scheme 4a

a

(a) AcCl, Et3N; (b) K2CO3, MeOH, H2O; (c) 1. BBr3, CH2Cl2, 2. MeOH; (d) Ac2O, 4 or PhCOCl, Et3N; (e) 1. TMSI, MeCN, 2. MeOH.

derivatives, 19a and 19b, respectively. Basic hydrolysis of the trifluoroacetyl group of 19a,b as before gave 20a,b. Demethylation of 20a,b with BBr3 proceeded smoothly to give 21a,b. Compound 9a was acylated with acetic anhydride in refluxing benzene to give 22 which was deprotected in the same manner as 14 to give 23 (Scheme 4). However, attempts to demethylate 23 with BBr3 failed to give the desired product 26a. Surprisingly, the amide bond of 23 was cleaved to give aniline 24. This indicates the importance of both o-iodine atoms as a sterical hindrance toward cleavage of the acetamido group of 14 by BBr3. Thus, we turned our attention to trimethylsilyl iodide (TMSI) as a mild reagent for ether cleavage. However, this agent was too weak to effect demethylation of 23; therefore, the catechol O-methyl ether protecting groups were changed to benzyl ethers. Hence, compounds 26a and 26b were prepared from the O-benzyl and N-t-BOC protected 9b (Scheme 4). The acylated compounds 25a and 25b were deblocked using TMSI. Initially, using the procedure of Lott16 (TMSI, MeCN, 50 °C 2 h), amide 25a gave the desired amide 26a along with a significant amount of the deacetylation product 24. Ordinarily, amides are stable to TMSI. To optimize the selectivity, we monitored the TMSI deprotection reaction by NMR spectroscopy at room temperature. The O-benzyl protecting groups were removed within 6 h, and no cleavage of the amide bond was observed at this temperature for 20 h. Thus, using the reaction conditions 4-6 equiv of TMSI, MeCN, room temperature, 6 h, we obtained 26a and 26b from 25a and 25b, respectively. The proton NMR spectra of synthesized compounds were quite complicated, especially the 2-t-BOC derivatives which displayed complex splitting patterns reflecting two relatively stable conformations with ratios ranging from 5:2 to 5:4, similar to those observed for N-Ac- and N-Me-substituted tetrahydroisoquinolines.17,18 However, the 13C NMR spectra of 1-benzyltetrahydroisoquinolines can be easily used for structure identification because of their relative simplicity. Assignments of signals (final compounds) were made based on the 13C NMR spectra of salsolinol,19 effects of substituents on the benzene ring, and off-resonance spectra. For 2-TFA derivatives, the chemical shift of the C-3 atom appears as a quartet (4JC-F ≈ 3.7 Hz) indicating its close proximity to the CF3 group.

Results and Discussion We have modified the trimethoxybenzyl portion of trimetoquinol by replacing one or more of the methoxy groups with a variety of halogenated substitutions. The effects of these modifications on the receptor binding affinity of trimetoquinol analogs (Table 1) for human β2-AR expressed in CHO cells and human TP receptors (platelets) were determined by radioligand competition binding assays using [125I]iodocyanopindolol (ICYP) and [3H]-SQ 29548 as radioligands, respectively. β2-Adrenoceptors. In this study, most of the modifications made on the trimethoxybenzyl portion of trimetoquinol resulted in enhancement of β2-AR affinity. Previously, it was shown that replacement of the 3′- and 5′-methoxy groups of trimetoquinol with iodines [i.e., 1 (pKi ) 7.36) f 2 (pKi ) 8.69)] resulted in a greater than 20-fold increase in affinity.8 In the present study, complete replacement of the 3′-, 4′-, and 5′-methoxy groups of trimetoquinol (1) with iodine atoms to give the triiodo analog 21b (pKi ) 8.82) enhanced β2adrenoceptor affinity 29-fold versus trimetoquinol (1), but with respect to 2, the additional iodine substituent at the 4′-position adds little to the binding affinity. Studies on human β2-AR indicate that 4′-position substituents reflecting varying size and chemical properties are well tolerated. Replacement of the 4′-methoxy of 2 with an amino group [i.e., 2 f 21c (pKi ) 8.81)] did not significantly alter affinity, and replacement with a 4′-acetamido [i.e., 15 (pKi ) 8.06)] reduced affinity only 4-fold. A similar replacement with a hydroxy (i.e., 18, pKi ) 7.93) reduced affinity about 5-fold as compared to 2. The receptor binding pocket that interacts with substituents at the 4′-position seems to be sufficiently large to accommodate the 4′-benzamido moiety of 26b (pKi ) 8.70). Interestingly, the diiodo analog 21a (pKi ) 9.52), which lacks a 4′-substituent, exhibits the most potent affinity with a Ki value in the subnanomolar range. Thus, while a wide range of substituents at the 4′-position are accepted by the receptor binding pocket, these 4′-substituents contribute little to binding affinity. Based on the present binding data, this binding pocket is best left unoccupied for maximum binding affinity; on the other hand, we are carrying out further investigations to find the optimum substituent at the 4′position that will take advantage of the pocket in this region for additional binding interactions.

Iodinated Analogs of Trimetoquinol

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Table 1. Human β2-Adrenoceptors (AR) Expressed in CHO Cells and Platelet Thromboxane A2/Prostaglandin (TP) Receptor Binding Affinities of Trimetoquinol (TMQ) Analogs

human β2-AR CHOa

1-benzyl substituents

human TP receptorsb

compound

R1

R2

R3

pKi ( SEM

PRc

1 2 21c 24 15 26a 21a 21b 26b 18 27

OCH3 I I I I I I I I I CF3

OCH3 OCH3 NH2 NH2 NHCOCH3 NHCOCH3 H I NHCOPh OH H

OCH3 I I H I H I I H I CF3

7.36 ( 0.23 8.69 ( 0.16 8.81 ( 0.15 8.19 ( 0.27 8.06 ( 0.13 8.11 ( 0.16 9.52 ( 0.13 8.82 ( 0.18 8.70 ( 0.03 7.93 ( 0.03 5.36 ( 0.32

1.0 21 28 6.8 5.0 5.6 150 29 22 3.7 0.01

pKi ( SEM

PRc

6.79 ( 0.09 7.33 ( 0.07 6.73 ( 0.12 6.00 ( 0.02 6.45 ( 0.11 5.83 ( 0.14 6.75 ( 0.07 4.22 ( 0.03 5.27 ( 0.13 4.72 ( 0.09 4.08 ( 0.02

1.00 3.5 0.87 0.16 0.46 0.11 0.91 0.003 0.03 0.009 0.002

a Using [125I]ICYP as radioligand, N ) 4-9. b Using [3H]-SQ 29548 as radioligand, N ) 4-9. c PR ) potency ratio relative to 1 (TMQ). PR ) antilog [pKi(drug) - pKi(TMQ)].

Apparently, one m-iodo substituent is sufficient to retain high affinity since removing one of the iodo groups of either 21c or 15 [i.e., 21c f 24 (pKi ) 8.19) or 15 f 26a (pKi ) 8.11)] resulted in only minor shifts in affinity. To determine the nature (hydrophobic or electronic) of the binding contributions of 3′- and 5′substituents (methoxy and iodo), we synthesized the bis(trifluoromethyl) analog 27. While the hydrophobic property (π) of the trifluoromethyl group (π ) 0.88) is similar to iodine (π ) 1.12), this functional group exerts a much stronger electron-withdrawing effect. The binding affinity of the bis(trifluoromethyl)analog 27 (pKi ) 5.36) was 4 orders of magnitude lower than the diiodo analog 21a. Thus, trifluoromethyl substituents at the 3′- and 5′-positions abolish binding affinity. Since a trifluoromethyl group is similar in size to an iodine atom, the significantly stronger electron-withdrawing property of the trifluoromethyl (σp ) 0.54 versus σp ) 0.18 for iodine) is likely responsible for the greatly reduced binding affinity of 27. The electron-withdrawing effect of the trifluoromethyl substituents on the π-electron system of the aromatic ring may interfere with its capability to form aromatic interactions with the receptor binding site. These aromatic interactions may be more important for binding than hydrophobic interactions. β2/β1 Selectivity. Although replacement of the 3′and 5′-methoxy groups of trimetoquinol 1 with iodine atoms (i.e., 2) resulted in a 21-fold increase in β2-AR affinity, a similar increase in binding affinity was not observed for β1-AR (Table 2). As a result, the diiodo analog 2 exhibits moderate (ca. 40-fold) selectivity for β2-AR versus β1-AR. More importantly, the influence of a 4′-substituent is markedly different for β2-AR versus β1-AR. While the absence of a 4′-substituent (i.e., 21a) does not significantly alter β1-AR affinity (pKi ) 6.74), the same feature increased β2-AR affinity. Consequently, analog 21a displays more than 600-fold selectivity for β2-AR versus β1-AR, and is the most selective trimetoquinol analog yet reported. These results indicate a remarkable difference in the receptor binding site

Table 2. Selectivity of Trimetoquinol (TMQ) Analogs for Human β2- and β1-Adrenoceptors (AR) Expressed in CHO Cells pKi ( SEM compound human β1 CHOa human β2 CHOa β2/β1 selectivityb 1 2 21a

6.49 ( 0.06 7.10 ( 0.06 6.74 ( 0.30

7.36 ( 0.23 8.69 ( 0.16 9.52 ( 0.13

7.4 39 600

a Using [125I]ICYP as radioligand for β - and β -AR expressed 1 2 in CHO cells, N ) 4-9. b β2/β1 selectivity ) Ki(β1-AR)/Ki(β2-AR).

or pocket of β2- and β1-AR that interacts with substituents at the 4′-position of trimetoquinol analogs. TP Receptors. In general, replacement of the 3′and 5′-methoxy groups of trimetoquinol (1, pKi ) 6.79) with iodine to give analog 2 (pKi ) 7.33) resulted in only a slight increase (3-fold) in affinity. However, replacement of all three methoxy groups of trimetoquinol with iodines to give the triiodo analog 21b (pKi ) 4.22) practically abolished binding to TP receptors. In addition, demethylation of the 4′-methoxy substituent of 2 to give 18 (pKi ) 4.72) resulted in a similar 380-fold reduction in binding affinity. The very low binding affinity of 18 contrasts with a recent observation20 where 6,7-dihydroxy-1-(4′-hydroxy-3′-nitrobenzyl)-1,2,3,4tetrahydroisoquinoline exhibited good binding affinity. By contrast, substitution of the same methoxy group with an amino moiety (i.e., 21c, pKi ) 6.73) resulted in only a 3-fold decrease in affinity. Interestingly, removal of the 4′-substituent of 2 or 21c to give 21a (pKi ) 6.75) did not affect binding affinity significantly. Acetylation of the 4′-amino group of 21c was also tolerated as 15 (pKi ) 6.45) displayed binding affinity similar to 21c. Thus, while a primary amine, acetamide, or a methoxy group is tolerated at the 4′-position, a free hydroxy group or an iodo group is detrimental to binding affinity. Removal of one of the iodines of 21c and 15 to give 24 (pKi ) 6.00) and 26a (pKi ) 5.83), respectively, resulted in a 5-fold decrease in binding affinity, suggesting that hydrophobic interactions of 3′- or 5′-substituents contribute to binding. However, replacement of the 3′- and 5′-iodo groups of 21a with similarly hydrophobic tri-

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fluoromethyl substituents resulted in a drastic reduction in binding affinity. As with β2-AR, in terms of contribution to the overall binding affinity, hydrophobic interactions appear secondary to aromatic interactions.

residue was taken up in CH2Cl2. The solution was washed consecutively with 0.1 N HCl (30 mL), H2O (50 mL), 0.1 N NaOH (30 mL), and H2O (50 mL) and dried over MgSO4. The solvent was evaporated, and the crude solid was recrystallized from toluene to give 3.44 g (79%) of the product as white needles: mp 127-128 °C; 1H NMR (CDCl3) δ 7.79 (s, 1H, ArH), 7.72 (s, 2H, ArH), 6.75 (d, J ) 8.1 Hz, 1H, ArH), 6.67 (d, J ) 1.9 Hz, 1H, ArH), 6.61 (dd, J ) 8.1 & 1.9 Hz, 1H, ArH), 5.55 (m, 1H, NH), 3.85 (s, 3H, OMe), 3.84 (s, 3H, OMe), 3.58 (s, 2H, CH2), 3.52 (q, J ) 6.9 Hz, 2H, CH2N), 2.75 (t, J ) 6.9 Hz, CH2); 13C NMR (CDCl3) δ 168.72, 149.20, 147.87, 137.28, 131.90, 130.79, 129.47, 123.16, 121.20, 120.56, 111.72, 111.27, 55.83, 42.85, 40.90, 34.97; IR (KBr) 3323 (NH), 1651 (CdO) cm-1. Anal. (C20H13F6NO3) C, H, N. 6,7-Dimethoxy-1-(4-nitrobenzyl)-1,2,3,4-tetrahydroisoquinoline (6a). A mixture of 5a (8.0 g, 23.2 mmol) and POCl3 (15.6 mL, 167.4 mmol) in dry MeCN (160 mL) was heated at reflux for 4 h. The solvent was evaporated in vacuo to give a glassy residue which was taken up in methanol (250 mL) and evaporated to dryness 3 times until the residue was a solid. The solid residue was dissolved in MeOH (250 mL) and then cooled in an ice bath. Excess NaBH4 (17.56 g, 167.4 mmol) was carefully added in portions. The mixture was stirred at room temperature overnight. The solvent was removed in vacuo, and the solid residue was partitioned in CH2Cl2 (250 mL) and H2O (150 mL). The layers were separated, and the H2O layer was extracted with CH2Cl2 (100 mL). The combined organic fraction was washed successively with H2O (2 × 50 mL), 2 N NaOH (2 × 50 mL), and H2O (50 mL) and dried with Na2SO4. The solvent was evaporated to give a reddish oil. The oil was taken up in a minimum amount of methanol. The product crystallized upon standing and was collected by filtration (3.02 g, 40%): mp 134-136 °C; 1H NMR (CDCl3) δ 8.18 (d, J ) 8.7 Hz, 2H, ArH), 7.43 (d, J ) 8.7 Hz, 2H, ArH), 6.62 (s, 1H, ArH), 6.61 (s, 1H, ArH), 4.44 (dd, J ) 9.5, 4.1 Hz, ArCH-N), 3.87 (s, 3H, OMe), 3.84 (s, 3H, OMe), 3.28 (dd, J ) 13.7, 4.1 Hz, 1H, ArCH2), 3.23-3.15 (m, 1H, NCH), 3.04 (dd, J ) 13.7, 9.5 Hz, 1H, ArCH), 3.00-2.91 (m, 1H, NCH), 2.71 (m, 2H, ArCH2); IR (KBr) 3337 (NH), 1515, 1345 (NO2) cm-1. Anal. (C18H20N2O4) C, H, N. 6,7-Dimethoxy-1-[3,5-bis[(trifluoromethyl)benzyl]]1,2,3,4-tetrahydroisoquinoline Hydrochloride (6c‚HCl). The amide 5c (1.31 g, 3 mmol) was cyclized in the same manner as 6a (7 mL of 1 M HCl in ether was added to the methanolic solution of a crude product) to give 6c (0.84 g, 60%) as a hydrochloride salt: mp 104-115 °C (MeOH-ether); 1H NMR (CDCl3) δ 10.34 (bs, 2H, NH), 7.82 (s, 1H, ArH), 7.75 (s, 2H, ArH), 6.61 (s, 1H, ArH), 5.87 (s, 1H, ArH), 4.77 (m, 1H, CH), 3.91 (m, 1H, CH), 3.84 (s, 3H, OMe), 3.47 (s, 3H, OMe), 3.44 (m, 2H, CH), 3.28 (m, 2H, CH), 3.02 (m, 1H, CH); 13C NMR (CDCl3) δ 149.29, 147.74, 138.74, 132.08, 130.39, 123.33, 123.07, 121.40, 111.71, 109.43, 55.92, 55.38, 54.94, 40.46, 38.30, 24.80; IR (KBr) 3600-2400 (NH2), 1614, 1522 cm-1. Anal. (C20H19F6NO2‚HCl‚0.5H2O) C, H, N. 6,7-Dimethoxy-1-(4-nitrobenzyl)-2-(trifluoroacetyl)1,2,3,4-tetrahydroisoquinoline (7a). A solution of 6,7dimethoxy-1-(4-nitrobenzyl)-1,2,3,4-tetrahydrisoquinoline (6a) (3.0 g, 9.14 mmol) in dry THF (150 mL) was added to trifluoroacetic anhydride (20 mL) with stirring at 0 °C. The mixture was stirred at room temperature overnight with the flask equipped with a CaCl2 drying tube. The reaction mixture was poured onto ice (200 g) and the mixture stirred for 30 min. CH2Cl2 (200 mL) was added, and stirring was continued for 10 min. The layers were separated, and the organic layer was washed consecutively with H2O (50 mL), 0.2 N NaOH (100 mL), and H2O (100 mL) and then dried with Na2SO4. The solvent was evaporated in vacuo to give a yellow solid. Recrystallization from EtOAc-MeOH gave 1.94 g (50%) of yellow crystals: mp 162-164 °C; 1H NMR (CDCl3) δ 8.14 (m, 2H, ArH), 7.28 (m, 2H, ArH), 6.62 (s, 1H, ArH), 6.34 (s, 1H, ArH), 5.64 (t, J ) 6.7 Hz, ArCH-N), 3.87 (s, 3H, OMe), 3.72 (s, 3H, OMe), 3.3-3.6 (m, 2H, N-CH2), 3.25 (d, 2H, ArCH2), 2.98-2.6 (m, 2H, ArCH2); IR (KBr) 1686 (CdO), 1519, 1340 (NO2) cm-1; MS m/e (M+) 423 (M+H, FAB). Anal. (C20H19F3N2O5) C, H, N.

Conclusions In this study, we have shown that substitution on the trimethoxybenzyl portion of trimetoquinol (1) has a major role in the type and potency of biological activity expressed. 3′- and 5′-diiodo substitution on the 1-benzyl moiety significantly improved binding affinity at β2-AR, while having very little effect on β1-AR and TP receptor binding. The role of the 4′-position is particularly interesting in that the binding pocket that interacts with this substituent is more discriminating in TP receptors, while that of the β2-AR can accommodate more varied groups. Our studies indicate that these modifications of trimetoquinol (1) have provided a further separation of β2-AR versus TP receptor affinities, and the presence of 3′,4′,5′-triiodo substitution on the 1-benzyl group (i.e., 21b) produced 25 000-fold selectivity for β2-AR. Moreover, the type (or the lack) of substitution at the 4-position may be key to the design of β2-AR selective ligands based on the parent drug, trimetoquinol (1). Experimental Section Chemistry. Melting points were determined on a ThomasHoover capillary melting point apparatus and are uncorrected. Infrared spectra were recorded on a Perkin Elmer System 2000 FT-IR. Proton and carbon-13 magnetic resonance spectra were obtained on a Bruker AX 300 spectrometer (300 and 75 MHz for 1H and 13C, respectively). Chemical shift values are reported as parts per million (δ) relative to tetramethylsilane (TMS). Spectral data are consistent with assigned structures. Elemental analyses were performed by Atlantic Microlab Inc., Norcross, GA, and found values are within 0.4% of the theoretical values. Routine thin-layer chromatography (TLC) was performed on silica gel GHlF plates (250 m, 2.5 × 10 cm; Analtech Inc., Newark, DE). Flash chromatography was performed on silica gel (Merck, grade 60, 230-400 mesh, 60 Å). Tetrahydrofuran (THF) was dried by distillation from sodium metal, and acetonitrile (MeCN), CHCl3, and methylene chloride (CH2Cl2) were dried by distillation from P2O5. All anhydrous solvents (except anhydrous Et2O and THF) were stored over 3- or 4-Å molecular sieves. N-(3,4-Dimethoxyphenethyl)-4-nitrophenylacetamide (5a). A solution of 3,4-dimethoxyphenethylamine (5.0 g, 27.6 mmol) and 4-nitrophenylacetic acid (7.5 g, 41.4 mmol) in toluene (150 mL) was heated at reflux for 72 h in a flask equipped with a Dean-Stark trap under an argon atmosphere. The solvent was evaporated in vacuo, and the residue was taken up in CH2Cl2 (200 mL). The solution was washed consecutively with H2O (100 mL), 10% HCl (2 × 100 mL), H2O (2 × 100 mL), 10% NaHCO3 (2 × 200 mL), and H2O (2 × 100 mL) and dried over MgSO4. The solvent was evaporated, and the crude solid was recrystallized from EtOAc to give 5.49 g (58%) of the product as ivory-colored needles: mp 119-121 °C (lit.22 130-132 °C, ethanol-2-propanol); 1H NMR (CDCl3) δ 8.16 (d, J ) 8.8 Hz, 2H, ArH), 7.37 (d, J ) 8.8 Hz, 2H, ArH), 6.73 (d, J ) 8.1 Hz, 1H, ArH), 6.65 (d, J ) 1.9 Hz, 1H, ArH), 6.60 (dd, J ) 8.1 & 1.9 Hz, 1H, ArH), 5.40 (m, 1H, NH), 3.86 (s, 3H, OMe), 3.84 (s, 3H, OMe), 3.59 (s, 2H, CH2), 3.51 (q, J ) 6.9 Hz, 2H, CH2), 2.73 (t, J ) 6.9 Hz, CH2); IR (KBr) 3320 (NH), 1650 (CdO) cm-1. Anal. (C18H20N2O5) C, H, N. N-(3,4-Dimethoxyphenethyl)-3,5-bis(trifluoromethyl)phenylacetamide (5c). A solution of 3,4-dimethoxyphenethylamine (2.72 g, 15 mmol) and 3,5-bis(trifluoromethyl)phenylacetic acid (2.72 g, 10 mmol) in toluene (50 mL) was heated at reflux for 80 h in a flask equipped with a DeanStark trap. The solvent was evaporated in vacuo, and the

Iodinated Analogs of Trimetoquinol

Journal of Medicinal Chemistry, 1996, Vol. 39, No. 19 3707

6,7-Bis(benzyloxy)-2-(tert-butoxycarbonyl)-1-(4-nitrobenzyl)-1,2,3,4-tetrahydroisoquinoline (7b). A solution of (Boc)2O (2.84 g, 13 mmol) in THF (10 mL) was added to a cold mixture (ice bath) of isoquinoline 6b (6.20 g, 12 mmol) in THF (100 mL) and 1 N NaOH solution (30 mL). The ice bath was removed, and stirring was continued at room temperature overnight. THF was evaporated under reduced pressure, water was added, and the product was extracted with CH2Cl2, dried over MgSO4, filtered, and evaporated again. The oily residue was dissolved in ether and put in a refrigerator. Pink crystals were filtered and washed with ether to give 6.00 g (86%) of the title compound: mp 150-152 °C; 1H NMR (CDCl3) δ (the spectrum consists of two rotamers of 5:4 ratio) 8.11 and 8.06 (d, J ) 8.2 Hz, 2H, ArH), 7.47-7.27 and 7.217.11 (m, 12H, ArH), 6.70 and 6.67 (s, 1H, H-5), 6.56 and 6.44 (s, 1H, ArH), 5.27-4.96 (m, 5H, CH2O + CH), 4.12 and 3.74 (m, 1H, CH), 3.25-3.00 (m, 3H, CH, CH2Ar), 2.87-2.60 (m, 1H, CH), 2.57-2.37 (m, 1H, CH), 1.38 and 1.25 (s, 9H, t-Bu); IR (KBr) 1688 (CdO), 1518, 1345 (NO2) cm-1. Anal. (C35H36N2O6) C, H, N. 1-(4-Aminobenzyl)-6,7-dimethoxy-2-(trifluoroacetyl)1,2,3,4-tetrahydroisoquinoline (8a). A solution of 7a (5.20 g, 12.24 mmol) in ethyl acetate (200 mL) was hydrogenated (60 psi) over 5% Pd/C (1 g) for 2 h. The catalyst was removed by filtration, and the filtrate was evaporated to dryness to give a beige solid. Recrystallization from ethyl acetate and hexane gave 4.20 g (87%) of the product as light pink to white crystals: mp 157-160 °C; 1H NMR (CDCl3) δ 6.88 (d, 2H, ArH), 6.59 (d, 3H, ArH), 6.32 (s, 1H, ArH), 5.53 (t, 1H, ArCHN), 3.99 (m, 1H, CH), 3.86 (s, 3H, OMe), 3.71 (s, 3H, OMe), 3.60 (bs, 2H, NH2), 3.42-3.56 (m, 2H, CH), 2.85-3.20 (m, 3H, CH), 2.59-2.73 (m, 1H, CH); IR (KBr) 3370 (m, NH2), 1689 (CdO) cm-1; MS m/e (M+) 395 (M+H, FAB). Anal. (C20H21F3N2O3) C, H, N. 1-(4-Aminobenzyl)-6,7-bis(benzyloxy)-2-(tert-butoxycarbonyl)-1,2,3,4-tetrahydroisoquinoline (8b). The nitro compound 7b (6.00 g, 10.3 mmol) was dissolved in EtOAc (230 mL) in a Parr bottle. The solution was charged with a slurry of Raney-Ni (4 mL) and hydrogenated at 50 psi for 3 h. The solution was filtered through Celite and evaporated to give 5.10 g (90%) of the crude compound. The product was purified by flash chromatography (silica gel, hexane-EtOAc, 2:1) to give a foamy glassy solid (4.51 g, 71%); 1H NMR (CDCl3) δ (the spectrum consists of two rotamers of 5:2 ratio) 7.48-7.24 (m, 10H, 2×Ph), 6.82 (m, J ) 8.2 Hz, ArH), 6.68 and 6.64 (s, 1H, ArH), 6.58 (m, J ) 8.2 Hz, 2H, ArH), 6.49 and 6.32 (s, 1H, ArH), 5.12-4.81 (m, 5H, 2×CH2O + CH), 4.18-4.08 and 3.81-3.71 (m, 1H, CH), 3.27-3.09 (m, 1H, CH), 3.00-2.60 (m, 3H, CH2Ar, CH), 2.59-2.40 (m, 1H, CH), 1.43 and 1.32 (s, 9H, t-Bu); IR (KBr) 3451 and 3365 (NH2), 1684 (CdO), 1624 (NH bend), 1517 (CdC Ar) cm-1. Anal. (C35H38N2O4) C, H, N. 1-(4-Amino-3-iodobenzyl)-6,7-dimethoxy-2-(trifluoroacetyl)-1,2,3,4-tetrahydroisoquinoline (9a). A mixture of isoquinoline 8a (6.26 g, 18 mmol), benzyltrimethylammonium dichloroiodate (BTMAICl2) (8.15 g 18 mmol), and CaCO3 (2.30 g, 23 mmol) in CH2Cl2 (200 mL) and absolute MeOH (80 mL) was stirred overnight at room temperature; then BTMAICl2 (0.31 g, 0.9 mmol) was added and stirred for 1 h. The solution was filtered, washed with Na2S2O3 solution and water, dried over MgSO4, and concentrated under reduced pressure. Recrystallization of the residue from AcOEt gave the title compound (7.90 g, 84%): mp 198-200 °C (dec); 1H NMR (CDCl3) δ 7.34 (d, J ) 2.0 Hz, 1H, ArH), 6.90 (dd, J ) 2.0, 8.1 Hz, 1H, ArH), 6.66 (d, J ) 8.1 Hz, 1H, ArH), 6.59 (s, 1H, ArH), 6.29 (s, 1H, ArH), 5.48 (t, J ) 6.6 Hz, 1H, CH), 4.03 (s, 2H, NH2), 3.93 (m, 1H, CH), 3.86 (s, 3H, OMe), 3.71 (s, 3H, OMe), 3.53 (ddd, J ) 14.3, 10.8, 4.0 Hz, 1H, CH), 2.96 (m, 2H, CH2Ar), 2.90 (ddd, J ) 15.9, 10.6, 5.6 Hz, 1H, CH), 2.68 (dt, J ) 16.2, 4.2 Hz, 1H, CH); 13C NMR (CDCl3) δ 155.75 (q), 148.10, 147.41, 145.63, 139.77, 130.64, 128.46, 126.45, 124.86, 116.47 (q), 114.51, 110.96, 110.30, 83.64, 55.86, 55.79, 55.48, 40.41, 28.52; IR (KBr) 3435 and 3344 (NH2), 1693 (CdO), 1627 (NH bend), 1521 and 1501 (CdC Ar) cm-1. Anal. (C20H20F3IN2O3) C, H, N. 1-(4-Amino-3-iodobenzyl)-6,7-bis(benzyloxy)-2-(tert-butoxycarbonyl)-1,2,3,4-tetrahydroisoquinoline (9b). A mix-

ture of isoquinoline 8b (1.11 g, 3.2 mmol), BTMACl2I (1.1 g, 3.2 mmol), and CaCO3 (0.44 g, 4.4 mmol) in CH2Cl2 (50 mL) and MeOH (20 mL) was stirred overnight at room temperature. CaCO3 was filtered and washed with CH2Cl2. The filtrate was washed with a solution of Na2S2O3 (×2) and H2O (×2), dissolved in CHCl3 and EtOH, and concentrated till the beginning of crystallization to give 1.59 g (81%) of title compound as pink crystals: mp 169-171 °C; 1H NMR (CDCl3) δ (the spectrum consists of two rotamers of 2:1 ratio) 7.477.25 (m, 11H, ArH), 6.81 (dd, J ) 8.1, 1.6 Hz, 1H, ArH), 6.706.59 (m, 2H, ArH), 6.49 and 6.30 (s, 1H, ArH), 5.20-5.85 (m, 5H, CH2O + CH), 4.13 and 3.74 (m, 1H, CH), 4.01 (s, NH2), 3.30-3.10 (m, 1H, CH), 2.96-2.59 (m, 3H, CH2Ar + CH), 2.59-2.43 (m, 1H, CH), 1.44 and 1.32 (s, 9H, t-Bu); IR (KBr) 3453 and 3334 (NH2), 1667 (CdO), 1627 (NH bend), 1520 and 1498 (CdC Ar) cm-1. Anal. (C35H37IN2O4) C, H, N. 1-(4-Amino-3,5-diiodobenzyl)-2-(trifluoroacetyl)-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline (10a). To a solution of 8a (0.1 g, 2.54 mmol) in CH2Cl2 (50 mL) and methanol (20 mL) was added BTMACl2I (2.0 g, 5.77 mmol) and CaCO3 (2.0 g). The mixture was stirred overnight at room temperature. A second portion of BTMACl2I (0.97 g, 2.8 mmol) was added, and stirring was continued overnight. Analysis of the reaction indicated a mixture of mono- and diiodinated products. The reaction mixture was filtered. The filtrate was washed consecutively with aqueous 5% Na2S2O3 (40 mL) and water (50 mL), and then dried with Na2SO4. Evaporation of the solvent gave a reddish glassy solid. The desired diiodinated product was purified from the crude mixture by flash chromatography (CH2Cl2-EtOAc, 9:1). The appropriate fractions were combined and evaporated in vacuo to give 0.72 g (44%) of the product as a white solid: mp 183-184.5 °C; 1H NMR (CDCl3) δ 7.37 (s, 2H, ArH), 6.61 (s, 1H, ArH), 6.29 (s, 1H, ArH), 5.43 (m, 1H, ArH), 4.56 (bs, 2H, NH2), 3.87 (s, 3H, OMe), 3.73 (s, 3H, OMe), 3.60 (m, 1H, CH), 2.91 (m, 4H, CH), 2.70 (m, 1H, CH); IR (KBr) 3429, 3348 (NH), 1685 (CdO) cm-1. Anal. (C20H19F3I2N2O3) C, H, N. 4′,4′′-Azobis[1-(4-amino-3,5-diiodobenzyl)-6,7-dimethoxy2-(trifluoroacetyl)-1,2,3,4-tetrahydroisoquinoline] (11) was isolated from the above mixture as a bottom spot, deep purple solid: mp 229-232 °C; 1H NMR (CDCl3) δ 7.78 (s, 2H, ArH), 6.65 (s, 1H, ArH), 6.18 (s, 1H, ArH), 5.53 (dd, J ) 7.9, 5.6 Hz, 1H, ArH), 3.99 (m, 1H, CH), 3.88 (s, 3H, OMe), 3.74 (m, 1H, CH), 3.72 (s, 3H, OMe), 3.16 (dd, J ) 13.0, 5.3 Hz, 1H, CH), 2.96 (m, 2H, CH), 2.80 (dt, J ) 16.2, 4.5 Hz, 1H, CH); 13C NMR (CDCl3) δ 156.14 (q), 148.89, 148.54, 147.59, 142.27, 141.53, 125.60, 125.05, 116.47 (q), 111.28, 110.42, 89.83, 56.15, 56.01, 55.54, 40.88 (q), 40.60, 28.45; IR (KBr) 1688 (CdO), 1520 (CdC Ar) cm-1. Anal. (C40H34F6I4N4O6) C, H, N. The same product was obtained via diazotization of 10a (0.32 g, 0.5 mmol, see below) and stirring overnight with 20 mL of 6% H2SO3 at room temperature, yield 0.03 g (10%) after flash column chromatography. 4′,4′′-Azobis[1-(4-amino-3,5-diiodobenzyl)-6,7-dimethoxy1,2,3,4-tetrahydroisoquinoline] (12). A solution of azo compound 11 (0.26 g, 0.2 mmol) in 35 mL of MeOH and 0.85 g of K2CO3 in 11 mL was refluxed for 4 h and evaporated. Flash chromatography on silica gel (CH2Cl2, CH2Cl2-MeOH, 50:1, 30:1) gave 0.15 g (70%) of the product: mp 176-177 °C (dec); 1H NMR (300 MHz, CDCl ) δ 7.96 (s, 2H, ArH), 6.62 (s, 1H, 3 ArH), 6.61 (s, 1H, ArH), 4.20 (dd, J ) 9.6, 4.0 Hz, 1H, ArH), 3.87 (s, 3H, OMe), 3.86 (s, 3H, OMe), 3.10-3.30 (m, 2H, CH), 3.00 (m, 1H, CH), 2.68-2.90 (m, 2H, CH); 13C NMR (CDCl3) δ 148.43, 147.75, 147.21, 144.05, 141.87, 129.76, 127.43, 112.03, 109.34, 90.43, 56.61, 56.16, 55.88, 41.65, 40.51, 29.36; IR (KBr) 1515 (CdC, Ar) cm-1. Anal. (C36H36I4N4O4) C, H, N. 1-(4-Acetamido-3,5-diiodobenzyl)-6,7-dimethoxy-2-(trifluoroacetyl)-1,2,3,4-tetrahydroisoquinoline (13). A solution of acetyl chloride (0.80 g, 7.8 mmol) in dry THF (2 mL) was added dropwise to a stirred solution of 10a (1.0 g, 1.56 mmol), Et3N (0.40 g, 7.8 mmol), and (N,N-dimethylamino)pyridine (DMAP, at 10 mg) in dry THF (20 mL) at 0 °C under an argon atmosphere. After the addition, the reaction mixture was allowed to warm to room temperature, and stirring was continued overnight (14 h). The reaction was quenched with

3708

Journal of Medicinal Chemistry, 1996, Vol. 39, No. 19

De Los Angeles et al.

H2O (20 mL) and stirred for 30 min. The solution was extracted with Et2OAc (3 × 75 mL). The organic extract was washed with H2O (20 mL), dried (Na2SO4), and evaporated in vacuo to give a tan solid. Recrystallization of the crude product from EtOH and H2O gave 0.93 g (87%) of the title compound as light beige needles: mp 218-219 °C; 1H NMR (CDCl3) δ 7.5-7.75 (bm, 2H, ArH), 6.96 (s, 1H, CONH), 6.62 (s, 1H, ArH), 6.24 (s, 1H, ArH), 5.47 (t, J ) 6.7 Hz, 1H, CH), 3.98 (m, 1H, CH), 3.87 (s, 3H, OMe), 3.73 (s, 3H, OMe), 2.83-3.18 (m, 4H, CH), 2.74 (m, 1H, CH), 2.22 (s, 3H, Ac); 13C NMR (CDCl3) δ 168.14, 156.02 (q), 148.39, 147.68, 140.58, 140.31, 139.35, 125.71, 124.85, 116.4 (q), 98.73, 56.09, 55.93, 55.44, 40.63 (q), 40.48, 28.43, 23.62; IR (KBr) 3387 (NH), 1683 (CO). Anal. (C22H21F3I2N2O4) C, H, N. 1-(4-Acetamido-3,5-diiodobenzyl)-6,7-dimethoxy-1,2,3,4tetrahydroisoquinoline Hydrochloride (14‚HCl). A solution of 13 (1.22 g, 1.78 mmol) in methanol (60 mL) was added to a solution of K2CO3 (5.6 g) in 80 mL of 1:1 methanol and water. The mixture was stirred at room temperature for 3 h. The resulting solution was concentrated and then extracted with ethyl acetate (3 × 80 mL). The organic solution was dried (Na2SO4) and evaporated in vacuo to give 0.79 g (75%) of the product as the free base. The free base converted to the hydrochloride salt and recrystallized from anhydrous ethanol and ethyl ether: mp 196-200 °C (dec); 1H NMR (DMSO-d6) δ 9.85 (s, 1H, CONH), 9.35 (bm, 2H, NH+), 7.98 (s, 1H, ArH), 7.96 (s, 1H, ArH), 6.78 (s, 1H, ArH), 6.65 (s, 1H, ArH), 4.65 (bm, 1H, CH), 3.83 (s, 3H, OMe), 3.73 (s, 3H, OMe), 3.353.43 (m, 1H, CH), 2.8-3.2 (m, 5H, CH), 2.01 (s, 3H, Me); IR (KBr) 1677 (CdO), 1514 (CdC Ar) cm-1; MS m/e (M+) 592 (MHCl, EI). Anal. (C20H22I2N2O3‚HCl‚0.5Et2O) C, H, N. 1-(4-Acetamido-3,5-diiodobenzyl)-6,7-dihydroxy-1,2,3,4tetrahydroisoquinoline Hydrobromide (15‚HBr). To a solution of 14 (0.50 g, 0.88 mmol) in dry CH2Cl2 (50 mL) at 0 °C (ice bath) was added dropwise 1 M BBr3 (4 mL, 4 mmol) in CH2Cl2 under an argon atmosphere. The mixture was then allowed to reach room temperature, and stirring was continued overnight. The reaction mixture was cooled with an ice bath, and methanol (20 mL) was added carefully. The solution was stirred for 10 min and then evaporated in vacuo. This was repeated 4 times to give a solid which was stirred with ether overnight. The crude product was collected by filtration and recrystallized from methanol and ethyl ether to give 0.51 g (90%) of the desired product as an off-white solid: mp 202206 °C (dec); 1H NMR (DMSO-d6) δ 9.82 (s, 1H, CONH), 9.15 (bm, 1H, OH), 8.91 (bm, 2H, NH+), 8.55 (bm, 1H, OH), 7.97 (s, 1H, ArH), 7.94 (s, 1H, ArH), 6.71 (s, 1H, ArH), 6.56 (s, 1H, ArH), 4.66 (bm, 1H, CH), 3.27-3.35 (m, 2H, CH), 3.10-3.16 (m, 2H, CH), 2.70-2.93 (m, 4H, CH), 2.02 (s, 3H, Me); 13C NMR (CD3OD) δ 172.51 (CdO), 147.04 and 145.89 (C-6 and C-7), 142.34 (C-4′), 141.90 and 141.73 (C-2′ and C-6′), 140.18 (C-1′), 123.67 and 123.09 (C-5a and C-8a), 116.32 (C-5), 114.11 (C8), 100.61 and 100.46 (C-3′ and C-5′), 57.51 (C-1), 41.10 (C-3), 39.31 (CH2Ar), 25.70 (C-4), 23.09 (COCH3); IR (KBr) 1652 (CO), 1524 (C-N) cm-1; MS m/e (M+) 565 (M+H, FAB). Anal. (C18H18N2O3I2‚HBr‚0.25Et2O) C, H, N. 1-(4-Diacetamido-3,5-diiodobenzyl)-6,7-dimethoxy-2(trifluoroacetyl)-1,2,3,4-tetrahydroisoquinoline (16). A solution of 10a (0.70 g, 1.08 mmol) in acetic anhydride (10 mL) was heated at reflux for 2 h. The solvent was evaporated in vacuo to give an oily residue. The residue was taken up in hot ethanol. The product crystallized upon cooling to give 0.78 g (99%) of the product as white crystals: mp 190-192 °C; 1H NMR (CDCl3) δ 7.70 (s, 2H, ArH), 6.64 (s, 1H, ArH), 6.39 (s, 1H, ArH), 5.53 (t, J ) 6.7 Hz, 1H, CH), 3.96 (m, 1H, CH), 3.87 (s, 3H, OMe), 3.79 (s, 3H, OMe), 2.68 (m, 1H, CH), 2.94-3.07 (m, 3H, CH), 2.77 (m, 1H, CH), 2.28 (s, 6H, Ac); 13C NMR (CDCl3) δ 171.23, 155.93 (q), 148.48, 147.86, 142.66, 141.36, 141.12, 125.65, 124.83, 116.31 (q), 111.14, 109.88, 99.21, 56.08, 55.93, 55.31, 40.58, 40.44 (q), 28.43, 26.60; IR (KBr) 1719, 1683 (CdO), 1235, 1207 (C-O) cm-1. Anal. (C24H23F3I2N2O5) C, H, N. 6,7-Dihydroxy-1-(4-hydroxy-4,3,5-diiodobenzyl)-1,2,3,4tetrahydroisoquinoline Hydrobromide (18‚HBr). Hydrochloride 1710 (0.21 g, 0.28 mmol) was dissolved in CHCl3 and washed with 1 N NaOH; the organic layer was separated,

washed with water, and dried over MgSO4. The solution was filtered, evaporated, and dried under vacuum. The residue was dissolved in CH2Cl2 (4 mL), and 1 M BBr3 in CH2Cl2 (1.39 mL, 1.39 mmol) was added at -78 °C under an argon atmosphere. The mixture was stirred overnight at room temperature followed by addition of MeOH (1 mL) and stirring for 5 h. The resulting solution was evaporated with MeOH 5 times, and the residue was recrystallized from MeOH-ether to give 0.078 g (45%) of white crystals: mp 235-237 °C (dec); 1 H NMR (DMSO-d6) δ 9.50 (s, 1H, OH), 9.14 (s, 1H, OH), 8.89 (s, 1H, OH), 8.78 (br s, 1H, NH+), 8.43 (br s, 1H, NH+), 7.76 (s, 2H, ArH), 6.64 (s, 1H, ArH), 6.55 (s, 1H, ArH), 4.55 (m, 1H, CH), 3.40-3.05 (m, 3H, CH), 2.92-2.68 (m, 3H, CH); 13C NMR (CD3OD) δ 156.59 (C-4′), 146.96 and 145.85 (C-6 and C-7), 141.69 (C-2′ and C-6′), 132.30 (C-1′), 123.71 and 123.24 (C-4a and C-8a), 116.25 (C-5), 114.14 (C-8), 85.85 (C-3′ and C-5′), 57.55 (C-1), 41.08 (C-3), 39.12 (CH2Ar), 25.68 (C-4); IR (KBr) 3600-2600 (OH, NH), 1527 (CdC, Ar) cm-1. Anal. (C16H15I2NO3‚HBr‚0.1Et2O) C, H, N. 1-(3,5-Diiodobenzyl)-6,7-dimethoxy-2-(trifluoroacetyl)1,2,3,4-tetrahydroisoquinoline (19a). A solution of isoquinoline 10a (1.29 g, 2 mmol) in glacial acetic acid (30 mL) was added to a cold solution of NaNO2 (0.19 g, 2.8 mmol) in concentrated (d 1.84) H2SO4 (3.4 mL); the temperature was kept within 0-5 °C. The solution was poured into ice-water (60 g), and H3PO2 (12 mL) was added in 30 min. The cooling bath was removed, and the solution was allowed to stand at room temperature for 2 days. The precipitate was filtered, dried, and chromatographed on silica gel (hexane-AcOEt, 8:1). Recrystallization from AcOEt-hexane gave 0.50 g (40%) of white crystals: mp 162-163 °C; 1H NMR (CDCl3) δ 7.93 (t, J ) 1.5 Hz, 1H, ArH), 7.43 (d, J ) 1.5 Hz, 2H, ArH), 6.62 (s, 1H, ArH), 6.24 (s, 1H, ArH), 5.48 (t, J ) 6.7 Hz, 1H, CH), 3.95 (m, 1H, CH), 3.87 (s, 3H, OMe), 3.72 (s, 3H, OMe), 3.61 (m, 1H, CH), 3.06-2.86 (m, 3H, CH), 2.70 (m, 1H, CH); 13C NMR (CDCl3) δ 155.98 (q), 148.46, 147.67, 143.60, 141.25, 137.98, 125.86, 125.00, 116.41 (q), 111.20, 110.19, 94.58, 55.96, 55.93, 55.37, 41.13, 40.61 (q), 28.44; IR (KBr) 1686 (CdO), 1541, 1520 (CdC Ar) cm-1. Anal. (C18H19I2NO2) C, H, N. 1-(3,4,5-Triiodobenzyl)-6,7-dimethoxy-2-(trifluoroacetyl)-1,2,3,4-tetrahydroisoquinoline (19b). Compound 10a (1.29 g, 2 mmol) was diazotized in the usual manner. The resulting solution was poured in ice-water (60 g) followed by addition of KI (0.47 g, 2.8 mmol) in water (10 mL). The mixture was heated to 80 °C and allowed to cool. The precipitate was filtered, dried, and purified by column chromatography (silica gel, hexane-AcOEt, 3:1): yield 0.51 g (34%); mp 215-216 °C; 1H NMR (CDCl3) δ 7.59 (s, 2H, ArH), 6.63 (s, 1H, ArH), 6.31 (s, 1H, ArH), 5.46 (t, J ) 6.7 Hz, 1H, ArH), 3.93 (m, 1H, CH), 3.88 (s, 3H, OMe), 3.75 (s, 3H, OMe), 3.61 (ddd, J ) 13.8, 9.9, 4.1 Hz, 1H, CH), 3.02-2.85 (m, 3H, CH2Ar + CH), 2.70 (dt, J ) 16.2, 4.3 Hz, 1H, CH); 13C NMR (CDCl3) δ 156.07 (q), 148.48, 147.71, 140.45, 139.90, 125.67, 125.08, 118.91, 116.39 (q), 111.16, 110.08, 106.78, 55.97, 55.10, 40.68 (q), 40.32 28.43; IR (KBr) 1685 (CdO), 1519 (CdC Ar) cm-1. Anal. (C20H17F3I3NO3) C, H, N. 1-(3,5-Diiodobenzyl)-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline (20a). A mixture of isoquinoline 19a (0.38 g, 0.6 mmol) in MeOH (35 mL) and K2CO3 (0.85 g) in water (11 mL) was refluxed for 1.5 h. MeOH was evaporated, and a white precipitate was filtered and dried. The crude product was purified by flash chromatography (silica gel) using a gradient (EtOAc-hexanes, 1:2; EtOAc, EtOAc-MeOH, 30:1) and recrystallized from EtOAc-hexanes to give 0.25 g (76%) of white crystals: mp 122-124 °C; 1H NMR (CDCl3) δ 7.94 (t, J ) 1.4 Hz, 1H, ArH), 7.60 (d, J ) 1.4 Hz, 2H, ArH), 6.60 (s, 1H, ArH), 6.57 (s, 1H, ArH), 4.10 (m, 1H, CH), 3.86 (s, 3H, OMe), 3.84 (s, 3H, OMe), 3.18 (m, 1H, CH), 3.08 (dd, J ) 4.1 and 13.7 Hz, 1H, CH), 2.95 (m, 1H, CH), 2.82-2.61 (m, 3H, CH); 13C NMR (CDCl3) δ 147.68, 147.17, 143.78, 143.15, 137.64, 129.86, 127.42, 111.98, 109.32, 94.99, 56.57, 56.06, 55.87, 42.20, 40.55, 29.39; IR (KBr) 3325 (NH), 1516 (CdC Ar) cm-1. Anal. (C18H19I2NO2) C, H, N. 6,7-Dimethoxy-1-(3,4,5-triiodobenzyl)-1,2,3,4-tetrahydroisoquinoline (20b). A mixture of isoquinoline 19b (0.454 g, 0.6 mmol) in MeOH (35 mL) and K2CO3 in water (11 mL)

Iodinated Analogs of Trimetoquinol

Journal of Medicinal Chemistry, 1996, Vol. 39, No. 19 3709

was refluxed for 1.5 h. MeOH was evaporated, and a white precipitate was filtered and dried. Recrystallization from CHCl3-hexane gave 0.300 g (76%) of white crystals: mp 168170 °C (dec); 1H NMR (CDCl3) δ 7.79 (s, 2H, ArH), 6.60 (s, 1H, ArH), 6.58 (s, 1H, ArH), 4.09 (dd, J ) 9.8, 3.8 Hz, 1H, CH), 3.86 (s, 3H, OMe), 3.85 (s, 3H, OMe), 3.17 (m, 1H, CH), 3.04-2.88 (m, 2H, CH), 2.82-2.60 (m, 3H, CH); 13C NMR (CDCl3) δ 147.66, 147.15, 143.00, 139.68, 129.67, 127.42, 118.18, 111.92, 109.14, 107.09, 56.35, 56.06, 55.85, 41.38, 40.56, 29.35; IR (KBr) 3312 (NH), 1516 (CdC Ar) cm-1. Anal. (C18H18I3NO2) C, H, N. 1-(4-Amino-3,5-diiodobenzyl)-6,7-dimethoxy-1,2,3,4-tetrahydroisoquinoline (20c). A slurry of the isoquinoline 10a (1.94 g, 3 mmol) in MeOH (260 mL) and K2CO3 (11.2 g) in H2O (80 mL) was refluxed for 1 h. MeOH was evaporated under reduced pressure, and crystals were filtered, dried, and recrystallized from EtOAc-hexane to give the title compound (1.39 g, 84%): mp 169-171 °C; 1H NMR (CDCl3) δ 7.55 (s, 2H, ArH), 6.61 (s, 1H, ArH), 6.51 (s, 1H, ArH), 4.54 (s, 2H, NH2), 4.04 (dd, J ) 9.6, 4.0 Hz, 1H, CH), 3.86 (s, 3H, OMe), 3.84 (s, 3H, OMe), 3.18 (m, 1H, CH), 3.03 (dd, J ) 13.8, 4.0 Hz, 1H, CH), 2.92 (ddd, J ) 12.1, 6.8, 5.2 Hz, 1H, CH), 2.822.61 (m, J ) 13.8, 9.6 Hz, 3H, CH); 13C NMR (CDCl3) δ 147.51, 147.05, 144.66, 139.97, 132.36, 130.14, 127.38, 111.88, 109.28, 81.59, 56.79, 56.01, 55.83, 40.87, 40.72, 29.47; IR (KBr) 3416 (NH), 3331 (NH), 1607 (NH bend), 1571, 1512 (CdC Ar) cm-1. Anal. (C18H20N2I2) C, H, N. 6,7-Dihydroxy-1-(3,5-diiodobenzyl)-1,2,3,4-tetrahydroisoquinoline Hydrobromide (21a‚HBr). The isoquinoline 20a (0.19 g, 0.35 mmol) was demethylated using the same procedure as 15. Recrystallization from MeOH-ether gave 0.20 g (96%) of the title compound: mp 157-159 °C (dec); 1H NMR (DMSO-d6) δ 9.13 (bs, 1H, OH), 8.88 (bm, 2H, NH+OH), 8.57 (bm, 1H, NH), 8.02 (t, J ) 1.4 Hz, 1H, ArH), 7.80 (d, J ) 1.4 Hz, 2H, ArH), 6.61 (s, 1H, ArH), 6.56 (s, 1H, ArH), 4.63 (bm, 1H, CH), 3.22-3.41 (m, 2H, CH), 3.14 (m, 1H, CH), 2.712.96 (m, 3H, CH); 13C NMR (CD3OD) δ 147.01 and 145.79 (C-6 and C-7), 145.69 (C-4′), 139.18 (C-2′ and C-6′), 141.15 (C-1′), 123.76 and 123.03 (C-4a and C-8a), 116.30 (C-5), 114.21 (C8), 96.14 (C-3′ and C-5′), 57.25 (C-1), 41.02 (C-3), 40.08 (CH2Ar), 25.60 (C-4); IR (KBr) 3600-2700 (br OH, NH), 1617, 1521 (CdC Ar) cm-1. Anal. (C16H15BrI3NO2) C, H, N. 6,7-Dihydroxy-1-(3,4,5-triiodobenzyl)-1,2,3,4-tetrahydroisoquinoline Hydrobromide (21b‚HBr). The isoquinoline 20b (0.23 g, 0.35 mmol) was demethylated using the same procedure as 15. Recrystallization from MeOH-ether gave 0.24 g (97%) of the title compound: mp 210-213 °C (dec); 1H NMR (MeOH-d4) δ 7.92 (s, 2H, ArH), 6.63 (s, 1H, ArH), 6.56 (s, 1H, ArH), 4.64 (dd, J ) 5.7 and 3.1 Hz, 1H, CH), 3.423.53 (m, 1H, CH), 3.2-3.34 (m, 2H, CH), 2.83-3.07 (m, 3H, CH); 13C NMR (CD3OD) δ 147.11 and 145.90 (C-6 and C-7), 141.06 (C-2′ and C-6′), 140.21 (C-1′), 123.70 and 122.98 (C-4a and C-8a), 120.90 (C-4′), 116.31 (C-5), 114.14 (C-8), 108.68 (C3′ and C-5′), 57.04 (C-1), 41.01 (C-3), 39.38 (CH2Ar), 25.62 (C4); IR (KBr) 3600-2700 (br OH, NH), 1617, 1540 (CdC Ar) cm-1. Anal. (C16H16BrI2NO2) C, H, N. 1-(4-Amino-3,5-diiodobenzyl)-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline Dihydrochloride (21c‚2HCl). The isoquinoline 20c (1.21 g, 2.2 mmol) was demethylated in the same manner as 20b to give 1.14 g (76%) of the dihydrobromide salt: mp 155-157 °C (dec). The product was dissolved in MeOH, chromatographed (silica gel, EtOAc-NH4OH, 100: 1), and evaporated with EtOH (×5). To an ethanol solution was added a 1 N etherial solution of HCl (3 mL), concentrated, precipitated with EtOAc, and recrystallized from MeOH-i-PrOH: mp 176-178 °C (dec); 1H NMR (DMSO-d6) δ 9.15 (br s, 1H, OH), 8.89 (br s, 1H, NH2+), 7.68 (s, 2H, H-2′), 6.64 (s, 1H, H-5), 6.55 (s, 1H, H-8), 5.06 (br s, 2H, NH2), 4.47 (m, 1H, H-1), 3.40-2.67 (m, 6H, H-3 + H-4 + CH2Ar); 13C NMR (CD3OD) δ 147.99 (C-4′), 146.84 and 145.75 (C-6 and C-7), 141.60 (C-2′ and C-6′), 128.78 (C-1′), 123.75 and 123.33 (C-4a and C-8a), 116.24 (C-5), 114.16 (C-8), 81.86 (C-3′ and C-5′), 57.59 (C-1), 41.07 (C-3), 39.07 (CH2Ar), 25.68 (C-4); IR (KBr) 3600-2500 (br, OH, NH), 1607 (NH bend), 1529 (CdC Ar) cm-1. Anal. (C16H16I2N2O2‚2HCl‚H2O) C, H, N.

1-(4-Acetamido-3-iodobenzyl)-6,7-dimethoxy-2-(trifluoroacetyl)-1,2,3,4-tetrahydroisoquinoline (22). To a solution of isoquinoline 9a (0.52 g, 1 mmol) in hot benzene (15 mL) was added Ac2O (0.51 g, 5 mmol). The solution was refluxed for 1 h. The reaction mixture was allowed to cool. White crystals were filtered. Mother liquor was concentrated, and hexane was added. Slightly creamy crystals were filtered, total yield 0.53 g (94%). To get an analytical sample, the compound was recrystallized from EtOAc-hexane: mp 174175 °C; 1H NMR (CDCl3) δ 8.11 (d, J ) 8.3 Hz, 1H, H-5′), 7.52 (d, J ) 1.5 Hz, 1H, H-2′), 7.36 (s, 1H, NH), 7.10 (dd, J ) 8.3, 1.5 Hz, 1H, ArH), 6.60 (s, 1H, ArH), 6.32 (s, 1H, ArH), 5.23 (t, J ) 6.6 Hz, CH), 3.94 (m, 1H, CH), 3.87 (s, 3H, OMe), 3.72 (s, 3H, OMe), 3.54 (ddd, OMe ) 14.1, 10.4 Hz, 3.8 Hz, 1H, CH), 3.06 (m, 2H, CH), 2.90 (ddd, J ) 15.9, 10.4, 5.2 Hz, 1H, CH), 2.68 (dt, J ) 16.0, 4 Hz, 1H, CH), 2.23 (s, 3H, Ac); 13C NMR (CDCl3) δ 168.12, 155.90 (q), 148.30, 147.63, 139.63, 137.12, 134.95, 130.53, 126.19, 124.95, 121.73, 116.44 (q), 111.10, 110.17, 89.68, 55.91, 55.33, 40.75, 40.50 (q), 28.51, 24.75; IR (KBr) 3395 (NH), 1688 (CdO), 1519 (CdC Ar) cm-1. Anal. (C22H22F3IN2O4) C, H, N. 1-(4-Acetamido-3-iodobenzyl)-6,7-dimethoxy-1,2,3,4tetrahydroisoquinoline (23). The title compound (0.57 g, 69%) as a glassy solid was obtained from the isoquinoline 22 (0.99 g, 1.76 mmol) in the same manner as 20c: 1H NMR (CDCl3) δ 8.12 (d, J ) 8.3 Hz, 1H, H-5′), 7.70 (d, J ) 1.7 Hz, 1H, ArH), 7.38 (s, 1H, NH), 7.26 (dd, J ) 8.3, 1.7 Hz, 1H, ArH), 6.63 (s, 1H, ArH), 6.60 (s, 1H, ArH), 4.11 (dd, J ) 9.6, 3.8 Hz, 1H, CH), 3.10-3.25 (m, 2H, CH), 2.92 (m, 1H, CH), 2.63-2.86 (m, 3H, CH2Ar, CH), 2.25 (s, 3H, Ac); 13C NMR (CDCl3) δ 168.19, 147.54, 147.05, 139.31, 137.30, 136.66, 130.12, 129.92, 127.28, 122.25, 111.85, 109.27, 90.50, 56.64, 55.99, 55.79, 41.63, 40.61, 29.30, 24.66; IR (KBr) 3391 (NH), 1676 (CdO), 1515 (CdC Ar) cm-1. Anal. (C20H23IN2O3) C, H, N. 1-(4-Amino-3-iodobenzyl)-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline Dihydrochloride (24‚2HCl). The title compound was obtained in the same manner as 21c from the isoquinoline 23 (0.42 g, 0.90 mmol). The product was recrystallized from MeOH (twice) to give 0.32 g (64%). The compound was dissolved in NaHCO3 solution and extracted with EtOAc (×5). The solution was dried (MgSO4) and evaporated; 1 M HCl in ether (2 mL) was added to the methanol solution of the residue. The solution was concentrated and put in a refrigerator. The white crystals were filtered, washed with EtOAc, and dried: dec.p. 186-190 °C; 1H NMR (DMSO-d6) δ 9.05 (bs, 1H, OH), 7.68 (d, J ) 1.7 Hz, 1H, ArH), 7.16 (dd, J ) 8.2, 1.7 Hz, 1H, ArH), 6.94 (d, J ) 8.2 Hz, 1H, ArH), 6.57 (s, 1H, ArH), 6.55 (s, 1H, ArH), 4.46 (m, 1H, CH), 3.27 (m, 1H, CH), 3.00-3.20 (m, 2H, CH), 2.80-3.00 (m, 2H, CH), 2.74 (dt, J ) 16.8, 5.9 Hz, CH); 13C NMR (CD3OD) δ 147.00 and 145.76 (C-6 and C-7), 1412.68 (C-2′), 138.21 (C-4′), 136.27 (C-1′), 132.35 (C-6′), 123.82 and 123.10 (C-4a and C-8a), 116.27 (C5), 114.33 (C-8), 124.24 (C-5′), 91.49 (C-3′), 57.27 (C-1), 40.90 (C-3), 39.87 (CH2Ar), 25.659 (C-4); IR (KBr) 3600-2300 (br, OH, NH), 1607 (NH bend), 1526 (CdC Ar) cm-1. Anal. (C16H17IN2O2‚2HCl) C, H, N. 1-(4-Acetamido-3-iodobenzyl)-6,7-bis(benzyloxy)-2-(tertbutoxycarbonyl)-1,2,3,4-tetrahydroisoquinoline (25a). To a cold solution (0 °C) of isoquinoline 9b (0.68 g, 1 mmol) and Et3N (0.34 g, 3 mmol) in CH2Cl2 (10 mL) was added AcCl (0.16 g, 2 mmol). The cooling bath was removed, and the mixture was stirred overnight. The solution was washed with water (2×), dried over MgSO4, filtered, and evaporated. Ether was added and evaporated again to give a glassy solid (0.65 g, 90%): mp 62-64 °C; 1H NMR (CDCl3) δ (the spectrum consists of two rotamers of 5:3 ratio) 8.12 and 8.06 (d, J ) 8.2), 7.597.25 (m, 11H, ArH), 7.06 and 6.98 (m, 1H, ArH), 6.70 and 6.65 (s, 1H, ArH), 6.48 and 6.35 (s, 1H, ArH), 5.24-4.87 (m, 5H, CH2O + CH), 4.12 and 3.73 (m, 1H, CH), 3.27-3.11 (m, 1H, CH), 2.98-2.60 (m, 3H, CH2Ar + CH), 2.60-2.37 (m, 1H, CH), 2.22 (s, 3H, Ac), 1.43 and 1.31 (s, 9H, t-Bu); IR (KBr) 3389 (NH), 1688 (CdO), 1512 (CdC Ar) cm-1. Anal. (C37H39IN2O5) C, H, N. 1-(4-(Benzoylamino)-3-iodobenzyl)-6,7-bis(benzyloxy)2-(tert-butoxycarbonyl)-1,2,3,4-tetrahydroisoquinoline (25b). To a cold solution (0 °C) of isoquinoline 9b (0.68 g, 1

3710

Journal of Medicinal Chemistry, 1996, Vol. 39, No. 19

De Los Angeles et al.

mmol) and Et3N (0.30 g, 3 mmol) in 10 mL of CH2Cl2 was added benzoyl chloride (0.28 g, 2 mmol). The cooling bath was removed, and the mixture was stirred overnight. CH2Cl2 was added (30 mL), the solution was washed with water, dried over MgSO4, filtered, and evaporated till dryness. The oily residue was dissolved in ether and evaporated to give 0.60 g (76%) of a glassy solid. The compound was purified by column chromatography (silica gel, EtOAc-hexane 1:2): mp 151-153 °C; 1 H NMR (CDCl3) δ 8.42-6.36 (m, 18H, Ar), 5.20-4.90 (m, 5H, 2 × CH2O + H-1), 4.20-2.15 (m, 6H, aliphatic), 1.56-1.25 (m, 9H, t-Bu); IR (KBr) 3397 (NH), 1687 (CdO), 1513 (CdC Ar) cm-1. Anal. (C42H41IN2O5) C, H, N. 1-(4-Acetamido-3-iodobenzyl)-6,7-dihydroxy-1,2,3,4-tetrahydroisoquinoline Hydroiodide (26a‚HI). To a solution of isoquinoline 25a (0.40 g, 0.5 mmol) in anhydrous MeCN (5 mL) was added TMSI (0.40 g, 2 mmol) via a syringe in an argon atmosphere. The solution was stirred for 6 h followed by MeOH (1 mL) addition, and stirring was continued for 30 min. CH2Cl2 (30 mL) was added to the reaction mixture, and yellow crystals were filtered; yield 0.19 g (67%). The compound was dissolved in MeOH, AcOEt was added, and the solution was concentrated under reduced pressure. The crystals were filtered: dec.p. 172-174 °C; 1H NMR (DMSO-d6) δ 9.39 (s, 1H, NH), 8.86 (bs, 1H, OH), 8.50 (bs, 1H, OH), 7.90 (d, J ) 1.7 Hz, 1H, ArH), 7.41 (d, J ) 8.2 Hz, 1H, ArH), 7.35 (dd, J ) 8.2, 1.7 Hz, 1H, ArH), 6.63 (s, 1H, ArH), 6.56 (s, 1H, ArH), 4.63 (m, 1H, CH), 3.43-2.70 (m, 6H, CH), 2.06 (s, 3H, Ac); 13C NMR (CD3OD) δ 172.65 (CdO), 146.98 and 145.81 (C-6 and C-7), 142.68 (C-2′), 140.11 (C-4′), 137.15 (C-1′), 131.30 (C-6′), 129.10 (C-5′), 123.63 and 123.27 (C-4a and C-8a), 116.27 (C-5), 114.15 (C-8), 91.49 (C-3′), 57.60 (C-1), 41.01 (C-3), 39.98 (CH2Ar), 25.68 (C-4), 23.09 (COCH3); IR (KBr) 3600-2400 (br, OH, NH), 1655 (CdO), 1624 (NH bend), 1522 (CdC Ar) cm-1; MS m/z (M+) 439. Anal. (C18H19IN2O3‚HI‚0.25EtOAc) C, H, N. 1-(4-(Benzoylamino)-3-iodobenzyl)-6,7-dihydroxy-1,2,3,4tetrahydroisoquinoline Hydroiodide (26b‚HI). To a mixture of isoquinoline 25b (0.16 g, 2 mmol) in MeCN (4 mL) was added TMSI (0.16 g, 0.8 mmol) under argon atmosphere, and the solution was stirred at room temperature for 7 h. MeOH (1 mL) was added and stirred for 1 h followed by ether (40 mL) addition, and the yellow precipitate was filtered to give 0.10 g (80%) of the product. The compound was dissolved in MeOH, and EtOAc was added and concentrated until the beginning of crystallization: mp 185-188 °C (dec); 1H NMR (DMSO-d6) δ 9.98 (s, 1H, NHCOPh), 9.18 (s, 1H, NH), 8.88 (br, 2H, OH + NH), 8.54 (br, 1H, OH), 8.03-7.93 (m, 3H, ArH), 7.64-7.42 (m, 3H, ArH), 7.49 (d, J ) 8.1 Hz, 1H, ArH), 7.41 (dd, J ) 8.1, 1.4 Hz, 1H, ArH), 6.65 (s, 1H, ArH), 6.58 (s, 1H, ArH), 3.43-2.70 (m, 6H, CH); IR (KBr) 3500-2700 (br, NH, OH), 1649 (CdO), 1518 (CdC Ar) cm-1. Anal. (C23H21IN2O3‚ HI‚0.33EtOAc) C, H, N. 1-[3,5-Bis(trifluoromethyl)benzyl]-6,7-dihydroxy-1,2,3,4tetrahydroisoquinoline Hydrochloride (27‚HCl). The title compound was obtained from 6c in the same manner as 15 (85%). The product was converted to the hydrochloride salt, and recrystallization from methanol-ether gave the product as white crystals: mp 239-242 °C; 1H NMR (CD3OD-d4) δ 7.95 (s, 3H, ArH), 6.65 (s, 1H, ArH), 6.44 (s, 1H, ArH), 4.77 (t, J ) 7.7 Hz), 3.51 (dt, J ) 6.88, 12.74 Hz), 3.33 (m, 2H, CH), 3.0 (m, 2H, CH); 13C NMR (CD3OD) δ 147.15 and 145.81 (C-6 and C-7), 140.21 (C-1′), 133.21 (C-3′ and C-5′), 131.61 (C-2′ and C-6′), 124.79 (CF3), 123.79 and 122.63 (C-4a and C-8a), 122.58 (C-4′), 116.40 (C-5), 114.35 (C-8), 57.11 (C-1), 41.77 (C-3), 40.55 (CH2Ar), 25.56 (C-4); IR (KBr) 3600-2400 (N+H, OH), 1282 (C-O) cm-1. Anal. (C18H16ClF6NO2) C, H, N. Radioligand Binding Studies with β1- and β2-AR Expressed in CHO Cells. Competition binding experiments on β1- and β2-AR expressed in CHO cells were performed as described previously.8 CHO cells expressing human β1- and β2-AR (provided by A. D. Strosberg, Institut Cochin de Genetique Moleculaire, Paris, France, and David Bylund, University of Nebraska, Omaha, NE, respectively) were cultured in Ham’s F-12 medium supplemented with 10% fetal bovine serum, 50 units/mL-50 µg/mL penicillin-streptomycin, 2 mM L-glutamine, and 50 µg/mL Geneticin in a humidified atmosphere of 5% CO2-95% air. CHO cells were grown to a confluence in

150-mL flasks and were detached into Ham’s F-12 medium after treatment with 0.05% trypsin-0.53 mM EDTA solution. The cells were then pelleted and washed 3 times with TrisEDTA buffer (50 mM Tris-HCl, 150 mM NaCl, 20 mM EDTA, pH 7.4) and resuspended in the same buffer. Competition binding experiments were performed in duplicate using these whole cells. Aliquots (150 µL) of cells were added to tubes containing 50 µL of [125I]ICYP [(1.5-5) × 104 cells/20-60 pM ICYP] and varying concentrations of competing drugs. The final volume in each tube was 0.25 mL. Nonspecific binding (5-30%) was determined in the presence of 1 µM (()propranolol. Incubations were carried out for 60 min at 37 °C. Binding reactions were terminated by rapid filtration through Whatman GF/B glass fiber filters on a Brandel Model 12-R tissue harvester. Filters were washed twice with icecold Tris-EDTA buffer to remove free ICYP. The filters were dried under tissue harvester vacuum, and radioactivity was measured by gamma scintillation spectrometry (Beckman Model 8000 gamma counter; Palo Alto, CA). Specific binding to β-AR in these cells varied from 94 to 100%. Thromboxane A2/Prostaglandin H2 (TP) Receptor Sites in Human Platelets. For binding experiments, human platelet-rich plasma (PRP) was centrifuged and resuspended in 50 mM Tris-saline buffer, pH 7.4.9 Platelets were incubated with 5 nM [3H]-SQ 29548 in a final volume of 0.5 mL as described by Hedberg et al.21 Unlabeled SQ 29548 (50 µM) was used to determine nonspecific binding. Varying concentrations of each competing drug were used to quantify the inhibition of specific [3H]-SQ 29548 binding. Samples were incubated 30 min at room temperature, and rapidly filtered by vacuum through Whatman GF/C glass fiber filters on a Brandel cell harvester and washed for 10 s with ice-cold Trissaline buffer. Filters were placed in plastic scintillation vials containing 10 mL of an emulsion-type scintillation mixture, and radioactivity was measured by liquid scintillation spectrometry. Specific binding to human platelets varied between 88 and 95%. Data Analysis. Competitive binding data were analyzed using the PC-version of the radioligand binding program LIGAND (McPherson, 1985). Inhibitory concentration-50 (IC50) value of each competing drug was determined graphically from individual plots of percent radioligand bound versus log drug concentration on β-adrenoceptors and human platelets. Dissociation constants (Ki) for each competing drug were calculated using the equation: Ki ) IC50/(1 + [L]/KL) and the data expressed as pKi (i.e., -log Ki) values. The KL values used in the above equation are 17 pM, 10 pM, and 3.1 nM for β1-AR, β2-AR, and TP receptors, respectively.

Acknowledgment. The authors thank the National Institutes of Health (USPHS Grant HL-22533) for support of this work. References (1) Iwasawa, Y.; Kiyomoto, A. Studies of tetrahydroisoquinolines (THI) 1. Bronchodilator activity and structure-activity relationships. Jpn. J. Pharmacol. 1967, 17, 143-152. (2) Yamamoto, E.; Hirakura, M.; Sugasawa, S. Synthesis of 6,7dihydroxy-1,2,3,4-tetrahydroisoquinoline derivatives. Tetrahedron Suppl. 1966, 8 (Part 1), 129-134. (3) Mayo, J. R.; Navaran, S. S.; Akbar, H.; Miller, D. D.; Feller, D. R. Stereodependent inhibition of human platelet function by the optical isomers of trimethoquinol. Biochem. Pharmacol. 1981, 30, 2237-2241. (4) Ahn, C. H.; Romstedt, K. J.; Wallace, L. J.; Miller, D. D.; Feller, D. R. Characterization of the inhibition of U46619-mediated human platelet activation by the trimetoquinol isomers. Evidence for endoperoxide/thromboxane A2 receptor blockade. Biochem. Pharmacol. 1988, 37, 3023-3033. (5) Shin, Y.; Romstedt, K. J.; Miller, D. D.; Feller, D. R. Sterodependent antagonism of thromboxane A2/prostaglandin H2 receptor sites by trimetoquinol isomers in human platelets, rat vascular endothelial cells and rat vascular smooth muscle cells. Pharmacol. Commun. 1993, 1, 303-312. (6) Clark, M. T.; Adejare, A.; Shams, G.; Feller, D. R.; Miller, D. D. 5-Fluoro- and 8-fluorotrimetoquinol: selective beta 2-adrenoceptor agonists. J. Med. Chem. 1987, 30, 86-90.

Iodinated Analogs of Trimetoquinol (7) Fraundorfer, P. F. Functional and biochemical Characterization of trimetoquinol (TMQ) analog interactions with β-adrenergic receptor subtypes. Ph.D. Thesis, The Ohio State University, 1993. (8) Fraundorfer, P. F.; Fertel, R. H.; Miller, D. D.; Feller, D. R. Biochemical and pharmacological characterization of highaffinity trimetoquinol analogs on guinea pig and human beta adrenergic receptor subtypes: evidence for partial agonism. J. Pharmacol. Exp. Ther. 1994, 270, 665-674. (9) Shin, Y.; Romstedt, K. J.; Miller, D. D.; Feller, D. R. Interactions of nonprostanoid trimetoquinol analogs with thromboxane A2/ prostaglandin H2 receptors in human platelets, rat vascular endothelial cells and rat vascular smooth muscle cells. J. Pharmacol. Exp. Ther. 1993, 267, 1017-1023. (10) Harrold, M. W.; Gerhardt, M. A.; Romstedt, K.; Feller, D. R.; Miller, D. D. Synthesis and platelet antiaggregatory activity of trimetoquinol analogs as endoperoxide/thromboxane A2 antagonists. Drug Des. Delivery 1987, 1, 193-207. (11) Howe, R. Beta-3 adrenergic agonists. Drugs Future 1993, 18, 529-549. (12) Shin, Y.; Romstedt, K.; Doyle, K.; Harrold, M.; Gerhardt, M.; Miller, D.; Feller, D. Pharmacologic antagonism of thromboxane A2 receptors by trimetoquinol analogs. Chirality 1991, 3, 112117. (13) Adejare, A.; Miller, D. D.; Fedyna, J. S.; Ahn, C. H.; Feller, D. R. Syntheses and beta-adrenergic agonist and antiaggregatory properties of N-substituted trimetoquinol analogues. J. Med. Chem. 1986, 29, 1603-1609. (14) Kajigaeshi, S.; Kakinami, H.; Fujisaki, S.; Okamoto, T. Halogenation using quaternary ammonium polyhalides. VII. Iodination of aromatic amines by use of benzyltrimethylammonium dichloroiodate (I-). Bull. Chem. Soc. Jpn. 1968, 61, 600-602.

Journal of Medicinal Chemistry, 1996, Vol. 39, No. 19 3711 (15) Ulffers, F.; von Janson, A. Diacetylderivate einger Amine der aromatischen Reihe. Ber. Otsch. Chem. Ges. 1894, 27, 93-101. (16) Lott, R. S.; Chauhan, V. S.; Stammer, C. H. Trimethylsilyl iodide as a peptide deblocking agent. J. Chem. Soc., Chem. Commun. 1979, 495-496. (17) Dalton, D. R.; Cava, M. P.; Buck, K. T. Hindered rotation in 1-benzyl-1,2,3,4-tetrahydro-6,7-dimethoxyisoquinolines. Tetrahedron Lett. 1965, 2687-2690. (18) Tomita, M.; Shingu, T.; Fujitani, K.; Furikawa, H. Studies on the alkaloids of menispermaceous plants. CCXVI. Nuclear magnetic resonance spectra of benzylisoquinoline derivatives. (1). N-Methylcoclaurine type bases. Chem. Pharm. Bull. 1965, 13, 921-926. (19) Iwasa, K.; Kamigauchi, M.; Takao, N. Metabolism of salsalinol by tissue cultures of some Papaveraceae. Phytochemistry 1991, 30, 2973-2975. (20) Christoff, J. J. Part 1: Synthesis of arylalkylguanidines as dopamine agonists. Part 2, Section A: Modifications of trimetoquinol and the effects on beta-adrenergic and thromboxane A2 receptor system. Section B: Approaches to the asymmetric synthesis of irreversibly binding iodinated derivatives of trimetoquinol. Ph.D. Thesis, The Ohio State University, 1993. (21) Hedberg, A.; Hall, S.; Ogletree, M.; Harris, D.; Liu, E. Characterization of [5-63H]SQ 29,548 as a high affinity ligand for thromboxane A2/prostaglandin H2 receptors in human platelets. J. Pharmacol. Exp. Ther. 1988, 245, 786-792. (22) Viel, C.; Dorme, R.; Rumpf, P. Synthese et proprietes de nouvelles dimethoxy-6,7-dihydro-3,4-outetrahydr-1,2,3-isoquinoleines. Bull. Soc. Chim. Fr. 1966, 6, 1956-1966.

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